Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC002778A_C01 KMC002778A_c01
(590 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAF29773.1| glutathione S-transferase [Gossypium hirsutum] 147 1e-34
pir||T09807 probable glutathione transferase (EC 2.5.1.18) - upl... 146 2e-34
ref|NP_172507.1| glutathione transferase, putative; protein id: ... 128 6e-29
gb|AAF22517.1|AF118924_1 glutathione S-transferase 1 [Papaver so... 122 3e-27
gb|AAG34795.1|AF243360_1 glutathione S-transferase GST 5 [Glycin... 121 8e-27
>gb|AAF29773.1| glutathione S-transferase [Gossypium hirsutum]
Length = 281
Score = 147 bits (371), Expect = 1e-34
Identities = 72/124 (58%), Positives = 88/124 (70%)
Frame = -3
Query: 576 KWYPAMRSIRGAKGEDERNKRIQEVREGLLLLEDAFPKSSQGHDFFGGDPIGYLDIAFGS 397
KW+P++R+I A+GED R I +V EGL+LLE+AF K SQG FG D IGYLDI FG
Sbjct: 157 KWFPSLRAIGMAEGEDARKAAIGQVEEGLMLLEEAFGKCSQGQALFGKDQIGYLDITFGC 216
Query: 396 FLGWLRVVEVSHRVKFLDPENTPGLVKWAEKFCAHIAVQDIMPETEKLHEFAKMLWAQMA 217
FLGWLRV E +K L+ NTP L+KWA +FC AV+D+MPETEKL EFAKML ++
Sbjct: 217 FLGWLRVTEKMSGIKLLNEINTPALLKWANRFCNDAAVKDVMPETEKLAEFAKMLRGRVR 276
Query: 216 AAST 205
A T
Sbjct: 277 ATPT 280
>pir||T09807 probable glutathione transferase (EC 2.5.1.18) - upland cotton
(fragment) gi|3135693|gb|AAC16555.1| glutathione
S-transferase [Gossypium hirsutum]
Length = 161
Score = 146 bits (369), Expect = 2e-34
Identities = 72/124 (58%), Positives = 88/124 (70%)
Frame = -3
Query: 576 KWYPAMRSIRGAKGEDERNKRIQEVREGLLLLEDAFPKSSQGHDFFGGDPIGYLDIAFGS 397
KW+P++R+I A+GED R I +V EGL+LLE+AF K SQG FFG D IGYLDI FG
Sbjct: 37 KWFPSLRAIGMAEGEDARKAAIGQVEEGLMLLEEAFGKCSQGQAFFGKDQIGYLDITFGC 96
Query: 396 FLGWLRVVEVSHRVKFLDPENTPGLVKWAEKFCAHIAVQDIMPETEKLHEFAKMLWAQMA 217
LGWLRV E +K L+ NTP L+KWA +FC AV+D+MPETEKL EFAKML ++
Sbjct: 97 SLGWLRVTEKMSGIKLLNEINTPALLKWANRFCNDAAVKDVMPETEKLAEFAKMLRGRVR 156
Query: 216 AAST 205
A T
Sbjct: 157 ATPT 160
>ref|NP_172507.1| glutathione transferase, putative; protein id: At1g10360.1,
supported by cDNA: gi_11096013 [Arabidopsis thaliana]
gi|11096014|gb|AAG30139.1|AF288190_1 glutathione
S-transferase [Arabidopsis thaliana]
gi|24030351|gb|AAN41340.1| putative glutathione
S-transferase TSI-1 [Arabidopsis thaliana]
Length = 227
Score = 128 bits (321), Expect = 6e-29
Identities = 66/121 (54%), Positives = 81/121 (66%)
Frame = -3
Query: 576 KWYPAMRSIRGAKGEDERNKRIQEVREGLLLLEDAFPKSSQGHDFFGGDPIGYLDIAFGS 397
+W+ ++RSI A+G++E+ I +V E LLE AF SQG FF GD IGYLDIA GS
Sbjct: 107 QWFISVRSILTAQGDEEKKAAIAQVEERTKLLEKAFNDCSQGKPFFNGDHIGYLDIALGS 166
Query: 396 FLGWLRVVEVSHRVKFLDPENTPGLVKWAEKFCAHIAVQDIMPETEKLHEFAKMLWAQMA 217
FLGW RVVE+ KFLD TP LVKWAE+FC AV+ IMPE KL EFA+ L+ +
Sbjct: 167 FLGWWRVVELDANHKFLDETKTPSLVKWAERFCDDPAVKPIMPEITKLAEFARKLFPKRQ 226
Query: 216 A 214
A
Sbjct: 227 A 227
>gb|AAF22517.1|AF118924_1 glutathione S-transferase 1 [Papaver somniferum]
gi|6652872|gb|AAF22518.1|AF118925_1 glutathione
S-transferase 2 [Papaver somniferum]
Length = 233
Score = 122 bits (307), Expect = 3e-27
Identities = 54/116 (46%), Positives = 80/116 (68%)
Frame = -3
Query: 576 KWYPAMRSIRGAKGEDERNKRIQEVREGLLLLEDAFPKSSQGHDFFGGDPIGYLDIAFGS 397
K++P++ I +K +E+ I++ +LE+A+ K+S+G DFFGG+ IGY+DIAFG
Sbjct: 110 KFFPSLMGIAKSKDAEEKKAAIEQAIAAFGILEEAYQKTSKGKDFFGGEKIGYVDIAFGC 169
Query: 396 FLGWLRVVEVSHRVKFLDPENTPGLVKWAEKFCAHIAVQDIMPETEKLHEFAKMLW 229
++GW+RV E + +K D E PGL KWAEKFCA V+ +MPET+ L EFAK ++
Sbjct: 170 YVGWIRVTEKMNGIKLFDEEKVPGLTKWAEKFCADETVKSVMPETDALMEFAKKIF 225
>gb|AAG34795.1|AF243360_1 glutathione S-transferase GST 5 [Glycine max]
Length = 234
Score = 121 bits (303), Expect = 8e-27
Identities = 59/126 (46%), Positives = 83/126 (65%), Gaps = 3/126 (2%)
Frame = -3
Query: 576 KWYPAMRSIRGAKGEDERNKRIQEVREGLLLLEDAFPKSSQGHDFFGGDPIGYLDIAFGS 397
KW+ ++RS+ A+ ++ + ++ EGL LE+ F K S+G +FGGD IG++DI FGS
Sbjct: 107 KWFTSLRSVLVAEDDEAKKPHFEQAEEGLERLEEVFNKYSEGKAYFGGDSIGFIDIGFGS 166
Query: 396 FLGWLRVVEVSHRVKFLDPENTPGLVKWAEKFCAHIAVQDIMPETEKLHEFAKML---WA 226
FL W+RV+E K LD + PGL +WAE F A AV+ I+PET+KL EFAK+L W
Sbjct: 167 FLSWMRVIEEMSGRKLLDEKKHPGLTQWAETFAADPAVKGILPETDKLVEFAKILQLKWT 226
Query: 225 QMAAAS 208
AAA+
Sbjct: 227 AAAAAA 232
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 547,261,492
Number of Sequences: 1393205
Number of extensions: 12815530
Number of successful extensions: 42683
Number of sequences better than 10.0: 219
Number of HSP's better than 10.0 without gapping: 40350
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 42611
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 22569056698
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
