Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003082A_C01 KMC003082A_c01
(572 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAM23265.1| L-asparaginase [Glycine max] 315 3e-85
ref|NP_566536.1| putative L-asparaginase; protein id: At3g16150.... 298 4e-80
emb|CAC09349.1| putative l-asparaginase [Oryza sativa (indica cu... 272 2e-72
gb|AAG28786.1|AF308474_1 asparaginase [Hordeum vulgare] 269 2e-71
ref|NP_196427.1| asparaginase; protein id: At5g08100.1, supporte... 197 1e-49
>gb|AAM23265.1| L-asparaginase [Glycine max]
Length = 326
Score = 315 bits (806), Expect = 3e-85
Identities = 155/174 (89%), Positives = 164/174 (94%)
Frame = +1
Query: 49 MGRWAIAVHGGAGVDPNLPLQRQEEAKQLLTRCLNLGISALTSNLSAIDVVELVVRELET 228
MG WAIAVHGGAGVDPNLPL+ QEEAKQLLTRCLNLGISAL SNL AIDVVELVVRELET
Sbjct: 1 MGGWAIAVHGGAGVDPNLPLEHQEEAKQLLTRCLNLGISALNSNLPAIDVVELVVRELET 60
Query: 229 DPIFNSGRGSALTEKGTVEMEASIMDGPKRRCGAVAGVTTVKNPISLARWVMDKSPHSYL 408
DP+FNSGRGSALTEKGTVEMEASIMDGPKRRCGAV+G+TTVKNPISLAR VMDKSPHSYL
Sbjct: 61 DPLFNSGRGSALTEKGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
Query: 409 AFDGAEDFARKQGVELVDNEYFITPDKVGMLKLAKEAKAILFDYRVPTTAYETC 570
AF GAE+FAR+QGVE+VDNEYFITPD VGMLKLAKEA ILFDYR+PT+ YETC
Sbjct: 121 AFSGAEEFARQQGVEVVDNEYFITPDNVGMLKLAKEANTILFDYRIPTSGYETC 174
>ref|NP_566536.1| putative L-asparaginase; protein id: At3g16150.1, supported by
cDNA: 21689. [Arabidopsis thaliana]
gi|9294462|dbj|BAB02681.1| l-asparaginase (l-asparagine
amidohydrolase) [Arabidopsis thaliana]
gi|21554260|gb|AAM63335.1| putative L-asparaginase
[Arabidopsis thaliana]
Length = 325
Score = 298 bits (762), Expect = 4e-80
Identities = 146/167 (87%), Positives = 157/167 (93%)
Frame = +1
Query: 49 MGRWAIAVHGGAGVDPNLPLQRQEEAKQLLTRCLNLGISALTSNLSAIDVVELVVRELET 228
MG WAIAVHGGAG+DPNLP +RQEEAKQLLTRCLNLGI AL SN+SAIDVVELV+RELET
Sbjct: 1 MGGWAIAVHGGAGIDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELET 60
Query: 229 DPIFNSGRGSALTEKGTVEMEASIMDGPKRRCGAVAGVTTVKNPISLARWVMDKSPHSYL 408
DP+FNSGRGSALTEKGTVEMEASIMDG KRRCGAV+G+TTVKNPISLAR VMDKSPHSYL
Sbjct: 61 DPLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120
Query: 409 AFDGAEDFARKQGVELVDNEYFITPDKVGMLKLAKEAKAILFDYRVP 549
AF GAEDFARKQGVE+VDNEYF+T D VGMLKLAKEA +ILFDYR+P
Sbjct: 121 AFSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIP 167
>emb|CAC09349.1| putative l-asparaginase [Oryza sativa (indica cultivar-group)]
Length = 333
Score = 272 bits (695), Expect = 2e-72
Identities = 133/174 (76%), Positives = 149/174 (85%)
Frame = +1
Query: 49 MGRWAIAVHGGAGVDPNLPLQRQEEAKQLLTRCLNLGISALTSNLSAIDVVELVVRELET 228
M RWAIA+HGGAGVDPNLP RQEEAK++L RCL +G+ AL S +A+DVVE VVRELE+
Sbjct: 1 MARWAIAIHGGAGVDPNLPEHRQEEAKRVLARCLQVGVDALRSGAAALDVVEAVVRELES 60
Query: 229 DPIFNSGRGSALTEKGTVEMEASIMDGPKRRCGAVAGVTTVKNPISLARWVMDKSPHSYL 408
DP FNSGRGSALT GTVEMEASIMDG RRCGAV+GV+TVKNP+SLAR VMDKSPHSYL
Sbjct: 61 DPFFNSGRGSALTRLGTVEMEASIMDGRGRRCGAVSGVSTVKNPVSLARLVMDKSPHSYL 120
Query: 409 AFDGAEDFARKQGVELVDNEYFITPDKVGMLKLAKEAKAILFDYRVPTTAYETC 570
AFDGAE FAR QG+E+VDN YFIT D VGMLKLAKEA +ILFDYR+P + ETC
Sbjct: 121 AFDGAEQFARDQGLEVVDNSYFITEDNVGMLKLAKEANSILFDYRIPLSGSETC 174
>gb|AAG28786.1|AF308474_1 asparaginase [Hordeum vulgare]
Length = 333
Score = 269 bits (687), Expect = 2e-71
Identities = 130/174 (74%), Positives = 149/174 (84%)
Frame = +1
Query: 49 MGRWAIAVHGGAGVDPNLPLQRQEEAKQLLTRCLNLGISALTSNLSAIDVVELVVRELET 228
M RWAIA+HGGAGVDPNLP RQEEAK++L RCL +G+ L + +A+DVVE VVRELET
Sbjct: 1 MARWAIAIHGGAGVDPNLPEHRQEEAKRVLARCLQVGVDLLRAGATALDVVEAVVRELET 60
Query: 229 DPIFNSGRGSALTEKGTVEMEASIMDGPKRRCGAVAGVTTVKNPISLARWVMDKSPHSYL 408
DP FNSGRGSALT GTVEMEASIMDG RRCGAV+GV+TVKNP+SLAR VMD+SPHSYL
Sbjct: 61 DPCFNSGRGSALTRAGTVEMEASIMDGRGRRCGAVSGVSTVKNPVSLARRVMDRSPHSYL 120
Query: 409 AFDGAEDFARKQGVELVDNEYFITPDKVGMLKLAKEAKAILFDYRVPTTAYETC 570
AFDGAEDFAR+QG+E+VDN YFIT + VGMLKLAKEA +ILFDYR+P +TC
Sbjct: 121 AFDGAEDFAREQGLEVVDNSYFITEENVGMLKLAKEANSILFDYRIPLAGADTC 174
>ref|NP_196427.1| asparaginase; protein id: At5g08100.1, supported by cDNA:
gi_14517439, supported by cDNA: gi_20147330 [Arabidopsis
thaliana] gi|25108880|sp|P50287|ASPG_ARATH
L-asparaginase (L-asparagine amidohydrolase)
gi|11280080|pir||T50495 asparaginase - Arabidopsis
thaliana gi|8346547|emb|CAB93711.1| asparaginase
[Arabidopsis thaliana] gi|14517440|gb|AAK62610.1|
AT5g08100/T22D6_40 [Arabidopsis thaliana]
gi|20147331|gb|AAM10379.1| AT5g08100/T22D6_40
[Arabidopsis thaliana]
Length = 315
Score = 197 bits (500), Expect = 1e-49
Identities = 102/166 (61%), Positives = 123/166 (73%), Gaps = 1/166 (0%)
Frame = +1
Query: 58 WAIAVHGGAGVDP-NLPLQRQEEAKQLLTRCLNLGISALTSNLSAIDVVELVVRELETDP 234
WAIA+HGGAG P +LP +R+ + L CL+LGISAL S +DV ELVVRELE P
Sbjct: 4 WAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELENHP 63
Query: 235 IFNSGRGSALTEKGTVEMEASIMDGPKRRCGAVAGVTTVKNPISLARWVMDKSPHSYLAF 414
FN+G+GS LT +GTVEMEASIMDG +RCGAV+G+TTV NPISLAR VM+K+PH YLAF
Sbjct: 64 DFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAF 123
Query: 415 DGAEDFARKQGVELVDNEYFITPDKVGMLKLAKEAKAILFDYRVPT 552
D AE FAR GVE VD+ +FITP+ + LK AKE + DY VP+
Sbjct: 124 DAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPS 169
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.316 0.133 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 569,767,797
Number of Sequences: 1393205
Number of extensions: 13776284
Number of successful extensions: 61272
Number of sequences better than 10.0: 177
Number of HSP's better than 10.0 without gapping: 49048
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 58090
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 21243732558
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
