Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC004356A_C01 KMC004356A_c01
(518 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAF63239.1|AF153828_1 alpha-amylase [Malus x domestica] 147 7e-35
ref|NP_177740.1| alpha-amylase, putative; protein id: At1g76130.... 141 5e-33
pir||C96789 protein T23E18.6 [imported] - Arabidopsis thaliana g... 141 5e-33
gb|AAA91884.1| alpha-amylase 125 3e-28
emb|CAD40677.1| OSJNBb0118P14.15 [Oryza sativa (japonica cultiva... 113 1e-24
>gb|AAF63239.1|AF153828_1 alpha-amylase [Malus x domestica]
Length = 413
Score = 147 bits (371), Expect = 7e-35
Identities = 65/81 (80%), Positives = 72/81 (88%)
Frame = -2
Query: 517 YDHFYDWGNSFRNEIVKLIDIRKRQGIHSRSPIRILEANDNLYSAIIGEKVCMKIGDGSW 338
YDHFYDWG+S ++IVKLIDIRKRQ IHSRS I ILEA NLYSA+IGEKVCMKIGDGSW
Sbjct: 333 YDHFYDWGDSIHDQIVKLIDIRKRQDIHSRSSITILEAQPNLYSAMIGEKVCMKIGDGSW 392
Query: 337 CPSGREWTLSTCGYNYAVWQK 275
CP+GREWTL+TCG+ YAVW K
Sbjct: 393 CPAGREWTLATCGHRYAVWNK 413
>ref|NP_177740.1| alpha-amylase, putative; protein id: At1g76130.1, supported by
cDNA: 119931. [Arabidopsis thaliana]
gi|21537093|gb|AAM61434.1| alpha-amylase, putative
[Arabidopsis thaliana]
Length = 413
Score = 141 bits (355), Expect = 5e-33
Identities = 58/81 (71%), Positives = 72/81 (88%)
Frame = -2
Query: 517 YDHFYDWGNSFRNEIVKLIDIRKRQGIHSRSPIRILEANDNLYSAIIGEKVCMKIGDGSW 338
YDHFYDWG+S ++IVKLIDIR+RQ IHSRS +R+L+A NLY+AI+GEK+CMK+GDGSW
Sbjct: 333 YDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAIVGEKICMKLGDGSW 392
Query: 337 CPSGREWTLSTCGYNYAVWQK 275
CPSGR+WTL+T G+ YAVW K
Sbjct: 393 CPSGRDWTLATSGHRYAVWHK 413
>pir||C96789 protein T23E18.6 [imported] - Arabidopsis thaliana
gi|6573706|gb|AAF17626.1|AC009978_2 T23E18.6
[Arabidopsis thaliana]
Length = 412
Score = 141 bits (355), Expect = 5e-33
Identities = 58/81 (71%), Positives = 72/81 (88%)
Frame = -2
Query: 517 YDHFYDWGNSFRNEIVKLIDIRKRQGIHSRSPIRILEANDNLYSAIIGEKVCMKIGDGSW 338
YDHFYDWG+S ++IVKLIDIR+RQ IHSRS +R+L+A NLY+AI+GEK+CMK+GDGSW
Sbjct: 332 YDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAIVGEKICMKLGDGSW 391
Query: 337 CPSGREWTLSTCGYNYAVWQK 275
CPSGR+WTL+T G+ YAVW K
Sbjct: 392 CPSGRDWTLATSGHRYAVWHK 412
>gb|AAA91884.1| alpha-amylase
Length = 407
Score = 125 bits (314), Expect = 3e-28
Identities = 56/81 (69%), Positives = 67/81 (82%)
Frame = -2
Query: 517 YDHFYDWGNSFRNEIVKLIDIRKRQGIHSRSPIRILEANDNLYSAIIGEKVCMKIGDGSW 338
+DHFY+W NS ++IVKLI IR+ QGIHSRS IRILEA NLY+A I EKV +KIGDGSW
Sbjct: 327 FDHFYEWDNSMHDQIVKLIAIRRNQGIHSRSSIRILEAQPNLYAATIDEKVSVKIGDGSW 386
Query: 337 CPSGREWTLSTCGYNYAVWQK 275
P+G+EWTL+T G+ YAVWQK
Sbjct: 387 SPAGKEWTLATSGHRYAVWQK 407
>emb|CAD40677.1| OSJNBb0118P14.15 [Oryza sativa (japonica cultivar-group)]
Length = 412
Score = 113 bits (283), Expect = 1e-24
Identities = 51/81 (62%), Positives = 62/81 (75%)
Frame = -2
Query: 517 YDHFYDWGNSFRNEIVKLIDIRKRQGIHSRSPIRILEANDNLYSAIIGEKVCMKIGDGSW 338
YDHFY +SF I KL++IRK Q IHSRS ++ILEA+ +LYSAI+ +K+CMKIGDGSW
Sbjct: 332 YDHFYGKDDSFHGGIAKLMEIRKCQDIHSRSAVKILEASSDLYSAIVDDKLCMKIGDGSW 391
Query: 337 CPSGREWTLSTCGYNYAVWQK 275
CPSG EW L+ G YAVW K
Sbjct: 392 CPSGPEWKLAASGDRYAVWHK 412
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 416,400,790
Number of Sequences: 1393205
Number of extensions: 8647981
Number of successful extensions: 20456
Number of sequences better than 10.0: 68
Number of HSP's better than 10.0 without gapping: 20049
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 20423
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 16442828304
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
