Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003750A_C01 KMC003750A_c01
(759 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAC43238.1| calcium binding protein [Sesbania rostrata] 263 3e-69
sp|Q9M7R0|ALL8_OLEEU Calcium-binding allergen Ole e 8 (PCA18/PCA... 181 8e-45
gb|AAF31152.1|AF078680_1 calcium-binding protein [Olea europaea] 178 7e-44
ref|NP_173259.1| calcium-binding protein, putative; protein id: ... 176 4e-43
ref|NP_177504.1| putative calmodulin; protein id: At1g73630.1, s... 173 2e-42
>emb|CAC43238.1| calcium binding protein [Sesbania rostrata]
Length = 172
Score = 263 bits (671), Expect = 3e-69
Identities = 131/171 (76%), Positives = 146/171 (84%), Gaps = 2/171 (1%)
Frame = -3
Query: 724 MATNGNGNGNG--ETNSKASIKPSVYLDDMEELKRVFTRFDTNGDGKISVNELDNVLRSL 551
MATN N E N KPSVY++DM+ELKRVF+RFD NGDGKISVNELDNVLR+L
Sbjct: 1 MATNPTAPENTVTEQNPATKTKPSVYMEDMDELKRVFSRFDANGDGKISVNELDNVLRAL 60
Query: 550 GSGVPPEELKRVMVDLDGDHDGFINLSEFAAFCRSDTADGGASELHDAFELYDQDKNGLI 371
GS VP +EL+RVM DLD D+DGFINL+EFAAFCRSD ADGGASEL +AF+LYDQDKNGLI
Sbjct: 61 GSTVPSDELERVMKDLDTDNDGFINLTEFAAFCRSDAADGGASELREAFDLYDQDKNGLI 120
Query: 370 SAAELCKVLNRLGMNCSEEECHKMIKSVDSDGDGNVNFEEFKKMMTNNHAK 218
SAAELC VLNRLGM CS EECH MIKSVDSDGDGNVNF+EFK+MMTNN++K
Sbjct: 121 SAAELCLVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFDEFKQMMTNNNSK 171
>sp|Q9M7R0|ALL8_OLEEU Calcium-binding allergen Ole e 8 (PCA18/PCA23)
gi|6901652|gb|AAF31151.1|AF078679_1 calcium-binding
protein [Olea europaea]
Length = 171
Score = 181 bits (460), Expect = 8e-45
Identities = 92/156 (58%), Positives = 117/156 (74%), Gaps = 4/156 (2%)
Frame = -3
Query: 685 NSKASIKPSVYLDDMEELKRVFTRFDTNGDGKISVNELDNVLRSLGSGVPPEELKRVMVD 506
N+ + KPSVYL + E++ VF RFD NGDGKIS +EL VL++LGS EE+ R+M +
Sbjct: 4 NTDRNSKPSVYLQEPNEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEE 63
Query: 505 LDGDHDGFINLSEFAAFCRSDT----ADGGASELHDAFELYDQDKNGLISAAELCKVLNR 338
+D D DGFIN+ EFAAF +++T + GG +EL +AFELYDQD NGLIS+ EL K+L R
Sbjct: 64 IDTDKDGFINVQEFAAFVKAETDPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTR 123
Query: 337 LGMNCSEEECHKMIKSVDSDGDGNVNFEEFKKMMTN 230
LG +E +C +MIKSVDSDGDG V+FEEFKKMMTN
Sbjct: 124 LGERYAEHDCVEMIKSVDSDGDGYVSFEEFKKMMTN 159
Score = 41.2 bits (95), Expect = 0.016
Identities = 23/87 (26%), Positives = 38/87 (43%)
Frame = -3
Query: 679 KASIKPSVYLDDMEELKRVFTRFDTNGDGKISVNELDNVLRSLGSGVPPEELKRVMVDLD 500
KA P ELK F +D + +G IS EL +L LG + ++ +D
Sbjct: 82 KAETDPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTRLGERYAEHDCVEMIKSVD 141
Query: 499 GDHDGFINLSEFAAFCRSDTADGGASE 419
D DG+++ EF + + + +E
Sbjct: 142 SDGDGYVSFEEFKKMMTNKSGNNSQAE 168
>gb|AAF31152.1|AF078680_1 calcium-binding protein [Olea europaea]
Length = 171
Score = 178 bits (452), Expect = 7e-44
Identities = 91/156 (58%), Positives = 116/156 (74%), Gaps = 4/156 (2%)
Frame = -3
Query: 685 NSKASIKPSVYLDDMEELKRVFTRFDTNGDGKISVNELDNVLRSLGSGVPPEELKRVMVD 506
N+ + KPSVYL + E++ VF RFD NGDGKIS +EL L++LGS EE+ R+M +
Sbjct: 4 NTDRNSKPSVYLQEPNEVQGVFNRFDANGDGKISGDELACALKALGSNTSKEEIARMMEE 63
Query: 505 LDGDHDGFINLSEFAAFCRSDT----ADGGASELHDAFELYDQDKNGLISAAELCKVLNR 338
+D D DGFIN+ EFAAF +++T + GG +EL +AFELYDQD NGLIS+ EL K+L R
Sbjct: 64 IDTDKDGFINVQEFAAFVKAETDPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTR 123
Query: 337 LGMNCSEEECHKMIKSVDSDGDGNVNFEEFKKMMTN 230
LG +E +C +MIKSVDSDGDG V+FEEFKKMMTN
Sbjct: 124 LGERYAEHDCVEMIKSVDSDGDGYVSFEEFKKMMTN 159
Score = 41.2 bits (95), Expect = 0.016
Identities = 23/87 (26%), Positives = 38/87 (43%)
Frame = -3
Query: 679 KASIKPSVYLDDMEELKRVFTRFDTNGDGKISVNELDNVLRSLGSGVPPEELKRVMVDLD 500
KA P ELK F +D + +G IS EL +L LG + ++ +D
Sbjct: 82 KAETDPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTRLGERYAEHDCVEMIKSVD 141
Query: 499 GDHDGFINLSEFAAFCRSDTADGGASE 419
D DG+++ EF + + + +E
Sbjct: 142 SDGDGYVSFEEFKKMMTNKSGNNSQAE 168
>ref|NP_173259.1| calcium-binding protein, putative; protein id: At1g18210.1,
supported by cDNA: 19462., supported by cDNA:
gi_15027896, supported by cDNA: gi_19310788 [Arabidopsis
thaliana] gi|25295745|pir||A86317 protein T10O22.19
[imported] - Arabidopsis thaliana
gi|8671778|gb|AAF78384.1|AC069551_17 T10O22.19
[Arabidopsis thaliana]
gi|9719735|gb|AAF97837.1|AC034107_20 Strong similarity
to calcium-binding protein (PCA23) from Olea europaea
gb|AF078680 and contains multiple EF-hand PF|00036
domains. ESTs gb|T21585, gb|T21589, gb|T41586,
gb|Z37721, gb|Z29218, gb|AI100607, gb|AI997012,
gb|AV540453, gb|AV544989, gb|AV544493, gb|AV554674 come
from this gene. [Arabidopsis thaliana]
gi|15027897|gb|AAK76479.1| putative calcium-binding
protein [Arabidopsis thaliana]
gi|19310789|gb|AAL85125.1| putative calcium-binding
protein [Arabidopsis thaliana]
Length = 170
Score = 176 bits (445), Expect = 4e-43
Identities = 89/150 (59%), Positives = 116/150 (77%), Gaps = 3/150 (2%)
Frame = -3
Query: 667 KPSVYLDDM---EELKRVFTRFDTNGDGKISVNELDNVLRSLGSGVPPEELKRVMVDLDG 497
KP+ DM EELK+VF +FD+NGDGKISV EL V +++G+ EL RV+ ++D
Sbjct: 10 KPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDT 69
Query: 496 DHDGFINLSEFAAFCRSDTADGGASELHDAFELYDQDKNGLISAAELCKVLNRLGMNCSE 317
D DG+INL EF+ CRS ++ A+E+ DAF+LYDQDKNGLISA+EL +VLNRLGM+CS
Sbjct: 70 DRDGYINLDEFSTLCRSSSS---AAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSV 126
Query: 316 EECHKMIKSVDSDGDGNVNFEEFKKMMTNN 227
E+C +MI VD+DGDGNVNFEEF+KMMT++
Sbjct: 127 EDCTRMIGPVDADGDGNVNFEEFQKMMTSS 156
>ref|NP_177504.1| putative calmodulin; protein id: At1g73630.1, supported by cDNA:
94584. [Arabidopsis thaliana] gi|25295748|pir||C96763
protein calmodulin F25P22.4 [imported] - Arabidopsis
thaliana gi|12324222|gb|AAG52088.1|AC012679_26 putative
calmodulin; 12692-13183 [Arabidopsis thaliana]
Length = 163
Score = 173 bits (439), Expect = 2e-42
Identities = 89/153 (58%), Positives = 117/153 (76%)
Frame = -3
Query: 688 TNSKASIKPSVYLDDMEELKRVFTRFDTNGDGKISVNELDNVLRSLGSGVPPEELKRVMV 509
TN +++ K + DME LK+VF +FD NGDGKISV+EL NV +S+G+ EEL RV+
Sbjct: 4 TNLESTNKSTTPSTDME-LKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLD 62
Query: 508 DLDGDHDGFINLSEFAAFCRSDTADGGASELHDAFELYDQDKNGLISAAELCKVLNRLGM 329
++D D DGFIN EFA CRS ++ A E+ +AF+LYDQ+KNGLIS++E+ KVLNRLGM
Sbjct: 63 EIDIDCDGFINQEEFATICRSSSS---AVEIREAFDLYDQNKNGLISSSEIHKVLNRLGM 119
Query: 328 NCSEEECHKMIKSVDSDGDGNVNFEEFKKMMTN 230
CS E+C +MI VD+DGDGNVNFEEF+KMM++
Sbjct: 120 TCSVEDCVRMIGHVDTDGDGNVNFEEFQKMMSS 152
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 670,931,982
Number of Sequences: 1393205
Number of extensions: 16177492
Number of successful extensions: 213211
Number of sequences better than 10.0: 8874
Number of HSP's better than 10.0 without gapping: 106921
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174035
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 37158613404
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
