Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003203A_C01 KMC003203A_c01
(482 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAL07089.1| putative nonsense-mediated mRNA decay protein [Ar... 121 3e-27
ref|NP_178476.1| putative nonsense-mediated mRNA decay protein; ... 121 3e-27
gb|AAK00432.1|AC060755_2 hypothetical protein [Oryza sativa] 77 1e-13
gb|EAA11292.1| agCP5858 [Anopheles gambiae str. PEST] 58 6e-08
ref|NP_593077.1| putative nonsense-mediated mrna decay protein [... 57 1e-07
>gb|AAL07089.1| putative nonsense-mediated mRNA decay protein [Arabidopsis
thaliana]
Length = 516
Score = 121 bits (304), Expect(2) = 3e-27
Identities = 57/80 (71%), Positives = 68/80 (84%)
Frame = -2
Query: 478 KARSWKLKSLNMEVDDKVARADPDKMHSEYEQFLKDLEENPDLRFNISLYRNKEYQPSEI 299
K+R+WKLKSL ME+DD R DP+K EYE+FL+DLEENP+LRFNISLYR+K+YQ SE
Sbjct: 418 KSRNWKLKSLPMEMDDSRGRVDPEKTDKEYEEFLRDLEENPELRFNISLYRDKDYQASET 477
Query: 298 ASVTDGEELPSVPLEELLAD 239
AS+TDGE PSVP+EELLAD
Sbjct: 478 ASMTDGEGAPSVPIEELLAD 497
Score = 21.2 bits (43), Expect(2) = 3e-27
Identities = 7/9 (77%), Positives = 9/9 (99%)
Frame = -3
Query: 225 EDEDEEDDM 199
ED+D+EDDM
Sbjct: 505 EDDDDEDDM 513
>ref|NP_178476.1| putative nonsense-mediated mRNA decay protein; protein id:
At2g03820.1, supported by cDNA: gi_15810402, supported
by cDNA: gi_20466623 [Arabidopsis thaliana]
gi|25350038|pir||F84452 probable nonsense-mediated mRNA
decay protein [imported] - Arabidopsis thaliana
gi|4582430|gb|AAD24816.1| putative nonsense-mediated
mRNA decay protein [Arabidopsis thaliana]
gi|20466624|gb|AAM20629.1| putative nonsense-mediated
mRNA decay protein [Arabidopsis thaliana]
gi|22136400|gb|AAM91278.1| putative nonsense-mediated
mRNA decay protein [Arabidopsis thaliana]
Length = 516
Score = 121 bits (304), Expect(2) = 3e-27
Identities = 57/80 (71%), Positives = 68/80 (84%)
Frame = -2
Query: 478 KARSWKLKSLNMEVDDKVARADPDKMHSEYEQFLKDLEENPDLRFNISLYRNKEYQPSEI 299
K+R+WKLKSL ME+DD R DP+K EYE+FL+DLEENP+LRFNISLYR+K+YQ SE
Sbjct: 418 KSRNWKLKSLPMEMDDSRGRVDPEKTDKEYEEFLRDLEENPELRFNISLYRDKDYQASET 477
Query: 298 ASVTDGEELPSVPLEELLAD 239
AS+TDGE PSVP+EELLAD
Sbjct: 478 ASMTDGEGAPSVPIEELLAD 497
Score = 21.2 bits (43), Expect(2) = 3e-27
Identities = 7/9 (77%), Positives = 9/9 (99%)
Frame = -3
Query: 225 EDEDEEDDM 199
ED+D+EDDM
Sbjct: 505 EDDDDEDDM 513
>gb|AAK00432.1|AC060755_2 hypothetical protein [Oryza sativa]
Length = 711
Score = 76.6 bits (187), Expect = 1e-13
Identities = 40/81 (49%), Positives = 59/81 (72%), Gaps = 1/81 (1%)
Frame = -2
Query: 478 KARSWKLKSLNMEVDD-KVARADPDKMHSEYEQFLKDLEENPDLRFNISLYRNKEYQPSE 302
+ R WKLK L ME D A+ + +K EYE+FL+DLE N D+RF I LY++++Y+ SE
Sbjct: 610 RTRRWKLKRLPMEEDAVNKAKGELEKRADEYEEFLRDLEHNSDIRFQIDLYKDEDYR-SE 668
Query: 301 IASVTDGEELPSVPLEELLAD 239
+AS T G+++P+VP+EEL+ D
Sbjct: 669 MAS-TIGDDVPTVPIEELIED 688
>gb|EAA11292.1| agCP5858 [Anopheles gambiae str. PEST]
Length = 523
Score = 57.8 bits (138), Expect = 6e-08
Identities = 31/81 (38%), Positives = 52/81 (63%), Gaps = 1/81 (1%)
Frame = -2
Query: 478 KARSWKLKSLNMEVDDKVARADPDKMHSEYEQFLKDLEENPDLRFNISLYRNKEYQPSEI 299
++R+WKLK LN E+ D D + +EY +FL+DLEE+P+LR N++++++K Q +
Sbjct: 437 QSRNWKLKHLNEEIV-----VDTD-IENEYNEFLEDLEEDPELRQNVNIFKDKNKQQIPV 490
Query: 298 -ASVTDGEELPSVPLEELLAD 239
+ D +P + LEE+L D
Sbjct: 491 DTNDMDDPSVPRITLEEMLDD 511
>ref|NP_593077.1| putative nonsense-mediated mrna decay protein [Schizosaccharomyces
pombe] gi|1175492|sp|Q09817|YAC3_SCHPO Hypothetical
protein C16C9.03 in chromosome I gi|2130280|pir||S62473
probable nonsense-mediated mrna decay protein - fission
yeast (Schizosaccharomyces pombe)
gi|1019815|emb|CAA91191.1| putative nonsense-mediated
mrna decay protein [Schizosaccharomyces pombe]
Length = 498
Score = 56.6 bits (135), Expect = 1e-07
Identities = 29/89 (32%), Positives = 53/89 (58%), Gaps = 9/89 (10%)
Frame = -2
Query: 478 KARSWKLKSLNMEVDDKVARADPDKMHSEYEQFLKDLEENPDLRFNISLYRNKEYQPSEI 299
K R+W+LK++ M+ + V + D ++ +YE FL++LEE+P+LR ++LY+ P +
Sbjct: 408 KNRNWRLKTIGMQKAEDVKKQDIERQERDYELFLQNLEEDPELRQGVNLYK----APVKA 463
Query: 298 ASVTD---------GEELPSVPLEELLAD 239
+V D E++P + ++ELL D
Sbjct: 464 IAVADTDMDEEDEVDEDIPQISVDELLDD 492
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 404,494,873
Number of Sequences: 1393205
Number of extensions: 8587625
Number of successful extensions: 51616
Number of sequences better than 10.0: 73
Number of HSP's better than 10.0 without gapping: 34326
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 44771
length of database: 448,689,247
effective HSP length: 113
effective length of database: 291,257,082
effective search space used: 13689082854
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
