Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC004311A_C02 KMC004311A_c02
(706 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_176040.1| 20S proteasome beta subunit G1 (PBG1); protein ... 259 2e-74
pir||T51986 C 3.4.25.1 proteasome endopeptidase complex () chain... 259 2e-74
dbj|BAA96839.1| beta 7 subunit of 20S proteasome [Oryza sativa (... 232 1e-66
emb|CAC43328.1| putative beta7 proteasome subunit [Nicotiana tab... 213 2e-54
emb|CAA74030.1| multicatalytic endopeptidase complex, proteasome... 147 4e-41
>ref|NP_176040.1| 20S proteasome beta subunit G1 (PBG1); protein id: At1g56450.1,
supported by cDNA: gi_14190404, supported by cDNA:
gi_15010589, supported by cDNA: gi_15450550, supported
by cDNA: gi_3421122 [Arabidopsis thaliana]
gi|25290070|pir||D96606 20S proteasome beta subunit
(PBG1) [imported] - Arabidopsis thaliana
gi|12323023|gb|AAG51500.1|AC058785_3 20S proteasome beta
subunit (PBG1) [Arabidopsis thaliana]
gi|14190405|gb|AAK55683.1|AF378880_1 At1g56450/F13N6_3
[Arabidopsis thaliana] gi|15010590|gb|AAK73954.1|
At1g56450/F13N6_3 [Arabidopsis thaliana]
gi|15450551|gb|AAK96453.1| At1g56450/F13N6_3
[Arabidopsis thaliana]
Length = 246
Score = 259 bits (661), Expect(2) = 2e-74
Identities = 119/141 (84%), Positives = 134/141 (94%)
Frame = -2
Query: 705 KHSLLGASGEISDFQEILRYLDELILNDNMWDDGNSLGPKEVHNYLTRMMYNRRNKFNPL 526
KHSLLGASGEISDFQEILRYLDEL LNDNMWDDGNSLGPKE+HNYLTR+MYNRRNKFNPL
Sbjct: 70 KHSLLGASGEISDFQEILRYLDELTLNDNMWDDGNSLGPKEIHNYLTRVMYNRRNKFNPL 129
Query: 525 WNSLVLGGVKNGQKYLGTVNMIGMSFEDNHVATGLGNHLARPILRDEWHENLTFEEAVRL 346
WN+LVLGGVKNG+ YLG V+MIG+SFED+HVATG GNHLARPILRDEWH +L+FE+ V+L
Sbjct: 130 WNTLVLGGVKNGKSYLGMVSMIGVSFEDDHVATGFGNHLARPILRDEWHADLSFEDGVKL 189
Query: 345 LEKCMRVLLYRDRSAVYQIQV 283
LEKCMRVLLYRDRSA+ ++Q+
Sbjct: 190 LEKCMRVLLYRDRSAINKLQI 210
Score = 42.7 bits (99), Expect(2) = 2e-74
Identities = 18/28 (64%), Positives = 23/28 (81%)
Frame = -3
Query: 188 MTISKIIEEGSTVYAPFSLKTYWDFSAF 105
+ I+KI EEG TV P+SLKTYW+FS+F
Sbjct: 208 LQIAKITEEGVTVSQPYSLKTYWEFSSF 235
>pir||T51986 C 3.4.25.1 proteasome endopeptidase complex () chain PBG1
[imported] - Arabidopsis thaliana
gi|3421123|gb|AAC32074.1| 20S proteasome beta subunit
PBG1 [Arabidopsis thaliana]
Length = 246
Score = 259 bits (661), Expect(2) = 2e-74
Identities = 119/141 (84%), Positives = 134/141 (94%)
Frame = -2
Query: 705 KHSLLGASGEISDFQEILRYLDELILNDNMWDDGNSLGPKEVHNYLTRMMYNRRNKFNPL 526
KHSLLGASGEISDFQEILRYLDEL LNDNMWDDGNSLGPKE+HNYLTR+MYNRRNKFNPL
Sbjct: 70 KHSLLGASGEISDFQEILRYLDELTLNDNMWDDGNSLGPKEIHNYLTRVMYNRRNKFNPL 129
Query: 525 WNSLVLGGVKNGQKYLGTVNMIGMSFEDNHVATGLGNHLARPILRDEWHENLTFEEAVRL 346
WN+LVLGGVKNG+ YLG V+MIG+SFED+HVATG GNHLARPILRDEWH +L+FE+ V+L
Sbjct: 130 WNTLVLGGVKNGKSYLGMVSMIGVSFEDDHVATGFGNHLARPILRDEWHADLSFEDGVKL 189
Query: 345 LEKCMRVLLYRDRSAVYQIQV 283
LEKCMRVLLYRDRSA+ ++Q+
Sbjct: 190 LEKCMRVLLYRDRSAINKLQI 210
Score = 42.7 bits (99), Expect(2) = 2e-74
Identities = 18/28 (64%), Positives = 23/28 (81%)
Frame = -3
Query: 188 MTISKIIEEGSTVYAPFSLKTYWDFSAF 105
+ I+KI EEG TV P+SLKTYW+FS+F
Sbjct: 208 LQIAKITEEGVTVSQPYSLKTYWEFSSF 235
>dbj|BAA96839.1| beta 7 subunit of 20S proteasome [Oryza sativa (japonica
cultivar-group)]
Length = 256
Score = 232 bits (592), Expect(2) = 1e-66
Identities = 108/145 (74%), Positives = 128/145 (87%), Gaps = 4/145 (2%)
Frame = -2
Query: 705 KHSLLGASGEISDFQEILRYLDELILNDNMWDDGNSLGPKEVHNYLTRMMYNRRNKFNPL 526
KHSL+GASGE SDFQEILRYLDEL L+D+MWDDGNSLGPKEVH+YLTR+MYNRRNKF+PL
Sbjct: 76 KHSLIGASGEFSDFQEILRYLDELTLSDHMWDDGNSLGPKEVHSYLTRVMYNRRNKFDPL 135
Query: 525 WNSLVLGGVKNG----QKYLGTVNMIGMSFEDNHVATGLGNHLARPILRDEWHENLTFEE 358
WNSLV+GGVK G +KYLG VNMIG FE+NH+ATG GNH+A PILR EW E++TFEE
Sbjct: 136 WNSLVIGGVKKGPKGEEKYLGMVNMIGTHFEENHIATGFGNHMAIPILRTEWREDMTFEE 195
Query: 357 AVRLLEKCMRVLLYRDRSAVYQIQV 283
AV+L+EKC+ VLLYRDRS++ + Q+
Sbjct: 196 AVKLVEKCLLVLLYRDRSSINKFQI 220
Score = 43.1 bits (100), Expect(2) = 1e-66
Identities = 17/26 (65%), Positives = 22/26 (84%)
Frame = -3
Query: 182 ISKIIEEGSTVYAPFSLKTYWDFSAF 105
I+KI EG+T+Y P+SLKTYW F+AF
Sbjct: 220 IAKITTEGATIYPPYSLKTYWGFAAF 245
>emb|CAC43328.1| putative beta7 proteasome subunit [Nicotiana tabacum]
Length = 129
Score = 213 bits (542), Expect = 2e-54
Identities = 100/109 (91%), Positives = 104/109 (94%)
Frame = -2
Query: 705 KHSLLGASGEISDFQEILRYLDELILNDNMWDDGNSLGPKEVHNYLTRMMYNRRNKFNPL 526
KHSLLGASGEISDFQEI R LDELIL DNMWDDGNSLGPKEVHNYLTRMMYNRRNKFNPL
Sbjct: 21 KHSLLGASGEISDFQEISRKLDELILYDNMWDDGNSLGPKEVHNYLTRMMYNRRNKFNPL 80
Query: 525 WNSLVLGGVKNGQKYLGTVNMIGMSFEDNHVATGLGNHLARPILRDEWH 379
WNSLVLGGVKNGQKYLGTV+MIG+ +ED+HVATG GNHLARPILRDEWH
Sbjct: 81 WNSLVLGGVKNGQKYLGTVSMIGVHYEDDHVATGFGNHLARPILRDEWH 129
>emb|CAA74030.1| multicatalytic endopeptidase complex, proteasome component, beta
subunit [Arabidopsis thaliana]
Length = 121
Score = 147 bits (372), Expect(2) = 4e-41
Identities = 66/85 (77%), Positives = 79/85 (92%)
Frame = -2
Query: 537 FNPLWNSLVLGGVKNGQKYLGTVNMIGMSFEDNHVATGLGNHLARPILRDEWHENLTFEE 358
FNPLWN+LVLGGVKNG+ YLG V+MIG+SFED+HVATG GNHLARPILRDEWH +L+FE+
Sbjct: 1 FNPLWNTLVLGGVKNGKSYLGMVSMIGVSFEDDHVATGFGNHLARPILRDEWHADLSFED 60
Query: 357 AVRLLEKCMRVLLYRDRSAVYQIQV 283
V+LLEKCMRVLLYRDRSA+ ++Q+
Sbjct: 61 GVKLLEKCMRVLLYRDRSAINKLQI 85
Score = 42.7 bits (99), Expect(2) = 4e-41
Identities = 18/28 (64%), Positives = 23/28 (81%)
Frame = -3
Query: 188 MTISKIIEEGSTVYAPFSLKTYWDFSAF 105
+ I+KI EEG TV P+SLKTYW+FS+F
Sbjct: 83 LQIAKITEEGVTVSQPYSLKTYWEFSSF 110
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 609,070,985
Number of Sequences: 1393205
Number of extensions: 13451302
Number of successful extensions: 32997
Number of sequences better than 10.0: 100
Number of HSP's better than 10.0 without gapping: 31680
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32936
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 32091529758
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
