KMC002833A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC002833A_C01 KMC002833A_c01
atAAAGCAAAAGTAATACATTACTTCATTTATTGTAGCTACAAAAAATTACAAGCCATGT
CAGTCCTCATAAGTGACTGGCACAACTTTATGCATACAAAAAAAAAGATTAGCTGGATAG
GGACGGCTAAAATATTCATTCGACTTCAAGAGCAGACTGCTGCCTAAACTGAACACCGTG
AATGGACTATAAGTATCCTGAACCATTTCCACCCCCTCCAGGGAGGGGGAAAATGAAGAC
AAAGCAAAGGGATCCCTAATCTCACAAAAAGATAAATTCGATCCCACAAAAAAATTCTGT
CGCAGGAACTAATATCGAATATACTAAACGGCTTTCTTTCACCATCATGCATTAACTGAC
ACGGCTAGGCTACTAATCCAAGAAGATAAAGAGGAATGGGATAGACTCACCTAAAACCAT
CCAGGAACAACGCGTCAACCAGAACGCCGCTTAGATTCATTCCGCTTCCCTAGAAGTTTC
AGCATGTGGTCAGTGACATCAGGAGGAGATTCATCATCTTC


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002833A_C01 KMC002833A_c01
         (521 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_680142.1| similar to putative RNA helicase A; protein id:...    37  0.14
pir||T06053 probable ubiquitin-dependent proteolytic protein - A...    32  3.4
ref|NP_660506.1| NADH dehydrogenase I chain C, chain D [Buchnera...    32  4.5
ref|XP_233803.1| similar to ribosomal protein L7a, cytosolic [va...    31  7.6
ref|NP_181689.1| putative non-LTR retroelement reverse transcrip...    31  9.9

>ref|NP_680142.1| similar to putative RNA helicase A; protein id: At5g04895.1
           [Arabidopsis thaliana]
          Length = 581

 Score = 37.0 bits (84), Expect = 0.14
 Identities = 19/27 (70%), Positives = 22/27 (81%)
 Frame = -1

Query: 521 EDDESPPDVTDHMLKLLGKRNESKRRS 441
           ED +SPPDVTD+MLKLLG R   +RRS
Sbjct: 555 EDADSPPDVTDNMLKLLGGR---RRRS 578

>pir||T06053 probable ubiquitin-dependent proteolytic protein - Arabidopsis
           thaliana gi|4539312|emb|CAB38813.1| putative
           ubiquitin-dependent proteolytic protein [Arabidopsis
           thaliana] gi|7270876|emb|CAB80556.1| putative
           ubiquitin-dependent proteolytic protein [Arabidopsis
           thaliana]
          Length = 315

 Score = 32.3 bits (72), Expect = 3.4
 Identities = 16/39 (41%), Positives = 22/39 (56%), Gaps = 1/39 (2%)
 Frame = +2

Query: 140 STSRADCCLN*TP*MDYKYPE-PFPPPPGRGKMKTKQRD 253
           S    DC ++  P +DYK PE P  P P +G+ K K+ D
Sbjct: 171 SIIETDCEVDFAPPLDYKEPERPVAPAPAKGEAKAKEVD 209

>ref|NP_660506.1| NADH dehydrogenase I chain C, chain D [Buchnera aphidicola str. Sg
           (Schizaphis graminum)] gi|25008853|sp|Q8K9Y5|NUCD_BUCAP
           NADH-quinone oxidoreductase chain C/D (NADH
           dehydrogenase I, chain C/D) (NDH-1, chain C/D)
           gi|21623051|gb|AAM67717.1| NADH dehydrogenase I chain C,
           chain D [Buchnera aphidicola str. Sg (Schizaphis
           graminum)]
          Length = 597

 Score = 32.0 bits (71), Expect = 4.5
 Identities = 18/42 (42%), Positives = 24/42 (56%), Gaps = 1/42 (2%)
 Frame = -1

Query: 206 MVQDTYSPFTVFSLGSSLLLKSNEYF-SRPYPANLFFCMHKV 84
           +VQDT   F V  +  SLLLK  ++  S P P N+ F +H V
Sbjct: 36  VVQDTCIDFPVIWINKSLLLKVGKFLSSSPKPYNMLFDLHGV 77

>ref|XP_233803.1| similar to ribosomal protein L7a, cytosolic [validated] - rat
           [Rattus norvegicus]
          Length = 201

 Score = 31.2 bits (69), Expect = 7.6
 Identities = 15/32 (46%), Positives = 16/32 (49%)
 Frame = -2

Query: 286 WDRIYLFVRLGIPLLCLHFPPPWRGWKWFRIL 191
           W R  LF RL IP    H P PW G +  R L
Sbjct: 65  WQRAILFKRLKIPPAINHSPRPWTGKQRLRCL 96

>ref|NP_181689.1| putative non-LTR retroelement reverse transcriptase; protein id:
           At2g41580.1 [Arabidopsis thaliana]
           gi|7488322|pir||T00814 RNA-directed DNA polymerase
           homolog At2g41580 - Arabidopsis thaliana
           gi|2618693|gb|AAB84340.1| putative non-LTR retroelement
           reverse transcriptase [Arabidopsis thaliana]
          Length = 1094

 Score = 30.8 bits (68), Expect = 9.9
 Identities = 12/27 (44%), Positives = 17/27 (62%)
 Frame = +3

Query: 60  SVLISDWHNFMHTKKKISWIGTAKIFI 140
           SV++  W N +  KKKI WIG  K+ +
Sbjct: 549 SVMMDFWWNSVQDKKKIHWIGAQKLML 575

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 448,654,596
Number of Sequences: 1393205
Number of extensions: 9621638
Number of successful extensions: 26060
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 24714
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 26014
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 16731298976
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 GNLf017f09 BP075795 1 522
2 MPDL068g03_f AV779987 17 502




Lotus japonicus
Kazusa DNA Research Institute