Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003977A_C01 KMC003977A_c01
(657 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAM66071.1| unknown [Arabidopsis thaliana] 139 5e-43
ref|NP_680396.1| putative protein; protein id: At5g45410.2, supp... 136 3e-42
gb|AAK93585.2| unknown protein [Arabidopsis thaliana] 136 3e-40
ref|NP_194233.1| putative protein; protein id: At4g25030.1, supp... 124 2e-35
gb|AAG46152.1|AC018727_4 unknown protein [Oryza sativa] 112 8e-34
>gb|AAM66071.1| unknown [Arabidopsis thaliana]
Length = 343
Score = 139 bits (350), Expect(3) = 5e-43
Identities = 72/132 (54%), Positives = 89/132 (66%)
Frame = +2
Query: 257 AFKLFHGKDGVVPLSERSDFYDGSAAADSVPVFNSFTW*SAATISLSALGVGGPFGFWNF 436
AFKLFHGKDG+VPLS +D + A + FN ATISLSA G GGPFGF F
Sbjct: 56 AFKLFHGKDGIVPLSGFADDSEDEAGRRAPLQFNPLAG-KVATISLSAFGPGGPFGFGPF 114
Query: 437 SEKWKKQKNSESSSKKEHSSQKGDVSKHEALGNEWLKSGTCPMAKSYRAVSRVLPLVAIA 616
SEKWKKQ+ SK + Q GD SKHEA+G+EWLK+G CP+AKS+RA S+V+PL++ A
Sbjct: 115 SEKWKKQQKKPKPSKNQ---QSGDSSKHEAVGDEWLKTGNCPIAKSFRAASKVMPLISKA 171
Query: 617 FKPAAGVNLRCP 652
+G+ RCP
Sbjct: 172 LTLPSGMKYRCP 183
Score = 46.2 bits (108), Expect(3) = 5e-43
Identities = 24/41 (58%), Positives = 27/41 (65%), Gaps = 1/41 (2%)
Frame = +3
Query: 102 ECPFDA-SDIQRCPFLRNINEPANFCFSLGKVSMPVARG*G 221
ECPF A S IQ+CPFLRNIN+P N FS +PV G G
Sbjct: 4 ECPFAAESIIQKCPFLRNINKPTNLSFSSLSFPIPVQGGKG 44
Score = 32.0 bits (71), Expect(3) = 5e-43
Identities = 12/15 (80%), Positives = 13/15 (86%)
Frame = +1
Query: 211 GAKGPIFEDGPSFDT 255
G KGPIFEDGP FD+
Sbjct: 41 GGKGPIFEDGPGFDS 55
>ref|NP_680396.1| putative protein; protein id: At5g45410.2, supported by cDNA: 767.
[Arabidopsis thaliana] gi|9758731|dbj|BAB09169.1|
gene_id:MFC19.8~pir||T05524~similar to unknown protein
[Arabidopsis thaliana] gi|27754261|gb|AAO22584.1|
unknown protein [Arabidopsis thaliana]
Length = 342
Score = 136 bits (343), Expect(3) = 3e-42
Identities = 72/132 (54%), Positives = 89/132 (66%)
Frame = +2
Query: 257 AFKLFHGKDGVVPLSERSDFYDGSAAADSVPVFNSFTW*SAATISLSALGVGGPFGFWNF 436
AFKLFHGKDG+VPLS +D + A ++ FN ATISLSA G GGPFGF F
Sbjct: 56 AFKLFHGKDGIVPLSGFADDSEDEAGRRALQ-FNPLAG-KVATISLSAFGPGGPFGFGPF 113
Query: 437 SEKWKKQKNSESSSKKEHSSQKGDVSKHEALGNEWLKSGTCPMAKSYRAVSRVLPLVAIA 616
SEKWKKQ+ SK + Q GD SKHEA+G+EWLK+G CP+AKS+RA S+V+PL++ A
Sbjct: 114 SEKWKKQQKKPKPSKNQ---QSGDSSKHEAVGDEWLKTGNCPIAKSFRAASKVMPLISKA 170
Query: 617 FKPAAGVNLRCP 652
G+ RCP
Sbjct: 171 LTLPPGMKYRCP 182
Score = 46.2 bits (108), Expect(3) = 3e-42
Identities = 24/41 (58%), Positives = 27/41 (65%), Gaps = 1/41 (2%)
Frame = +3
Query: 102 ECPFDA-SDIQRCPFLRNINEPANFCFSLGKVSMPVARG*G 221
ECPF A S IQ+CPFLRNIN+P N FS +PV G G
Sbjct: 4 ECPFAAESIIQKCPFLRNINKPTNLSFSSLSFPIPVQGGKG 44
Score = 32.0 bits (71), Expect(3) = 3e-42
Identities = 12/15 (80%), Positives = 13/15 (86%)
Frame = +1
Query: 211 GAKGPIFEDGPSFDT 255
G KGPIFEDGP FD+
Sbjct: 41 GGKGPIFEDGPGFDS 55
>gb|AAK93585.2| unknown protein [Arabidopsis thaliana]
Length = 331
Score = 136 bits (343), Expect(3) = 3e-40
Identities = 72/132 (54%), Positives = 89/132 (66%)
Frame = +2
Query: 257 AFKLFHGKDGVVPLSERSDFYDGSAAADSVPVFNSFTW*SAATISLSALGVGGPFGFWNF 436
AFKLFHGKDG+VPLS +D + A ++ FN ATISLSA G GGPFGF F
Sbjct: 45 AFKLFHGKDGIVPLSGFADDSEDEAGRRALQ-FNPLAG-KVATISLSAFGPGGPFGFGPF 102
Query: 437 SEKWKKQKNSESSSKKEHSSQKGDVSKHEALGNEWLKSGTCPMAKSYRAVSRVLPLVAIA 616
SEKWKKQ+ SK + Q GD SKHEA+G+EWLK+G CP+AKS+RA S+V+PL++ A
Sbjct: 103 SEKWKKQQKKPKPSKNQ---QSGDSSKHEAVGDEWLKTGNCPIAKSFRAASKVMPLISKA 159
Query: 617 FKPAAGVNLRCP 652
G+ RCP
Sbjct: 160 LTLPPGMKYRCP 171
Score = 39.3 bits (90), Expect(3) = 3e-40
Identities = 18/32 (56%), Positives = 21/32 (65%)
Frame = +3
Query: 126 IQRCPFLRNINEPANFCFSLGKVSMPVARG*G 221
IQ+CPFLRNIN+P N FS +PV G G
Sbjct: 2 IQKCPFLRNINKPTNLSFSSLSFPIPVQGGKG 33
Score = 32.0 bits (71), Expect(3) = 3e-40
Identities = 12/15 (80%), Positives = 13/15 (86%)
Frame = +1
Query: 211 GAKGPIFEDGPSFDT 255
G KGPIFEDGP FD+
Sbjct: 30 GGKGPIFEDGPGFDS 44
>ref|NP_194233.1| putative protein; protein id: At4g25030.1, supported by cDNA:
16463. [Arabidopsis thaliana] gi|7485448|pir||T05524
hypothetical protein F13M23.170 - Arabidopsis thaliana
gi|4455246|emb|CAB36745.1| putative protein [Arabidopsis
thaliana] gi|7269353|emb|CAB79412.1| putative protein
[Arabidopsis thaliana] gi|21553767|gb|AAM62860.1|
unknown [Arabidopsis thaliana]
Length = 344
Score = 124 bits (310), Expect(3) = 2e-35
Identities = 68/132 (51%), Positives = 86/132 (64%)
Frame = +2
Query: 257 AFKLFHGKDGVVPLSERSDFYDGSAAADSVPVFNSFTW*SAATISLSALGVGGPFGFWNF 436
AF+LFHG+DGVVPLS+ + + A VPVF+ AATISLS+ G GGPFGF F
Sbjct: 60 AFRLFHGQDGVVPLSDTAR----TEAQKPVPVFHPLAA-KAATISLSSFGSGGPFGFDAF 114
Query: 437 SEKWKKQKNSESSSKKEHSSQKGDVSKHEALGNEWLKSGTCPMAKSYRAVSRVLPLVAIA 616
S+ +K QK SSK + + HEA+G+EWLK+G CP+AKSYRAVS V PLVA
Sbjct: 115 SDMFKNQKKKSDSSKNKGGN-------HEAMGDEWLKTGNCPIAKSYRAVSGVAPLVAKI 167
Query: 617 FKPAAGVNLRCP 652
+P G+ +CP
Sbjct: 168 LQPPPGMKFKCP 179
Score = 36.2 bits (82), Expect(3) = 2e-35
Identities = 15/20 (75%), Positives = 16/20 (80%)
Frame = +3
Query: 123 DIQRCPFLRNINEPANFCFS 182
+I RCPFLRNINEP N FS
Sbjct: 15 NILRCPFLRNINEPTNLSFS 34
Score = 31.6 bits (70), Expect(3) = 2e-35
Identities = 12/13 (92%), Positives = 13/13 (99%)
Frame = +1
Query: 217 KGPIFEDGPSFDT 255
KGPIFEDGP+FDT
Sbjct: 47 KGPIFEDGPNFDT 59
>gb|AAG46152.1|AC018727_4 unknown protein [Oryza sativa]
Length = 352
Score = 112 bits (279), Expect(3) = 8e-34
Identities = 66/133 (49%), Positives = 78/133 (58%)
Frame = +2
Query: 257 AFKLFHGKDGVVPLSERSDFYDGSAAADSVPVFNSFTW*SAATISLSALGVGGPFGFWNF 436
AF++FHG+DGVVPLS S P FN AATISLSA G G F F +F
Sbjct: 67 AFRVFHGQDGVVPLSHGSFERFEKPMPKPNPEFNPLAA-KAATISLSAFG--GFFSFGDF 123
Query: 437 SEKWKKQKNSESSSKKEHSSQKGDVSKHEALGNEWLKSGTCPMAKSYRAVSRVLPLVAIA 616
S K + +KNS G + HEAL NEWL+ G CP+AKSYRA+S V+PLVA
Sbjct: 124 SNK-RNKKNSNQKKPNNLPQNGGQPNNHEALSNEWLEMGQCPLAKSYRALSGVVPLVAKM 182
Query: 617 FKPAAGVNLRCPP 655
P AG+ LRCPP
Sbjct: 183 MTPPAGMKLRCPP 195
Score = 43.1 bits (100), Expect(3) = 8e-34
Identities = 30/55 (54%), Positives = 34/55 (61%), Gaps = 4/55 (7%)
Frame = +3
Query: 63 MDFFFKGMSGDGSECPFDAS---DIQRCPFLRNINEPANFCFSLGKVSMPV-ARG 215
MD FF+ S D C D S I+RCPFLRNINEP +F FS V+ PV ARG
Sbjct: 1 MDPFFRRASSDPL-CLEDNSVQHGIERCPFLRNINEPTSFSFS--SVNFPVPARG 52
Score = 31.2 bits (69), Expect(3) = 8e-34
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = +1
Query: 211 GAKGPIFEDGPSFD 252
G KGPIFEDGP+FD
Sbjct: 52 GDKGPIFEDGPNFD 65
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 599,313,885
Number of Sequences: 1393205
Number of extensions: 13287994
Number of successful extensions: 35896
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 34242
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 35822
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 28006887348
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
