Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003912A_C02 KMC003912A_c02
(526 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAC43638.1| unknown protein [Arabidopsis thaliana] gi|290288... 147 3e-48
ref|NP_173466.1| hypothetical protein; protein id: At1g20410.1 [... 90 2e-31
ref|NP_653310.1| hypothetical protein FLJ32312 [Homo sapiens] gi... 85 1e-23
gb|AAF79615.1|AC027665_16 F5M15.23 [Arabidopsis thaliana] 107 7e-23
dbj|BAC26650.1| unnamed protein product [Mus musculus] 82 8e-23
>dbj|BAC43638.1| unknown protein [Arabidopsis thaliana] gi|29028890|gb|AAO64824.1|
At1g20410 [Arabidopsis thaliana]
Length = 504
Score = 147 bits (372), Expect(2) = 3e-48
Identities = 66/91 (72%), Positives = 79/91 (86%)
Frame = -1
Query: 526 WELMREGEAEKQTPYPALVWISRPLKDEDFQCVSSLNDLKVLQRTPIRVLHRRSPLEREK 347
W +MREGEAEKQ Y ALVWISRPL+++DF +S L +LK+LQ+TP+RVLHRRSPL+REK
Sbjct: 378 WAMMREGEAEKQKQYVALVWISRPLEEKDFNSISCLKELKILQKTPVRVLHRRSPLDREK 437
Query: 346 IIHWMKMETVAGSSQYFLLHLCTQAGTYIQE 254
IIHWMK+E + G S YFLLHLCTQAGTYI+E
Sbjct: 438 IIHWMKVEKIKGHSHYFLLHLCTQAGTYIKE 468
Score = 65.9 bits (159), Expect(2) = 3e-48
Identities = 31/39 (79%), Positives = 33/39 (84%)
Frame = -2
Query: 270 GPTSKNFVHGDLGRTHPSLGSILGCRAEILQLDVTDIKM 154
G K FVHGDLGRT PS GSILGCRAEI+QLDVTD+KM
Sbjct: 463 GTYIKEFVHGDLGRTTPSKGSILGCRAEIIQLDVTDVKM 501
>ref|NP_173466.1| hypothetical protein; protein id: At1g20410.1 [Arabidopsis
thaliana]
Length = 432
Score = 89.7 bits (221), Expect(2) = 2e-31
Identities = 47/91 (51%), Positives = 56/91 (60%)
Frame = -1
Query: 526 WELMREGEAEKQTPYPALVWISRPLKDEDFQCVSSLNDLKVLQRTPIRVLHRRSPLEREK 347
W +MREGEAEKQ Y ALVWISRPL+++DF +S L +L V R
Sbjct: 322 WAMMREGEAEKQKQYVALVWISRPLEEKDFNSISCLKELSVFVVPIFR------------ 369
Query: 346 IIHWMKMETVAGSSQYFLLHLCTQAGTYIQE 254
MK+E + G S YFLLHLCTQAGTYI+E
Sbjct: 370 ----MKVEKIKGHSHYFLLHLCTQAGTYIKE 396
Score = 67.4 bits (163), Expect(2) = 2e-31
Identities = 31/39 (79%), Positives = 34/39 (86%)
Frame = -2
Query: 270 GPTSKNFVHGDLGRTHPSLGSILGCRAEILQLDVTDIKM 154
G K FVHGDLGRT PS+GSILGCRAEI+QLDVTD+KM
Sbjct: 391 GTYIKEFVHGDLGRTTPSMGSILGCRAEIIQLDVTDVKM 429
>ref|NP_653310.1| hypothetical protein FLJ32312 [Homo sapiens]
gi|16552394|dbj|BAB71300.1| unnamed protein product
[Homo sapiens]
Length = 529
Score = 85.1 bits (209), Expect(2) = 1e-23
Identities = 40/88 (45%), Positives = 61/88 (68%)
Frame = -1
Query: 517 MREGEAEKQTPYPALVWISRPLKDEDFQCVSSLNDLKVLQRTPIRVLHRRSPLEREKIIH 338
M+EGE EK Y AL+W ++ ++ +D + ++ + DLK+ Q+TP+RVLHRR R ++IH
Sbjct: 403 MKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIH 462
Query: 337 WMKMETVAGSSQYFLLHLCTQAGTYIQE 254
+M+ + V +F LHL TQAGTYI+E
Sbjct: 463 FMETQYV--DEHHFRLHLKTQAGTYIKE 488
Score = 45.8 bits (107), Expect(2) = 1e-23
Identities = 19/40 (47%), Positives = 28/40 (69%)
Frame = -2
Query: 270 GPTSKNFVHGDLGRTHPSLGSILGCRAEILQLDVTDIKME 151
G K FVHGD GRT P++GS++ A+IL+LDV + ++
Sbjct: 483 GTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVD 522
>gb|AAF79615.1|AC027665_16 F5M15.23 [Arabidopsis thaliana]
Length = 682
Score = 107 bits (268), Expect = 7e-23
Identities = 56/104 (53%), Positives = 68/104 (64%), Gaps = 13/104 (12%)
Frame = -1
Query: 526 WELMREGEAEKQTPYPALVWISRPLKDEDFQCVSSLNDLKVLQRTPIRVLHRRSPLEREK 347
W +MREGEAEKQ Y ALVWISRPL+++DF +S L +LK+LQ+TP+RVLHRRSPL+REK
Sbjct: 552 WAMMREGEAEKQKQYVALVWISRPLEEKDFNSISCLKELKILQKTPVRVLHRRSPLDREK 611
Query: 346 IIHWMKMETVAGSSQYFLLHL-------------CTQAGTYIQE 254
IIHW+ L HL QAGTYI+E
Sbjct: 612 IIHWLLQ---------LLTHLIIILERWASFKFSIMQAGTYIKE 646
Score = 33.9 bits (76), Expect = 1.2
Identities = 16/45 (35%), Positives = 23/45 (50%)
Frame = -2
Query: 294 FCIYVHRLGPTSKNFVHGDLGRTHPSLGSILGCRAEILQLDVTDI 160
F + + G K FVHGDLGRT P + + R+ + DV +
Sbjct: 633 FKFSIMQAGTYIKEFVHGDLGRTTPRMHELARVRSRYRKYDVVSV 677
>dbj|BAC26650.1| unnamed protein product [Mus musculus]
Length = 527
Score = 81.6 bits (200), Expect(2) = 8e-23
Identities = 42/88 (47%), Positives = 56/88 (62%)
Frame = -1
Query: 517 MREGEAEKQTPYPALVWISRPLKDEDFQCVSSLNDLKVLQRTPIRVLHRRSPLEREKIIH 338
M+EGE EK Y AL+W +R ++ +D + L DLK+ Q+TP+RVLHRR R + IH
Sbjct: 401 MKEGEEEKTKTYSALIWTNRAIQKKDIGFLDDLKDLKIDQKTPLRVLHRRPLAVRTRAIH 460
Query: 337 WMKMETVAGSSQYFLLHLCTQAGTYIQE 254
MK + +F LHL TQAGTYI+E
Sbjct: 461 SMKTHYL--DEHHFRLHLKTQAGTYIKE 486
Score = 46.6 bits (109), Expect(2) = 8e-23
Identities = 20/40 (50%), Positives = 28/40 (70%)
Frame = -2
Query: 270 GPTSKNFVHGDLGRTHPSLGSILGCRAEILQLDVTDIKME 151
G K FVHGD GRT P+LGS++ A+IL+LDV + ++
Sbjct: 481 GTYIKEFVHGDFGRTKPNLGSLMNVTADILELDVESVDVD 520
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 432,800,518
Number of Sequences: 1393205
Number of extensions: 9097297
Number of successful extensions: 33144
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 32661
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 33120
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 17019769648
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
