Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC002946A_C02 KMC002946A_c02
(627 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAC97389.1| 4-coumarate:CoA ligase isoenzyme 3 [Glycine max] ... 134 4e-35
sp|P31687|4CL2_SOYBN 4-coumarate--CoA ligase 2 (4CL 2) (4-coumar... 134 4e-35
pir||PQ0772 4-coumarate-CoA ligase (EC 6.2.1.12) (clone GM4CL1B)... 134 4e-35
pir||PQ0773 4-coumarate-CoA ligase (EC 6.2.1.12) (clone GM4CL13)... 134 4e-35
dbj|BAA08366.2| 4-coumarate:CoA ligase [Lithospermum erythrorhizon] 125 4e-32
>gb|AAC97389.1| 4-coumarate:CoA ligase isoenzyme 3 [Glycine max]
gi|4038973|gb|AAC97599.1| 4-coumarate:CoA ligase
isoenzyme 3 [Glycine max]
Length = 570
Score = 134 bits (337), Expect(2) = 4e-35
Identities = 66/73 (90%), Positives = 71/73 (96%)
Frame = -3
Query: 625 IKFKGFQVPPAELEGLLVSHPSIADAAVVPRKDAAAGEVPVAFVVRSNGFDLTEEAVKEF 446
IK+KGFQVPPAELEGLLVSHPSIADAAVVP+KD AAGEVPVAFVVRSNGFDLTEEAVKEF
Sbjct: 466 IKYKGFQVPPAELEGLLVSHPSIADAAVVPQKDVAAGEVPVAFVVRSNGFDLTEEAVKEF 525
Query: 445 ISQPVVFYKRLHQ 407
I++ VVFYKRLH+
Sbjct: 526 IAKQVVFYKRLHK 538
Score = 35.8 bits (81), Expect(2) = 4e-35
Identities = 16/21 (76%), Positives = 17/21 (80%)
Frame = -1
Query: 426 FIKDCTKVYFVHAIPKSPSGQ 364
F K KVYFVHAIPKSPSG+
Sbjct: 532 FYKRLHKVYFVHAIPKSPSGK 552
>sp|P31687|4CL2_SOYBN 4-coumarate--CoA ligase 2 (4CL 2) (4-coumaroyl-CoA synthase 2)
(Clone 4CL16) gi|13559169|emb|CAC36095.1|
4-coumarate:Coenzyme A ligase isoenzyme 4 [Glycine max]
Length = 562
Score = 134 bits (337), Expect(2) = 4e-35
Identities = 66/73 (90%), Positives = 71/73 (96%)
Frame = -3
Query: 625 IKFKGFQVPPAELEGLLVSHPSIADAAVVPRKDAAAGEVPVAFVVRSNGFDLTEEAVKEF 446
IK+KGFQVPPAELEGLLVSHPSIADAAVVP+KD AAGEVPVAFVVRSNGFDLTEEAVKEF
Sbjct: 458 IKYKGFQVPPAELEGLLVSHPSIADAAVVPQKDVAAGEVPVAFVVRSNGFDLTEEAVKEF 517
Query: 445 ISQPVVFYKRLHQ 407
I++ VVFYKRLH+
Sbjct: 518 IAKQVVFYKRLHK 530
Score = 35.8 bits (81), Expect(2) = 4e-35
Identities = 16/21 (76%), Positives = 17/21 (80%)
Frame = -1
Query: 426 FIKDCTKVYFVHAIPKSPSGQ 364
F K KVYFVHAIPKSPSG+
Sbjct: 524 FYKRLHKVYFVHAIPKSPSGK 544
>pir||PQ0772 4-coumarate-CoA ligase (EC 6.2.1.12) (clone GM4CL1B) - soybean
(fragment)
Length = 423
Score = 134 bits (337), Expect(2) = 4e-35
Identities = 66/73 (90%), Positives = 71/73 (96%)
Frame = -3
Query: 625 IKFKGFQVPPAELEGLLVSHPSIADAAVVPRKDAAAGEVPVAFVVRSNGFDLTEEAVKEF 446
IK+KGFQVPPAELEGLLVSHPSIADAAVVP+KD AAGEVPVAFVVRSNGFDLTEEAVKEF
Sbjct: 319 IKYKGFQVPPAELEGLLVSHPSIADAAVVPQKDVAAGEVPVAFVVRSNGFDLTEEAVKEF 378
Query: 445 ISQPVVFYKRLHQ 407
I++ VVFYKRLH+
Sbjct: 379 IAKQVVFYKRLHK 391
Score = 35.8 bits (81), Expect(2) = 4e-35
Identities = 16/21 (76%), Positives = 17/21 (80%)
Frame = -1
Query: 426 FIKDCTKVYFVHAIPKSPSGQ 364
F K KVYFVHAIPKSPSG+
Sbjct: 385 FYKRLHKVYFVHAIPKSPSGK 405
>pir||PQ0773 4-coumarate-CoA ligase (EC 6.2.1.12) (clone GM4CL13) - soybean
(fragment)
Length = 235
Score = 134 bits (337), Expect(2) = 4e-35
Identities = 66/73 (90%), Positives = 71/73 (96%)
Frame = -3
Query: 625 IKFKGFQVPPAELEGLLVSHPSIADAAVVPRKDAAAGEVPVAFVVRSNGFDLTEEAVKEF 446
IK+KGFQVPPAELEGLLVSHPSIADAAVVP+KD AAGEVPVAFVVRSNGFDLTEEAVKEF
Sbjct: 131 IKYKGFQVPPAELEGLLVSHPSIADAAVVPQKDVAAGEVPVAFVVRSNGFDLTEEAVKEF 190
Query: 445 ISQPVVFYKRLHQ 407
I++ VVFYKRLH+
Sbjct: 191 IAKQVVFYKRLHK 203
Score = 35.8 bits (81), Expect(2) = 4e-35
Identities = 16/21 (76%), Positives = 17/21 (80%)
Frame = -1
Query: 426 FIKDCTKVYFVHAIPKSPSGQ 364
F K KVYFVHAIPKSPSG+
Sbjct: 197 FYKRLHKVYFVHAIPKSPSGK 217
>dbj|BAA08366.2| 4-coumarate:CoA ligase [Lithospermum erythrorhizon]
Length = 585
Score = 125 bits (314), Expect(2) = 4e-32
Identities = 64/74 (86%), Positives = 70/74 (94%), Gaps = 1/74 (1%)
Frame = -3
Query: 625 IKFKGFQVPPAELEGLLVSHPSIADAAVVPRKDAAAGEVPVAFVVRSN-GFDLTEEAVKE 449
IKFKGFQVPPAELE LL+SHP+IADAAVVP+KDAAAGEVPVAFVV SN GF+LTEEAVKE
Sbjct: 482 IKFKGFQVPPAELEALLISHPNIADAAVVPQKDAAAGEVPVAFVVPSNDGFELTEEAVKE 541
Query: 448 FISQPVVFYKRLHQ 407
FIS+ VVFYKRLH+
Sbjct: 542 FISKQVVFYKRLHK 555
Score = 34.7 bits (78), Expect(2) = 4e-32
Identities = 15/21 (71%), Positives = 17/21 (80%)
Frame = -1
Query: 426 FIKDCTKVYFVHAIPKSPSGQ 364
F K KVYFVH+IPKSPSG+
Sbjct: 549 FYKRLHKVYFVHSIPKSPSGK 569
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 537,796,206
Number of Sequences: 1393205
Number of extensions: 11748873
Number of successful extensions: 36280
Number of sequences better than 10.0: 1122
Number of HSP's better than 10.0 without gapping: 32982
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 36047
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 25586195130
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
