KMC005171A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC005171A_C01 KMC005171A_c01
atgtgaatatgcccaccatctagaattCTAGATAAGACGACTTCTTATTCATACACTTAC
ATTAAGTATAGAACAATCAAACATCATTTGTCTGCGGGTGCATTTCAAACAATTCAAGCT
GAAGAGGAAATAAAAATCACCAAAATTATGGAAAAACAAGGAGAATTGAACAATTACAGA
TAGCCATATAGATGCATGAGCAGTCTCAAATTTGGTAATGTAACTAATTTTCGACGTGGA
TTATCACTTAGGTAGTACCAAAGTTTGTTCAGGTTTTGGTTCTGGTACATTCAGGTTCAA
CTACTGCTGCTCCTCATAAGCATTCTCCTCACCCTCATCATAATCCTCCTCCTCGTCTGC
AGTTGCATCCTGATATTGCTGGTACTCTGCAACCAAATCGTTCATGTTGCTCTCTGCTTC
AGTGAACTCCATTTCGTCCATTCCTTCCCCTGTGTACCAGTGCAAGAAGGCCTTGCGCCG
GTACATTGCAGTGAACTGCTCACTCACCCTCCTGAACATCTCCTGAATTGATGTGGAGTT
ACCAATAAAAGTACTCGACATCTTCAGATTCTTGGGTGGAATATCGCACACACTCGACTT
CACGTTGTTAGGAATCCACTCAACAAAGTTACGAGGAATTCTTGTTCTGCACATTGATCA
TCTGTTCATCCAC


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC005171A_C01 KMC005171A_c01
         (673 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAO64344.1| putative beta-tubulin [Vigna radiata]                  203  2e-54
sp|Q39445|TBB_CICAR Tubulin beta chain gi|1403143|emb|CAA67056.1...   202  1e-53
dbj|BAA82637.1| Beta-tubulin [Zinnia elegans]                         199  4e-53
gb|AAD02498.1| beta tubulin 1 [Arabidopsis thaliana]                  200  9e-53
ref|NP_177706.1| tubulin beta-1 chain; protein id: At1g75780.1 [...   200  9e-53

>gb|AAO64344.1| putative beta-tubulin [Vigna radiata]
          Length = 153

 Score =  203 bits (517), Expect(2) = 2e-54
 Identities = 97/102 (95%), Positives = 100/102 (97%)
 Frame = -2

Query: 627 FVEWIPNNVKSSVCDIPPKNLKMSSTFIGNSTSIQEMFRRVSEQFTAMYRRKAFLHWYTG 448
           FVEWIPNNVKSSVCDIPPKNLKMSSTFIGNSTSI+EMFRRVSEQFTAMYRRKAFLHWYTG
Sbjct: 46  FVEWIPNNVKSSVCDIPPKNLKMSSTFIGNSTSIREMFRRVSEQFTAMYRRKAFLHWYTG 105

Query: 447 EGMDEMEFTEAESNMNDLVAEYQQYQDATADEEEDYDEGEEN 322
           EGMDEMEFTEAESNMNDLVAEYQQYQDATADEEED+DE  E+
Sbjct: 106 EGMDEMEFTEAESNMNDLVAEYQQYQDATADEEEDFDEEHED 147

 Score = 31.2 bits (69), Expect(2) = 2e-54
 Identities = 14/14 (100%), Positives = 14/14 (100%)
 Frame = -1

Query: 673 VDEQMINVQNKNSS 632
           VDEQMINVQNKNSS
Sbjct: 31  VDEQMINVQNKNSS 44

>sp|Q39445|TBB_CICAR Tubulin beta chain gi|1403143|emb|CAA67056.1| beta-tubulin [Cicer
           arietinum]
          Length = 449

 Score =  202 bits (515), Expect(2) = 1e-53
 Identities = 96/107 (89%), Positives = 102/107 (94%)
 Frame = -2

Query: 627 FVEWIPNNVKSSVCDIPPKNLKMSSTFIGNSTSIQEMFRRVSEQFTAMYRRKAFLHWYTG 448
           FVEWIPNNVKSSVCDIPPKNLKMSSTFIGNSTSIQEMFRRVSEQFTAM+RRKAFLHWYTG
Sbjct: 343 FVEWIPNNVKSSVCDIPPKNLKMSSTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTG 402

Query: 447 EGMDEMEFTEAESNMNDLVAEYQQYQDATADEEEDYDEGEENAYEEQ 307
           EGMDEMEFTEAESNMNDLVAEYQQYQDA A+EE++Y+E  E  Y+EQ
Sbjct: 403 EGMDEMEFTEAESNMNDLVAEYQQYQDAIAEEEDEYEEEGEEQYDEQ 449

 Score = 29.6 bits (65), Expect(2) = 1e-53
 Identities = 13/14 (92%), Positives = 14/14 (99%)
 Frame = -1

Query: 673 VDEQMINVQNKNSS 632
           VDEQ+INVQNKNSS
Sbjct: 328 VDEQIINVQNKNSS 341

>dbj|BAA82637.1| Beta-tubulin [Zinnia elegans]
          Length = 448

 Score =  199 bits (506), Expect(2) = 4e-53
 Identities = 93/107 (86%), Positives = 101/107 (93%)
 Frame = -2

Query: 627 FVEWIPNNVKSSVCDIPPKNLKMSSTFIGNSTSIQEMFRRVSEQFTAMYRRKAFLHWYTG 448
           FVEWIPNNVKS+VCDIPP  LKM+STFIGNSTSIQEMFRRVSEQFTAM+RRKAFLHWYTG
Sbjct: 341 FVEWIPNNVKSTVCDIPPTGLKMASTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTG 400

Query: 447 EGMDEMEFTEAESNMNDLVAEYQQYQDATADEEEDYDEGEENAYEEQ 307
           EGMDEMEFTEAESNMNDLV+EYQQYQDATAD+E +Y+E EE  YEE+
Sbjct: 401 EGMDEMEFTEAESNMNDLVSEYQQYQDATADDEGEYEEDEEGVYEEE 447

 Score = 31.2 bits (69), Expect(2) = 4e-53
 Identities = 14/14 (100%), Positives = 14/14 (100%)
 Frame = -1

Query: 673 VDEQMINVQNKNSS 632
           VDEQMINVQNKNSS
Sbjct: 326 VDEQMINVQNKNSS 339

>gb|AAD02498.1| beta tubulin 1 [Arabidopsis thaliana]
          Length = 448

 Score =  200 bits (509), Expect(2) = 9e-53
 Identities = 93/105 (88%), Positives = 100/105 (94%)
 Frame = -2

Query: 627 FVEWIPNNVKSSVCDIPPKNLKMSSTFIGNSTSIQEMFRRVSEQFTAMYRRKAFLHWYTG 448
           FVEWIPNNVKSSVCDIPP  +KM+STF+GNSTSIQEMFRRVSEQFTAM+RRKAFLHWYTG
Sbjct: 343 FVEWIPNNVKSSVCDIPPTGIKMASTFVGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTG 402

Query: 447 EGMDEMEFTEAESNMNDLVAEYQQYQDATADEEEDYDEGEENAYE 313
           EGMDEMEFTEAESNMNDLV+EYQQYQDATADEE++YDE EE  YE
Sbjct: 403 EGMDEMEFTEAESNMNDLVSEYQQYQDATADEEDEYDEEEEQVYE 447

 Score = 28.9 bits (63), Expect(2) = 9e-53
 Identities = 12/14 (85%), Positives = 14/14 (99%)
 Frame = -1

Query: 673 VDEQMINVQNKNSS 632
           VDEQ++NVQNKNSS
Sbjct: 328 VDEQILNVQNKNSS 341

>ref|NP_177706.1| tubulin beta-1 chain; protein id: At1g75780.1 [Arabidopsis
           thaliana] gi|135442|sp|P12411|TBB1_ARATH Tubulin beta-1
           chain gi|71590|pir||UBMUBM tubulin beta-1 chain -
           Arabidopsis thaliana gi|166922|gb|AAA32893.1| beta-1
           tubulin gi|8778821|gb|AAF26774.2|AC007396_23 T4O12.1
           [Arabidopsis thaliana]
           gi|9369357|gb|AAF87106.1|AC006434_2 F10A5.3 [Arabidopsis
           thaliana]
          Length = 447

 Score =  200 bits (509), Expect(2) = 9e-53
 Identities = 93/105 (88%), Positives = 100/105 (94%)
 Frame = -2

Query: 627 FVEWIPNNVKSSVCDIPPKNLKMSSTFIGNSTSIQEMFRRVSEQFTAMYRRKAFLHWYTG 448
           FVEWIPNNVKSSVCDIPP  +KM+STF+GNSTSIQEMFRRVSEQFTAM+RRKAFLHWYTG
Sbjct: 342 FVEWIPNNVKSSVCDIPPTGIKMASTFVGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTG 401

Query: 447 EGMDEMEFTEAESNMNDLVAEYQQYQDATADEEEDYDEGEENAYE 313
           EGMDEMEFTEAESNMNDLV+EYQQYQDATADEE++YDE EE  YE
Sbjct: 402 EGMDEMEFTEAESNMNDLVSEYQQYQDATADEEDEYDEEEEQVYE 446

 Score = 28.9 bits (63), Expect(2) = 9e-53
 Identities = 12/14 (85%), Positives = 14/14 (99%)
 Frame = -1

Query: 673 VDEQMINVQNKNSS 632
           VDEQ++NVQNKNSS
Sbjct: 327 VDEQILNVQNKNSS 340

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 585,887,374
Number of Sequences: 1393205
Number of extensions: 13151311
Number of successful extensions: 63341
Number of sequences better than 10.0: 1234
Number of HSP's better than 10.0 without gapping: 50257
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 59350
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 29421376608
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 MPD050b12_f AV773378 1 516
2 MF097e04_f BP033355 28 557
3 MFB063g01_f BP038589 30 445
4 SPD021g06_f BP045674 34 608
5 MPD022f01_f AV771528 100 568
6 SPD056d01_f BP048452 101 518
7 SPD038g04_f BP047047 170 675
8 MWL017f09_f AV768864 181 477




Lotus japonicus
Kazusa DNA Research Institute