KMC003774A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC003774A_C01 KMC003774A_c01
aGAAAATTAGCTTTTTTATCATTCAACAACAACAGGAATTCAAACATAAGAACCAAGCCG
TGCTCATAGCATGGCATAAATCATAACTAACATGCATAATCAAGAATCAATTAAGCATGC
TCATCAAGGGTCAAACTCAACTCATCAATCACATTCCTATGGTTCAACGTGTTAAAACCT
TCAGCAGCCAATTCATGACATTTGCTAGATAGTAAAATCCCTGGATTCACCGAATGCTTC
CTTGCAACAATAACAGAGTTTATAACCTCATCAGTTGGGTGAAAAACTGACAGAACCTCA
AAACCTTGGAGATCAGAAGGATCAACAACCGGATACAGAAACGCTCGAGCTCCATGTGCA
CTCCTCAGAACCAAAACAGCACCAGGAGCCAT


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003774A_C01 KMC003774A_c01
         (392 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

sp|Q9XGI7|NAS_LYCES Nicotianamine synthase (S-adenosyl-L-methion...   107  5e-23
ref|NP_172395.1| nicotianamine synthase, putative; protein id: A...    96  8e-20
emb|CAC82913.1| nicotianamine synthase [Thlaspi caerulescens]          92  2e-18
ref|NP_176038.1| nicotianamine synthase, putative; protein id: A...    91  3e-18
dbj|BAA74590.1| nicotianamine synathase [Arabidopsis thaliana]         90  6e-18

>sp|Q9XGI7|NAS_LYCES Nicotianamine synthase
           (S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-
           adenosyl-methionine 3-amino-3-carboxypropyltransferase)
           (Chloronerva) gi|4753801|emb|CAB42052.1| nicotianamine
           synthase [Lycopersicon esculentum]
          Length = 317

 Score =  107 bits (266), Expect = 5e-23
 Identities = 57/96 (59%), Positives = 69/96 (71%), Gaps = 9/96 (9%)
 Frame = -1

Query: 392 MAPGAVLVLRSAHGARAFLYPVVDPSDLQGFEVLSVFHPTDEVINSVIVARKHSVNPGI- 216
           M+PGA L+LRSAHGARAFLYPV+DP DL+GFEVLSV+HPTDEVINSVI+ARK  V P + 
Sbjct: 221 MSPGATLMLRSAHGARAFLYPVLDPRDLRGFEVLSVYHPTDEVINSVIIARKLPV-PSVP 279

Query: 215 --------LLSSKCHELAAEGFNTLNHRNVIDELSL 132
                   +L SKC       FN LN  N+++E +L
Sbjct: 280 LLDGLGAYVLPSKCACAEIHAFNPLNKMNLVEEFAL 315

>ref|NP_172395.1| nicotianamine synthase, putative; protein id: At1g09240.1,
           supported by cDNA: 37124. [Arabidopsis thaliana]
           gi|27151628|sp|O80483|NAS3_ARATH Nicotianamine synthase
           3
           (S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-
           adenosyl-methionine 3-amino-3-carboxypropyltransferase
           3) (AtNAS3) gi|25372953|pir||C86225 hypothetical protein
           [imported] - Arabidopsis thaliana
           gi|3249099|gb|AAC24082.1| EST gb|T21244 comes from this
           gene. [Arabidopsis thaliana] gi|4220616|dbj|BAA74591.1|
           nicotianamine synthase [Arabidopsis thaliana]
           gi|21593212|gb|AAM65161.1| putative nicotianamine
           synthase [Arabidopsis thaliana]
           gi|22655164|gb|AAM98172.1| putative nicotianamine
           synthase [Arabidopsis thaliana]
          Length = 320

 Score = 96.3 bits (238), Expect = 8e-20
 Identities = 42/55 (76%), Positives = 52/55 (94%)
 Frame = -1

Query: 392 MAPGAVLVLRSAHGARAFLYPVVDPSDLQGFEVLSVFHPTDEVINSVIVARKHSV 228
           MAPGAVL+LRSAHG RAFLYP+V+P DLQGFEVLS++HPTD+VINSV++++KH V
Sbjct: 223 MAPGAVLMLRSAHGPRAFLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISKKHPV 277

>emb|CAC82913.1| nicotianamine synthase [Thlaspi caerulescens]
          Length = 321

 Score = 92.0 bits (227), Expect = 2e-18
 Identities = 40/52 (76%), Positives = 50/52 (95%)
 Frame = -1

Query: 392 MAPGAVLVLRSAHGARAFLYPVVDPSDLQGFEVLSVFHPTDEVINSVIVARK 237
           MAPGAVL+LRSAHG RAFLYP+V+P DLQGFEVLS++HPTD+VINSV++++K
Sbjct: 223 MAPGAVLMLRSAHGPRAFLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISKK 274

>ref|NP_176038.1| nicotianamine synthase, putative; protein id: At1g56430.1,
           supported by cDNA: gi_20466583 [Arabidopsis thaliana]
           gi|27151629|sp|Q9C7X5|NAS4_ARATH Probable nicotianamine
           synthase 4
           (S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-
           adenosyl-methionine 3-amino-3-carboxypropyltransferase
           4) gi|25372952|pir||B96606 probable nicotianamine
           synathase F13N6.10 [imported] - Arabidopsis thaliana
           gi|12323028|gb|AAG51505.1|AC058785_8 nicotianamine
           synathase, putative [Arabidopsis thaliana]
           gi|20466584|gb|AAM20609.1| nicotianamine synathase,
           putative [Arabidopsis thaliana]
           gi|22136424|gb|AAM91290.1| nicotianamine synathase,
           putative [Arabidopsis thaliana]
          Length = 324

 Score = 90.9 bits (224), Expect = 3e-18
 Identities = 39/52 (75%), Positives = 50/52 (96%)
 Frame = -1

Query: 392 MAPGAVLVLRSAHGARAFLYPVVDPSDLQGFEVLSVFHPTDEVINSVIVARK 237
           M+PGA+L+LRSAHG RAFLYP+V+P DL+GFEVLSV+HPTDEVINS++++RK
Sbjct: 224 MSPGALLMLRSAHGPRAFLYPIVEPCDLEGFEVLSVYHPTDEVINSIVISRK 275

>dbj|BAA74590.1| nicotianamine synathase [Arabidopsis thaliana]
          Length = 320

 Score = 90.1 bits (222), Expect = 6e-18
 Identities = 45/95 (47%), Positives = 66/95 (69%), Gaps = 9/95 (9%)
 Frame = -1

Query: 392 MAPGAVLVLRSAHGARAFLYPVVDPSDLQGFEVLSVFHPTDEVINSVIVARKHSVNPG-- 219
           MAPGAV++LRSAHG RAFLYP+VD  DL+GFEVL+++HP+D+V+NSV++ARK   + G  
Sbjct: 223 MAPGAVVMLRSAHGLRAFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIARKLGGSNGAR 282

Query: 218 -------ILLSSKCHELAAEGFNTLNHRNVIDELS 135
                  +++   C ++ A   N    +N+I+E S
Sbjct: 283 GSQIGRCVVMPCNCSKVHAILNNRGMEKNLIEEFS 317

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 302,937,451
Number of Sequences: 1393205
Number of extensions: 5630951
Number of successful extensions: 15177
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 14551
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 15131
length of database: 448,689,247
effective HSP length: 106
effective length of database: 301,009,517
effective search space used: 7224228408
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 GNf098c04 BP074615 1 367
2 MR083e08_f BP082395 30 410
3 GNf070f09 BP072565 31 425




Lotus japonicus
Kazusa DNA Research Institute