Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003844A_C01 KMC003844A_c01
(626 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAM61752.1| putative eukaryotic translation initiation factor... 157 2e-48
ref|NP_565927.1| putative eukaryotic translation initiation fact... 157 2e-48
gb|AAK29673.1|AF353096_1 protein synthesis initiation factor eIF... 144 7e-47
ref|NP_196166.1| eukaryotic translation initiation factor 2 alph... 144 8e-46
gb|AAD25944.1|AF085279_17 hypothetical EIF-2-Alpha [Arabidopsis ... 143 3e-44
>gb|AAM61752.1| putative eukaryotic translation initiation factor 2 alpha subunit,
eIF2 [Arabidopsis thaliana]
Length = 344
Score = 157 bits (397), Expect(3) = 2e-48
Identities = 75/90 (83%), Positives = 83/90 (91%)
Frame = -1
Query: 626 PLKIRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCPVNIKLVAPPLYVLTTQTLDKEQG 447
P+KIRADIE+KCFQFDGV+HIKEAM+ AEAAGN+DCPV IKLVAPPLYVLTTQTLDKEQG
Sbjct: 204 PMKIRADIELKCFQFDGVVHIKEAMKNAEAAGNEDCPVKIKLVAPPLYVLTTQTLDKEQG 263
Query: 446 ILVLNNAIVSCTESIEKHKGKLVVKEAARA 357
I +LN AI +CTE+IE HKGKLVVKE ARA
Sbjct: 264 IEILNKAIAACTETIETHKGKLVVKEGARA 293
Score = 48.9 bits (115), Expect(3) = 2e-48
Identities = 24/33 (72%), Positives = 29/33 (87%), Gaps = 1/33 (3%)
Frame = -3
Query: 300 ENEEVSGDEDS-EEEEDTGMGEVDVDNGSVITE 205
+NEE+SGDEDS +EEEDTGMGEVD+D G+ I E
Sbjct: 312 DNEEMSGDEDSGDEEEDTGMGEVDLDAGAGIIE 344
Score = 29.3 bits (64), Expect(3) = 2e-48
Identities = 12/17 (70%), Positives = 16/17 (93%)
Frame = -2
Query: 355 VSERDDKVIAEYMAKLR 305
VSERD+K++ E+MAKLR
Sbjct: 294 VSERDEKMLTEHMAKLR 310
>ref|NP_565927.1| putative eukaryotic translation initiation factor 2 alpha subunit,
eIF2; protein id: At2g40290.1, supported by cDNA:
13800., supported by cDNA: gi_19347785 [Arabidopsis
thaliana] gi|19347786|gb|AAL86344.1| putative eukaryotic
translation initiation factor 2 alpha subunit eIF2
[Arabidopsis thaliana] gi|20198018|gb|AAD25664.2|
putative eukaryotic translation initiation factor 2
alpha subunit, eIF2 [Arabidopsis thaliana]
gi|21436249|gb|AAM51263.1| putative eukaryotic
translation initiation factor 2 alpha subunit eIF2
[Arabidopsis thaliana]
Length = 344
Score = 157 bits (397), Expect(3) = 2e-48
Identities = 75/90 (83%), Positives = 83/90 (91%)
Frame = -1
Query: 626 PLKIRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCPVNIKLVAPPLYVLTTQTLDKEQG 447
P+KIRADIE+KCFQFDGV+HIKEAM+ AEAAGN+DCPV IKLVAPPLYVLTTQTLDKEQG
Sbjct: 204 PMKIRADIELKCFQFDGVVHIKEAMKNAEAAGNEDCPVKIKLVAPPLYVLTTQTLDKEQG 263
Query: 446 ILVLNNAIVSCTESIEKHKGKLVVKEAARA 357
I +LN AI +CTE+IE HKGKLVVKE ARA
Sbjct: 264 IEILNKAIAACTETIETHKGKLVVKEGARA 293
Score = 48.9 bits (115), Expect(3) = 2e-48
Identities = 24/33 (72%), Positives = 29/33 (87%), Gaps = 1/33 (3%)
Frame = -3
Query: 300 ENEEVSGDEDS-EEEEDTGMGEVDVDNGSVITE 205
+NEE+SGDEDS +EEEDTGMGEVD+D G+ I E
Sbjct: 312 DNEEMSGDEDSGDEEEDTGMGEVDLDAGAGIIE 344
Score = 29.3 bits (64), Expect(3) = 2e-48
Identities = 12/17 (70%), Positives = 16/17 (93%)
Frame = -2
Query: 355 VSERDDKVIAEYMAKLR 305
VSERD+K++ E+MAKLR
Sbjct: 294 VSERDEKMLTEHMAKLR 310
>gb|AAK29673.1|AF353096_1 protein synthesis initiation factor eIF2 alpha [Arabidopsis
thaliana]
Length = 343
Score = 144 bits (363), Expect(3) = 7e-47
Identities = 70/90 (77%), Positives = 78/90 (85%)
Frame = -1
Query: 626 PLKIRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCPVNIKLVAPPLYVLTTQTLDKEQG 447
P+KIRADIE+KCFQFDGVLHIKEAM+KAEA G DDCPV IKLVAPPLYVLTT T KE+G
Sbjct: 204 PMKIRADIELKCFQFDGVLHIKEAMKKAEAVGTDDCPVKIKLVAPPLYVLTTHTHYKEKG 263
Query: 446 ILVLNNAIVSCTESIEKHKGKLVVKEAARA 357
I+ LN AI +C +IE+HKGKLVVKE ARA
Sbjct: 264 IVTLNKAIEACITAIEEHKGKLVVKEGARA 293
Score = 53.5 bits (127), Expect(3) = 7e-47
Identities = 24/32 (75%), Positives = 28/32 (87%)
Frame = -3
Query: 300 ENEEVSGDEDSEEEEDTGMGEVDVDNGSVITE 205
+NEE+SGDE SE+EEDTGMGEVD+D GS I E
Sbjct: 312 DNEEMSGDEGSEDEEDTGMGEVDIDGGSGIIE 343
Score = 32.3 bits (72), Expect(3) = 7e-47
Identities = 14/17 (82%), Positives = 17/17 (99%)
Frame = -2
Query: 355 VSERDDKVIAEYMAKLR 305
VSERDDK++AE+MAKLR
Sbjct: 294 VSERDDKLLAEHMAKLR 310
>ref|NP_196166.1| eukaryotic translation initiation factor 2 alpha subunit-like
protein; protein id: At5g05470.1, supported by cDNA:
gi_13358180, supported by cDNA: gi_13549159 [Arabidopsis
thaliana] gi|11762124|gb|AAG40340.1|AF324988_1 AT5g05470
[Arabidopsis thaliana] gi|26450113|dbj|BAC42176.1|
putative eukaryotic translation initiation factor 2
alpha subunit [Arabidopsis thaliana]
Length = 344
Score = 144 bits (363), Expect(3) = 8e-46
Identities = 70/90 (77%), Positives = 78/90 (85%)
Frame = -1
Query: 626 PLKIRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCPVNIKLVAPPLYVLTTQTLDKEQG 447
P+KIRADIE+KCFQFDGVLHIKEAM+KAEA G DDCPV IKLVAPPLYVLTT T KE+G
Sbjct: 204 PMKIRADIELKCFQFDGVLHIKEAMKKAEAVGTDDCPVKIKLVAPPLYVLTTHTHYKEKG 263
Query: 446 ILVLNNAIVSCTESIEKHKGKLVVKEAARA 357
I+ LN AI +C +IE+HKGKLVVKE ARA
Sbjct: 264 IVTLNKAIEACITAIEEHKGKLVVKEGARA 293
Score = 50.1 bits (118), Expect(3) = 8e-46
Identities = 25/33 (75%), Positives = 28/33 (84%), Gaps = 1/33 (3%)
Frame = -3
Query: 300 ENEEVSGDEDSE-EEEDTGMGEVDVDNGSVITE 205
+NEE+SGDE SE EEEDTGMGEVD+D GS I E
Sbjct: 312 DNEEMSGDEGSEDEEEDTGMGEVDIDGGSGIIE 344
Score = 32.3 bits (72), Expect(3) = 8e-46
Identities = 14/17 (82%), Positives = 17/17 (99%)
Frame = -2
Query: 355 VSERDDKVIAEYMAKLR 305
VSERDDK++AE+MAKLR
Sbjct: 294 VSERDDKLLAEHMAKLR 310
>gb|AAD25944.1|AF085279_17 hypothetical EIF-2-Alpha [Arabidopsis thaliana]
Length = 370
Score = 143 bits (360), Expect(3) = 3e-44
Identities = 75/105 (71%), Positives = 83/105 (78%), Gaps = 15/105 (14%)
Frame = -1
Query: 626 PLKIRADIEMKCFQFDGVLHIK-EAMRKAEAAGNDDCPVNIKLVAPPLYVLTTQTLDK-- 456
P+KIRADIE+KCFQFDGV+HIK EAM+ AEAAGN+DCPV IKLVAPPLYVLTTQTLDK
Sbjct: 215 PMKIRADIELKCFQFDGVVHIKVEAMKNAEAAGNEDCPVKIKLVAPPLYVLTTQTLDKVR 274
Query: 455 ------------EQGILVLNNAIVSCTESIEKHKGKLVVKEAARA 357
EQGI +LN AI +CTE+IE HKGKLVVKE ARA
Sbjct: 275 QSSILHYDLLGLEQGIEILNKAIAACTETIETHKGKLVVKEGARA 319
Score = 48.9 bits (115), Expect(3) = 3e-44
Identities = 24/33 (72%), Positives = 29/33 (87%), Gaps = 1/33 (3%)
Frame = -3
Query: 300 ENEEVSGDEDS-EEEEDTGMGEVDVDNGSVITE 205
+NEE+SGDEDS +EEEDTGMGEVD+D G+ I E
Sbjct: 338 DNEEMSGDEDSGDEEEDTGMGEVDLDAGAGIIE 370
Score = 29.3 bits (64), Expect(3) = 3e-44
Identities = 12/17 (70%), Positives = 16/17 (93%)
Frame = -2
Query: 355 VSERDDKVIAEYMAKLR 305
VSERD+K++ E+MAKLR
Sbjct: 320 VSERDEKMLTEHMAKLR 336
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 526,926,278
Number of Sequences: 1393205
Number of extensions: 11354416
Number of successful extensions: 53237
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 39998
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 50229
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 25586195130
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
