Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005423A_C01 KMC005423A_c01
(478 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAO42232.1| putative family II extracellular lipase 1 (EXL1) ... 74 1e-28
gb|AAF79814.1|AC007396_7 T4O12.12 [Arabidopsis thaliana] 74 6e-28
ref|NP_565120.1| family II extracellular lipase 1 (EXL1); protei... 74 6e-28
ref|NP_565121.1| family II extracellular lipase 2 (EXL2); protei... 69 2e-27
pir||A86335 T20H2.9 protein - Arabidopsis thaliana gi|8778985|gb... 63 3e-23
>gb|AAO42232.1| putative family II extracellular lipase 1 (EXL1) [Arabidopsis
thaliana] gi|28827430|gb|AAO50559.1| putative family II
extracellular lipase 1 (EXL1) [Arabidopsis thaliana]
Length = 374
Score = 73.6 bits (179), Expect(3) = 1e-28
Identities = 31/49 (63%), Positives = 40/49 (81%)
Frame = +1
Query: 217 NLLASARCNFPPYGKDFKGGMPTGRFSNGKVPSDIIAEELGMKELLPAY 363
+++ ARC++ PYG DF GG+ TGRFSNGKVP DI+AEELG+K +PAY
Sbjct: 68 DMITEARCDYAPYGIDFDGGVATGRFSNGKVPGDIVAEELGIKPNIPAY 116
Score = 49.3 bits (116), Expect(3) = 1e-28
Identities = 23/45 (51%), Positives = 33/45 (73%), Gaps = 1/45 (2%)
Frame = +3
Query: 90 VFLLIFIVTLKTM-A*VKLPPNISVPALLVYGDSIVDTGNNDKQL 221
+F+L+ + T T A VK+P N +VPA++V+GDSIVD GNND +
Sbjct: 26 IFVLVLLSTTSTTNALVKIPKNTTVPAVIVFGDSIVDAGNNDDMI 70
Score = 45.1 bits (105), Expect(3) = 1e-28
Identities = 21/35 (60%), Positives = 26/35 (74%)
Frame = +2
Query: 368 DPKLQPHELPTGVCFASGGAGYDPVTSETAAAISL 472
+P L+P EL TGV FASGGAGY P+T++ A I L
Sbjct: 118 NPNLKPEELLTGVTFASGGAGYVPLTTKIAGGIPL 152
>gb|AAF79814.1|AC007396_7 T4O12.12 [Arabidopsis thaliana]
Length = 687
Score = 73.6 bits (179), Expect(3) = 6e-28
Identities = 31/49 (63%), Positives = 40/49 (81%)
Frame = +1
Query: 217 NLLASARCNFPPYGKDFKGGMPTGRFSNGKVPSDIIAEELGMKELLPAY 363
+++ ARC++ PYG DF GG+ TGRFSNGKVP DI+AEELG+K +PAY
Sbjct: 68 DMITEARCDYAPYGIDFDGGVATGRFSNGKVPGDIVAEELGIKPNIPAY 116
Score = 68.6 bits (166), Expect(3) = 4e-25
Identities = 29/44 (65%), Positives = 35/44 (78%)
Frame = +1
Query: 232 ARCNFPPYGKDFKGGMPTGRFSNGKVPSDIIAEELGMKELLPAY 363
ARCN+PPYG DF GG+PTGRF NGKV +D IA + G+K +PAY
Sbjct: 377 ARCNYPPYGIDFDGGIPTGRFCNGKVATDFIAGKFGIKPSIPAY 420
Score = 49.3 bits (116), Expect(3) = 6e-28
Identities = 23/45 (51%), Positives = 33/45 (73%), Gaps = 1/45 (2%)
Frame = +3
Query: 90 VFLLIFIVTLKTM-A*VKLPPNISVPALLVYGDSIVDTGNNDKQL 221
+F+L+ + T T A VK+P N +VPA++V+GDSIVD GNND +
Sbjct: 26 IFVLVLLSTTSTTNALVKIPKNTTVPAVIVFGDSIVDAGNNDDMI 70
Score = 47.4 bits (111), Expect(3) = 4e-25
Identities = 21/48 (43%), Positives = 30/48 (61%)
Frame = +3
Query: 105 FIVTLKTMA*VKLPPNISVPALLVYGDSIVDTGNNDKQLASKCSLQFP 248
+ + T A VK PPN + PA++V+GDSIVD GNND + + +P
Sbjct: 335 YTASTSTNALVKQPPNETTPAIIVFGDSIVDAGNNDDIMTTLARCNYP 382
Score = 42.4 bits (98), Expect(3) = 6e-28
Identities = 19/30 (63%), Positives = 24/30 (79%)
Frame = +2
Query: 368 DPKLQPHELPTGVCFASGGAGYDPVTSETA 457
+P L+P EL TGV FASGGAGY P+T++ A
Sbjct: 118 NPNLKPEELLTGVTFASGGAGYVPLTTKIA 147
Score = 39.7 bits (91), Expect(3) = 4e-25
Identities = 17/28 (60%), Positives = 22/28 (77%)
Frame = +2
Query: 368 DPKLQPHELPTGVCFASGGAGYDPVTSE 451
+P L+P +L TGV FASGGAGY P T++
Sbjct: 422 NPNLKPEDLLTGVTFASGGAGYVPFTTQ 449
>ref|NP_565120.1| family II extracellular lipase 1 (EXL1); protein id: At1g75880.1,
supported by cDNA: gi_15054381 [Arabidopsis thaliana]
gi|15054382|gb|AAK30016.1| family II lipase EXL1
[Arabidopsis thaliana]
Length = 375
Score = 73.6 bits (179), Expect(3) = 6e-28
Identities = 31/49 (63%), Positives = 40/49 (81%)
Frame = +1
Query: 217 NLLASARCNFPPYGKDFKGGMPTGRFSNGKVPSDIIAEELGMKELLPAY 363
+++ ARC++ PYG DF GG+ TGRFSNGKVP DI+AEELG+K +PAY
Sbjct: 68 DMITEARCDYAPYGIDFDGGVATGRFSNGKVPGDIVAEELGIKPNIPAY 116
Score = 49.3 bits (116), Expect(3) = 6e-28
Identities = 23/45 (51%), Positives = 33/45 (73%), Gaps = 1/45 (2%)
Frame = +3
Query: 90 VFLLIFIVTLKTM-A*VKLPPNISVPALLVYGDSIVDTGNNDKQL 221
+F+L+ + T T A VK+P N +VPA++V+GDSIVD GNND +
Sbjct: 26 IFVLVLLSTTSTTNALVKIPKNTTVPAVIVFGDSIVDAGNNDDMI 70
Score = 42.4 bits (98), Expect(3) = 6e-28
Identities = 19/30 (63%), Positives = 24/30 (79%)
Frame = +2
Query: 368 DPKLQPHELPTGVCFASGGAGYDPVTSETA 457
+P L+P EL TGV FASGGAGY P+T++ A
Sbjct: 118 NPNLKPEELLTGVTFASGGAGYVPLTTKIA 147
>ref|NP_565121.1| family II extracellular lipase 2 (EXL2); protein id: At1g75890.1,
supported by cDNA: gi_15054383 [Arabidopsis thaliana]
gi|15054384|gb|AAK30017.1| family II lipase EXL2
[Arabidopsis thaliana]
Length = 379
Score = 68.6 bits (166), Expect(3) = 2e-27
Identities = 29/44 (65%), Positives = 35/44 (78%)
Frame = +1
Query: 232 ARCNFPPYGKDFKGGMPTGRFSNGKVPSDIIAEELGMKELLPAY 363
ARCN+PPYG DF GG+PTGRF NGKV +D IA + G+K +PAY
Sbjct: 69 ARCNYPPYGIDFDGGIPTGRFCNGKVATDFIAGKFGIKPSIPAY 112
Score = 55.5 bits (132), Expect(3) = 2e-27
Identities = 25/57 (43%), Positives = 35/57 (60%)
Frame = +3
Query: 78 PYLSVFLLIFIVTLKTMA*VKLPPNISVPALLVYGDSIVDTGNNDKQLASKCSLQFP 248
P+ VF L+ + T A VK PPN + PA++V+GDSIVD GNND + + +P
Sbjct: 18 PFWCVFFLVLLCKTSTNALVKQPPNETTPAIIVFGDSIVDAGNNDDIMTTLARCNYP 74
Score = 39.7 bits (91), Expect(3) = 2e-27
Identities = 17/28 (60%), Positives = 22/28 (77%)
Frame = +2
Query: 368 DPKLQPHELPTGVCFASGGAGYDPVTSE 451
+P L+P +L TGV FASGGAGY P T++
Sbjct: 114 NPNLKPEDLLTGVTFASGGAGYVPFTTQ 141
>pir||A86335 T20H2.9 protein - Arabidopsis thaliana
gi|8778985|gb|AAF79900.1|AC022472_9 Contains a strong
similarity to Anther-specific proline-rich protein APG
precursor from Arabidopsis thaliana gi|728867 and
contains a Lipase/Acylhydrolase domain with GDSL-like
motif PF|00657. ESTs gb|AV531882, gb|AV533240,
gb|AV534374, gb|AV533394, gb|AV532582, gb|AV533541 come
from this gene
Length = 1137
Score = 62.8 bits (151), Expect(3) = 3e-23
Identities = 29/55 (52%), Positives = 39/55 (70%)
Frame = +1
Query: 214 SNLLASARCNFPPYGKDFKGGMPTGRFSNGKVPSDIIAEELGMKELLPAYLGSKI 378
+NL + N+ PYG DFK + TGRFSNG V SD +A+ +G+KE++PAYL KI
Sbjct: 229 NNLETKIKSNYRPYGMDFKFRVATGRFSNGMVASDYLAKYMGVKEIVPAYLDPKI 283
Score = 62.4 bits (150), Expect(3) = 7e-17
Identities = 31/56 (55%), Positives = 42/56 (74%), Gaps = 1/56 (1%)
Frame = +1
Query: 199 LATMTSNLLAS-ARCNFPPYGKDFKGGMPTGRFSNGKVPSDIIAEELGMKELLPAY 363
L T +NLL + +R NF PYG+DF +PTGRF NG+V SD++A LG+K+LLPA+
Sbjct: 847 LDTGNNNLLMTVSRGNFLPYGRDFPHRIPTGRFGNGRVLSDLVASGLGVKDLLPAF 902
Score = 53.1 bits (126), Expect(3) = 3e-23
Identities = 24/35 (68%), Positives = 29/35 (82%)
Frame = +2
Query: 368 DPKLQPHELPTGVCFASGGAGYDPVTSETAAAISL 472
DPK+QP++L TGV FASGGAGY+P TSE A AI +
Sbjct: 280 DPKIQPNDLLTGVSFASGGAGYNPTTSEAANAIPM 314
Score = 40.8 bits (94), Expect(3) = 2e-11
Identities = 21/55 (38%), Positives = 28/55 (50%)
Frame = +1
Query: 214 SNLLASARCNFPPYGKDFKGGMPTGRFSNGKVPSDIIAEELGMKELLPAYLGSKI 378
+NL +CNF PYGKDF G+ T AE LG+K ++PAY +
Sbjct: 600 NNLTTEMKCNFSPYGKDFPLGVAT-------------AEYLGVKPIVPAYFDPNV 641
Score = 36.2 bits (82), Expect(3) = 7e-17
Identities = 17/32 (53%), Positives = 20/32 (62%)
Frame = +2
Query: 371 PKLQPHELPTGVCFASGGAGYDPVTSETAAAI 466
P L+ EL TGVCFASGG+G D T+ I
Sbjct: 905 PFLKNSELATGVCFASGGSGLDKFTASIQGVI 936
Score = 34.3 bits (77), Expect(3) = 2e-11
Identities = 16/36 (44%), Positives = 24/36 (66%)
Frame = +2
Query: 365 WDPKLQPHELPTGVCFASGGAGYDPVTSETAAAISL 472
+DP +Q +L TGV FASGG+GY +T + + S+
Sbjct: 637 FDPNVQLEDLLTGVSFASGGSGYYHLTPKISRVKSM 672
Score = 33.9 bits (76), Expect(3) = 2e-11
Identities = 13/23 (56%), Positives = 18/23 (77%)
Frame = +3
Query: 144 PPNISVPALLVYGDSIVDTGNND 212
P N + PA+ +GDSI+DTGNN+
Sbjct: 578 PHNKTTPAVFFFGDSIIDTGNNN 600
Score = 33.5 bits (75), Expect(3) = 3e-23
Identities = 12/23 (52%), Positives = 18/23 (78%)
Frame = +3
Query: 144 PPNISVPALLVYGDSIVDTGNND 212
P N ++PA+ +GDS+ DTGNN+
Sbjct: 207 PENKTIPAVFFFGDSVFDTGNNN 229
Score = 28.9 bits (63), Expect(3) = 7e-17
Identities = 11/16 (68%), Positives = 15/16 (93%)
Frame = +3
Query: 165 ALLVYGDSIVDTGNND 212
A+L +GDSI+DTGNN+
Sbjct: 838 AVLAFGDSILDTGNNN 853
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 425,510,310
Number of Sequences: 1393205
Number of extensions: 9223724
Number of successful extensions: 27544
Number of sequences better than 10.0: 198
Number of HSP's better than 10.0 without gapping: 25938
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27505
length of database: 448,689,247
effective HSP length: 113
effective length of database: 291,257,082
effective search space used: 13106568690
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
