Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005509A_C01 KMC005509A_c01
(498 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAB50687.2| parotid 'o' protein; Po [Homo sapiens] 44 0.001
ref|NP_005057.1| splicing factor proline/glutamine rich (polypyr... 44 0.001
ref|NP_076092.1| splicing factor proline/glutamine rich (polypyr... 43 0.002
gb|EAA11218.1| agCP5949 [Anopheles gambiae str. PEST] 42 0.005
pir||T28682 hypothetical protein - Streptomyces coelicolor (frag... 41 0.008
>gb|AAB50687.2| parotid 'o' protein; Po [Homo sapiens]
Length = 238
Score = 43.5 bits (101), Expect = 0.001
Identities = 38/115 (33%), Positives = 44/115 (38%), Gaps = 24/115 (20%)
Frame = +1
Query: 160 EGR---GGRKPERKKEPPSKQLHQNQRGPPP-----IQGTEPPWEEPSSHQKQ------- 294
EGR GG +P+R PP K +GPPP QG PP +P Q
Sbjct: 7 EGRRPQGGNQPQRPPPPPGK-----PQGPPPQGGNQSQGPPPPPGKPEGRPPQGGNQSQG 61
Query: 295 PTQRPCKEKRSP--------GTTQTLGKP-GKRKNGRNLRSSAPPHHKIPHLAAP 432
P P K +R P GT GKP G+ G N PPH P P
Sbjct: 62 PPPHPGKPERPPPQGGNQSQGTPPPPGKPEGRPPQGGNQSQGPPPHPGKPERPPP 116
Score = 35.0 bits (79), Expect = 0.47
Identities = 22/76 (28%), Positives = 31/76 (39%), Gaps = 3/76 (3%)
Frame = +1
Query: 160 EGRGGRKPERKKEPPSKQLHQNQRGPPP---IQGTEPPWEEPSSHQKQPTQRPCKEKRSP 330
+ R R P K + P +Q +GPPP QG PP P Q P +P + P
Sbjct: 163 KSRSARSPPGKPQGPPQQEGNKPQGPPPPGKPQGPPPPGGNPQQPQAPPAGKP----QGP 218
Query: 331 GTTQTLGKPGKRKNGR 378
G+P + G+
Sbjct: 219 PPPPQGGRPPRPAQGQ 234
>ref|NP_005057.1| splicing factor proline/glutamine rich (polypyrimidine tract
binding protein associated); splicing factor
proline/glutamine rich (polypyrimidine tract-binding
protein-associated); PTB-associated splicing factor
[Homo sapiens] gi|1709851|sp|P23246|SFPQ_HUMAN Splicing
factor, proline-and glutamine-rich (Polypyrimidine
tract-binding protein-associated splicing factor)
(PTB-associated splicing factor) (PSF) (DNA-binding
P52/P100 complex, 100 kDa subunit) gi|423054|pir||A46302
PTB-associated splicing factor, long form - human
gi|38458|emb|CAA50283.1| PTB-associated splicing factor
[Homo sapiens]
Length = 707
Score = 43.5 bits (101), Expect = 0.001
Identities = 24/65 (36%), Positives = 33/65 (49%)
Frame = +1
Query: 163 GRGGRKPERKKEPPSKQLHQNQRGPPPIQGTEPPWEEPSSHQKQPTQRPCKEKRSPGTTQ 342
G+ G KP PP HQ Q+ PPP Q PP ++P HQ P +P ++++ P Q
Sbjct: 52 GQSGPKPPIPPPPP----HQQQQQPPPQQ---PPPQQPPPHQPPPHPQPHQQQQPPPPPQ 104
Query: 343 TLGKP 357
KP
Sbjct: 105 DSSKP 109
Score = 33.9 bits (76), Expect = 1.0
Identities = 25/62 (40%), Positives = 32/62 (51%), Gaps = 6/62 (9%)
Frame = +1
Query: 163 GRGGRKPERKKEPPSKQLHQNQ--RGPPPIQ-GTEPPWEEPSSHQKQ---PTQRPCKEKR 324
GRGG R PP L+QN+ GP P Q G +PP P HQ+Q P Q+P ++
Sbjct: 24 GRGGLHDFRSP-PPGMGLNQNRGPMGPGPGQSGPKPPIPPPPPHQQQQQPPPQQPPPQQP 82
Query: 325 SP 330
P
Sbjct: 83 PP 84
Score = 33.1 bits (74), Expect = 1.8
Identities = 19/63 (30%), Positives = 26/63 (41%), Gaps = 2/63 (3%)
Frame = +1
Query: 175 RKPERKKEPPSKQLHQNQRGPPPIQGTEPP--WEEPSSHQKQPTQRPCKEKRSPGTTQTL 348
++P + PP Q HQ Q+ PPP Q + P + P + P P T T
Sbjct: 80 QQPPPHQPPPHPQPHQQQQPPPPPQDSSKPVVAQGPGPAPGVGSAPPASSSAPPATPPTS 139
Query: 349 GKP 357
G P
Sbjct: 140 GAP 142
>ref|NP_076092.1| splicing factor proline/glutamine rich (polypyrimidine tract
binding protein associated); DNA segment, Chr 4, ERATO
Doi 314, expressed [Mus musculus]
gi|25055901|ref|XP_204051.1| splicing factor
proline/glutamine rich (polypyrimidine tract binding
protein associated) [Mus musculus]
gi|17136071|gb|AAK60397.1| PTB-associated splicing
factor [Mus musculus]
Length = 699
Score = 43.1 bits (100), Expect = 0.002
Identities = 30/90 (33%), Positives = 42/90 (46%)
Frame = +1
Query: 163 GRGGRKPERKKEPPSKQLHQNQRGPPPIQGTEPPWEEPSSHQKQPTQRPCKEKRSPGTTQ 342
G GG KP PP HQ Q+ PPP Q PP ++P HQ+ P +P ++ P +
Sbjct: 50 GPGGPKPPLPPPPP----HQQQQQPPPQQ---PPPQQPPPHQQPPPHQPPHQQPPPPPQE 102
Query: 343 TLGKPGKRKNGRNLRSSAPPHHKIPHLAAP 432
+ + SSAPP P ++AP
Sbjct: 103 SKPVVPQGPGSAPGVSSAPP----PAVSAP 128
Score = 35.4 bits (80), Expect = 0.36
Identities = 31/95 (32%), Positives = 38/95 (39%), Gaps = 5/95 (5%)
Frame = +1
Query: 163 GRGGRKPERKKEPPSKQLHQNQRGP--PPIQGTEPPWEEPSSHQKQ---PTQRPCKEKRS 327
GRGG R PP L+QN RGP P G +PP P HQ+Q P Q+P ++
Sbjct: 24 GRGGLHDFRSP-PPGMGLNQN-RGPMGPGPGGPKPPLPPPPPHQQQQQPPPQQPPPQQPP 81
Query: 328 PGTTQTLGKPGKRKNGRNLRSSAPPHHKIPHLAAP 432
P PP H+ PH P
Sbjct: 82 P-------------------HQQPPPHQPPHQQPP 97
>gb|EAA11218.1| agCP5949 [Anopheles gambiae str. PEST]
Length = 373
Score = 41.6 bits (96), Expect = 0.005
Identities = 22/85 (25%), Positives = 42/85 (48%), Gaps = 4/85 (4%)
Frame = +1
Query: 175 RKPERKKEPPSKQLHQNQRGPPPIQGTEPPWEEPSSHQKQPTQRPCKEKRSPGTTQTLGK 354
++ +++++PP +Q HQ Q PP Q PP ++ Q+ P Q+ ++ + P Q
Sbjct: 29 QQQQQQQQPPPQQ-HQQQHPPPQQQQPPPPQQQQQFQQQPPPQQQQQQFQQPPPQQQQRH 87
Query: 355 PGKRKNGRNLRSSAPP----HHKIP 417
P ++ + + PP HH+ P
Sbjct: 88 PPPQQQQQQFQQQQPPPQPQHHQPP 112
>pir||T28682 hypothetical protein - Streptomyces coelicolor (fragment)
Length = 191
Score = 40.8 bits (94), Expect = 0.008
Identities = 24/76 (31%), Positives = 34/76 (44%), Gaps = 1/76 (1%)
Frame = +1
Query: 166 RGGRKPERKKEPPSKQLHQNQRGPPPIQGTEPPWEEPSSHQKQPTQRPCKEKRSPGTTQT 345
R GR+P R E P +Q H + R P G P +P + P QRP + + + T
Sbjct: 44 RRGRRPRRDHELPGRQQHLHLRRHPGHHGRGPRQPQPPPGARPPGQRPARRRTAHRTPAR 103
Query: 346 LGKPG-KRKNGRNLRS 390
+PG R G R+
Sbjct: 104 RRRPGAARARGTRART 119
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 485,552,995
Number of Sequences: 1393205
Number of extensions: 12233261
Number of successful extensions: 59386
Number of sequences better than 10.0: 532
Number of HSP's better than 10.0 without gapping: 48061
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 57085
length of database: 448,689,247
effective HSP length: 114
effective length of database: 289,863,877
effective search space used: 14783057727
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
