Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003034A_C01 KMC003034A_c01
(334 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAA50755.1| cysteine proteinase 60 1e-11
gb|AAB23155.1| COT44=cysteine proteinase homolog [Brassica napus... 64 2e-11
sp|P25251|CYS4_BRANA Cysteine proteinase COT44 precursor gi|8168... 64 2e-11
gb|AAL14199.1| cysteine proteinase precursor [Ipomoea batatas] 58 4e-11
gb|AAK27968.1|AF242372_1 cysteine protease [Ipomoea batatas] 58 4e-11
>gb|AAA50755.1| cysteine proteinase
Length = 340
Score = 59.7 bits (143), Expect(2) = 1e-11
Identities = 25/37 (67%), Positives = 31/37 (83%), Gaps = 1/37 (2%)
Frame = -1
Query: 334 YGRAN-GTDYWIVKNSWGTVWGEQGYIRMQRGIAAKD 227
YG ++ GT YW+VKNSWGT WGE+GYIRMQR + AK+
Sbjct: 290 YGTSDDGTKYWLVKNSWGTQWGEEGYIRMQRDVDAKE 326
Score = 30.0 bits (66), Expect(2) = 1e-11
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = -2
Query: 231 RTGLCGIAMDSSYPTA 184
+ GLCGIAM +SYPTA
Sbjct: 325 KEGLCGIAMKASYPTA 340
>gb|AAB23155.1| COT44=cysteine proteinase homolog [Brassica napus, seedling, rapid
cycling base population CrGC5, Peptide, 328 aa]
Length = 328
Score = 64.3 bits (155), Expect(2) = 2e-11
Identities = 25/35 (71%), Positives = 29/35 (82%)
Frame = -1
Query: 334 YGRANGTDYWIVKNSWGTVWGEQGYIRMQRGIAAK 230
YG NG DYWIV+NSWGT WGE GYIRM+R +A+K
Sbjct: 267 YGSENGVDYWIVRNSWGTRWGEDGYIRMERNVASK 301
Score = 24.6 bits (52), Expect(2) = 2e-11
Identities = 8/14 (57%), Positives = 13/14 (92%)
Frame = -2
Query: 231 RTGLCGIAMDSSYP 190
++G CGIA+++SYP
Sbjct: 301 KSGKCGIAIEASYP 314
>sp|P25251|CYS4_BRANA Cysteine proteinase COT44 precursor gi|81682|pir||JQ1121 cysteine
proteinase (EC 3.4.22.-) COT44 [similarity] - rape
Length = 328
Score = 64.3 bits (155), Expect(2) = 2e-11
Identities = 25/35 (71%), Positives = 29/35 (82%)
Frame = -1
Query: 334 YGRANGTDYWIVKNSWGTVWGEQGYIRMQRGIAAK 230
YG NG DYWIV+NSWGT WGE GYIRM+R +A+K
Sbjct: 267 YGSENGVDYWIVRNSWGTRWGEDGYIRMERNVASK 301
Score = 24.6 bits (52), Expect(2) = 2e-11
Identities = 8/14 (57%), Positives = 13/14 (92%)
Frame = -2
Query: 231 RTGLCGIAMDSSYP 190
++G CGIA+++SYP
Sbjct: 301 KSGKCGIAIEASYP 314
>gb|AAL14199.1| cysteine proteinase precursor [Ipomoea batatas]
Length = 341
Score = 57.8 bits (138), Expect(2) = 4e-11
Identities = 25/37 (67%), Positives = 31/37 (83%), Gaps = 1/37 (2%)
Frame = -1
Query: 334 YGRA-NGTDYWIVKNSWGTVWGEQGYIRMQRGIAAKD 227
YG A +G+ YW+VKNSWGT WGE+GYIRMQ+ I AK+
Sbjct: 291 YGIAEDGSKYWLVKNSWGTSWGEKGYIRMQKDIEAKE 327
Score = 30.0 bits (66), Expect(2) = 4e-11
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = -2
Query: 231 RTGLCGIAMDSSYPTA 184
+ GLCGIAM SSYP+A
Sbjct: 326 KEGLCGIAMQSSYPSA 341
>gb|AAK27968.1|AF242372_1 cysteine protease [Ipomoea batatas]
Length = 339
Score = 57.8 bits (138), Expect(2) = 4e-11
Identities = 25/37 (67%), Positives = 31/37 (83%), Gaps = 1/37 (2%)
Frame = -1
Query: 334 YGRA-NGTDYWIVKNSWGTVWGEQGYIRMQRGIAAKD 227
YG A +G+ YW+VKNSWGT WGE+GYIRMQ+ I AK+
Sbjct: 289 YGIAEDGSKYWLVKNSWGTSWGEKGYIRMQKDIEAKE 325
Score = 30.0 bits (66), Expect(2) = 4e-11
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = -2
Query: 231 RTGLCGIAMDSSYPTA 184
+ GLCGIAM SSYP+A
Sbjct: 324 KEGLCGIAMQSSYPSA 339
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 281,853,130
Number of Sequences: 1393205
Number of extensions: 5215871
Number of successful extensions: 12003
Number of sequences better than 10.0: 970
Number of HSP's better than 10.0 without gapping: 11557
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 11989
length of database: 448,689,247
effective HSP length: 86
effective length of database: 328,873,617
effective search space used: 7892966808
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)