Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC004838A_C01 KMC004838A_c01
(1103 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_180832.1| 26S proteasome regulatory subunit (RPN2), putat... 482 e-135
ref|NP_171973.1| 26S proteasome regulatory subunit (RPN2), putat... 471 e-131
gb|AAH45463.1| Similar to proteasome (prosome, macropain) 26S su... 269 7e-71
gb|AAH47897.1| Similar to proteasome (prosome, macropain) 26S su... 256 4e-67
ref|NP_002798.2| proteasome 26S non-ATPase subunit 1; 26S protea... 256 4e-67
>ref|NP_180832.1| 26S proteasome regulatory subunit (RPN2), putative; protein id:
At2g32730.1, supported by cDNA: gi_20466789 [Arabidopsis
thaliana] gi|7486095|pir||T00795 26S proteasome
regulatory subunit [imported] - Arabidopsis thaliana
gi|2914700|gb|AAC04490.1| 26S proteasome regulatory
subunit (RPN2), putative [Arabidopsis thaliana]
gi|20466790|gb|AAM20712.1| 26S proteasome regulatory
subunit [Arabidopsis thaliana]
Length = 1004
Score = 482 bits (1241), Expect = e-135
Identities = 256/331 (77%), Positives = 274/331 (82%)
Frame = +1
Query: 109 MATTLVSSGGGMLAMLNESHLSLKLHALSNLNNLVDTFWPEISTSVPLIESLYEDEEFDQ 288
MAT +VSS GG+LAMLNE H LKLHALSNLNNLVD FWPEISTSVP+IESLYEDEEFD
Sbjct: 1 MATPMVSSAGGLLAMLNEPHPVLKLHALSNLNNLVDQFWPEISTSVPIIESLYEDEEFDL 60
Query: 289 HQRQLAALVVSKVFYYLGELDDSLSYALGAGPLFDVSEDSDYVHTLLVKAIDEYASLKSK 468
HQRQLAAL+VSKVFYYLGEL+DSLSYALGAGPLFDVSEDSDYVHTLL KAIDEYASL+SK
Sbjct: 61 HQRQLAALLVSKVFYYLGELNDSLSYALGAGPLFDVSEDSDYVHTLLAKAIDEYASLRSK 120
Query: 469 AAESGDTSIKSDPRLEAIVERMFDKCIMDGKYQQAMGTAIECRRLDKLEEAITKSDNVQG 648
A ES + + DPRLEAIVERM KCI DGKYQQAMG AIECRRLDKLEEAI KSDNVQG
Sbjct: 121 AVESNEM-VDIDPRLEAIVERMLGKCISDGKYQQAMGIAIECRRLDKLEEAIIKSDNVQG 179
Query: 649 TLSYCINVSHSFVNLREYRQEVLRLLVKVFQKLSSPDYLSICQCLMFLDEPEGVASILER 828
TLSYCINVSHSFVN REYR EVL LLVKV+QKL SPDYLSICQCLMFLDEP+GVASILE+
Sbjct: 180 TLSYCINVSHSFVNRREYRHEVLSLLVKVYQKLPSPDYLSICQCLMFLDEPQGVASILEK 239
Query: 829 LLRSENKYDALLAFQIAFDLVENEHQAFLLSVRDRLASPKLPSESAQPKPSDTGSTPSAS 1008
LLRSENK DALLA QIAFDLVENEHQAFLLSVRDRL +PK + A +T + +
Sbjct: 240 LLRSENKDDALLALQIAFDLVENEHQAFLLSVRDRLPAPKTRAVEA---TQAVETTIAPN 296
Query: 1009 ANAPDDVQMEDGDSASIVNVPEDPSEKMYAE 1101
N DVQM D A + DP + YAE
Sbjct: 297 ENPSGDVQMADETPAQTIVHETDPVDATYAE 327
>ref|NP_171973.1| 26S proteasome regulatory subunit (RPN2), putative; protein id:
At1g04810.1 [Arabidopsis thaliana]
gi|25347943|pir||C86181 hypothetical protein [imported] -
Arabidopsis thaliana gi|7211984|gb|AAF40455.1|AC004809_13
Strong similarity to the SEN3-like tRNA-processing
protein F24L7.13 from A. thaliana gi|2914700 on BAC
gb|AC003974. EST gb|AA712969 comes from this gene.
[Arabidopsis thaliana] gi|28393231|gb|AAO42045.1|
putative 26S proteasome regulatory subunit (RPN2)
[Arabidopsis thaliana]
Length = 1001
Score = 471 bits (1211), Expect = e-131
Identities = 248/332 (74%), Positives = 275/332 (82%), Gaps = 1/332 (0%)
Frame = +1
Query: 109 MATTLVSSGGGMLAMLNESHLSLKLHALSNLNNLVDTFWPEISTSVPLIESLYEDEEFDQ 288
MA +VSS GG+LAMLNE H SLKLHALS L LVD FWPEISTSVP+IESLYEDEEFDQ
Sbjct: 1 MAAAMVSSAGGLLAMLNEPHPSLKLHALSYLIRLVDQFWPEISTSVPIIESLYEDEEFDQ 60
Query: 289 HQRQLAALVVSKVFYYLGELDDSLSYALGAGPLFDVSEDSDYVHTLLVKAIDEYASLKSK 468
HQRQLAAL+ SKVFYYLGEL+DSLSYALGAG LFDVSEDSDY+HTLL KAIDEYA L+SK
Sbjct: 61 HQRQLAALLASKVFYYLGELNDSLSYALGAGSLFDVSEDSDYIHTLLSKAIDEYAILRSK 120
Query: 469 AAESGDTSIKSDPRLEAIVERMFDKCIMDGKYQQAMGTAIECRRLDKLEEAITKSDNVQG 648
A ES + ++ DPRL AIVERM DKCI DGKYQQAMG AIECRRLDKLEEAI KS+NVQG
Sbjct: 121 AVESSEV-VEIDPRLVAIVERMLDKCITDGKYQQAMGIAIECRRLDKLEEAIIKSENVQG 179
Query: 649 TLSYCINVSHSFVNLREYRQEVLRLLVKVFQKLSSPDYLSICQCLMFLDEPEGVASILER 828
TLSYCINVSHSFVN REYR EVLRLLV V+QKL+SPDYLSICQCLMFLDEP+GVASILE+
Sbjct: 180 TLSYCINVSHSFVNQREYRHEVLRLLVNVYQKLASPDYLSICQCLMFLDEPQGVASILEK 239
Query: 829 LLRSENKYDALLAFQIAFDLVENEHQAFLLSVRDRLASPKL-PSESAQPKPSDTGSTPSA 1005
LLRSENK DALLAFQI+FDLV+NEHQAFL+SVRDRL +PK P E+ Q + T +
Sbjct: 240 LLRSENKDDALLAFQISFDLVQNEHQAFLMSVRDRLPAPKTRPVEAIQAVETST----AQ 295
Query: 1006 SANAPDDVQMEDGDSASIVNVPEDPSEKMYAE 1101
+ N DVQM D + + DP + +YAE
Sbjct: 296 NENTAGDVQMADETPSQTIVHETDPVDAVYAE 327
>gb|AAH45463.1| Similar to proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
[Danio rerio]
Length = 887
Score = 269 bits (687), Expect = 7e-71
Identities = 146/333 (43%), Positives = 218/333 (64%), Gaps = 9/333 (2%)
Frame = +1
Query: 121 LVSSGGGMLAMLNESHLSLKLHALSNLNNLVDTFWPEISTSVPLIESLYEDEEFDQHQRQ 300
+++S G++++L+E LK AL LN++V+ FW EIS SV IE LYEDE F R+
Sbjct: 1 MITSAAGIISLLDEDEPQLKEFALHKLNSVVNDFWAEISESVGKIEVLYEDETF--RSRE 58
Query: 301 LAALVVSKVFYYLGELDDSLSYALGAGPLFDVSEDSDYVHTLLVKAIDEYASLKSKAAES 480
AALV SKVFY+LG D+SLSYALGAG LF+V++DS+YV T++ K ID Y + + AE
Sbjct: 59 FAALVASKVFYHLGAFDESLSYALGAGDLFNVNDDSEYVETIIAKCIDHYTKQRVENAEL 118
Query: 481 GDTSIKSD--PRLEAIVERMFDKCIMDGKYQQAMGTAIECRRLDKLEEAITKSDNVQGTL 654
+ K D PRLE IV +MF +C+ D KY+QA+G A+E RRLD E+ I +S+++ G L
Sbjct: 119 PEEEEKKDIDPRLEGIVNKMFQRCLGDHKYKQAIGIALETRRLDIFEKTILESNDIGGLL 178
Query: 655 SYCINVSHSFVNLREYRQEVLRLLVKVFQKLSSPDYLSICQCLMFLDEPEGVASILERLL 834
+Y + + S + +++R EVLR+LVK++ L PD++++CQCL+FLD+P+ V+ ILE+L+
Sbjct: 179 AYSLKICMSLMQNKKFRNEVLRVLVKLYMNLEKPDFINVCQCLIFLDDPQAVSDILEKLV 238
Query: 835 RSENKYDALLAFQIAFDLVENEHQAFLLSVRDRLASPKLPSESAQPKPSDTGSTPSASAN 1014
+ +N L+A+QI FDL E+ Q FL SV L + P A P ++TG+ P+ +
Sbjct: 239 KEDN---LLMAYQICFDLYESASQQFLSSVIQNLRTVGTPI-PAVPGSTNTGTVPTPDKD 294
Query: 1015 APDDVQMED-------GDSASIVNVPEDPSEKM 1092
+ D ++ ED G +A + P+D + KM
Sbjct: 295 S-DSMETEDKAGSSPAGKTADAKDEPKDQNSKM 326
>gb|AAH47897.1| Similar to proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
[Homo sapiens]
Length = 844
Score = 256 bits (654), Expect = 4e-67
Identities = 139/323 (43%), Positives = 207/323 (64%), Gaps = 1/323 (0%)
Frame = +1
Query: 121 LVSSGGGMLAMLNESHLSLKLHALSNLNNLVDTFWPEISTSVPLIESLYEDEEFDQHQRQ 300
+++S G++++L+E LK AL LN +V+ FW EIS SV IE LYEDE F RQ
Sbjct: 1 MITSAAGIISLLDEDEPQLKEFALHKLNAVVNDFWAEISESVDKIEVLYEDEGF--RSRQ 58
Query: 301 LAALVVSKVFYYLGELDDSLSYALGAGPLFDVSEDSDYVHTLLVKAIDEYASLKSKAAES 480
AALV SKVFY+LG ++SL+YALGAG LF+V+++S+YV T++ K ID Y + A+
Sbjct: 59 FAALVASKVFYHLGAFEESLNYALGAGDLFNVNDNSEYVETIIAKCIDHYTKQCVENADL 118
Query: 481 GDTSIKS-DPRLEAIVERMFDKCIMDGKYQQAMGTAIECRRLDKLEEAITKSDNVQGTLS 657
+ K D RLE IV +MF +C+ D KY+QA+G A+E RRLD E+ I +S++V G L+
Sbjct: 119 PEGEKKPIDQRLEGIVNKMFQRCLDDHKYKQAIGIALETRRLDVFEKTILESNDVPGMLA 178
Query: 658 YCINVSHSFVNLREYRQEVLRLLVKVFQKLSSPDYLSICQCLMFLDEPEGVASILERLLR 837
Y + + S + +++R +VLR+LVK++ L PD++++CQCL+FLD+P+ V+ ILE+L++
Sbjct: 179 YSLKLCMSLMQNKQFRNKVLRVLVKIYMNLEKPDFINVCQCLIFLDDPQAVSDILEKLVK 238
Query: 838 SENKYDALLAFQIAFDLVENEHQAFLLSVRDRLASPKLPSESAQPKPSDTGSTPSASANA 1017
+N L+A+QI FDL E+ Q FL SV L + P S P ++TG+ P + ++
Sbjct: 239 EDN---LLMAYQICFDLYESASQQFLSSVIQNLRTVGTPIASV-PGSTNTGTVPGSEKDS 294
Query: 1018 PDDVQMEDGDSASIVNVPEDPSE 1086
E SA + PE E
Sbjct: 295 DSMETEEKTSSAFVGKTPEASPE 317
>ref|NP_002798.2| proteasome 26S non-ATPase subunit 1; 26S proteasome non-ATPase
regulatory subunit 1; 26S proteasome regulatory subunit
S1; 26S proteasome subunit p112 [Homo sapiens]
Length = 953
Score = 256 bits (654), Expect = 4e-67
Identities = 139/323 (43%), Positives = 207/323 (64%), Gaps = 1/323 (0%)
Frame = +1
Query: 121 LVSSGGGMLAMLNESHLSLKLHALSNLNNLVDTFWPEISTSVPLIESLYEDEEFDQHQRQ 300
+++S G++++L+E LK AL LN +V+ FW EIS SV IE LYEDE F RQ
Sbjct: 1 MITSAAGIISLLDEDEPQLKEFALHKLNAVVNDFWAEISESVDKIEVLYEDEGF--RSRQ 58
Query: 301 LAALVVSKVFYYLGELDDSLSYALGAGPLFDVSEDSDYVHTLLVKAIDEYASLKSKAAES 480
AALV SKVFY+LG ++SL+YALGAG LF+V+++S+YV T++ K ID Y + A+
Sbjct: 59 FAALVASKVFYHLGAFEESLNYALGAGDLFNVNDNSEYVETIIAKCIDHYTKQCVENADL 118
Query: 481 GDTSIKS-DPRLEAIVERMFDKCIMDGKYQQAMGTAIECRRLDKLEEAITKSDNVQGTLS 657
+ K D RLE IV +MF +C+ D KY+QA+G A+E RRLD E+ I +S++V G L+
Sbjct: 119 PEGEKKPIDQRLEGIVNKMFQRCLDDHKYKQAIGIALETRRLDVFEKTILESNDVPGMLA 178
Query: 658 YCINVSHSFVNLREYRQEVLRLLVKVFQKLSSPDYLSICQCLMFLDEPEGVASILERLLR 837
Y + + S + +++R +VLR+LVK++ L PD++++CQCL+FLD+P+ V+ ILE+L++
Sbjct: 179 YSLKLCMSLMQNKQFRNKVLRVLVKIYMNLEKPDFINVCQCLIFLDDPQAVSDILEKLVK 238
Query: 838 SENKYDALLAFQIAFDLVENEHQAFLLSVRDRLASPKLPSESAQPKPSDTGSTPSASANA 1017
+N L+A+QI FDL E+ Q FL SV L + P S P ++TG+ P + ++
Sbjct: 239 EDN---LLMAYQICFDLYESASQQFLSSVIQNLRTVGTPIASV-PGSTNTGTVPGSEKDS 294
Query: 1018 PDDVQMEDGDSASIVNVPEDPSE 1086
E SA + PE E
Sbjct: 295 DSMETEEKTSSAFVGKTPEASPE 317
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.317 0.134 0.376
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 936,712,673
Number of Sequences: 1393205
Number of extensions: 20659284
Number of successful extensions: 70822
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 63659
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 70306
length of database: 448,689,247
effective HSP length: 125
effective length of database: 274,538,622
effective search space used: 66438346524
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
