Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC002635A_C01 KMC002635A_c01
(1091 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_201533.1| G-protein beta family; protein id: At5g67320.1,... 218 2e-55
dbj|BAC22451.1| putative G-protein beta family [Oryza sativa (ja... 207 2e-52
dbj|BAC27015.1| unnamed protein product [Mus musculus] 166 8e-40
ref|XP_135950.1| transducin (beta)-like 1 X-linked [Mus musculus... 166 8e-40
dbj|BAC27612.1| unnamed protein product [Mus musculus] 166 8e-40
>ref|NP_201533.1| G-protein beta family; protein id: At5g67320.1, supported by cDNA:
gi_16323187 [Arabidopsis thaliana]
gi|9758431|dbj|BAB09017.1|
gene_id:K8K14.4~ref|NP_005638.1~strong similarity to
unknown protein [Arabidopsis thaliana]
gi|16323188|gb|AAL15328.1| AT5g67320/K8K14_4 [Arabidopsis
thaliana] gi|23506023|gb|AAN28871.1| At5g67320/K8K14_4
[Arabidopsis thaliana]
Length = 613
Score = 218 bits (554), Expect = 2e-55
Identities = 99/118 (83%), Positives = 112/118 (94%)
Frame = -1
Query: 1091 IRWSPTGPGTSNPNKKLVLASASFDSTVKLWDVEVGKLIYSLNGHRDGVYSVAFSPNGEY 912
IRWSPTGPGT+NPNK+L LASASFDSTVKLWD E+GK++ S NGHR+ VYS+AFSPNGEY
Sbjct: 496 IRWSPTGPGTNNPNKQLTLASASFDSTVKLWDAELGKMLCSFNGHREPVYSLAFSPNGEY 555
Query: 911 LASGSPDKSIHIWSLKEGKIVKTYTGSGGIFEVCWNKEGDKIAACFANNTVCVLDFRM 738
+ASGS DKSIHIWS+KEGKIVKTYTG+GGIFEVCWNKEG+KIAACFA+N+VCVLDFRM
Sbjct: 556 IASGSLDKSIHIWSIKEGKIVKTYTGNGGIFEVCWNKEGNKIAACFADNSVCVLDFRM 613
Score = 47.4 bits (111), Expect = 4e-04
Identities = 38/133 (28%), Positives = 60/133 (44%), Gaps = 18/133 (13%)
Frame = -1
Query: 1085 WSPTGPGTSNPNKKLVLASASFDSTVKLWD--------VEVGKLIYSL---------NGH 957
WSP+ +LAS S D+T ++W V G+ I +L N
Sbjct: 273 WSPSAS---------LLASGSGDATARIWSIPEGSFKAVHTGRNINALILKHAKGKSNEK 323
Query: 956 RDGVYSVAFSPNGEYLASGSPDKSIHIWSLKEGKIVKTYTG-SGGIFEVCWNKEGDKIAA 780
V ++ ++ G LA+GS D IW+L G+++ T + G IF + WNK+GD +
Sbjct: 324 SKDVTTLDWNGEGTLLATGSCDGQARIWTLN-GELISTLSKHKGPIFSLKWNKKGDYLLT 382
Query: 779 CFANNTVCVLDFR 741
+ T V D +
Sbjct: 383 GSVDRTAVVWDVK 395
Score = 43.5 bits (101), Expect = 0.006
Identities = 35/128 (27%), Positives = 54/128 (41%), Gaps = 20/128 (15%)
Frame = -1
Query: 1043 LVLASASFDSTVKLWDVEVGKLIYSLNGHRDGVYSVAFSPNGEYLASGSPDKSIHIWSLK 864
+V+ S S + DV + L GH V + A+SP+ LASGS D + IWS+
Sbjct: 242 IVMTPTSQTSHIPNSDVRI------LEGHTSEVCACAWSPSASLLASGSGDATARIWSIP 295
Query: 863 EGKIVKTYTG------------------SGGIFEVCWNKEGDKIA--ACFANNTVCVLDF 744
EG +TG S + + WN EG +A +C + L+
Sbjct: 296 EGSFKAVHTGRNINALILKHAKGKSNEKSKDVTTLDWNGEGTLLATGSCDGQARIWTLNG 355
Query: 743 RM*SSLRR 720
+ S+L +
Sbjct: 356 ELISTLSK 363
Score = 38.5 bits (88), Expect = 0.19
Identities = 23/81 (28%), Positives = 39/81 (47%), Gaps = 1/81 (1%)
Frame = -1
Query: 1034 ASASFDSTVKLWDVEVGKLIYSLNGHRDGVYSVAFSPNGEYLASGSPDKSIHIWSLKEGK 855
A++S DS + L + + + GH+ V V + P G LAS S D + IW++K+
Sbjct: 422 ATSSTDSMIYLCKIGETRPAKTFTGHQGEVNCVKWDPTGSLLASCSDDSTAKIWNIKQST 481
Query: 854 IVKTY-TGSGGIFEVCWNKEG 795
V + I+ + W+ G
Sbjct: 482 FVHDLREHTKEIYTIRWSPTG 502
>dbj|BAC22451.1| putative G-protein beta family [Oryza sativa (japonica
cultivar-group)]
Length = 1071
Score = 207 bits (527), Expect = 2e-52
Identities = 93/118 (78%), Positives = 109/118 (91%)
Frame = -1
Query: 1091 IRWSPTGPGTSNPNKKLVLASASFDSTVKLWDVEVGKLIYSLNGHRDGVYSVAFSPNGEY 912
IRWSPTGPGT+NPN++L+LASASFDST+KLW+VE G+L+YSL GHR VYSVAFSP GEY
Sbjct: 954 IRWSPTGPGTNNPNQQLLLASASFDSTIKLWEVEQGRLLYSLAGHRQPVYSVAFSPGGEY 1013
Query: 911 LASGSPDKSIHIWSLKEGKIVKTYTGSGGIFEVCWNKEGDKIAACFANNTVCVLDFRM 738
LASGS D+ +HIWS+KEG+I+KTY GSGGIFEVCWNKEG KIAACF+NNTVC++DFRM
Sbjct: 1014 LASGSLDQCLHIWSVKEGRILKTYRGSGGIFEVCWNKEGSKIAACFSNNTVCLMDFRM 1071
Score = 48.5 bits (114), Expect = 2e-04
Identities = 26/97 (26%), Positives = 53/97 (53%), Gaps = 1/97 (1%)
Frame = -1
Query: 1040 VLASASFDSTVKLWDVEVGKLIYSLNGHRDGVYSVAFSPNGEYLASGSPDKSIHIWSLKE 861
+LA+ S+D ++W+ + G+L +L H+ ++S+ ++ G++L SGS DK+ +W K
Sbjct: 342 LLATGSYDGQARIWNSD-GELKQTLFKHKGPIFSLKWNKKGDFLLSGSVDKTAIVWDTKT 400
Query: 860 GKIVKTYT-GSGGIFEVCWNKEGDKIAACFANNTVCV 753
+ + + S +V W + + A C +N + V
Sbjct: 401 WECKQQFEFHSAPTLDVDW-RNNNSFATCSTDNMIYV 436
Score = 38.5 bits (88), Expect = 0.19
Identities = 29/111 (26%), Positives = 49/111 (44%), Gaps = 18/111 (16%)
Frame = -1
Query: 1025 SFDSTVKLWDVEVGKLIYSLNGHRDGVY-----------------SVAFSPNGEYLASGS 897
S DST ++W + G GV+ ++ ++ G LA+GS
Sbjct: 288 SGDSTARIWTIPDGPCGSITQSSPPGVHVLKHFKGRTNEKSKDVTTLDWNGEGTLLATGS 347
Query: 896 PDKSIHIWSLKEGKIVKT-YTGSGGIFEVCWNKEGDKIAACFANNTVCVLD 747
D IW+ +G++ +T + G IF + WNK+GD + + + T V D
Sbjct: 348 YDGQARIWN-SDGELKQTLFKHKGPIFSLKWNKKGDFLLSGSVDKTAIVWD 397
>dbj|BAC27015.1| unnamed protein product [Mus musculus]
Length = 527
Score = 166 bits (419), Expect = 8e-40
Identities = 74/117 (63%), Positives = 94/117 (80%)
Frame = -1
Query: 1091 IRWSPTGPGTSNPNKKLVLASASFDSTVKLWDVEVGKLIYSLNGHRDGVYSVAFSPNGEY 912
I+WSPTGP TSNPN ++LASASFDSTV+LWDVE G I++L H++ VYSVAFSP+G+Y
Sbjct: 410 IKWSPTGPATSNPNSNIMLASASFDSTVRLWDVERGVCIHTLTKHQEPVYSVAFSPDGKY 469
Query: 911 LASGSPDKSIHIWSLKEGKIVKTYTGSGGIFEVCWNKEGDKIAACFANNTVCVLDFR 741
LASGS DK +HIW+ + G +V +Y G+GGIFEVCWN GDK+ A ++ +VCVLD R
Sbjct: 470 LASGSFDKCVHIWNTQSGSLVHSYRGTGGIFEVCWNARGDKVGASASDGSVCVLDLR 526
Score = 42.7 bits (99), Expect = 0.010
Identities = 33/113 (29%), Positives = 54/113 (47%), Gaps = 15/113 (13%)
Frame = -1
Query: 1040 VLASASFDSTVKLWDVE----------VGKLIYSLNGH----RDGVYSVAFSPNGEYLAS 903
+LAS S DST ++W++ V + GH V S+ ++ +G LA+
Sbjct: 196 LLASGSGDSTARIWNLNENSNGGSTQLVLRHCIREGGHDVPSNKDVTSLDWNSDGTLLAT 255
Query: 902 GSPDKSIHIWSLKEGKIVKTY-TGSGGIFEVCWNKEGDKIAACFANNTVCVLD 747
GS D IW+ ++G + T G IF + WNK+G+ I + + T + D
Sbjct: 256 GSYDGFARIWT-EDGNLASTLGQHKGPIFALKWNKKGNYILSAGVDKTTIIWD 307
Score = 41.2 bits (95), Expect = 0.029
Identities = 23/89 (25%), Positives = 44/89 (48%), Gaps = 1/89 (1%)
Frame = -1
Query: 1040 VLASASFDSTVKLWDVEVGKLIYSLNGHRDGVYSVAFSPNGEYLASGSPDKSIHIWSLKE 861
+LA+ S+D ++W E G L +L H+ ++++ ++ G Y+ S DK+ IW
Sbjct: 252 LLATGSYDGFARIW-TEDGNLASTLGQHKGPIFALKWNKKGNYILSAGVDKTTIIWDAHT 310
Query: 860 GKIVKTYT-GSGGIFEVCWNKEGDKIAAC 777
G+ + + S +V W + A+C
Sbjct: 311 GEAKQQFPFHSAPALDVDW-QNNTTFASC 338
Score = 40.8 bits (94), Expect = 0.037
Identities = 23/81 (28%), Positives = 41/81 (50%), Gaps = 1/81 (1%)
Frame = -1
Query: 1034 ASASFDSTVKLWDVEVGKLIYSLNGHRDGVYSVAFSPNGEYLASGSPDKSIHIWSLKEGK 855
AS S D + + + + + + GH + V ++ + P+G LAS S D ++ IWS+K+
Sbjct: 336 ASCSTDMCIHVCRLGCDRPVKTFQGHTNEVNAIKWDPSGMLLASCSDDMTLKIWSMKQDA 395
Query: 854 IVKTYTG-SGGIFEVCWNKEG 795
V S I+ + W+ G
Sbjct: 396 CVHDLQAHSKEIYTIKWSPTG 416
Score = 37.7 bits (86), Expect = 0.32
Identities = 26/94 (27%), Positives = 42/94 (44%), Gaps = 1/94 (1%)
Frame = -1
Query: 1037 LASASFDSTVKLWDVEVGKLIYSLNGHRDGVYSVAFSPNGEYLASGSPDKSIHIWSLKEG 858
+ SA D T +WD G+ H V + N + AS S D IH+ L
Sbjct: 294 ILSAGVDKTTIIWDAHTGEAKQQFPFHSAPALDVDWQNNTTF-ASCSTDMCIHVCRLGCD 352
Query: 857 KIVKTYTG-SGGIFEVCWNKEGDKIAACFANNTV 759
+ VKT+ G + + + W+ G +A+C + T+
Sbjct: 353 RPVKTFQGHTNEVNAIKWDPSGMLLASCSDDMTL 386
Score = 36.6 bits (83), Expect = 0.71
Identities = 22/77 (28%), Positives = 35/77 (44%), Gaps = 15/77 (19%)
Frame = -1
Query: 968 LNGHRDGVYSVAFSPNGEYLASGSPDKSIHIWSLKEGK-------IVKTYTGSGG----- 825
L GH V+ A++P + LASGS D + IW+L E +++ GG
Sbjct: 178 LRGHESEVFICAWNPVSDLLASGSGDSTARIWNLNENSNGGSTQLVLRHCIREGGHDVPS 237
Query: 824 ---IFEVCWNKEGDKIA 783
+ + WN +G +A
Sbjct: 238 NKDVTSLDWNSDGTLLA 254
>ref|XP_135950.1| transducin (beta)-like 1 X-linked [Mus musculus]
gi|26332749|dbj|BAC30092.1| unnamed protein product [Mus
musculus] gi|27695407|gb|AAH43105.1| hypothetical protein
5330429M20 [Mus musculus]
Length = 527
Score = 166 bits (419), Expect = 8e-40
Identities = 74/117 (63%), Positives = 94/117 (80%)
Frame = -1
Query: 1091 IRWSPTGPGTSNPNKKLVLASASFDSTVKLWDVEVGKLIYSLNGHRDGVYSVAFSPNGEY 912
I+WSPTGP TSNPN ++LASASFDSTV+LWDVE G I++L H++ VYSVAFSP+G+Y
Sbjct: 410 IKWSPTGPATSNPNSNIMLASASFDSTVRLWDVERGVCIHTLTKHQEPVYSVAFSPDGKY 469
Query: 911 LASGSPDKSIHIWSLKEGKIVKTYTGSGGIFEVCWNKEGDKIAACFANNTVCVLDFR 741
LASGS DK +HIW+ + G +V +Y G+GGIFEVCWN GDK+ A ++ +VCVLD R
Sbjct: 470 LASGSFDKCVHIWNTQSGSLVHSYRGTGGIFEVCWNARGDKVGASASDGSVCVLDLR 526
Score = 42.7 bits (99), Expect = 0.010
Identities = 33/113 (29%), Positives = 54/113 (47%), Gaps = 15/113 (13%)
Frame = -1
Query: 1040 VLASASFDSTVKLWDVE----------VGKLIYSLNGH----RDGVYSVAFSPNGEYLAS 903
+LAS S DST ++W++ V + GH V S+ ++ +G LA+
Sbjct: 196 LLASGSGDSTARIWNLNENSNGGSTQLVLRHCIREGGHDVPSNKDVTSLDWNSDGTLLAT 255
Query: 902 GSPDKSIHIWSLKEGKIVKTY-TGSGGIFEVCWNKEGDKIAACFANNTVCVLD 747
GS D IW+ ++G + T G IF + WNK+G+ I + + T + D
Sbjct: 256 GSYDGFARIWT-EDGNLASTLGQHKGPIFALKWNKKGNYILSAGVDKTTIIWD 307
Score = 41.2 bits (95), Expect = 0.029
Identities = 23/89 (25%), Positives = 44/89 (48%), Gaps = 1/89 (1%)
Frame = -1
Query: 1040 VLASASFDSTVKLWDVEVGKLIYSLNGHRDGVYSVAFSPNGEYLASGSPDKSIHIWSLKE 861
+LA+ S+D ++W E G L +L H+ ++++ ++ G Y+ S DK+ IW
Sbjct: 252 LLATGSYDGFARIW-TEDGNLASTLGQHKGPIFALKWNKKGNYILSAGVDKTTIIWDAHT 310
Query: 860 GKIVKTYT-GSGGIFEVCWNKEGDKIAAC 777
G+ + + S +V W + A+C
Sbjct: 311 GEAKQQFPFHSAPALDVDW-QNNTTFASC 338
Score = 40.8 bits (94), Expect = 0.037
Identities = 23/81 (28%), Positives = 41/81 (50%), Gaps = 1/81 (1%)
Frame = -1
Query: 1034 ASASFDSTVKLWDVEVGKLIYSLNGHRDGVYSVAFSPNGEYLASGSPDKSIHIWSLKEGK 855
AS S D + + + + + + GH + V ++ + P+G LAS S D ++ IWS+K+
Sbjct: 336 ASCSTDMCIHVCRLGCDRPVKTFQGHTNEVNAIKWDPSGMLLASCSDDMTLKIWSMKQDA 395
Query: 854 IVKTYTG-SGGIFEVCWNKEG 795
V S I+ + W+ G
Sbjct: 396 CVHDLQAHSKEIYTIKWSPTG 416
Score = 37.7 bits (86), Expect = 0.32
Identities = 26/94 (27%), Positives = 42/94 (44%), Gaps = 1/94 (1%)
Frame = -1
Query: 1037 LASASFDSTVKLWDVEVGKLIYSLNGHRDGVYSVAFSPNGEYLASGSPDKSIHIWSLKEG 858
+ SA D T +WD G+ H V + N + AS S D IH+ L
Sbjct: 294 ILSAGVDKTTIIWDAHTGEAKQQFPFHSAPALDVDWQNNTTF-ASCSTDMCIHVCRLGCD 352
Query: 857 KIVKTYTG-SGGIFEVCWNKEGDKIAACFANNTV 759
+ VKT+ G + + + W+ G +A+C + T+
Sbjct: 353 RPVKTFQGHTNEVNAIKWDPSGMLLASCSDDMTL 386
Score = 36.6 bits (83), Expect = 0.71
Identities = 22/77 (28%), Positives = 35/77 (44%), Gaps = 15/77 (19%)
Frame = -1
Query: 968 LNGHRDGVYSVAFSPNGEYLASGSPDKSIHIWSLKEGK-------IVKTYTGSGG----- 825
L GH V+ A++P + LASGS D + IW+L E +++ GG
Sbjct: 178 LRGHESEVFICAWNPVSDLLASGSGDSTARIWNLNENSNGGSTQLVLRHCIREGGHDVPS 237
Query: 824 ---IFEVCWNKEGDKIA 783
+ + WN +G +A
Sbjct: 238 NKDVTSLDWNSDGTLLA 254
>dbj|BAC27612.1| unnamed protein product [Mus musculus]
Length = 412
Score = 166 bits (419), Expect = 8e-40
Identities = 74/117 (63%), Positives = 94/117 (80%)
Frame = -1
Query: 1091 IRWSPTGPGTSNPNKKLVLASASFDSTVKLWDVEVGKLIYSLNGHRDGVYSVAFSPNGEY 912
I+WSPTGP TSNPN ++LASASFDSTV+LWDVE G I++L H++ VYSVAFSP+G+Y
Sbjct: 295 IKWSPTGPATSNPNSNIMLASASFDSTVRLWDVERGVCIHTLTKHQEPVYSVAFSPDGKY 354
Query: 911 LASGSPDKSIHIWSLKEGKIVKTYTGSGGIFEVCWNKEGDKIAACFANNTVCVLDFR 741
LASGS DK +HIW+ + G +V +Y G+GGIFEVCWN GDK+ A ++ +VCVLD R
Sbjct: 355 LASGSFDKCVHIWNTQSGSLVHSYRGTGGIFEVCWNARGDKVGASASDGSVCVLDLR 411
Score = 42.7 bits (99), Expect = 0.010
Identities = 33/113 (29%), Positives = 54/113 (47%), Gaps = 15/113 (13%)
Frame = -1
Query: 1040 VLASASFDSTVKLWDVE----------VGKLIYSLNGH----RDGVYSVAFSPNGEYLAS 903
+LAS S DST ++W++ V + GH V S+ ++ +G LA+
Sbjct: 81 LLASGSGDSTARIWNLNENSNGGSTQLVLRHCIREGGHDVPSNKDVTSLDWNSDGTLLAT 140
Query: 902 GSPDKSIHIWSLKEGKIVKTY-TGSGGIFEVCWNKEGDKIAACFANNTVCVLD 747
GS D IW+ ++G + T G IF + WNK+G+ I + + T + D
Sbjct: 141 GSYDGFARIWT-EDGNLASTLGQHKGPIFALKWNKKGNYILSAGVDKTTIIWD 192
Score = 41.2 bits (95), Expect = 0.029
Identities = 23/89 (25%), Positives = 44/89 (48%), Gaps = 1/89 (1%)
Frame = -1
Query: 1040 VLASASFDSTVKLWDVEVGKLIYSLNGHRDGVYSVAFSPNGEYLASGSPDKSIHIWSLKE 861
+LA+ S+D ++W E G L +L H+ ++++ ++ G Y+ S DK+ IW
Sbjct: 137 LLATGSYDGFARIW-TEDGNLASTLGQHKGPIFALKWNKKGNYILSAGVDKTTIIWDAHT 195
Query: 860 GKIVKTYT-GSGGIFEVCWNKEGDKIAAC 777
G+ + + S +V W + A+C
Sbjct: 196 GEAKQQFPFHSAPALDVDW-QNNTTFASC 223
Score = 39.3 bits (90), Expect = 0.11
Identities = 23/81 (28%), Positives = 40/81 (48%), Gaps = 1/81 (1%)
Frame = -1
Query: 1034 ASASFDSTVKLWDVEVGKLIYSLNGHRDGVYSVAFSPNGEYLASGSPDKSIHIWSLKEGK 855
AS S D + + + + + GH + V ++ + P+G LAS S D ++ IWS+K+
Sbjct: 221 ASCSTDMCIHVCRHGCDRPVKTFQGHTNEVNAIKWDPSGMLLASCSDDMTLKIWSMKQDA 280
Query: 854 IVKTYTG-SGGIFEVCWNKEG 795
V S I+ + W+ G
Sbjct: 281 CVHDLQAHSKEIYTIKWSPTG 301
Score = 36.6 bits (83), Expect = 0.71
Identities = 22/77 (28%), Positives = 35/77 (44%), Gaps = 15/77 (19%)
Frame = -1
Query: 968 LNGHRDGVYSVAFSPNGEYLASGSPDKSIHIWSLKEGK-------IVKTYTGSGG----- 825
L GH V+ A++P + LASGS D + IW+L E +++ GG
Sbjct: 63 LRGHESEVFICAWNPVSDLLASGSGDSTARIWNLNENSNGGSTQLVLRHCIREGGHDVPS 122
Query: 824 ---IFEVCWNKEGDKIA 783
+ + WN +G +A
Sbjct: 123 NKDVTSLDWNSDGTLLA 139
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 881,910,263
Number of Sequences: 1393205
Number of extensions: 19331603
Number of successful extensions: 56967
Number of sequences better than 10.0: 2659
Number of HSP's better than 10.0 without gapping: 44646
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 54706
length of database: 448,689,247
effective HSP length: 125
effective length of database: 274,538,622
effective search space used: 65340192036
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
