Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC004082A_C01 KMC004082A_c01
(627 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_180566.1| G-protein beta family; protein id: At2g30050.1,... 232 3e-60
gb|AAM65095.1| putative protein transport protein SEC13 [Arabido... 227 1e-58
ref|NP_186783.1| transport protein SEC13, putative; protein id: ... 227 1e-58
dbj|BAB83081.1| Sec13p [Oryza sativa] 223 2e-57
gb|AAM65185.1| transport protein SEC13, putative [Arabidopsis th... 220 1e-56
>ref|NP_180566.1| G-protein beta family; protein id: At2g30050.1, supported by cDNA:
36577., supported by cDNA: gi_15724343 [Arabidopsis
thaliana] gi|7446122|pir||T02480 sec13-related protein
At2g30050 - Arabidopsis thaliana
gi|3150415|gb|AAC16967.1| putative protein transport
protein SEC13 [Arabidopsis thaliana]
gi|15724344|gb|AAL06565.1|AF412112_1 At2g30050/F23F1.3
[Arabidopsis thaliana] gi|20197232|gb|AAM14986.1|
putative protein transport protein SEC13 [Arabidopsis
thaliana] gi|21700867|gb|AAM70557.1| At2g30050/F23F1.3
[Arabidopsis thaliana]
Length = 302
Score = 232 bits (592), Expect = 3e-60
Identities = 102/120 (85%), Positives = 112/120 (93%)
Frame = -3
Query: 625 GCDNTVKVWKLTHGNWKMDCFPALHMHTDWVRDVAWAPDLGLPKSTIASASQDGKVIIWT 446
GCDNTVKVWKL +G+WKMDCFPAL HTDWVRDVAWAP+LGLPKSTIAS SQDGKVIIWT
Sbjct: 183 GCDNTVKVWKLANGSWKMDCFPALQKHTDWVRDVAWAPNLGLPKSTIASGSQDGKVIIWT 242
Query: 445 VG*EGDQWEGKILNDFKTPVWRVSWSLTGNILAVADGNNNVTLWKEAVDGEWQQVTTVDP 266
VG EG+QWEGK+L DF TPVWRVSWSLTGN+LAV+DGNNNVT+WKEAVDGEW+QVT V+P
Sbjct: 243 VGKEGEQWEGKVLKDFMTPVWRVSWSLTGNLLAVSDGNNNVTVWKEAVDGEWEQVTAVEP 302
Score = 47.8 bits (112), Expect = 1e-04
Identities = 33/113 (29%), Positives = 54/113 (47%), Gaps = 3/113 (2%)
Frame = -3
Query: 619 DNTVKVWKLTHGNWKMDCFPALHMHTDWVRDVAWA-PDLGLPKSTIASASQDGKVIIWTV 443
D T+K+ +++ L H V +VAWA P G S +AS S DG+VI+W
Sbjct: 32 DCTIKITGVSNNGGSQQ-LATLTGHRGPVWEVAWAHPKYG---SILASCSYDGQVILWKE 87
Query: 442 G*EGDQWEGKILNDFKTPVWRVSWSL--TGNILAVADGNNNVTLWKEAVDGEW 290
G + + + D K+ V ++W+ G LA + N++++ DG W
Sbjct: 88 GNQNQWTQDHVFTDHKSSVNSIAWAPHDIGLSLACGSSDGNISVFTARADGGW 140
Score = 43.9 bits (102), Expect = 0.002
Identities = 30/90 (33%), Positives = 40/90 (44%), Gaps = 2/90 (2%)
Frame = -3
Query: 547 HTDWVRDVAWAPDLGLPKSTIASASQDGKVIIWTVG*EGDQWEGKILNDFKTPVWRVSWS 368
H D V DV + IA+AS D + I V G + L + PVW V+W+
Sbjct: 10 HEDIVHDV----QMDYYGKRIATASSDCTIKITGVSNNGGSQQLATLTGHRGPVWEVAWA 65
Query: 367 --LTGNILAVADGNNNVTLWKEAVDGEWQQ 284
G+ILA + V LWKE +W Q
Sbjct: 66 HPKYGSILASCSYDGQVILWKEGNQNQWTQ 95
Score = 40.4 bits (93), Expect = 0.019
Identities = 39/130 (30%), Positives = 58/130 (44%), Gaps = 19/130 (14%)
Frame = -3
Query: 619 DNTVKVWKLTHGN-WKMDCFPALHMHTDW---VRDVAWAP-DLGLPKSTIASASQDGKVI 455
D V +WK + N W D H+ TD V +AWAP D+GL ++A S DG +
Sbjct: 79 DGQVILWKEGNQNQWTQD-----HVFTDHKSSVNSIAWAPHDIGL---SLACGSSDGNIS 130
Query: 454 IWTVG*EGDQWEGKILNDFKTPVWRVSWS------------LTGNILAVADG--NNNVTL 317
++T +G +I V VSW+ L + +A G +N V +
Sbjct: 131 VFTARADGGWDTSRIDQAHPVGVTSVSWAPATAPGALVSSGLLDPVYKLASGGCDNTVKV 190
Query: 316 WKEAVDGEWQ 287
WK A +G W+
Sbjct: 191 WKLA-NGSWK 199
>gb|AAM65095.1| putative protein transport protein SEC13 [Arabidopsis thaliana]
Length = 302
Score = 227 bits (578), Expect = 1e-58
Identities = 101/120 (84%), Positives = 111/120 (92%)
Frame = -3
Query: 625 GCDNTVKVWKLTHGNWKMDCFPALHMHTDWVRDVAWAPDLGLPKSTIASASQDGKVIIWT 446
GCDNTVKVWKL +G+WKMDCFPAL HTDWVRDVAWAP+LGLPKSTIAS SQDGKVIIWT
Sbjct: 183 GCDNTVKVWKLANGSWKMDCFPALQKHTDWVRDVAWAPNLGLPKSTIASGSQDGKVIIWT 242
Query: 445 VG*EGDQWEGKILNDFKTPVWRVSWSLTGNILAVADGNNNVTLWKEAVDGEWQQVTTVDP 266
VG EG+QWEGK+L DF TPVWRVS SLTGN+LAV+DGNNNVT+WKEAVDGEW+QVT V+P
Sbjct: 243 VGKEGEQWEGKVLKDFMTPVWRVSSSLTGNLLAVSDGNNNVTVWKEAVDGEWEQVTAVEP 302
Score = 47.8 bits (112), Expect = 1e-04
Identities = 33/113 (29%), Positives = 54/113 (47%), Gaps = 3/113 (2%)
Frame = -3
Query: 619 DNTVKVWKLTHGNWKMDCFPALHMHTDWVRDVAWA-PDLGLPKSTIASASQDGKVIIWTV 443
D T+K+ +++ L H V +VAWA P G S +AS S DG+VI+W
Sbjct: 32 DCTIKITGVSNNGGSQQ-LATLTGHRGPVWEVAWAHPKYG---SILASCSYDGQVILWKE 87
Query: 442 G*EGDQWEGKILNDFKTPVWRVSWSL--TGNILAVADGNNNVTLWKEAVDGEW 290
G + + + D K+ V ++W+ G LA + N++++ DG W
Sbjct: 88 GNQNQWTQDHVFTDHKSSVNSIAWAPHDIGLSLACGSSDGNISVFTARADGGW 140
Score = 43.9 bits (102), Expect = 0.002
Identities = 30/90 (33%), Positives = 40/90 (44%), Gaps = 2/90 (2%)
Frame = -3
Query: 547 HTDWVRDVAWAPDLGLPKSTIASASQDGKVIIWTVG*EGDQWEGKILNDFKTPVWRVSWS 368
H D V DV + IA+AS D + I V G + L + PVW V+W+
Sbjct: 10 HEDIVHDV----QMDYYGKRIATASSDCTIKITGVSNNGGSQQLATLTGHRGPVWEVAWA 65
Query: 367 --LTGNILAVADGNNNVTLWKEAVDGEWQQ 284
G+ILA + V LWKE +W Q
Sbjct: 66 HPKYGSILASCSYDGQVILWKEGNQNQWTQ 95
Score = 40.4 bits (93), Expect = 0.019
Identities = 39/130 (30%), Positives = 58/130 (44%), Gaps = 19/130 (14%)
Frame = -3
Query: 619 DNTVKVWKLTHGN-WKMDCFPALHMHTDW---VRDVAWAP-DLGLPKSTIASASQDGKVI 455
D V +WK + N W D H+ TD V +AWAP D+GL ++A S DG +
Sbjct: 79 DGQVILWKEGNQNQWTQD-----HVFTDHKSSVNSIAWAPHDIGL---SLACGSSDGNIS 130
Query: 454 IWTVG*EGDQWEGKILNDFKTPVWRVSWS------------LTGNILAVADG--NNNVTL 317
++T +G +I V VSW+ L + +A G +N V +
Sbjct: 131 VFTARADGGWDTSRIDQAHPVGVTSVSWAPATAPGALVSSGLLDPVYKLASGGCDNTVKV 190
Query: 316 WKEAVDGEWQ 287
WK A +G W+
Sbjct: 191 WKLA-NGSWK 199
>ref|NP_186783.1| transport protein SEC13, putative; protein id: At3g01340.1,
supported by cDNA: 37331., supported by cDNA:
gi_13877998, supported by cDNA: gi_17104728 [Arabidopsis
thaliana] gi|6094550|gb|AAF03492.1|AC010676_2 putative
protein transport protein SEC13 [Arabidopsis thaliana]
gi|13877999|gb|AAK44077.1|AF370262_1 putative transport
protein SEC13 [Arabidopsis thaliana]
gi|17104729|gb|AAL34253.1| putative transport protein
SEC13 [Arabidopsis thaliana]
Length = 302
Score = 227 bits (578), Expect = 1e-58
Identities = 97/120 (80%), Positives = 113/120 (93%)
Frame = -3
Query: 625 GCDNTVKVWKLTHGNWKMDCFPALHMHTDWVRDVAWAPDLGLPKSTIASASQDGKVIIWT 446
GCD+TVKVWK ++G+WKMDCFPAL+ HTDWVRDVAWAP+LGLPKSTIAS S+DGKVIIWT
Sbjct: 183 GCDSTVKVWKFSNGSWKMDCFPALNKHTDWVRDVAWAPNLGLPKSTIASGSEDGKVIIWT 242
Query: 445 VG*EGDQWEGKILNDFKTPVWRVSWSLTGNILAVADGNNNVTLWKEAVDGEWQQVTTVDP 266
+G EG+QWEG +L DFKTPVWRVSWSLTGN+LAV+DGNNNVT+WKE+VDGEW+QVT V+P
Sbjct: 243 IGKEGEQWEGTVLKDFKTPVWRVSWSLTGNLLAVSDGNNNVTVWKESVDGEWEQVTVVEP 302
Score = 46.6 bits (109), Expect = 3e-04
Identities = 34/120 (28%), Positives = 55/120 (45%), Gaps = 3/120 (2%)
Frame = -3
Query: 619 DNTVKVWKLTHGNWKMDCFPALHMHTDWVRDVAWA-PDLGLPKSTIASASQDGKVIIWTV 443
D T+K+ +++ L H V VAWA P G S +AS S DG++I+W
Sbjct: 32 DCTIKITGVSNSGGSQH-LATLTGHRGPVWQVAWAHPKFG---SLLASCSYDGQIILWKE 87
Query: 442 G*EGDQWEGKILNDFKTPVWRVSWS--LTGNILAVADGNNNVTLWKEAVDGEWQQVTTVD 269
G + + + D K V ++W+ G LA + N++++ DG W T +D
Sbjct: 88 GNQNQWTQAHVFTDHKVSVNSIAWAPHELGLSLACGASDGNISVFSARADGGW-DTTKID 146
Score = 42.7 bits (99), Expect = 0.004
Identities = 26/90 (28%), Positives = 41/90 (44%), Gaps = 2/90 (2%)
Frame = -3
Query: 547 HTDWVRDVAWAPDLGLPKSTIASASQDGKVIIWTVG*EGDQWEGKILNDFKTPVWRVSWS 368
H+D + DV + +A+AS D + I V G L + PVW+V+W+
Sbjct: 10 HSDTIHDVV----MDYYGKRVATASSDCTIKITGVSNSGGSQHLATLTGHRGPVWQVAWA 65
Query: 367 --LTGNILAVADGNNNVTLWKEAVDGEWQQ 284
G++LA + + LWKE +W Q
Sbjct: 66 HPKFGSLLASCSYDGQIILWKEGNQNQWTQ 95
>dbj|BAB83081.1| Sec13p [Oryza sativa]
Length = 305
Score = 223 bits (567), Expect = 2e-57
Identities = 98/119 (82%), Positives = 111/119 (92%)
Frame = -3
Query: 625 GCDNTVKVWKLTHGNWKMDCFPALHMHTDWVRDVAWAPDLGLPKSTIASASQDGKVIIWT 446
GCDNTVKVWKL +G+W+MDCFPAL MH DWVRDVAWAP+LGLPKSTIASASQDG V+IWT
Sbjct: 186 GCDNTVKVWKLYNGSWRMDCFPALQMHRDWVRDVAWAPNLGLPKSTIASASQDGTVVIWT 245
Query: 445 VG*EGDQWEGKILNDFKTPVWRVSWSLTGNILAVADGNNNVTLWKEAVDGEWQQVTTVD 269
EG+QWEG++L DF+TPVWR+SWSLTGNILAV+DGN+NVTLWKEAVDGEWQQVTTV+
Sbjct: 246 APKEGEQWEGRVLYDFQTPVWRLSWSLTGNILAVSDGNDNVTLWKEAVDGEWQQVTTVE 304
Score = 50.1 bits (118), Expect = 2e-05
Identities = 38/121 (31%), Positives = 60/121 (49%), Gaps = 4/121 (3%)
Frame = -3
Query: 619 DNTVKVWKLTHGNWKMDCFPALHMHTDWVRDVAWA-PDLGLPKSTIASASQDGKVIIWTV 443
DNT+K+ ++ + + L H V VAWA P G S +AS S DG+VIIW
Sbjct: 32 DNTIKIIGVSGNSHQQ--LATLSGHQGPVWQVAWAHPKYG---SLLASCSYDGRVIIWKE 86
Query: 442 G*EGDQW-EGKILNDFKTPVWRVSWS--LTGNILAVADGNNNVTLWKEAVDGEWQQVTTV 272
G + D+W + + K+ V ++W+ G LA + N++++ DG W T +
Sbjct: 87 GSKPDEWAQAHTFIEHKSSVNSIAWAPHELGLCLACGSSDGNISVFTARSDGGW-DTTRI 145
Query: 271 D 269
D
Sbjct: 146 D 146
>gb|AAM65185.1| transport protein SEC13, putative [Arabidopsis thaliana]
Length = 301
Score = 220 bits (560), Expect = 1e-56
Identities = 95/120 (79%), Positives = 112/120 (93%)
Frame = -3
Query: 625 GCDNTVKVWKLTHGNWKMDCFPALHMHTDWVRDVAWAPDLGLPKSTIASASQDGKVIIWT 446
GCD+TVKVWK ++G+WKMDCFPAL+ HTDWVRDVAWAP+LGLPKSTIAS S+DGKVIIWT
Sbjct: 183 GCDSTVKVWKFSNGSWKMDCFPALNKHTDWVRDVAWAPNLGLPKSTIASGSEDGKVIIWT 242
Query: 445 VG*EGDQWEGKILNDFKTPVWRVSWSLTGNILAVADGNNNVTLWKEAVDGEWQQVTTVDP 266
+G +G+QWEG +L DFKTPVWRVSWSLT N+LAV+DGNNNVT+WKE+VDGEW+QVT V+P
Sbjct: 243 IG-KGEQWEGTVLKDFKTPVWRVSWSLTANLLAVSDGNNNVTVWKESVDGEWEQVTVVEP 301
Score = 46.6 bits (109), Expect = 3e-04
Identities = 34/120 (28%), Positives = 55/120 (45%), Gaps = 3/120 (2%)
Frame = -3
Query: 619 DNTVKVWKLTHGNWKMDCFPALHMHTDWVRDVAWA-PDLGLPKSTIASASQDGKVIIWTV 443
D T+K+ +++ L H V VAWA P G S +AS S DG++I+W
Sbjct: 32 DCTIKITGVSNSGGSQH-LATLTGHRGPVWQVAWAHPKFG---SLLASCSYDGQIILWKE 87
Query: 442 G*EGDQWEGKILNDFKTPVWRVSWS--LTGNILAVADGNNNVTLWKEAVDGEWQQVTTVD 269
G + + + D K V ++W+ G LA + N++++ DG W T +D
Sbjct: 88 GNQNQWTQAHVFTDHKVSVNSIAWAPHELGLSLACGASDGNISVFSARADGGW-DTTKID 146
Score = 42.7 bits (99), Expect = 0.004
Identities = 26/90 (28%), Positives = 41/90 (44%), Gaps = 2/90 (2%)
Frame = -3
Query: 547 HTDWVRDVAWAPDLGLPKSTIASASQDGKVIIWTVG*EGDQWEGKILNDFKTPVWRVSWS 368
H+D + DV + +A+AS D + I V G L + PVW+V+W+
Sbjct: 10 HSDTIHDVV----MDYYGKRVATASSDCTIKITGVSNSGGSQHLATLTGHRGPVWQVAWA 65
Query: 367 --LTGNILAVADGNNNVTLWKEAVDGEWQQ 284
G++LA + + LWKE +W Q
Sbjct: 66 HPKFGSLLASCSYDGQIILWKEGNQNQWTQ 95
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 575,604,828
Number of Sequences: 1393205
Number of extensions: 12983835
Number of successful extensions: 35494
Number of sequences better than 10.0: 561
Number of HSP's better than 10.0 without gapping: 32519
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 35044
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 25586195130
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
