Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC004304A_C01 KMC004304A_c01
(1023 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||T10804 tonoplast intrinsic protein, delta type - upland cot... 286 e-105
pir||T48886 aquaporin [imported] - Vernicia fordii gi|3249565|gb... 278 e-102
gb|AAG44945.1|AF290618_1 putative delta TIP [Nicotiana glauca] 277 e-101
ref|NP_188245.1| delta tonoplast integral protein (delta-TIP); p... 273 e-100
pir||T48885 water channel protein MipK [imported] - common ice p... 265 e-100
>pir||T10804 tonoplast intrinsic protein, delta type - upland cotton
gi|1439609|gb|AAB04557.1| delta-tonoplast intrinsic
protein [Gossypium hirsutum]
Length = 248
Score = 286 bits (731), Expect(3) = e-105
Identities = 143/169 (84%), Positives = 154/169 (90%)
Frame = -2
Query: 1007 SIGRLDDSFSLGSIKAYMAEFISTLLFVLAGVGSAIAYGKVTSDAALDPAGLVAIAVCHA 828
+ GR DDSFSLG++KAY+AEFISTL+FV AGVGSAIAY K+T+DAALDP GLVAIAVCH
Sbjct: 5 AFGRFDDSFSLGTVKAYLAEFISTLVFVFAGVGSAIAYNKLTTDAALDPDGLVAIAVCHG 64
Query: 827 FALFVAVSVGANISGGHVKPAVTFGLALGGQITILTGIFYWIAQLLGSIVACFLLHYVTG 648
FALFVAV++GANISGGHV PAVTFGLALGGQITILTGIFYWIAQLLGSIVACFLL VTG
Sbjct: 65 FALFVAVAIGANISGGHVNPAVTFGLALGGQITILTGIFYWIAQLLGSIVACFLLKAVTG 124
Query: 647 GLETPVHGLAAGVGAFEGVVTEIIITFGLVYTVYATAADPKKGSLGTIA 501
GL P+HGL AGVGA +GVV EIIITF LVYTVYATAADPKKGSLGTIA
Sbjct: 125 GLTVPIHGLGAGVGAIQGVVMEIIITFALVYTVYATAADPKKGSLGTIA 173
Score = 104 bits (260), Expect(3) = e-105
Identities = 48/61 (78%), Positives = 55/61 (89%)
Frame = -1
Query: 453 GQFSGGSMNPARSFGPAVVSGNFHDIWIYWVGPLIGGGLAGLIYGNVFIRSHEHAPLPSE 274
G FSGGSMNPARSFGPAV SG+F+ IWIYWVGPLIGGGL+GLIYGNVF+ S +HAPL ++
Sbjct: 189 GPFSGGSMNPARSFGPAVASGDFNGIWIYWVGPLIGGGLSGLIYGNVFMNS-DHAPLSND 247
Query: 273 F 271
F
Sbjct: 248 F 248
Score = 37.4 bits (85), Expect(3) = e-105
Identities = 17/19 (89%), Positives = 18/19 (94%)
Frame = -3
Query: 505 LQPIAIGFIVGANILAAGP 449
+ PIAIGFIVGANILAAGP
Sbjct: 172 IAPIAIGFIVGANILAAGP 190
>pir||T48886 aquaporin [imported] - Vernicia fordii gi|3249565|gb|AAC39480.1|
aquaporin [Vernicia fordii]
Length = 248
Score = 278 bits (710), Expect(3) = e-102
Identities = 141/169 (83%), Positives = 150/169 (88%)
Frame = -2
Query: 1007 SIGRLDDSFSLGSIKAYMAEFISTLLFVLAGVGSAIAYGKVTSDAALDPAGLVAIAVCHA 828
+ GR DDSFSLGS KAY+AEFISTLL+V AGVGSAIAY K+T++AALDPAGLVAIA+CH
Sbjct: 5 AFGRFDDSFSLGSFKAYLAEFISTLLYVFAGVGSAIAYNKLTANAALDPAGLVAIAICHG 64
Query: 827 FALFVAVSVGANISGGHVKPAVTFGLALGGQITILTGIFYWIAQLLGSIVACFLLHYVTG 648
FALFVAV+VGANISGGHV PAV FGLALGGQITILTGIFYWIAQLLGSIVACFLL VTG
Sbjct: 65 FALFVAVAVGANISGGHVNPAVAFGLALGGQITILTGIFYWIAQLLGSIVACFLLKLVTG 124
Query: 647 GLETPVHGLAAGVGAFEGVVTEIIITFGLVYTVYATAADPKKGSLGTIA 501
GL P H +A VGA EGVV EIIITF LVYTVYATAADPKKGSLGTIA
Sbjct: 125 GLAIPTHSVAGRVGAIEGVVMEIIITFALVYTVYATAADPKKGSLGTIA 173
Score = 103 bits (257), Expect(3) = e-102
Identities = 48/61 (78%), Positives = 54/61 (87%)
Frame = -1
Query: 453 GQFSGGSMNPARSFGPAVVSGNFHDIWIYWVGPLIGGGLAGLIYGNVFIRSHEHAPLPSE 274
G FSGGSM PARSFGPAVVSG+FHD WIYWVGPLIGGGLAGLIYGN++I S +H PL ++
Sbjct: 189 GPFSGGSMYPARSFGPAVVSGDFHDNWIYWVGPLIGGGLAGLIYGNLYI-SGDHTPLSND 247
Query: 273 F 271
F
Sbjct: 248 F 248
Score = 37.4 bits (85), Expect(3) = e-102
Identities = 17/19 (89%), Positives = 18/19 (94%)
Frame = -3
Query: 505 LQPIAIGFIVGANILAAGP 449
+ PIAIGFIVGANILAAGP
Sbjct: 172 IAPIAIGFIVGANILAAGP 190
>gb|AAG44945.1|AF290618_1 putative delta TIP [Nicotiana glauca]
Length = 248
Score = 277 bits (708), Expect(3) = e-101
Identities = 140/169 (82%), Positives = 153/169 (89%)
Frame = -2
Query: 1007 SIGRLDDSFSLGSIKAYMAEFISTLLFVLAGVGSAIAYGKVTSDAALDPAGLVAIAVCHA 828
+ GR+DDSFS+GS+KAY+AEFISTLLFV AGVGSAIAY K+T++AALDPAGLVA+AVCH
Sbjct: 5 AFGRIDDSFSVGSLKAYLAEFISTLLFVFAGVGSAIAYNKLTANAALDPAGLVAVAVCHG 64
Query: 827 FALFVAVSVGANISGGHVKPAVTFGLALGGQITILTGIFYWIAQLLGSIVACFLLHYVTG 648
FALFVAV+VGANISGGHV PAVTFGLALGGQIT+LTG+FY IAQLLGSIVAC LL VTG
Sbjct: 65 FALFVAVAVGANISGGHVNPAVTFGLALGGQITLLTGLFYIIAQLLGSIVACLLLKVVTG 124
Query: 647 GLETPVHGLAAGVGAFEGVVTEIIITFGLVYTVYATAADPKKGSLGTIA 501
GL P H +AAGVGA EGVV EIIITF LVYTVYATAADPKKGSLGTIA
Sbjct: 125 GLAVPTHNVAAGVGALEGVVMEIIITFALVYTVYATAADPKKGSLGTIA 173
Score = 100 bits (250), Expect(3) = e-101
Identities = 46/61 (75%), Positives = 52/61 (84%)
Frame = -1
Query: 453 GQFSGGSMNPARSFGPAVVSGNFHDIWIYWVGPLIGGGLAGLIYGNVFIRSHEHAPLPSE 274
G FSGGSMNPARSFGPAV SG+F + WIYW GPL+GGGLAGL Y NVF++ HEHAPL S+
Sbjct: 189 GPFSGGSMNPARSFGPAVASGDFTNNWIYWAGPLVGGGLAGLTYSNVFMQ-HEHAPLSSD 247
Query: 273 F 271
F
Sbjct: 248 F 248
Score = 37.4 bits (85), Expect(3) = e-101
Identities = 17/19 (89%), Positives = 18/19 (94%)
Frame = -3
Query: 505 LQPIAIGFIVGANILAAGP 449
+ PIAIGFIVGANILAAGP
Sbjct: 172 IAPIAIGFIVGANILAAGP 190
>ref|NP_188245.1| delta tonoplast integral protein (delta-TIP); protein id:
At3g16240.1, supported by cDNA: 19689., supported by
cDNA: gi_1145696, supported by cDNA: gi_17473871,
supported by cDNA: gi_20148586 [Arabidopsis thaliana]
gi|1145697|gb|AAC49281.1| delta tonoplast integral
protein gi|9279707|dbj|BAB01264.1| delta tonoplast
intrinsic protein [Arabidopsis thaliana]
gi|21554052|gb|AAM63133.1| delta tonoplast integral
protein delta-TIP [Arabidopsis thaliana]
Length = 250
Score = 273 bits (699), Expect(3) = e-100
Identities = 135/169 (79%), Positives = 149/169 (87%)
Frame = -2
Query: 1007 SIGRLDDSFSLGSIKAYMAEFISTLLFVLAGVGSAIAYGKVTSDAALDPAGLVAIAVCHA 828
+ G DDSFSL S++AY+AEFISTLLFV AGVGSAIAY K+TSDAALD GLVAIAVCH
Sbjct: 5 AFGSFDDSFSLASLRAYLAEFISTLLFVFAGVGSAIAYAKLTSDAALDTPGLVAIAVCHG 64
Query: 827 FALFVAVSVGANISGGHVKPAVTFGLALGGQITILTGIFYWIAQLLGSIVACFLLHYVTG 648
FALFVAV++GANISGGHV PAVTFGLA+GGQIT++TG+FYWIAQLLGS ACFLL YVTG
Sbjct: 65 FALFVAVAIGANISGGHVNPAVTFGLAVGGQITVITGVFYWIAQLLGSTAACFLLKYVTG 124
Query: 647 GLETPVHGLAAGVGAFEGVVTEIIITFGLVYTVYATAADPKKGSLGTIA 501
GL P H +AAG+G+ EGVV EIIITF LVYTVYATAADPKKGSLGTIA
Sbjct: 125 GLAVPTHSVAAGLGSIEGVVMEIIITFALVYTVYATAADPKKGSLGTIA 173
Score = 102 bits (253), Expect(3) = e-100
Identities = 46/59 (77%), Positives = 50/59 (83%)
Frame = -1
Query: 453 GQFSGGSMNPARSFGPAVVSGNFHDIWIYWVGPLIGGGLAGLIYGNVFIRSHEHAPLPS 277
G FSGGSMNPARSFGPAV +G+F W+YWVGPLIGGGLAGLIYGNVF+ S EH PL S
Sbjct: 189 GPFSGGSMNPARSFGPAVAAGDFSGHWVYWVGPLIGGGLAGLIYGNVFMGSSEHVPLAS 247
Score = 34.3 bits (77), Expect(3) = e-100
Identities = 15/19 (78%), Positives = 17/19 (88%)
Frame = -3
Query: 505 LQPIAIGFIVGANILAAGP 449
+ P+AIG IVGANILAAGP
Sbjct: 172 IAPLAIGLIVGANILAAGP 190
>pir||T48885 water channel protein MipK [imported] - common ice plant
gi|4884868|gb|AAD31848.1|AF133532_1 water channel protein
MipK [Mesembryanthemum crystallinum]
Length = 248
Score = 265 bits (677), Expect(3) = e-100
Identities = 131/169 (77%), Positives = 146/169 (85%)
Frame = -2
Query: 1007 SIGRLDDSFSLGSIKAYMAEFISTLLFVLAGVGSAIAYGKVTSDAALDPAGLVAIAVCHA 828
+ GR DDSFS SIKAY+AEFISTLLFV AGVGS IA+ K+T+DAAL P+GLVA+AVCHA
Sbjct: 5 AFGRFDDSFSWASIKAYIAEFISTLLFVFAGVGSCIAFNKLTADAALSPSGLVAVAVCHA 64
Query: 827 FALFVAVSVGANISGGHVKPAVTFGLALGGQITILTGIFYWIAQLLGSIVACFLLHYVTG 648
FALFVAVSVGANISGGHV PAVTFGLA+GGQITILTG+ YWIAQL GS+ CFLL TG
Sbjct: 65 FALFVAVSVGANISGGHVNPAVTFGLAVGGQITILTGVLYWIAQLAGSVAGCFLLKAATG 124
Query: 647 GLETPVHGLAAGVGAFEGVVTEIIITFGLVYTVYATAADPKKGSLGTIA 501
GL TP+H +A+GVGA E VV EIIITF LVYTVYATA DPK+GS+GTIA
Sbjct: 125 GLTTPIHSVASGVGAIEAVVMEIIITFALVYTVYATAVDPKRGSVGTIA 173
Score = 106 bits (265), Expect(3) = e-100
Identities = 48/61 (78%), Positives = 53/61 (86%)
Frame = -1
Query: 453 GQFSGGSMNPARSFGPAVVSGNFHDIWIYWVGPLIGGGLAGLIYGNVFIRSHEHAPLPSE 274
G FSGGSMNPARSFGPA+ SG+FHD W+YWVGPLIGGGLAGL Y NVF+ HEHAPL SE
Sbjct: 189 GPFSGGSMNPARSFGPALASGDFHDHWVYWVGPLIGGGLAGLTYSNVFMH-HEHAPLSSE 247
Query: 273 F 271
+
Sbjct: 248 Y 248
Score = 38.1 bits (87), Expect(3) = e-100
Identities = 18/26 (69%), Positives = 20/26 (76%)
Frame = -3
Query: 526 RRAHWAPLQPIAIGFIVGANILAAGP 449
+R + PIAIGFIVGANILAAGP
Sbjct: 165 KRGSVGTIAPIAIGFIVGANILAAGP 190
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 920,996,470
Number of Sequences: 1393205
Number of extensions: 22584188
Number of successful extensions: 95416
Number of sequences better than 10.0: 1174
Number of HSP's better than 10.0 without gapping: 74509
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 89048
length of database: 448,689,247
effective HSP length: 124
effective length of database: 275,931,827
effective search space used: 59601274632
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
