KMC002951A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC002951A_C01 KMC002951A_c01
ttaatgagactacatatattaattgttctttgtgtgaaacctATTTTTACTTTTAGTTCA
TCGTAATTCTACATAGAATTACTTCAGAAATATGACATAAATAGCATCGCAGTACCAAGC
AAACAGCGGAGCATAGTAAACTTAATTCTAGCTGGAAGCCAGTGTTACAAGGCATTATAT
GAAAGAATCTGTAAACCCCCCAAAACTGAAGACACATCAGAACAAAGTTGTGGCATTCAC
AAAGCTGTTTAGCTAGCACTGTGTGTGTATATATTTAAGTAAAGTTTTTCGTCAGAAAAA
GAGCCTATTTGAACCTTCACTTTTCAATTCGTTCAACCTTGAGGAAGTAAGCAGCTACTG
CAAGTAGGATGATAAATAAGGCAATCCACAAGCTCACCCCTCCACTTGTCTTCTTCCCTT
CAATTATGGTTACCCTAGCACTTGAATCCATGGATACAGAAGCCAAAGCCCTTGAATCAG
GGGAGAATGCCAAAGCAGTTACTATGCCAAGGTGAGCCTTCTTTATCGTTGTCTGAATCT
GCATATTTGTTGAACTTACAATTACAATGTCTCCTGAAGGTGTCCACATGCAAGAA


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002951A_C01 KMC002951A_c01
         (596 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_178256.1| putative protein transport protein SEC12p; prot...    88  9e-17
pir||T48907 St12p protein [imported] - Arabidopsis thaliana gi|1...    87  1e-16
ref|NP_568738.1| St12p protein; protein id: At5g50550.1 [Arabido...    87  2e-16
pir||S28604 St12p protein - Arabidopsis thaliana                       74  1e-12
dbj|BAA96978.1| protein transport protein SEC12p-like [Arabidops...    73  2e-12

>ref|NP_178256.1| putative protein transport protein SEC12p; protein id: At2g01470.1,
           supported by cDNA: gi_166877, supported by cDNA:
           gi_18377756 [Arabidopsis thaliana]
           gi|25410908|pir||B84425 probable protein transport
           protein SEC12p [imported] - Arabidopsis thaliana
           gi|3785976|gb|AAC67323.1| putative protein transport
           protein SEC12p [Arabidopsis thaliana]
           gi|18377757|gb|AAL67028.1| putative protein transport
           protein SEC12p [Arabidopsis thaliana]
           gi|21689839|gb|AAM67563.1| putative protein transport
           protein SEC12p [Arabidopsis thaliana]
          Length = 393

 Score = 87.8 bits (216), Expect = 9e-17
 Identities = 46/88 (52%), Positives = 59/88 (66%), Gaps = 2/88 (2%)
 Frame = -2

Query: 583 TPSGDIVIVSSTNMQIQTTIKKAHLGIVTALAFSPDSRALASVSMDSSARVTIIEGKKTS 404
           T  GD++I+ ST MQ    +KKAHLG+VTAL FSPDSR L SVS DS AR+T+IE K   
Sbjct: 304 TLEGDVLILESTRMQTIQVVKKAHLGLVTALTFSPDSRGLVSVSFDSRARLTMIEQKGDK 363

Query: 403 GGVSLW--IALFIILLAVAAYFLKVERI 326
            GV  W  + L ++L  VA Y++K + I
Sbjct: 364 PGVRWWLLVLLIVLLYVVAYYYMKAKGI 391

>pir||T48907 St12p protein [imported] - Arabidopsis thaliana
           gi|166878|gb|AAA32871.1| St12p protein
          Length = 393

 Score = 87.4 bits (215), Expect = 1e-16
 Identities = 46/88 (52%), Positives = 59/88 (66%), Gaps = 2/88 (2%)
 Frame = -2

Query: 583 TPSGDIVIVSSTNMQIQTTIKKAHLGIVTALAFSPDSRALASVSMDSSARVTIIEGKKTS 404
           T  GD++I+ ST MQ    +KKAHLG+VTAL FSPDSR L SVS DS AR+T+IE K   
Sbjct: 304 TLEGDVLILESTRMQTIQIVKKAHLGLVTALTFSPDSRGLVSVSFDSRARLTMIEQKGDK 363

Query: 403 GGVSLW--IALFIILLAVAAYFLKVERI 326
            GV  W  + L ++L  VA Y++K + I
Sbjct: 364 PGVRWWLLVLLIVLLYVVAYYYMKAKGI 391

>ref|NP_568738.1| St12p protein; protein id: At5g50550.1 [Arabidopsis thaliana]
           gi|22327692|ref|NP_680414.1| St12p protein; protein id:
           At5g50650.1 [Arabidopsis thaliana]
           gi|9758764|dbj|BAB09140.1| St12p protein [Arabidopsis
           thaliana]
          Length = 383

 Score = 86.7 bits (213), Expect = 2e-16
 Identities = 45/84 (53%), Positives = 57/84 (67%), Gaps = 1/84 (1%)
 Frame = -2

Query: 583 TPSGDIVIVSSTNMQIQTTIKKAHLGIVTALAFSPDSRALASVSMDSSARVTIIEGKKTS 404
           T  GD++I+ ST MQ    +KKAHLG+VTAL FSPDSR L SVS DS AR+T+I+ K   
Sbjct: 294 TLEGDVLIIDSTKMQTNQIVKKAHLGLVTALTFSPDSRCLVSVSFDSRARLTVIKQKGEK 353

Query: 403 GGVSLWI-ALFIILLAVAAYFLKV 335
             V LW+ AL  +L+ V  Y+L V
Sbjct: 354 RRVYLWVAALLFVLVYVVLYYLMV 377

>pir||S28604 St12p protein - Arabidopsis thaliana
          Length = 367

 Score = 73.9 bits (180), Expect = 1e-12
 Identities = 38/63 (60%), Positives = 45/63 (71%)
 Frame = -2

Query: 583 TPSGDIVIVSSTNMQIQTTIKKAHLGIVTALAFSPDSRALASVSMDSSARVTIIEGKKTS 404
           T  GD++I+ ST MQ    +KKAHLG+VTAL FSPDSR L SVS DS AR+T+IE K   
Sbjct: 304 TLEGDVLILESTRMQTIQIVKKAHLGLVTALTFSPDSRGLVSVSFDSRARLTMIEQKGDK 363

Query: 403 GGV 395
            GV
Sbjct: 364 PGV 366

>dbj|BAA96978.1| protein transport protein SEC12p-like [Arabidopsis thaliana]
          Length = 372

 Score = 73.2 bits (178), Expect = 2e-12
 Identities = 35/57 (61%), Positives = 43/57 (75%)
 Frame = -2

Query: 583 TPSGDIVIVSSTNMQIQTTIKKAHLGIVTALAFSPDSRALASVSMDSSARVTIIEGK 413
           T  GD++I+ ST MQ    +KKAHLG+VTAL FSPDSR L SVS DS AR+T+I+ K
Sbjct: 294 TLEGDVLIIDSTKMQTNQIVKKAHLGLVTALTFSPDSRCLVSVSFDSRARLTVIKQK 350

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 478,961,062
Number of Sequences: 1393205
Number of extensions: 9779184
Number of successful extensions: 23267
Number of sequences better than 10.0: 94
Number of HSP's better than 10.0 without gapping: 21655
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23260
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 23140425222
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 SPD036f11_f BP046887 1 571
2 MR074f07_f BP081706 43 407
3 MR007e06_f BP076485 55 420
4 MWM050g05_f AV765480 55 572
5 MR011d02_f BP076780 72 407
6 GNf008d07 BP067951 83 544
7 SPDL007g12_f BP052439 85 561
8 SPD091c11_f BP051262 88 226
9 MPD072d04_f AV774719 111 543
10 MFB082d02_f BP039996 133 599
11 SPD036f01_f BP046879 263 569




Lotus japonicus
Kazusa DNA Research Institute