Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003305A_C01 KMC003305A_c01
(1311 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAD16897.1| glutamyl-tRNA reductase precursor [Glycine max] 553 e-156
sp|P93111|HMA1_CUCSA Glutamyl-tRNA reductase 1, chloroplast prec... 538 e-152
ref|NP_176125.1| glutamyl-tRNA reductase, putative; protein id: ... 501 e-140
gb|AAA19118.1| glutamyl-tRNA reductase 496 e-139
sp|P49294|HMA2_ARATH Glutamyl-tRNA reductase 2, chloroplast prec... 467 e-130
>gb|AAD16897.1| glutamyl-tRNA reductase precursor [Glycine max]
Length = 542
Score = 553 bits (1426), Expect = e-156
Identities = 282/316 (89%), Positives = 304/316 (95%), Gaps = 1/316 (0%)
Frame = -1
Query: 1311 GFGRNISGLFKHAITVGKRVRAETNIAAGAVSVSSAAVELAFMKLPGTSH-DARMLVIGA 1135
GFGRNISGLFKHAITVGKRVR ETNIAAGAVSVSSAAVELA MKLP SH +ARMLVIGA
Sbjct: 225 GFGRNISGLFKHAITVGKRVRTETNIAAGAVSVSSAAVELALMKLPEASHANARMLVIGA 284
Query: 1134 GKMGKLVIKHLVAKGCTKMVVVNRTEERVAAIREELKDVEIIYKPLSEMLTCVGEADVVF 955
GKMGKLVIKHLVAKGCTKMVVVNR+EERVAAIREE+KDVEIIYKPLSEMLTC+GEADVVF
Sbjct: 285 GKMGKLVIKHLVAKGCTKMVVVNRSEERVAAIREEIKDVEIIYKPLSEMLTCIGEADVVF 344
Query: 954 TSTASETPLFMKDHVKDLPPASQDVGGQRIFIDISVPRNVDSCVSDLDSVRVYNVDDLKE 775
TSTASE PLF+KD VK+LPPA+ +VGG+R+F+DISVPRNV SC+SDL+SVRVYNVDDLKE
Sbjct: 345 TSTASENPLFLKDDVKELPPATDEVGGRRLFVDISVPRNVGSCLSDLESVRVYNVDDLKE 404
Query: 774 VVAANKEDRLRKAMEAQTIIAEESKQFEAWRDSLETVPTIKKLRAYAERIRVAELDKCLG 595
VVAANKEDRLRKAMEAQ II EESKQFEAWRDSLETVPTIKKLRAYAERIR+AEL+KCLG
Sbjct: 405 VVAANKEDRLRKAMEAQAIIGEESKQFEAWRDSLETVPTIKKLRAYAERIRLAELEKCLG 464
Query: 594 KMGEDISKKMRRAVDDLSRGMVNRLLHGPMQHLRCDGSDSRTLNETLENMHALNRMFSLE 415
KMG+DI+KK +RAVDDLSRG+VN+LLHGPMQHLRCDGSDSRTL+ETLENMHALNRMF+LE
Sbjct: 465 KMGDDINKKTQRAVDDLSRGIVNKLLHGPMQHLRCDGSDSRTLSETLENMHALNRMFNLE 524
Query: 414 TEISVLEQKIRAKVEQ 367
TEISVLEQKIRAKVEQ
Sbjct: 525 TEISVLEQKIRAKVEQ 540
>sp|P93111|HMA1_CUCSA Glutamyl-tRNA reductase 1, chloroplast precursor (GluTR)
gi|7433724|pir||T10186 glutamyl-tRNA reductase (EC
1.2.1.-) - cucumber gi|1694926|dbj|BAA08910.1|
glutamyl-tRNA reductase [Cucumis sativus]
Length = 552
Score = 538 bits (1387), Expect = e-152
Identities = 276/319 (86%), Positives = 296/319 (92%), Gaps = 1/319 (0%)
Frame = -1
Query: 1311 GFGRNISGLFKHAITVGKRVRAETNIAAGAVSVSSAAVELAFMKLPGTSH-DARMLVIGA 1135
GFGRNISGLFKHAITVGKRVR ETNIAAGAVSVSSAAVELA MKLP SH ARMLVIGA
Sbjct: 234 GFGRNISGLFKHAITVGKRVRTETNIAAGAVSVSSAAVELALMKLPEPSHATARMLVIGA 293
Query: 1134 GKMGKLVIKHLVAKGCTKMVVVNRTEERVAAIREELKDVEIIYKPLSEMLTCVGEADVVF 955
GKMGKLVIKHLVAKGCTKMVVVNR+EERV AIREE+KDVEIIYKPL+EML+C EADV+F
Sbjct: 294 GKMGKLVIKHLVAKGCTKMVVVNRSEERVTAIREEMKDVEIIYKPLTEMLSCTAEADVIF 353
Query: 954 TSTASETPLFMKDHVKDLPPASQDVGGQRIFIDISVPRNVDSCVSDLDSVRVYNVDDLKE 775
TSTASE+ LF K+ VKDLPP DVGG R+FIDISVPRNV +C+++L+ VRVYNVDDLKE
Sbjct: 354 TSTASESLLFTKEQVKDLPPVGHDVGGLRLFIDISVPRNVGACINNLEDVRVYNVDDLKE 413
Query: 774 VVAANKEDRLRKAMEAQTIIAEESKQFEAWRDSLETVPTIKKLRAYAERIRVAELDKCLG 595
VVAANKEDRLRKAMEAQ+II EESKQFEAWRDSLETVPTIKKLRAYAERIR AEL+KCL
Sbjct: 414 VVAANKEDRLRKAMEAQSIITEESKQFEAWRDSLETVPTIKKLRAYAERIRTAELEKCLS 473
Query: 594 KMGEDISKKMRRAVDDLSRGMVNRLLHGPMQHLRCDGSDSRTLNETLENMHALNRMFSLE 415
KMG+DI KK RRAVDDLSRG+VN+LLHGPMQHLRCDGSDSRTL+ETLENMHALNRMFSLE
Sbjct: 474 KMGDDIPKKTRRAVDDLSRGIVNKLLHGPMQHLRCDGSDSRTLSETLENMHALNRMFSLE 533
Query: 414 TEISVLEQKIRAKVEQNQK 358
TEI+VLEQKIRAKVEQNQK
Sbjct: 534 TEIAVLEQKIRAKVEQNQK 552
>ref|NP_176125.1| glutamyl-tRNA reductase, putative; protein id: At1g58290.1, supported
by cDNA: gi_18176438, supported by cDNA: gi_20465534
[Arabidopsis thaliana] gi|20141560|sp|P42804|HMA1_ARATH
Glutamyl-tRNA reductase 1, chloroplast precursor (GluTR)
gi|25286553|pir||E96616 hypothetical protein F19C14.9
[imported] - Arabidopsis thaliana
gi|8979944|gb|AAF82258.1|AC008051_9 Identical to
glutamyl-tRNA reductase (hemA) from Arabidopsis thaliana
gb|U03774 and contains a Glutaminyl-tRNA reductase
PF|00745 domain. ESTs gb|H37325, gb|R90339, gb|AI992625,
gb|N96248, gb|U74113 come from this gene
gi|18176439|gb|AAL60044.1| putative glutamyl-tRNA
reductase [Arabidopsis thaliana]
gi|20465535|gb|AAM20250.1| putative glutamyl-tRNA
reductase [Arabidopsis thaliana]
Length = 543
Score = 501 bits (1289), Expect = e-140
Identities = 255/319 (79%), Positives = 287/319 (89%), Gaps = 1/319 (0%)
Frame = -1
Query: 1311 GFGRNISGLFKHAITVGKRVRAETNIAAGAVSVSSAAVELAFMKLPGTSH-DARMLVIGA 1135
GFGRNISGLFKHAITVGKRVR ETNIA+GAVSVSSAAVELA MKLP +S+ ARM VIGA
Sbjct: 227 GFGRNISGLFKHAITVGKRVRTETNIASGAVSVSSAAVELALMKLPQSSNVSARMCVIGA 286
Query: 1134 GKMGKLVIKHLVAKGCTKMVVVNRTEERVAAIREELKDVEIIYKPLSEMLTCVGEADVVF 955
GKMGKLVIKHL+AKGCTK+VVVNR+EERV+AIREE+ +EIIY+PL EML C EADVVF
Sbjct: 287 GKMGKLVIKHLMAKGCTKVVVVNRSEERVSAIREEMPGIEIIYRPLDEMLACASEADVVF 346
Query: 954 TSTASETPLFMKDHVKDLPPASQDVGGQRIFIDISVPRNVDSCVSDLDSVRVYNVDDLKE 775
TSTASETPLF+K+HV++LP AS +VGG R F+DISVPRNV SCV ++++ RVYNVDDLKE
Sbjct: 347 TSTASETPLFLKEHVENLPQASPEVGGLRHFVDISVPRNVGSCVGEVETARVYNVDDLKE 406
Query: 774 VVAANKEDRLRKAMEAQTIIAEESKQFEAWRDSLETVPTIKKLRAYAERIRVAELDKCLG 595
VVAANKEDR+RKAMEAQTII EES QFEAWRDSLETVPTIKKLRAYAERIRVAEL+KC+
Sbjct: 407 VVAANKEDRMRKAMEAQTIITEESTQFEAWRDSLETVPTIKKLRAYAERIRVAELEKCMS 466
Query: 594 KMGEDISKKMRRAVDDLSRGMVNRLLHGPMQHLRCDGSDSRTLNETLENMHALNRMFSLE 415
KMG+DI+KK RAVDDLSRG+VNR LHGPMQHLRCDGSDSRTL+ETLENMHALNRM+ LE
Sbjct: 467 KMGDDINKKTTRAVDDLSRGIVNRFLHGPMQHLRCDGSDSRTLSETLENMHALNRMYGLE 526
Query: 414 TEISVLEQKIRAKVEQNQK 358
+I LE+K++A EQ QK
Sbjct: 527 KDI--LEEKLKAMAEQQQK 543
>gb|AAA19118.1| glutamyl-tRNA reductase
Length = 543
Score = 496 bits (1277), Expect = e-139
Identities = 253/319 (79%), Positives = 285/319 (89%), Gaps = 1/319 (0%)
Frame = -1
Query: 1311 GFGRNISGLFKHAITVGKRVRAETNIAAGAVSVSSAAVELAFMKLPGTSH-DARMLVIGA 1135
GFGRNISGLFKHAITVGKRVR ETNIA+GAVSVSSAAVELA MKLP +S+ ARM VIGA
Sbjct: 227 GFGRNISGLFKHAITVGKRVRTETNIASGAVSVSSAAVELALMKLPQSSNVSARMCVIGA 286
Query: 1134 GKMGKLVIKHLVAKGCTKMVVVNRTEERVAAIREELKDVEIIYKPLSEMLTCVGEADVVF 955
GKMG LVIKHL+AKGCTK+VVVNR+EERV+AIREE+ +EIIY+PL EML C EADVVF
Sbjct: 287 GKMGLLVIKHLMAKGCTKVVVVNRSEERVSAIREEMPGIEIIYRPLDEMLACASEADVVF 346
Query: 954 TSTASETPLFMKDHVKDLPPASQDVGGQRIFIDISVPRNVDSCVSDLDSVRVYNVDDLKE 775
TSTASETPLF+K+HV++LP AS +VGG R F+DISVPRNV SCV ++++ RVYNVDDLKE
Sbjct: 347 TSTASETPLFLKEHVENLPQASPEVGGLRHFVDISVPRNVGSCVGEVETARVYNVDDLKE 406
Query: 774 VVAANKEDRLRKAMEAQTIIAEESKQFEAWRDSLETVPTIKKLRAYAERIRVAELDKCLG 595
VVAANKEDR+RKAMEAQTII EES QFEAWRDSLETVPTIKKLRAYAERIRVAEL+KC+
Sbjct: 407 VVAANKEDRMRKAMEAQTIITEESTQFEAWRDSLETVPTIKKLRAYAERIRVAELEKCMS 466
Query: 594 KMGEDISKKMRRAVDDLSRGMVNRLLHGPMQHLRCDGSDSRTLNETLENMHALNRMFSLE 415
KMG+DI+KK RAVDDLSRG+VNR LHGPMQHLRCDGSDSRTL+ETLENMHALNRM+ LE
Sbjct: 467 KMGDDINKKTTRAVDDLSRGIVNRFLHGPMQHLRCDGSDSRTLSETLENMHALNRMYGLE 526
Query: 414 TEISVLEQKIRAKVEQNQK 358
+I LE+K++A EQ K
Sbjct: 527 KDI--LEEKLKAMAEQQHK 543
>sp|P49294|HMA2_ARATH Glutamyl-tRNA reductase 2, chloroplast precursor (GluTR)
gi|2146736|pir||S65773 glutamyl-tRNA reductase (EC
1.2.1.-) precursor, chloroplast - Arabidopsis thaliana
gi|1049057|gb|AAB01674.1| glutamyl-tRNA reductase
Length = 530
Score = 467 bits (1202), Expect = e-130
Identities = 244/318 (76%), Positives = 276/318 (86%), Gaps = 3/318 (0%)
Frame = -1
Query: 1311 GFGRNISGLFKHAITVGKRVRAETNIAAGAVSVSSAAVELAFMKLPGTSH--DARMLVIG 1138
GFGRNISGLFKHAITVGKRVR ETNIAAGAVSVSSAAVELA MKLP +SH ARMLV+G
Sbjct: 218 GFGRNISGLFKHAITVGKRVRTETNIAAGAVSVSSAAVELALMKLPESSHASSARMLVVG 277
Query: 1137 AGKMGKLVIKHLVAKGCTKMVVVNRTEERVAAIREELKD-VEIIYKPLSEMLTCVGEADV 961
AGKMGKLVIKHLVAKGCTKMVVVNR+EE+VAA+R E+ VEIIYKPL EML+C EADV
Sbjct: 278 AGKMGKLVIKHLVAKGCTKMVVVNRSEEKVAAVRNEMPPGVEIIYKPLDEMLSCAAEADV 337
Query: 960 VFTSTASETPLFMKDHVKDLPPASQDVGGQRIFIDISVPRNVDSCVSDLDSVRVYNVDDL 781
VFTSTASETPLF+K+ V+ LPP V R+F+DISVPRNV SCV+++D RV+NVDDL
Sbjct: 338 VFTSTASETPLFLKEQVETLPP----VRDARLFVDISVPRNVGSCVAEIDGTRVFNVDDL 393
Query: 780 KEVVAANKEDRLRKAMEAQTIIAEESKQFEAWRDSLETVPTIKKLRAYAERIRVAELDKC 601
KEVVAANKEDR+RKAMEAQ II +ESK FEAWRDSLETVPTIKKLR Y ERI AE++K
Sbjct: 394 KEVVAANKEDRVRKAMEAQAIITDESKHFEAWRDSLETVPTIKKLRGYTERIIAAEIEKS 453
Query: 600 LGKMGEDISKKMRRAVDDLSRGMVNRLLHGPMQHLRCDGSDSRTLNETLENMHALNRMFS 421
L KMG D++KKMR+ VDDL RG+VN+LLHGPMQHLRCDG+DSRTL+ETL+NM ALNRM+
Sbjct: 454 LPKMGIDMNKKMRKTVDDLIRGIVNKLLHGPMQHLRCDGNDSRTLSETLDNMQALNRMYG 513
Query: 420 LETEISVLEQKIRAKVEQ 367
L+ EI LE+KIRAKVE+
Sbjct: 514 LDAEI--LEEKIRAKVEK 529
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,102,194,731
Number of Sequences: 1393205
Number of extensions: 24695964
Number of successful extensions: 63190
Number of sequences better than 10.0: 265
Number of HSP's better than 10.0 without gapping: 58279
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 62480
length of database: 448,689,247
effective HSP length: 126
effective length of database: 273,145,417
effective search space used: 84675079270
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
