Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005088A_C01 KMC005088A_c01
(593 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAB09799.1| hypothetical protein [Citrus x paradisi] 219 2e-56
pir||T14439 hypothetical protein - wild cabbage (fragment) gi|14... 211 4e-54
ref|NP_172648.1| glyoxalase I, putative (lactoylglutathione lyas... 208 4e-53
gb|AAL07227.1| putative lactoylglutathione lyase [Arabidopsis th... 208 4e-53
dbj|BAA36759.1| glyoxalase I [Oryza sativa (japonica cultivar-gr... 207 1e-52
>emb|CAB09799.1| hypothetical protein [Citrus x paradisi]
Length = 291
Score = 219 bits (559), Expect = 2e-56
Identities = 104/127 (81%), Positives = 119/127 (92%)
Frame = -1
Query: 593 DLERAINFYEKALGLKVLRKVDKPESKYTVAILGYAEEHESTVLELTYNYGITEYSKGSA 414
DL R+I FYEKALG+K+LR VDKPE KYT+A+LGYAEE ++TVLELTYNYG+TEY+KG+A
Sbjct: 163 DLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTKGNA 222
Query: 413 YAQIAIGTDDVYKSAEVVNLVAQELGGKITRQPGPIPGLNTKVTSFLDPDGWKTVLVDNE 234
YAQ+AI TDDVYKSAEVVNLV QELGGKITRQPG IPGLNTK+TSF+DPDGWKTVLVDNE
Sbjct: 223 YAQVAISTDDVYKSAEVVNLVTQELGGKITRQPGSIPGLNTKITSFVDPDGWKTVLVDNE 282
Query: 233 DFLKELK 213
DFLKE++
Sbjct: 283 DFLKEIQ 289
Score = 109 bits (273), Expect = 2e-23
Identities = 54/112 (48%), Positives = 72/112 (64%)
Frame = -1
Query: 593 DLERAINFYEKALGLKVLRKVDKPESKYTVAILGYAEEHESTVLELTYNYGITEYSKGSA 414
DL+R I FY + G+K+LRK D PE KY+ A LG+ E V+ELTYNYG+T Y G+
Sbjct: 33 DLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTG 92
Query: 413 YAQIAIGTDDVYKSAEVVNLVAQELGGKITRQPGPIPGLNTKVTSFLDPDGW 258
+ AI T+DVYK E N+ A+ GG +TR+PGP+ G T + DPDG+
Sbjct: 93 FGHFAIATEDVYKLVE--NIRAK--GGNVTREPGPLKGGTTHIAFVKDPDGY 140
>pir||T14439 hypothetical protein - wild cabbage (fragment)
gi|1469219|emb|CAA99233.1| protein similar to bacterial
YRN1 and HEAHIO proteins [Brassica oleracea]
Length = 259
Score = 211 bits (538), Expect = 4e-54
Identities = 97/126 (76%), Positives = 116/126 (91%)
Frame = -1
Query: 593 DLERAINFYEKALGLKVLRKVDKPESKYTVAILGYAEEHESTVLELTYNYGITEYSKGSA 414
DL+RAI FYEKALG+++LR++++PE KYT+ ++GYAEE+ES VLELTYNYG+TEY+KG+A
Sbjct: 130 DLDRAIKFYEKALGMRLLRRIERPEYKYTIGMMGYAEEYESIVLELTYNYGVTEYTKGNA 189
Query: 413 YAQIAIGTDDVYKSAEVVNLVAQELGGKITRQPGPIPGLNTKVTSFLDPDGWKTVLVDNE 234
YAQIAIGTDDVYKSAEVV + QELGGKITR+ GP+PGL TK+ SFLDPDGWKTVLVDNE
Sbjct: 190 YAQIAIGTDDVYKSAEVVKIANQELGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNE 249
Query: 233 DFLKEL 216
DFLKEL
Sbjct: 250 DFLKEL 255
Score = 104 bits (259), Expect = 1e-21
Identities = 52/116 (44%), Positives = 70/116 (59%)
Frame = -1
Query: 590 LERAINFYEKALGLKVLRKVDKPESKYTVAILGYAEEHESTVLELTYNYGITEYSKGSAY 411
L+R I FY + GLKVLRK D PE KY+ A LG+ E + V+ELTYNYG++ Y G+ +
Sbjct: 1 LDRTIQFYTECFGLKVLRKRDVPEEKYSNAFLGFGPETSNFVVELTYNYGVSSYDIGTGF 60
Query: 410 AQIAIGTDDVYKSAEVVNLVAQELGGKITRQPGPIPGLNTKVTSFLDPDGWKTVLV 243
AI T DV K E V + GG +TR+PGP+ G + + DPDG+ L+
Sbjct: 61 GHFAISTQDVSKMVEAV----RAKGGNVTREPGPVKGGGSVIAFVKDPDGYTFELI 112
>ref|NP_172648.1| glyoxalase I, putative (lactoylglutathione lyase); protein id:
At1g11840.1, supported by cDNA: 39107., supported by
cDNA: gi_11094298, supported by cDNA: gi_15810218,
supported by cDNA: gi_16226212, supported by cDNA:
gi_18377844, supported by cDNA: gi_20453274 [Arabidopsis
thaliana] gi|25350142|pir||F86252 hypothetical protein
[imported] - Arabidopsis thaliana
gi|3157947|gb|AAC17630.1| Similar to protein gb|Z74962
from Brassica oleracea which is similar to bacterial
YRN1 and HEAHIO proteins. ESTs gb|T21954, gb|T04283,
gb|Z37609, gb|N37366, gb|R90704, gb|F15500 and gb|F14353
come from this gene. [Arabidopsis thaliana]
gi|11094299|dbj|BAB17665.1| Glyoxalase I homolog
[Arabidopsis thaliana]
gi|16226213|gb|AAL16104.1|AF428272_1 At1g11840/F12F1_32
[Arabidopsis thaliana] gi|18377845|gb|AAL67109.1|
At1g11840/F12F1_32 [Arabidopsis thaliana]
gi|20453275|gb|AAM19876.1| At1g11840/F12F1_32
[Arabidopsis thaliana] gi|21593459|gb|AAM65426.1|
lactoylglutathione lyase-like protein [Arabidopsis
thaliana]
Length = 283
Score = 208 bits (530), Expect = 4e-53
Identities = 95/127 (74%), Positives = 116/127 (90%)
Frame = -1
Query: 593 DLERAINFYEKALGLKVLRKVDKPESKYTVAILGYAEEHESTVLELTYNYGITEYSKGSA 414
DL+RAI FYEKALG+++LRK+++PE KYT+ ++GYAEE+ES VLELTYNY +TEY+KG+A
Sbjct: 157 DLDRAIKFYEKALGMRLLRKIERPEYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNA 216
Query: 413 YAQIAIGTDDVYKSAEVVNLVAQELGGKITRQPGPIPGLNTKVTSFLDPDGWKTVLVDNE 234
YAQIAIGTDDVYKS EV+ +V QELGGKITR+ GP+PGL TK+ SFLDPDGWKTVLVDN+
Sbjct: 217 YAQIAIGTDDVYKSGEVIKIVNQELGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNK 276
Query: 233 DFLKELK 213
DFLKEL+
Sbjct: 277 DFLKELE 283
Score = 102 bits (255), Expect = 3e-21
Identities = 51/117 (43%), Positives = 71/117 (60%)
Frame = -1
Query: 593 DLERAINFYEKALGLKVLRKVDKPESKYTVAILGYAEEHESTVLELTYNYGITEYSKGSA 414
DL+R I FY + G+K+LRK D PE KY+ A LG+ E + V+ELTYNYG++ Y G+
Sbjct: 27 DLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPETSNFVVELTYNYGVSSYDIGTG 86
Query: 413 YAQIAIGTDDVYKSAEVVNLVAQELGGKITRQPGPIPGLNTKVTSFLDPDGWKTVLV 243
+ AI T DV K E V + GG +TR+PGP+ G + + DPDG+ L+
Sbjct: 87 FGHFAISTQDVSKLVENV----RAKGGNVTREPGPVKGGGSVIAFVKDPDGYTFELI 139
>gb|AAL07227.1| putative lactoylglutathione lyase [Arabidopsis thaliana]
Length = 283
Score = 208 bits (530), Expect = 4e-53
Identities = 95/127 (74%), Positives = 116/127 (90%)
Frame = -1
Query: 593 DLERAINFYEKALGLKVLRKVDKPESKYTVAILGYAEEHESTVLELTYNYGITEYSKGSA 414
DL+RAI FYEKALG+++LRK+++PE KYT+ ++GYAEE+ES VLELTYNY +TEY+KG+A
Sbjct: 157 DLDRAIKFYEKALGMRLLRKIERPEYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNA 216
Query: 413 YAQIAIGTDDVYKSAEVVNLVAQELGGKITRQPGPIPGLNTKVTSFLDPDGWKTVLVDNE 234
YAQIAIGTDDVYKS EV+ +V QELGGKITR+ GP+PGL TK+ SFLDPDGWKTVLVDN+
Sbjct: 217 YAQIAIGTDDVYKSGEVIKIVNQELGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNK 276
Query: 233 DFLKELK 213
DFLKEL+
Sbjct: 277 DFLKELE 283
Score = 99.8 bits (247), Expect = 2e-20
Identities = 50/117 (42%), Positives = 70/117 (59%)
Frame = -1
Query: 593 DLERAINFYEKALGLKVLRKVDKPESKYTVAILGYAEEHESTVLELTYNYGITEYSKGSA 414
DL+R I FY + G+K+L K D PE KY+ A LG+ E + V+ELTYNYG++ Y G+
Sbjct: 27 DLDRTIEFYTEVFGMKLLWKRDIPEEKYSNAFLGFGPETSNFVVELTYNYGVSSYDIGTG 86
Query: 413 YAQIAIGTDDVYKSAEVVNLVAQELGGKITRQPGPIPGLNTKVTSFLDPDGWKTVLV 243
+ AI T DV K E V + GG +TR+PGP+ G + + DPDG+ L+
Sbjct: 87 FGHFAISTQDVSKLVENV----RAKGGNVTREPGPVKGGGSVIAFVKDPDGYTFELI 139
>dbj|BAA36759.1| glyoxalase I [Oryza sativa (japonica cultivar-group)]
Length = 291
Score = 207 bits (526), Expect = 1e-52
Identities = 95/127 (74%), Positives = 115/127 (89%)
Frame = -1
Query: 593 DLERAINFYEKALGLKVLRKVDKPESKYTVAILGYAEEHESTVLELTYNYGITEYSKGSA 414
DL+R+I FYEKALG+K+LRK D P+ KYT+A+LGYA+E ++TV+ELTYNYG+TEY+KG+A
Sbjct: 165 DLDRSIKFYEKALGMKLLRKKDVPDYKYTIAMLGYADEDKTTVIELTYNYGVTEYTKGNA 224
Query: 413 YAQIAIGTDDVYKSAEVVNLVAQELGGKITRQPGPIPGLNTKVTSFLDPDGWKTVLVDNE 234
YAQ+AIGT+DVYKSAE V LV +ELGGKI RQPGP+PGLNTK+ SFLDPDGWK VLVDN
Sbjct: 225 YAQVAIGTEDVYKSAEAVELVTKELGGKILRQPGPLPGLNTKIASFLDPDGWKVVLVDNA 284
Query: 233 DFLKELK 213
DFLKEL+
Sbjct: 285 DFLKELQ 291
Score = 104 bits (259), Expect = 1e-21
Identities = 53/117 (45%), Positives = 71/117 (60%)
Frame = -1
Query: 593 DLERAINFYEKALGLKVLRKVDKPESKYTVAILGYAEEHESTVLELTYNYGITEYSKGSA 414
DL+R I Y + G+K+LRK D PE KYT A LG+ E + LELTYNYG+ +Y G+
Sbjct: 34 DLDRTIKCYTECFGMKLLRKRDVPEEKYTNAFLGFGPEDTNFALELTYNYGVDKYDIGAG 93
Query: 413 YAQIAIGTDDVYKSAEVVNLVAQELGGKITRQPGPIPGLNTKVTSFLDPDGWKTVLV 243
+ AI T+DVYK AE + KITR+PGP+ G +T + DPDG+ L+
Sbjct: 94 FGHFAIATEDVYKLAEKIK---SSCCCKITREPGPVKGGSTVIAFAQDPDGYMFELI 147
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 471,671,462
Number of Sequences: 1393205
Number of extensions: 9857711
Number of successful extensions: 25886
Number of sequences better than 10.0: 146
Number of HSP's better than 10.0 without gapping: 24772
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25786
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 22854740960
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
