KMC002767A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC002767A_C01 KMC002767A_c01
cgggcccccctcgagtttttctttttttttattaagaAACTAACTTGATTTATATTTATT
GGGCTTCGTGGAAAATGAATTAGTACATGTGTATTTATAGATACATAAGAAACCTCTTGG
AACAATGGACATACTATGAAACAGTACAACTATTGGAAAATTCTAGGTAGTGAATATAAG
ACACATGTACAGGTTAAATCCTATGAATCTCAGAACACATGAAACAAATGATGAATCAAA
TTTCATTAAATGCTGCACTAAACTCCACTTAAAATCCATTTTTTTTAACACCATCAATCA
GCAACATGTATCCCTATCACCATGATCCAGTATATACAGTAACTACCAAGTACCAATCCC
CAAACGAAGCACTAATGAATTGGGGATGCTTTACTAAACTGCTAAGAGGGAGATTTCTTC
TTTACTCTCAGAAATGGTGTACACATCAATAGCAATCCATATCCTGCAACAACATTCAGG
ATAAAGAAGACCATATGTGTGCCTGCAGTCAAATTTACAACAGTTAGCTCAAGGTAATAT
AGCTGGAAAAAGAATGGCATGTGCAAATGTTCATAAGAGTTCGCAAGTTTAGAAGTGGCA
CTCCTTTTACTTTTGAGGGATTAAACTCTTTAT


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002767A_C01 KMC002767A_c01
         (633 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_196878.2| transporter-like protein; protein id: At5g13740...    35  1.1
gb|AAM12978.1| transporter-like protein [Arabidopsis thaliana] g...    35  1.1
gb|AAM63809.1| transporter-like protein [Arabidopsis thaliana]         34  1.4
ref|NP_510830.2| Sugar transporter (53.5 kD) [Caenorhabditis ele...    34  1.4
ref|NP_568290.2| transporter-like protein; This gene structure i...    34  1.4

>ref|NP_196878.2| transporter-like protein; protein id: At5g13740.1, supported by
           cDNA: gi_20260159 [Arabidopsis thaliana]
          Length = 486

 Score = 34.7 bits (78), Expect = 1.1
 Identities = 14/30 (46%), Positives = 20/30 (66%)
 Frame = -2

Query: 503 GTHMVFFILNVVAGYGLLLMCTPFLRVKKK 414
           G+HMVFF+LNV+   G+ L   PFL   ++
Sbjct: 457 GSHMVFFVLNVIVVVGVALTFKPFLTTSRR 486

>gb|AAM12978.1| transporter-like protein [Arabidopsis thaliana]
           gi|22136244|gb|AAM91200.1| transporter-like protein
           [Arabidopsis thaliana]
          Length = 460

 Score = 34.7 bits (78), Expect = 1.1
 Identities = 14/30 (46%), Positives = 20/30 (66%)
 Frame = -2

Query: 503 GTHMVFFILNVVAGYGLLLMCTPFLRVKKK 414
           G+HMVFF+LNV+   G+ L   PFL   ++
Sbjct: 431 GSHMVFFVLNVIVVVGVALTFKPFLTTSRR 460

>gb|AAM63809.1| transporter-like protein [Arabidopsis thaliana]
          Length = 392

 Score = 34.3 bits (77), Expect = 1.4
 Identities = 16/25 (64%), Positives = 18/25 (72%)
 Frame = -2

Query: 503 GTHMVFFILNVVAGYGLLLMCTPFL 429
           GT MVFFILNVV   G++L   PFL
Sbjct: 363 GTQMVFFILNVVLALGVVLTFKPFL 387

>ref|NP_510830.2| Sugar transporter (53.5 kD) [Caenorhabditis elegans]
           gi|21629452|gb|AAM69081.1|U58737_1 Hypothetical protein
           ZK54.1 [Caenorhabditis elegans]
          Length = 479

 Score = 34.3 bits (77), Expect = 1.4
 Identities = 18/42 (42%), Positives = 29/42 (68%), Gaps = 3/42 (7%)
 Frame = -1

Query: 240 FDSSFV---SCVLRFIGFNLYMCLIFTT*NFPIVVLFHSMSI 124
           +D+ FV   +C L FIG ++++ L+ TT N  ++VLF S+SI
Sbjct: 341 YDTLFVRKMACALGFIGQSVFLFLVMTTSNSLLLVLFFSISI 382

>ref|NP_568290.2| transporter-like protein; This gene structure is inaccurate, likely
           due to discrepancies within overlapping bac sequences.
           This will be resolved asap.  In the meantime, an either
           full or partial translation is provided. [Arabidopsis
           thaliana] gi|14517414|gb|AAK62597.1| AT5g13750/MXE10_2
           [Arabidopsis thaliana] gi|17065582|gb|AAL32945.1|
           transporter-like protein [Arabidopsis thaliana]
           gi|20857393|gb|AAM26717.1| AT5g13750/MXE10_2
           [Arabidopsis thaliana]
          Length = 478

 Score = 34.3 bits (77), Expect = 1.4
 Identities = 16/25 (64%), Positives = 18/25 (72%)
 Frame = -2

Query: 503 GTHMVFFILNVVAGYGLLLMCTPFL 429
           GT MVFFILNVV   G++L   PFL
Sbjct: 449 GTQMVFFILNVVLALGVVLTFKPFL 473

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 538,055,963
Number of Sequences: 1393205
Number of extensions: 11424448
Number of successful extensions: 23104
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 22487
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23104
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 26154777244
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 GNLf009f03 BP075346 1 556
2 MRL021f06_f BP084802 38 397
3 MPDL071g01_f AV780150 48 202
4 SPDL072g05_f BP056477 48 182
5 MFBL030e02_f BP042765 234 435
6 MRL038h07_f BP085615 243 641




Lotus japonicus
Kazusa DNA Research Institute