KMC004882A_c02
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC004882A_C02 KMC004882A_c02
ttttttttTTTTTTTTTAGACAGAGAACAATGAACTTCTATTACCATGAACCTCATCAAA
CGATGGTTACAGTAGGATCCGCTCCCGACTTGAACCTGATTTGTGTTATGAGTCACCCCA
TTAACACAAACAAAAACACAAATCAAACCAGCTTAATTTTTTCTCTTCCCAGTGCCCATT
TCCGGTTAAAAGCTCGGGGACTGGTTATCTAAATTCACACACAAGGATCCAAATTTCAGA
CCCTCTGGTTTCAGATTCGCCTATCTAACTATGAGTGATTTATACAAACCCCATTGATCT
CACCAAATACAATTTCAAACTAAGTGTCACGTTCATACTTCTATACTATGGCACTCAATC
TAGTGAACTACTTTCAGCTTTAACTATACTCCTTTTTATTACTCATTCCTTTTTTCTCCT
TGCAGTTTAAGTCACTACACACTACACTTAAAAGGGTGCTGCCTTTTATCAACCCCTCCA
ATTTGTTGGAGTGCAAACAGCTTCAACAGATAACCTCTTCATTACATTGGGACAAGGGTC
TTGCCCAAAGTTGCTGTTTGTTACAGTGACTATGCATTTTGGC


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004882A_C02 KMC004882A_c02
         (583 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAF70825.1|AF154424_1 putative beta-galactosidase [Lycopersic...    61  1e-08
ref|NP_568001.1| glycosyl hydrolase family 35 (beta-galactosidas...    60  3e-08
pir||B85429 beta-galactosidase like protein [imported] - Arabido...    60  3e-08
pir||T04600 probable beta-galactosidase (EC 3.2.1.23) F23E13.200...    60  3e-08
gb|AAK81874.1| putative beta-galactosidase BG1 [Vitis vinifera]        55  5e-07

>gb|AAF70825.1|AF154424_1 putative beta-galactosidase [Lycopersicon esculentum]
          Length = 845

 Score = 60.8 bits (146), Expect = 1e-08
 Identities = 25/31 (80%), Positives = 29/31 (92%)
 Frame = -2

Query: 576 CIVTVTNSNFGQDPCPNVMKRLSVEAVCTPT 484
           C VT++NSNFG+DPCPNV+KRLSVEA CTPT
Sbjct: 813 CAVTISNSNFGEDPCPNVLKRLSVEAHCTPT 843

>ref|NP_568001.1| glycosyl hydrolase family 35 (beta-galactosidase); protein id:
           At4g36360.1, supported by cDNA: gi_15810492, supported
           by cDNA: gi_20259270 [Arabidopsis thaliana]
           gi|6686878|emb|CAB64739.1| putative beta-galactosidase
           [Arabidopsis thaliana] gi|15810493|gb|AAL07134.1|
           putative beta-galactosidase [Arabidopsis thaliana]
           gi|20259271|gb|AAM14371.1| putative beta-galactosidase
           [Arabidopsis thaliana]
          Length = 856

 Score = 59.7 bits (143), Expect = 3e-08
 Identities = 25/39 (64%), Positives = 32/39 (81%), Gaps = 4/39 (10%)
 Frame = -2

Query: 579 KCIVTVTNSNFGQDPCPNVMKRLSVEAVCTP----TNWR 475
           +C VT++NSNFG+DPCPNV+KRL+VEAVC P    + WR
Sbjct: 817 RCAVTISNSNFGKDPCPNVLKRLTVEAVCAPETSVSTWR 855

>pir||B85429 beta-galactosidase like protein [imported] - Arabidopsis thaliana
           gi|4006924|emb|CAB16852.1| beta-galactosidase like
           protein [Arabidopsis thaliana]
           gi|7270584|emb|CAB80302.1| beta-galactosidase like
           protein [Arabidopsis thaliana]
          Length = 853

 Score = 59.7 bits (143), Expect = 3e-08
 Identities = 25/39 (64%), Positives = 32/39 (81%), Gaps = 4/39 (10%)
 Frame = -2

Query: 579 KCIVTVTNSNFGQDPCPNVMKRLSVEAVCTP----TNWR 475
           +C VT++NSNFG+DPCPNV+KRL+VEAVC P    + WR
Sbjct: 814 RCAVTISNSNFGKDPCPNVLKRLTVEAVCAPETSVSTWR 852

>pir||T04600 probable beta-galactosidase (EC 3.2.1.23) F23E13.200 - Arabidopsis
           thaliana gi|2961390|emb|CAA18137.1| beta-galactosidase
           like protein [Arabidopsis thaliana]
          Length = 853

 Score = 59.7 bits (143), Expect = 3e-08
 Identities = 25/39 (64%), Positives = 32/39 (81%), Gaps = 4/39 (10%)
 Frame = -2

Query: 579 KCIVTVTNSNFGQDPCPNVMKRLSVEAVCTP----TNWR 475
           +C VT++NSNFG+DPCPNV+KRL+VEAVC P    + WR
Sbjct: 814 RCAVTISNSNFGKDPCPNVLKRLTVEAVCAPETSVSTWR 852

>gb|AAK81874.1| putative beta-galactosidase BG1 [Vitis vinifera]
          Length = 854

 Score = 55.5 bits (132), Expect = 5e-07
 Identities = 23/32 (71%), Positives = 27/32 (83%)
 Frame = -2

Query: 579 KCIVTVTNSNFGQDPCPNVMKRLSVEAVCTPT 484
           KC V ++NS FG DPCPNV+KRLSVEA C+PT
Sbjct: 813 KCSVPISNSYFGADPCPNVLKRLSVEAACSPT 844

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 490,141,238
Number of Sequences: 1393205
Number of extensions: 10425707
Number of successful extensions: 20390
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 19917
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 20380
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21712003912
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 SPDL006e11_f BP052358 1 516
2 MPDL064d08_f AV779755 9 483
3 SPDL003d03_f BP052172 18 475
4 MFBL035g05_f BP043032 18 528
5 SPDL052h01_f BP055310 18 528
6 SPDL034c08_f BP054114 18 514
7 MF037d07_f BP030247 18 454
8 SPDL063c01_f BP055899 18 529
9 MF010d09_f BP028751 18 514
10 MPDL059a02_f AV779478 19 505
11 SPDL088d06_f BP057524 19 349
12 MRL016e06_f BP084535 19 484
13 SPDL081a10_f BP057027 20 529
14 SPDL066d01_f BP056098 20 516
15 SPDL013c12_f BP052786 20 475
16 SPDL029h08_f BP053842 20 486
17 SPDL055a05_f BP055444 20 516
18 SPDL074b06_f BP056563 21 528
19 SPDL089e07_f BP057593 21 587
20 SPDL087d11_f BP057460 21 516
21 MFBL011e08_f BP041818 21 422




Lotus japonicus
Kazusa DNA Research Institute