Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003774A_C01 KMC003774A_c01
(392 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9XGI7|NAS_LYCES Nicotianamine synthase (S-adenosyl-L-methion... 107 5e-23
ref|NP_172395.1| nicotianamine synthase, putative; protein id: A... 96 8e-20
emb|CAC82913.1| nicotianamine synthase [Thlaspi caerulescens] 92 2e-18
ref|NP_176038.1| nicotianamine synthase, putative; protein id: A... 91 3e-18
dbj|BAA74590.1| nicotianamine synathase [Arabidopsis thaliana] 90 6e-18
>sp|Q9XGI7|NAS_LYCES Nicotianamine synthase
(S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-
adenosyl-methionine 3-amino-3-carboxypropyltransferase)
(Chloronerva) gi|4753801|emb|CAB42052.1| nicotianamine
synthase [Lycopersicon esculentum]
Length = 317
Score = 107 bits (266), Expect = 5e-23
Identities = 57/96 (59%), Positives = 69/96 (71%), Gaps = 9/96 (9%)
Frame = -1
Query: 392 MAPGAVLVLRSAHGARAFLYPVVDPSDLQGFEVLSVFHPTDEVINSVIVARKHSVNPGI- 216
M+PGA L+LRSAHGARAFLYPV+DP DL+GFEVLSV+HPTDEVINSVI+ARK V P +
Sbjct: 221 MSPGATLMLRSAHGARAFLYPVLDPRDLRGFEVLSVYHPTDEVINSVIIARKLPV-PSVP 279
Query: 215 --------LLSSKCHELAAEGFNTLNHRNVIDELSL 132
+L SKC FN LN N+++E +L
Sbjct: 280 LLDGLGAYVLPSKCACAEIHAFNPLNKMNLVEEFAL 315
>ref|NP_172395.1| nicotianamine synthase, putative; protein id: At1g09240.1,
supported by cDNA: 37124. [Arabidopsis thaliana]
gi|27151628|sp|O80483|NAS3_ARATH Nicotianamine synthase
3
(S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-
adenosyl-methionine 3-amino-3-carboxypropyltransferase
3) (AtNAS3) gi|25372953|pir||C86225 hypothetical protein
[imported] - Arabidopsis thaliana
gi|3249099|gb|AAC24082.1| EST gb|T21244 comes from this
gene. [Arabidopsis thaliana] gi|4220616|dbj|BAA74591.1|
nicotianamine synthase [Arabidopsis thaliana]
gi|21593212|gb|AAM65161.1| putative nicotianamine
synthase [Arabidopsis thaliana]
gi|22655164|gb|AAM98172.1| putative nicotianamine
synthase [Arabidopsis thaliana]
Length = 320
Score = 96.3 bits (238), Expect = 8e-20
Identities = 42/55 (76%), Positives = 52/55 (94%)
Frame = -1
Query: 392 MAPGAVLVLRSAHGARAFLYPVVDPSDLQGFEVLSVFHPTDEVINSVIVARKHSV 228
MAPGAVL+LRSAHG RAFLYP+V+P DLQGFEVLS++HPTD+VINSV++++KH V
Sbjct: 223 MAPGAVLMLRSAHGPRAFLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISKKHPV 277
>emb|CAC82913.1| nicotianamine synthase [Thlaspi caerulescens]
Length = 321
Score = 92.0 bits (227), Expect = 2e-18
Identities = 40/52 (76%), Positives = 50/52 (95%)
Frame = -1
Query: 392 MAPGAVLVLRSAHGARAFLYPVVDPSDLQGFEVLSVFHPTDEVINSVIVARK 237
MAPGAVL+LRSAHG RAFLYP+V+P DLQGFEVLS++HPTD+VINSV++++K
Sbjct: 223 MAPGAVLMLRSAHGPRAFLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISKK 274
>ref|NP_176038.1| nicotianamine synthase, putative; protein id: At1g56430.1,
supported by cDNA: gi_20466583 [Arabidopsis thaliana]
gi|27151629|sp|Q9C7X5|NAS4_ARATH Probable nicotianamine
synthase 4
(S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-
adenosyl-methionine 3-amino-3-carboxypropyltransferase
4) gi|25372952|pir||B96606 probable nicotianamine
synathase F13N6.10 [imported] - Arabidopsis thaliana
gi|12323028|gb|AAG51505.1|AC058785_8 nicotianamine
synathase, putative [Arabidopsis thaliana]
gi|20466584|gb|AAM20609.1| nicotianamine synathase,
putative [Arabidopsis thaliana]
gi|22136424|gb|AAM91290.1| nicotianamine synathase,
putative [Arabidopsis thaliana]
Length = 324
Score = 90.9 bits (224), Expect = 3e-18
Identities = 39/52 (75%), Positives = 50/52 (96%)
Frame = -1
Query: 392 MAPGAVLVLRSAHGARAFLYPVVDPSDLQGFEVLSVFHPTDEVINSVIVARK 237
M+PGA+L+LRSAHG RAFLYP+V+P DL+GFEVLSV+HPTDEVINS++++RK
Sbjct: 224 MSPGALLMLRSAHGPRAFLYPIVEPCDLEGFEVLSVYHPTDEVINSIVISRK 275
>dbj|BAA74590.1| nicotianamine synathase [Arabidopsis thaliana]
Length = 320
Score = 90.1 bits (222), Expect = 6e-18
Identities = 45/95 (47%), Positives = 66/95 (69%), Gaps = 9/95 (9%)
Frame = -1
Query: 392 MAPGAVLVLRSAHGARAFLYPVVDPSDLQGFEVLSVFHPTDEVINSVIVARKHSVNPG-- 219
MAPGAV++LRSAHG RAFLYP+VD DL+GFEVL+++HP+D+V+NSV++ARK + G
Sbjct: 223 MAPGAVVMLRSAHGLRAFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIARKLGGSNGAR 282
Query: 218 -------ILLSSKCHELAAEGFNTLNHRNVIDELS 135
+++ C ++ A N +N+I+E S
Sbjct: 283 GSQIGRCVVMPCNCSKVHAILNNRGMEKNLIEEFS 317
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 302,937,451
Number of Sequences: 1393205
Number of extensions: 5630951
Number of successful extensions: 15177
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 14551
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 15131
length of database: 448,689,247
effective HSP length: 106
effective length of database: 301,009,517
effective search space used: 7224228408
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)