Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005947A_C01 KMC005947A_c01
(487 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_199962.1| pectinesterase family; protein id: At5g51490.1 ... 87 1e-16
ref|NP_199963.1| pectinesterase family; protein id: At5g51500.1 ... 80 2e-14
pir||T10494 pectinesterase (EC 3.1.1.11) PECS-c2 - sweet orange ... 80 2e-14
sp|O04887|PME2_CITSI Pectinesterase 2.1 precursor (Pectin methyl... 80 2e-14
ref|NP_190324.1| pectinesterase-like protein; protein id: At3g47... 73 1e-12
>ref|NP_199962.1| pectinesterase family; protein id: At5g51490.1 [Arabidopsis
thaliana] gi|9758191|dbj|BAB08665.1| pectinesterase
[Arabidopsis thaliana]
Length = 536
Score = 87.0 bits (214), Expect = 1e-16
Identities = 43/111 (38%), Positives = 65/111 (57%), Gaps = 4/111 (3%)
Frame = +2
Query: 164 WCNLTPHPEPCKHYITHMNPHSTIKHKTEFRGMLVQLALKQALNMQREAHDSQQNMVTDK 343
WC+ TP+P+PCK Y + N +EFR MLV+ A+ +A++ + E +S +N K
Sbjct: 37 WCDKTPYPDPCKCYFKNHNGFQQPTQLSEFRVMLVEAAMDRAISARAELTNSGKNCTDSK 96
Query: 344 HRTVHGDCLKLYENTIFHLNRTHHSLHLEKGSSPD----DTQTWLAXSLTN 484
+ V DC+ LY +TI LNRT H + + G++ D QTWL+ +LTN
Sbjct: 97 KQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAKSCTDFDAQTWLSTALTN 147
>ref|NP_199963.1| pectinesterase family; protein id: At5g51500.1 [Arabidopsis
thaliana] gi|9758192|dbj|BAB08666.1| pectinesterase
[Arabidopsis thaliana]
Length = 540
Score = 79.7 bits (195), Expect = 2e-14
Identities = 45/139 (32%), Positives = 74/139 (52%), Gaps = 2/139 (1%)
Frame = +2
Query: 74 IFLTLLWLPSIFSIASSRREPSPSSSNIGWWCNLTPHPEPCKHYITHMNPHSTIKHKTEF 253
+ + LL L + ++A +S+NI WC+ TP+P PCK Y + +EF
Sbjct: 17 LLILLLCLRPLTTVADG------NSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEF 70
Query: 254 RGMLVQLALKQALNMQREAHDSQQNMVTDKHRTVHGDCLKLYENTIFHLNRTHHSLHLEK 433
R +LV+ A+ +A++ + +S +N K + V DC+ LY +T+ LNRT + +
Sbjct: 71 RVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKT 130
Query: 434 G--SSPDDTQTWLAXSLTN 484
G + D QTWL+ +LTN
Sbjct: 131 GRRCTDFDAQTWLSTALTN 149
>pir||T10494 pectinesterase (EC 3.1.1.11) PECS-c2 - sweet orange
gi|2098713|gb|AAB57671.1| pectinesterase [Citrus
sinensis]
Length = 510
Score = 79.7 bits (195), Expect = 2e-14
Identities = 41/128 (32%), Positives = 71/128 (55%)
Frame = +2
Query: 104 IFSIASSRREPSPSSSNIGWWCNLTPHPEPCKHYITHMNPHSTIKHKTEFRGMLVQLALK 283
+FS++ + SP + WC TP+P+PC++++T ++IK T+F + +QLAL+
Sbjct: 13 LFSLSHTSFGYSPEE--VKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISLQLALE 70
Query: 284 QALNMQREAHDSQQNMVTDKHRTVHGDCLKLYENTIFHLNRTHHSLHLEKGSSPDDTQTW 463
+A Q + ++ + DC +LYE T+ LN+T +S G + D QTW
Sbjct: 71 RATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNS---SPGCTKVDKQTW 127
Query: 464 LAXSLTNI 487
L+ +LTN+
Sbjct: 128 LSSALTNL 135
>sp|O04887|PME2_CITSI Pectinesterase 2.1 precursor (Pectin methylesterase) (PE)
gi|7447379|pir||T10491 pectinesterase (EC 3.1.1.11)
PECS2.1 - sweet orange gi|2098709|gb|AAB57669.1|
pectinesterase [Citrus sinensis]
Length = 510
Score = 79.7 bits (195), Expect = 2e-14
Identities = 41/128 (32%), Positives = 71/128 (55%)
Frame = +2
Query: 104 IFSIASSRREPSPSSSNIGWWCNLTPHPEPCKHYITHMNPHSTIKHKTEFRGMLVQLALK 283
+FS++ + SP + WC TP+P+PC++++T ++IK T+F + +QLAL+
Sbjct: 13 LFSLSHTSFGYSPEE--VKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISLQLALE 70
Query: 284 QALNMQREAHDSQQNMVTDKHRTVHGDCLKLYENTIFHLNRTHHSLHLEKGSSPDDTQTW 463
+A Q + ++ + DC +LYE T+ LN+T +S G + D QTW
Sbjct: 71 RATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNS---SPGCTKVDKQTW 127
Query: 464 LAXSLTNI 487
L+ +LTN+
Sbjct: 128 LSTALTNL 135
>ref|NP_190324.1| pectinesterase-like protein; protein id: At3g47400.1 [Arabidopsis
thaliana] gi|7447382|pir||T12995 pectinesterase homolog
T21L8.150 - Arabidopsis thaliana
gi|5541707|emb|CAB51212.1| pectinesterase-like protein
[Arabidopsis thaliana]
Length = 594
Score = 73.2 bits (178), Expect = 1e-12
Identities = 39/122 (31%), Positives = 64/122 (51%), Gaps = 8/122 (6%)
Frame = +2
Query: 146 SSNIGWWCNLTPHPEPCKHYITHMNPHS----TIKHKTEFRGMLVQLALKQALNMQREAH 313
S ++ WWCN TPH E C +Y + ++ + ++EF MLV++AL QA+ +
Sbjct: 81 SGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTV 140
Query: 314 DSQQNMVTDKHRTVHGDCLKLYENTIFHLNRTHHSLHLEKGS----SPDDTQTWLAXSLT 481
+ ++ + DC+ L++NT+ LNRT L+ S + D QTWL+ + T
Sbjct: 141 KFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQT 200
Query: 482 NI 487
NI
Sbjct: 201 NI 202
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 431,471,539
Number of Sequences: 1393205
Number of extensions: 9308690
Number of successful extensions: 24195
Number of sequences better than 10.0: 29
Number of HSP's better than 10.0 without gapping: 23489
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24175
length of database: 448,689,247
effective HSP length: 113
effective length of database: 291,257,082
effective search space used: 13980339936
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
