Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003713A_C01 KMC003713A_c01
(697 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|P42043|HMZ1_ARATH Ferrochelatase I, chloroplast/mitochondrial... 119 4e-26
sp|P42045|HEMZ_HORVU Ferrochelatase II, chloroplast precursor (P... 115 6e-25
pir||T04373 probable ferrochelatase (EC 4.99.1.1) - barley gi|24... 115 6e-25
sp|P42044|HEMZ_CUCSA Ferrochelatase II, chloroplast precursor (P... 115 8e-25
pir||T07739 probable ferrochelatase (EC 4.99.1.1) - potato gi|30... 73 4e-12
>sp|P42043|HMZ1_ARATH Ferrochelatase I, chloroplast/mitochondrial precursor (Protoheme
ferro-lyase) (Heme synthetase) gi|1076325|pir||A54125
ferrochelatase (EC 4.99.1.1) precursor, chloroplast -
Arabidopsis thaliana gi|511081|emb|CAA51819.1|
ferrochelatase [Arabidopsis thaliana]
gi|2597828|emb|CAA73809.1| ferrochelatase-I [Arabidopsis
thaliana] gi|5107825|gb|AAD40138.1|AF149413_19
Arabidopsis thaliana ferrochelatase-I (SW:P42043); Pfam
PF00762, Score=654, E=7.9e-193, N=1
Length = 466
Score = 119 bits (298), Expect = 4e-26
Identities = 77/140 (55%), Positives = 88/140 (62%), Gaps = 3/140 (2%)
Frame = -3
Query: 695 QSRVGPVQWLKPYTDEVLVELGKKGVKSLLAVRSELCE*AY*DP*RN*HGVQGVGS*IWC 516
QSRVGPVQWLKPYTDEVLV+LGK GVKSLLAV + +
Sbjct: 327 QSRVGPVQWLKPYTDEVLVDLGKSGVKSLLAVPVSFVSEHIETLEEIDMEYRELALESGV 386
Query: 515 QELGTCPCPRPLRPSFITDLADAVIEALPSATAMYAPSST--NEDVEQ-DPVRFFVKMFF 345
+ G P L PSFITDLADAVIE+LPSA AM P++ +ED E D + VKMFF
Sbjct: 387 ENWGRVPA-LGLTPSFITDLADAVIESLPSAEAMSNPNAVVDSEDSESSDAFSYIVKMFF 445
Query: 344 GSIFAFFLLLSPKMINAFKN 285
GSI AF LLLSPKM +AF+N
Sbjct: 446 GSILAFVLLLSPKMFHAFRN 465
Score = 72.4 bits (176), Expect = 6e-12
Identities = 34/37 (91%), Positives = 36/37 (96%)
Frame = -1
Query: 595 VSFVSEHIETLEEIDMEYKELALESGVKNWARVPALG 485
VSFVSEHIETLEEIDMEY+ELALESGV+NW RVPALG
Sbjct: 360 VSFVSEHIETLEEIDMEYRELALESGVENWGRVPALG 396
>sp|P42045|HEMZ_HORVU Ferrochelatase II, chloroplast precursor (Protoheme ferro-lyase)
(Heme synthetase) gi|7437215|pir||T05736 probable
ferrochelatase (EC 4.99.1.1) - barley
gi|474966|dbj|BAA05101.1| ferrochelatase [Hordeum
vulgare subsp. vulgare]
Length = 484
Score = 115 bits (288), Expect = 6e-25
Identities = 68/137 (49%), Positives = 85/137 (61%)
Frame = -3
Query: 695 QSRVGPVQWLKPYTDEVLVELGKKGVKSLLAVRSELCE*AY*DP*RN*HGVQGVGS*IWC 516
QSRVGPVQWLKPYTDEVLVELG+KGVKSLLAV + +
Sbjct: 346 QSRVGPVQWLKPYTDEVLVELGQKGVKSLLAVPVSFVSEHIETLEEIDMEYRELALESGI 405
Query: 515 QELGTCPCPRPLRPSFITDLADAVIEALPSATAMYAPSSTNEDVEQDPVRFFVKMFFGSI 336
+ G P SFI+DLADAV+EALPSA+AM + D + D + + KMF GS+
Sbjct: 406 ENWGRVPA-LGCTSSFISDLADAVVEALPSASAMATRKVKDTDSDMDMMHYLTKMFLGSV 464
Query: 335 FAFFLLLSPKMINAFKN 285
AFFLLLSP++++AF+N
Sbjct: 465 LAFFLLLSPRLVSAFRN 481
Score = 72.0 bits (175), Expect = 7e-12
Identities = 33/37 (89%), Positives = 36/37 (97%)
Frame = -1
Query: 595 VSFVSEHIETLEEIDMEYKELALESGVKNWARVPALG 485
VSFVSEHIETLEEIDMEY+ELALESG++NW RVPALG
Sbjct: 379 VSFVSEHIETLEEIDMEYRELALESGIENWGRVPALG 415
>pir||T04373 probable ferrochelatase (EC 4.99.1.1) - barley
gi|2460251|gb|AAB71887.1| ferrochelatase [Hordeum
vulgare]
Length = 484
Score = 115 bits (288), Expect = 6e-25
Identities = 68/137 (49%), Positives = 85/137 (61%)
Frame = -3
Query: 695 QSRVGPVQWLKPYTDEVLVELGKKGVKSLLAVRSELCE*AY*DP*RN*HGVQGVGS*IWC 516
QSRVGPVQWLKPYTDEVLVELG+KGVKSLLAV + +
Sbjct: 346 QSRVGPVQWLKPYTDEVLVELGQKGVKSLLAVPVSFVSEHIETLEEIDMEYRELALESGI 405
Query: 515 QELGTCPCPRPLRPSFITDLADAVIEALPSATAMYAPSSTNEDVEQDPVRFFVKMFFGSI 336
+ G P SFI+DLADAV+EALPSA+AM + D + D + + KMF GS+
Sbjct: 406 ENWGRVPA-LGCTSSFISDLADAVVEALPSASAMATRKVKDTDSDMDMMHYLTKMFLGSV 464
Query: 335 FAFFLLLSPKMINAFKN 285
AFFLLLSP++++AF+N
Sbjct: 465 LAFFLLLSPRLVSAFRN 481
Score = 72.0 bits (175), Expect = 7e-12
Identities = 33/37 (89%), Positives = 36/37 (97%)
Frame = -1
Query: 595 VSFVSEHIETLEEIDMEYKELALESGVKNWARVPALG 485
VSFVSEHIETLEEIDMEY+ELALESG++NW RVPALG
Sbjct: 379 VSFVSEHIETLEEIDMEYRELALESGIENWGRVPALG 415
>sp|P42044|HEMZ_CUCSA Ferrochelatase II, chloroplast precursor (Protoheme ferro-lyase)
(Heme synthetase) gi|7437219|pir||T10246 ferrochelatase
(EC 4.99.1.1) - cucumber gi|474968|dbj|BAA05102.1|
ferrochelatase [Cucumis sativus]
Length = 514
Score = 115 bits (287), Expect = 8e-25
Identities = 73/144 (50%), Positives = 88/144 (60%), Gaps = 4/144 (2%)
Frame = -3
Query: 695 QSRVGPVQWLKPYTDEVLVELGKKGVKSLLAVR----SELCE*AY*DP*RN*HGVQGVGS 528
QSRVGPVQWLKPYTDEVLVELG+KG+KSLLAV SE E H G
Sbjct: 352 QSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGI 411
Query: 527 *IWCQELGTCPCPRPLRPSFITDLADAVIEALPSATAMYAPSSTNEDVEQDPVRFFVKMF 348
W G P SFI+DLADAVIEALPSATA+ +S+ + + DP + +K+
Sbjct: 412 QNW----GRVPALN-CNSSFISDLADAVIEALPSATALAPHTSSTDADDHDPFLYAIKLL 466
Query: 347 FGSIFAFFLLLSPKMINAFKNHVI 276
FGS+ AF LLLSPK F+N+ +
Sbjct: 467 FGSVLAFILLLSPKAFMVFRNNFL 490
Score = 68.9 bits (167), Expect = 6e-11
Identities = 32/36 (88%), Positives = 34/36 (93%)
Frame = -1
Query: 595 VSFVSEHIETLEEIDMEYKELALESGVKNWARVPAL 488
VSFVSEHIETLEEIDMEYK LALESG++NW RVPAL
Sbjct: 385 VSFVSEHIETLEEIDMEYKHLALESGIQNWGRVPAL 420
>pir||T07739 probable ferrochelatase (EC 4.99.1.1) - potato
gi|3097271|emb|CAA06705.1| ferrochelatase [Solanum
tuberosum]
Length = 543
Score = 72.8 bits (177), Expect = 4e-12
Identities = 33/37 (89%), Positives = 37/37 (99%)
Frame = -1
Query: 595 VSFVSEHIETLEEIDMEYKELALESGVKNWARVPALG 485
+SFVSEHIETLEEID+EYKELALESG++NWARVPALG
Sbjct: 357 ISFVSEHIETLEEIDVEYKELALESGIQNWARVPALG 393
Score = 70.1 bits (170), Expect = 3e-11
Identities = 48/110 (43%), Positives = 58/110 (52%), Gaps = 16/110 (14%)
Frame = -3
Query: 695 QSRVGPVQWLKPYTDEVLVELGKKGVKSLLAV----------------RSELCE*AY*DP 564
QSRVGPV+WLKPYTDE ++ELGKKGVKS+LAV E E A
Sbjct: 323 QSRVGPVEWLKPYTDETIIELGKKGVKSILAVPIISFVSEHIETLEEIDVEYKELALESG 382
Query: 563 *RN*HGVQGVGS*IWCQELGTCPCPRPLRPSFITDLADAVIEALPSATAM 414
+N V +G + P+FI+DLADAVIE+LP AM
Sbjct: 383 IQNWARVPALG----------------VEPTFISDLADAVIESLPYVGAM 416
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 570,505,255
Number of Sequences: 1393205
Number of extensions: 11616066
Number of successful extensions: 29933
Number of sequences better than 10.0: 140
Number of HSP's better than 10.0 without gapping: 28772
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 29918
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 31684559424
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
