Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003778A_C01 KMC003778A_c01
(422 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q03943|IM30_PEA Membrane-associated 30 kDa protein, chloropla... 100 3e-21
dbj|BAB90216.1| putative chloroplast membrane-associated 30 kD p... 74 4e-13
ref|NP_564846.1| expressed protein; protein id: At1g65260.1, sup... 73 7e-13
ref|NP_681074.1| ORF_ID:tlr0283~lipid transfer protein M30 homol... 46 9e-05
ref|NP_442275.1| chloroplast membrane-associated 30 kD protein [... 45 3e-04
>sp|Q03943|IM30_PEA Membrane-associated 30 kDa protein, chloroplast precursor (M30)
gi|1076532|pir||S47966 probable lipid transfer protein
M30 precursor - garden pea gi|169107|gb|AAA53545.1| IM30
Length = 323
Score = 100 bits (250), Expect = 3e-21
Identities = 53/84 (63%), Positives = 62/84 (73%), Gaps = 2/84 (2%)
Frame = +1
Query: 175 LPLPP--PALCMLKKPLTTSFLGTGTVEVLRLSGMRVVKPVRAPRRGGARMNLFDRFARV 348
LP P P+ +LKKPL T+ GT V+ L+ MR+ KPVR G RMNLFDRFARV
Sbjct: 11 LPSAPLQPSSSLLKKPLATTLFGTRPVDTLKFRVMRIAKPVRGGGAIGVRMNLFDRFARV 70
Query: 349 VKSYANAVISSFEDPEKILEQAVL 420
VKSYANA++S+FEDPEKILEQAVL
Sbjct: 71 VKSYANALVSTFEDPEKILEQAVL 94
Score = 32.3 bits (72), Expect = 1.4
Identities = 14/20 (70%), Positives = 16/20 (80%)
Frame = +3
Query: 117 MSTKLQIFTGLPSTPFQSSS 176
M+TK QIF+GLPS P Q SS
Sbjct: 1 MTTKFQIFSGLPSAPLQPSS 20
>dbj|BAB90216.1| putative chloroplast membrane-associated 30 kD protein [Oryza
sativa (japonica cultivar-group)]
gi|21952843|dbj|BAC06258.1| putative chloroplast
membrane-associated 30 kD protein [Oryza sativa
(japonica cultivar-group)]
Length = 317
Score = 73.9 bits (180), Expect = 4e-13
Identities = 49/89 (55%), Positives = 57/89 (63%)
Frame = +1
Query: 154 PRHSNLLLPLPPPALCMLKKPLTTSFLGTGTVEVLRLSGMRVVKPVRAPRRGGARMNLFD 333
P S L P PP + + L TSFL G+V LRL +R V + G R NL D
Sbjct: 6 PPTSLRLAPPPPASASFRRTALRTSFLN-GSVS-LRLIQVRQSN-VNRFKCNGIRSNLLD 62
Query: 334 RFARVVKSYANAVISSFEDPEKILEQAVL 420
RF+RVVKSYANAV+SSFEDPEKIL+QAVL
Sbjct: 63 RFSRVVKSYANAVLSSFEDPEKILDQAVL 91
>ref|NP_564846.1| expressed protein; protein id: At1g65260.1, supported by cDNA:
32284., supported by cDNA: gi_15027976 [Arabidopsis
thaliana] gi|7447853|pir||T02347 probable lipid transfer
protein T8F5.2 - Arabidopsis thaliana
gi|3335333|gb|AAC27135.1|AAC27135 Similar to chloroplast
membrane-associated 30KD protein precursor (IM30)
gb|M73744 from Pisum sativum. ESTs gb|N37557, gb|W43887
and gb|AA042479 come from this gene. [Arabidopsis
thaliana] gi|15027977|gb|AAK76519.1| unknown protein
[Arabidopsis thaliana] gi|21436055|gb|AAM51228.1|
unknown protein [Arabidopsis thaliana]
Length = 330
Score = 73.2 bits (178), Expect = 7e-13
Identities = 42/70 (60%), Positives = 51/70 (72%), Gaps = 1/70 (1%)
Frame = +1
Query: 214 PLTTSFLGTGTVEVLRLSGMRVVKPVRAPRRG-GARMNLFDRFARVVKSYANAVISSFED 390
PL TSF G + LR++ +R+ R G GA MNLF+RF+RVVKSYANA+ISSFED
Sbjct: 37 PLRTSFFGNSS-GALRVNVLRLACDNRLRCNGHGATMNLFERFSRVVKSYANALISSFED 95
Query: 391 PEKILEQAVL 420
PEKILEQ V+
Sbjct: 96 PEKILEQTVI 105
>ref|NP_681074.1| ORF_ID:tlr0283~lipid transfer protein M30 homolog
[Thermosynechococcus elongatus BP-1]
gi|22294004|dbj|BAC07836.1| ORF_ID:tlr0283~lipid
transfer protein M30 homolog [Thermosynechococcus
elongatus BP-1]
Length = 243
Score = 46.2 bits (108), Expect = 9e-05
Identities = 20/34 (58%), Positives = 28/34 (81%)
Frame = +1
Query: 319 MNLFDRFARVVKSYANAVISSFEDPEKILEQAVL 420
M LFDR +RVV+S+ NA++ + EDPEKILEQ ++
Sbjct: 1 MGLFDRVSRVVRSWLNALVGAAEDPEKILEQTLI 34
>ref|NP_442275.1| chloroplast membrane-associated 30 kD protein [Synechocystis sp.
PCC 6803] gi|3123076|sp|Q55707|Y617_SYNY3 Hypothetical
protein sll0617 gi|7447854|pir||S76499 hypothetical
protein - Synechocystis sp. (strain PCC 6803)
gi|1001614|dbj|BAA10345.1| chloroplast
membrane-associated 30 kD protein [Synechocystis sp. PCC
6803]
Length = 267
Score = 44.7 bits (104), Expect = 3e-04
Identities = 20/34 (58%), Positives = 26/34 (75%)
Frame = +1
Query: 319 MNLFDRFARVVKSYANAVISSFEDPEKILEQAVL 420
M LFDR RVV++ N ++S EDPEK+LEQAV+
Sbjct: 1 MGLFDRLGRVVRANLNDLVSKAEDPEKVLEQAVI 34
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 395,241,089
Number of Sequences: 1393205
Number of extensions: 9294341
Number of successful extensions: 48090
Number of sequences better than 10.0: 154
Number of HSP's better than 10.0 without gapping: 40228
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 46547
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 6889859208
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
