Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003541A_C01 KMC003541A_c01
(688 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAM64754.1| unknown [Arabidopsis thaliana] 77 5e-31
dbj|BAC42851.1| unknown protein [Arabidopsis thaliana] 78 5e-30
ref|NP_680183.1| similar to putative hydrolase; protein id: At5g... 62 3e-25
dbj|BAC21318.1| hydrolase-like protein [Oryza sativa (japonica c... 49 4e-18
gb|AAO13465.1| Unknown protein [Oryza sativa (japonica cultivar-... 49 6e-17
>gb|AAM64754.1| unknown [Arabidopsis thaliana]
Length = 250
Score = 77.0 bits (188), Expect(3) = 5e-31
Identities = 42/77 (54%), Positives = 52/77 (66%), Gaps = 1/77 (1%)
Frame = -2
Query: 687 ASSGVNMKKSDNAALLQRAELEKIEDLMLPVTPLQLRKLMTLAVSKR-VQFVPDFFLKDY 511
ASSG+NM+K D +LLQR+ E IE +MLP T + R LM LA S R V+ PD D
Sbjct: 97 ASSGINMRKCDGESLLQRSNCECIEKVMLPSTATEFRTLMALASSWRLVRMFPDALWNDV 156
Query: 510 LNKLYKENRKEKMELAK 460
+N LYK+NRKEK+EL K
Sbjct: 157 INNLYKKNRKEKIELLK 173
Score = 57.4 bits (137), Expect(3) = 5e-31
Identities = 25/43 (58%), Positives = 34/43 (78%)
Frame = -1
Query: 460 KLISLGREDTSNLSSLQQEVLIVWGENDRIFPVQMAHELKEAI 332
K ++ GR + N+ SL QEVLIVWG+ D+IFPV+MA+ELKE +
Sbjct: 173 KGVTFGRSENLNIDSLSQEVLIVWGDKDQIFPVKMAYELKEIL 215
Score = 42.7 bits (99), Expect(3) = 5e-31
Identities = 19/33 (57%), Positives = 24/33 (72%)
Frame = -3
Query: 323 KARLELIKDASHVPQLEKPVEFNNIILNFLNAS 225
K +LE+I + SHVPQ+E EFNNI+L FL S
Sbjct: 218 KTKLEIIDNTSHVPQIECAQEFNNIVLRFLKGS 250
>dbj|BAC42851.1| unknown protein [Arabidopsis thaliana]
Length = 204
Score = 78.2 bits (191), Expect(3) = 5e-30
Identities = 35/74 (47%), Positives = 52/74 (69%)
Frame = -2
Query: 687 ASSGVNMKKSDNAALLQRAELEKIEDLMLPVTPLQLRKLMTLAVSKRVQFVPDFFLKDYL 508
ASSGVN+++SDN A + RA+ +I+++MLP + LR+ + SKR+ +VPDF L D+
Sbjct: 48 ASSGVNLRRSDNEAFIARAKCHRIKEVMLPASATDLRRFSGMVSSKRLDYVPDFVLNDFC 107
Query: 507 NKLYKENRKEKMEL 466
K+Y E R+EK EL
Sbjct: 108 QKMYSEKREEKAEL 121
Score = 58.5 bits (140), Expect(3) = 5e-30
Identities = 20/41 (48%), Positives = 37/41 (89%)
Frame = -1
Query: 454 ISLGREDTSNLSSLQQEVLIVWGENDRIFPVQMAHELKEAI 332
+S+G++D +N+S +QQ+V+++WGE D++FP++MAH+LKE +
Sbjct: 125 LSIGKDDKTNVSPIQQDVMLIWGEQDQVFPLKMAHDLKEML 165
Score = 37.0 bits (84), Expect(3) = 5e-30
Identities = 15/30 (50%), Positives = 22/30 (73%)
Frame = -3
Query: 323 KARLELIKDASHVPQLEKPVEFNNIILNFL 234
KA L++I+ SH+PQ EK EFN +++FL
Sbjct: 168 KATLKVIQKTSHIPQTEKSKEFNGFVMSFL 197
>ref|NP_680183.1| similar to putative hydrolase; protein id: At5g21950.1 [Arabidopsis
thaliana] gi|29294062|gb|AAO73899.1| hydrolase,
alpha/beta fold family [Arabidopsis thaliana]
Length = 300
Score = 62.0 bits (149), Expect(3) = 3e-25
Identities = 27/59 (45%), Positives = 42/59 (70%)
Frame = -2
Query: 687 ASSGVNMKKSDNAALLQRAELEKIEDLMLPVTPLQLRKLMTLAVSKRVQFVPDFFLKDY 511
ASSGVN+++SDN A + RA+ +I+++MLP + LR+ + SKR+ +VPDF L D+
Sbjct: 152 ASSGVNLRRSDNEAFIARAKCHRIKEVMLPASATDLRRFSGMVSSKRLDYVPDFVLNDF 210
Score = 58.5 bits (140), Expect(3) = 3e-25
Identities = 20/41 (48%), Positives = 37/41 (89%)
Frame = -1
Query: 454 ISLGREDTSNLSSLQQEVLIVWGENDRIFPVQMAHELKEAI 332
+S+G++D +N+S +QQ+V+++WGE D++FP++MAH+LKE +
Sbjct: 221 LSIGKDDKTNVSPIQQDVMLIWGEQDQVFPLKMAHDLKEML 261
Score = 37.0 bits (84), Expect(3) = 3e-25
Identities = 15/30 (50%), Positives = 22/30 (73%)
Frame = -3
Query: 323 KARLELIKDASHVPQLEKPVEFNNIILNFL 234
KA L++I+ SH+PQ EK EFN +++FL
Sbjct: 264 KATLKVIQKTSHIPQTEKSKEFNGFVMSFL 293
>dbj|BAC21318.1| hydrolase-like protein [Oryza sativa (japonica cultivar-group)]
Length = 336
Score = 49.3 bits (116), Expect(3) = 4e-18
Identities = 31/83 (37%), Positives = 48/83 (57%), Gaps = 2/83 (2%)
Frame = -2
Query: 687 ASSGVNMKKSDNAALLQRAELEKIEDLMLPVTPLQLRKLMTLAV--SKRVQFVPDFFLKD 514
ASS + +D+ ALL+R E++ED+MLP TP ++R+L+ LA +R F P F L+D
Sbjct: 153 ASSDLLKADADDRALLRRGGAERVEDVMLPRTPERMRRLLGLAYHRPRRFSFTPAFVLRD 212
Query: 513 YLNKLYKENRKEKMELAKSSYHL 445
L + E + L + + HL
Sbjct: 213 ----LAQLTSTEALTLCECNCHL 231
Score = 44.7 bits (104), Expect(3) = 4e-18
Identities = 19/41 (46%), Positives = 31/41 (75%)
Frame = -1
Query: 460 KLISLGREDTSNLSSLQQEVLIVWGENDRIFPVQMAHELKE 338
K I+LG ++ L+ L QEVL++WGE+D+IFP++ A E+ +
Sbjct: 255 KGITLGDKEKFQLTPLPQEVLVLWGEHDQIFPIEKAFEVAQ 295
Score = 39.3 bits (90), Expect(3) = 4e-18
Identities = 17/29 (58%), Positives = 21/29 (71%)
Frame = -3
Query: 320 ARLELIKDASHVPQLEKPVEFNNIILNFL 234
ARLE+IK+ H+PQ E P FN +LNFL
Sbjct: 300 ARLEIIKNTGHMPQEEDPKRFNEALLNFL 328
>gb|AAO13465.1| Unknown protein [Oryza sativa (japonica cultivar-group)]
Length = 328
Score = 48.9 bits (115), Expect(3) = 6e-17
Identities = 27/80 (33%), Positives = 48/80 (59%), Gaps = 4/80 (5%)
Frame = -2
Query: 687 ASSGVNMKKSDNAALLQRAE--LEKIEDLMLPVTPLQLRKLMTLAVSKRVQ--FVPDFFL 520
ASS + +D+ L+RA +E+++LP P +RKL+ +A + +PDF L
Sbjct: 161 ASSDLLKTAADDRGFLKRAGDGWSGVEEVLLPAEPAAMRKLLEMASCRPPPPVLMPDFIL 220
Query: 519 KDYLNKLYKENRKEKMELAK 460
+D++ KL+ ENR++ ++L K
Sbjct: 221 RDFIQKLFTENREQLIQLFK 240
Score = 41.2 bits (95), Expect(3) = 6e-17
Identities = 18/33 (54%), Positives = 23/33 (69%)
Frame = -3
Query: 323 KARLELIKDASHVPQLEKPVEFNNIILNFLNAS 225
KAR+E+I H PQLE P FN I+L+FL A+
Sbjct: 284 KARVEIISKTGHAPQLEDPTRFNKILLDFLLAT 316
Score = 38.9 bits (89), Expect(3) = 6e-17
Identities = 15/35 (42%), Positives = 26/35 (73%)
Frame = -1
Query: 436 DTSNLSSLQQEVLIVWGENDRIFPVQMAHELKEAI 332
D ++ + QEVLIVWGE D++FPV+ A+ ++ ++
Sbjct: 247 DKFPVTPISQEVLIVWGEQDQLFPVEKAYAVQSSL 281
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 583,463,171
Number of Sequences: 1393205
Number of extensions: 12720313
Number of successful extensions: 44140
Number of sequences better than 10.0: 94
Number of HSP's better than 10.0 without gapping: 40528
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 43711
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 30835865868
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
