Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003617A_C01 KMC003617A_c01
(502 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_187954.1| branched chain alpha-keto acid dehydrogenase E1... 98 5e-20
ref|NP_175947.1| branched-chain alpha-keto acid decarboxylase E1... 97 1e-19
pir||T51835 3-methyl-2-oxobutanoate dehydrogenase (lipoamide) (E... 97 1e-19
gb|AAA51410.1| branched chain alpha-keto acid dehydrogenase E1-b... 70 1e-11
ref|NP_776932.1| branched chain keto acid dehydrogenase E1, beta... 70 2e-11
>ref|NP_187954.1| branched chain alpha-keto acid dehydrogenase E1 beta subunit;
protein id: At3g13450.1, supported by cDNA: gi_7021285
[Arabidopsis thaliana]
gi|7021286|gb|AAF35281.1|AF145452_1 branched chain
alpha-keto acid dehydrogenase E1 beta subunit
[Arabidopsis thaliana] gi|9280297|dbj|BAB01752.1|
branched chain alpha-keto acid dehydrogenase E1 beta
subunit [Arabidopsis thaliana]
Length = 358
Score = 98.2 bits (243), Expect = 5e-20
Identities = 45/49 (91%), Positives = 48/49 (97%)
Frame = -3
Query: 500 SIVERCFSRLEAPVARVCGLDTPFPLVFEPFYMPTKNKILDAIKSTVNY 354
+IVERCF RLEAPV+RVCGLDTPFPLVFEPFYMPTKNKILDAI+STVNY
Sbjct: 310 TIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIRSTVNY 358
>ref|NP_175947.1| branched-chain alpha-keto acid decarboxylase E1 beta subunit;
protein id: At1g55510.1, supported by cDNA: gi_3746567
[Arabidopsis thaliana] gi|25284437|pir||D96597
hypothetical protein T5A14.9 [imported] - Arabidopsis
thaliana gi|4204270|gb|AAD10651.1| branched-chain
alpha-keto acid decarboxylase E1 beta subunit
[Arabidopsis thaliana]
Length = 352
Score = 97.1 bits (240), Expect = 1e-19
Identities = 44/49 (89%), Positives = 48/49 (97%)
Frame = -3
Query: 500 SIVERCFSRLEAPVARVCGLDTPFPLVFEPFYMPTKNKILDAIKSTVNY 354
+I+ERCF +LEAPV+RVCGLDTPFPLVFEPFYMPTKNKILDAIKSTVNY
Sbjct: 304 TILERCFLKLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKSTVNY 352
>pir||T51835 3-methyl-2-oxobutanoate dehydrogenase (lipoamide) (EC 1.2.4.4) beta
chain [imported] - Arabidopsis thaliana
gi|3746568|gb|AAC64005.1| branched-chain alpha-keto acid
decarboxylase E1 beta subunit [Arabidopsis thaliana]
Length = 352
Score = 97.1 bits (240), Expect = 1e-19
Identities = 44/49 (89%), Positives = 48/49 (97%)
Frame = -3
Query: 500 SIVERCFSRLEAPVARVCGLDTPFPLVFEPFYMPTKNKILDAIKSTVNY 354
+I+ERCF +LEAPV+RVCGLDTPFPLVFEPFYMPTKNKILDAIKSTVNY
Sbjct: 304 TILERCFLKLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKSTVNY 352
>gb|AAA51410.1| branched chain alpha-keto acid dehydrogenase E1-beta subunit
Length = 369
Score = 70.1 bits (170), Expect = 1e-11
Identities = 28/49 (57%), Positives = 38/49 (77%)
Frame = -3
Query: 500 SIVERCFSRLEAPVARVCGLDTPFPLVFEPFYMPTKNKILDAIKSTVNY 354
++ E+CF LEAP++RVCG DTPFP +FEPFY+P K K DA++ +NY
Sbjct: 321 TVQEQCFLNLEAPISRVCGYDTPFPHIFEPFYIPDKWKCYDALRKMINY 369
>ref|NP_776932.1| branched chain keto acid dehydrogenase E1, beta polypeptide (maple
syrup urine disease) [Bos taurus]
gi|129033|sp|P21839|ODBB_BOVIN 2-oxoisovalerate
dehydrogenase beta subunit, mitochondrial precursor
(Branched-chain alpha-keto acid dehydrogenase E1
component beta chain) (BCKDH E1-beta)
gi|108478|pir||A34267 3-methyl-2-oxobutanoate
dehydrogenase (lipoamide) (EC 1.2.4.4) E1 beta chain
precursor - bovine gi|506803|gb|AAA30407.1|
3-methyl-2-oxobutanoate dehydrogenase
Length = 392
Score = 69.7 bits (169), Expect = 2e-11
Identities = 28/49 (57%), Positives = 37/49 (75%)
Frame = -3
Query: 500 SIVERCFSRLEAPVARVCGLDTPFPLVFEPFYMPTKNKILDAIKSTVNY 354
++ E CF LEAP++RVCG DTPFP +FEPFY+P K K DA++ +NY
Sbjct: 344 TVQEECFLNLEAPISRVCGYDTPFPHIFEPFYIPDKWKCYDALRKMINY 392
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 402,151,801
Number of Sequences: 1393205
Number of extensions: 7700761
Number of successful extensions: 14714
Number of sequences better than 10.0: 136
Number of HSP's better than 10.0 without gapping: 14447
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 14684
length of database: 448,689,247
effective HSP length: 114
effective length of database: 289,863,877
effective search space used: 15072921604
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
