Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC004651A_C01 KMC004651A_c01
(696 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAA75577.1| L-ascorbate oxidase [Medicago truncatula] 255 4e-67
gb|AAM94614.1| ascorbate oxidase precursor [Glycine max] 243 2e-63
gb|AAF20932.1|AF206722_1 ascorbate oxidase [Brassica juncea] 212 5e-54
gb|AAF20933.1|AF206723_1 ascorbate oxidase [Brassica juncea] 212 5e-54
ref|NP_680176.1| Expressed protein; protein id: At5g21105.1 [Ara... 208 7e-53
>emb|CAA75577.1| L-ascorbate oxidase [Medicago truncatula]
Length = 569
Score = 255 bits (652), Expect = 4e-67
Identities = 121/141 (85%), Positives = 129/141 (90%)
Frame = -3
Query: 694 NIFQSPVFRNTTVGNGVYMFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGE 515
+IF++PV NTT GNGVY FQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGE
Sbjct: 425 DIFKNPVNPNTTTGNGVYTFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGE 484
Query: 514 GKFRPGVDEKSFNLTRAPLRNTAVIFPYGWTALRFKAG*PLVFGRSHCHIEPHLHMGMGV 335
G+FRPGVDE+SFNLTRAPLRNTAVIFPYGWTALRFKA P V+ HCHIEPHLHMGMGV
Sbjct: 485 GRFRPGVDERSFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWA-FHCHIEPHLHMGMGV 543
Query: 334 IFAEAVHKVKNIPREALTCGL 272
IFAEAVHKV+NIP+EAL C L
Sbjct: 544 IFAEAVHKVRNIPKEALKCVL 564
>gb|AAM94614.1| ascorbate oxidase precursor [Glycine max]
Length = 429
Score = 243 bits (620), Expect = 2e-63
Identities = 118/146 (80%), Positives = 125/146 (84%)
Frame = -3
Query: 694 NIFQSPVFRNTTVGNGVYMFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGE 515
+IF PV N T+GNGVYMF LNEVVDVILQN+NQL+ NGSEIHPWHLHGHDFWVLGYGE
Sbjct: 280 DIFNPPVNPNATIGNGVYMFNLNEVVDVILQNSNQLSVNGSEIHPWHLHGHDFWVLGYGE 339
Query: 514 GKFRPGVDEKSFNLTRAPLRNTAVIFPYGWTALRFKAG*PLVFGRSHCHIEPHLHMGMGV 335
GKF+ G DEK FNLT APLRNTAVIFPYGWTALRFKA P V+ HCHIEPHLHMGMGV
Sbjct: 340 GKFKLG-DEKKFNLTHAPLRNTAVIFPYGWTALRFKADNPGVWA-FHCHIEPHLHMGMGV 397
Query: 334 IFAEAVHKVKNIPREALTCGLTGKMI 257
IFAE VHKV IPREALTCGLTGK +
Sbjct: 398 IFAEGVHKVGKIPREALTCGLTGKKL 423
>gb|AAF20932.1|AF206722_1 ascorbate oxidase [Brassica juncea]
Length = 574
Score = 212 bits (539), Expect = 5e-54
Identities = 99/152 (65%), Positives = 116/152 (76%)
Frame = -3
Query: 694 NIFQSPVFRNTTVGNGVYMFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGE 515
+I P RNTT GNG+Y+F N VDVILQNAN L+ N SEIHPWHLHGHDFWVLGYGE
Sbjct: 424 DIMNPPRNRNTTKGNGIYVFPFNVTVDVILQNANGLDANASEIHPWHLHGHDFWVLGYGE 483
Query: 514 GKFRPGVDEKSFNLTRAPLRNTAVIFPYGWTALRFKAG*PLVFGRSHCHIEPHLHMGMGV 335
GKFRPGVDEK++NL PL+NT +FPYGWTALRF P V+ HCHIEPHLHMGMGV
Sbjct: 484 GKFRPGVDEKTYNLKNPPLKNTVALFPYGWTALRFVTDNPGVW-FFHCHIEPHLHMGMGV 542
Query: 334 IFAEAVHKVKNIPREALTCGLTGKMIHH*N*P 239
+FAE ++++ IP EAL CGLT + + + N P
Sbjct: 543 VFAEGLNRIGKIPDEALGCGLTKQFLMNRNNP 574
>gb|AAF20933.1|AF206723_1 ascorbate oxidase [Brassica juncea]
Length = 573
Score = 212 bits (539), Expect = 5e-54
Identities = 99/152 (65%), Positives = 116/152 (76%)
Frame = -3
Query: 694 NIFQSPVFRNTTVGNGVYMFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGE 515
+I P RNTT GNG+Y+F N VDVILQNAN L+ N SEIHPWHLHGHDFWVLGYGE
Sbjct: 423 DIMNPPRNRNTTKGNGIYVFPFNVTVDVILQNANGLDANASEIHPWHLHGHDFWVLGYGE 482
Query: 514 GKFRPGVDEKSFNLTRAPLRNTAVIFPYGWTALRFKAG*PLVFGRSHCHIEPHLHMGMGV 335
GKFRPGVDEK++NL PL+NT +FPYGWTALRF P V+ HCHIEPHLHMGMGV
Sbjct: 483 GKFRPGVDEKTYNLKNPPLKNTVALFPYGWTALRFVTDNPGVW-FFHCHIEPHLHMGMGV 541
Query: 334 IFAEAVHKVKNIPREALTCGLTGKMIHH*N*P 239
+FAE ++++ IP EAL CGLT + + + N P
Sbjct: 542 VFAEGLNRIGKIPDEALGCGLTKQFLMNRNNP 573
>ref|NP_680176.1| Expressed protein; protein id: At5g21105.1 [Arabidopsis thaliana]
Length = 571
Score = 208 bits (529), Expect = 7e-53
Identities = 93/150 (62%), Positives = 116/150 (77%)
Frame = -3
Query: 694 NIFQSPVFRNTTVGNGVYMFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGE 515
+I P F NTT GNG+Y+F N VDVI+QNAN L G SEIHPWHLHGHDFWVLGYG+
Sbjct: 421 DIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGD 480
Query: 514 GKFRPGVDEKSFNLTRAPLRNTAVIFPYGWTALRFKAG*PLVFGRSHCHIEPHLHMGMGV 335
GKF+PG+DEK++NL PLRNTA+++PYGWTA+RF P V+ HCHIEPHLHMGMGV
Sbjct: 481 GKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVW-FFHCHIEPHLHMGMGV 539
Query: 334 IFAEAVHKVKNIPREALTCGLTGKMIHH*N 245
+FAE ++++ +P EAL CGLT + + + N
Sbjct: 540 VFAEGLNRIGKVPDEALGCGLTKQFLMNRN 569
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 635,321,684
Number of Sequences: 1393205
Number of extensions: 14416428
Number of successful extensions: 40913
Number of sequences better than 10.0: 339
Number of HSP's better than 10.0 without gapping: 36136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 39241
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 31684559424
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
