Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC002591A_C01 KMC002591A_c01
(1224 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAK25949.1|AF360239_1 putative ER lumen protein retaining rec... 389 e-130
ref|NP_565501.1| putative ER lumen protein retaining receptor; p... 388 e-130
gb|AAM61627.1| putative ER lumen protein retaining receptor [Ara... 390 e-130
ref|NP_568047.1| expressed protein; protein id: At4g38790.1, sup... 384 e-129
ref|NP_173426.1| ER lumen protein-retaining receptor; protein id... 374 e-123
>gb|AAK25949.1|AF360239_1 putative ER lumen protein retaining receptor [Arabidopsis thaliana]
Length = 269
Score = 389 bits (1000), Expect(2) = e-130
Identities = 190/224 (84%), Positives = 204/224 (90%)
Frame = -1
Query: 1158 RMSIHTVATWVRRQPPKVKAFLAVVSGMAALVLLRFIVHDHDNLFVAAEAVHSLGISVLI 979
R IH V+TWVRRQPPKVKAFLAVVSGMAALVLL+ IVHDHDNLFVAAEAVHS+GISVLI
Sbjct: 2 RTPIHAVSTWVRRQPPKVKAFLAVVSGMAALVLLKLIVHDHDNLFVAAEAVHSIGISVLI 61
Query: 978 YKLMKEKTCAGLSLKSQELTAMFLAVRLYCSFVMEYDIHTLLDFATFATTVWVIYMIRFK 799
YKLMKEKTCAGLSLKSQELTA+FLAVRLYCSFVMEYDIHT+LD AT TT+WVIYMIRF
Sbjct: 62 YKLMKEKTCAGLSLKSQELTAIFLAVRLYCSFVMEYDIHTILDLATLGTTLWVIYMIRFN 121
Query: 798 LKASYMEEKDNFAIYYVVIPCAVLALLIHPSTSHHILNRIFWAFCVYLEAVSVLPQLRVM 619
LK +YMEEKDNFA+YYV+ PC VLA+ IHPSTSH+ILNRI W FCVYLEAVSVLPQLRVM
Sbjct: 122 LKTTYMEEKDNFALYYVLAPCVVLAVWIHPSTSHNILNRISWGFCVYLEAVSVLPQLRVM 181
Query: 618 QNTKIVEPFTAHYVFALGVARFLSCAHWVLQVLDTRGTFVGCLG 487
QNTKIVEPFTAHYVFALGVARF SCAHWVLQ++DT G + LG
Sbjct: 182 QNTKIVEPFTAHYVFALGVARFFSCAHWVLQMMDTHGRLLVVLG 225
Score = 100 bits (249), Expect(2) = e-130
Identities = 48/50 (96%), Positives = 49/50 (98%)
Frame = -2
Query: 503 LLVALGYGLWPSMVLISEIVQTFILADFCYYYVKSVFGGQLVLRLPSGVV 354
LLV LGYGLWPSMV+ISEIVQTFILADFCYYYVKSVFGGQLVLRLPSGVV
Sbjct: 220 LLVVLGYGLWPSMVIISEIVQTFILADFCYYYVKSVFGGQLVLRLPSGVV 269
>ref|NP_565501.1| putative ER lumen protein retaining receptor; protein id:
At2g21190.1, supported by cDNA: 126278., supported by
cDNA: gi_13430653 [Arabidopsis thaliana]
gi|20197720|gb|AAD29797.2| putative ER lumen protein
retaining receptor [Arabidopsis thaliana]
Length = 269
Score = 388 bits (997), Expect(2) = e-130
Identities = 189/221 (85%), Positives = 203/221 (91%)
Frame = -1
Query: 1149 IHTVATWVRRQPPKVKAFLAVVSGMAALVLLRFIVHDHDNLFVAAEAVHSLGISVLIYKL 970
IH V+TWVRRQPPKVKAFLAVVSGMAALVLL+ IVHDHDNLFVAAEAVHS+GISVLIYKL
Sbjct: 5 IHAVSTWVRRQPPKVKAFLAVVSGMAALVLLKLIVHDHDNLFVAAEAVHSIGISVLIYKL 64
Query: 969 MKEKTCAGLSLKSQELTAMFLAVRLYCSFVMEYDIHTLLDFATFATTVWVIYMIRFKLKA 790
MKEKTCAGLSLKSQELTA+FLAVRLYCSFVMEYDIHT+LD AT TT+WVIYMIRF LK
Sbjct: 65 MKEKTCAGLSLKSQELTAIFLAVRLYCSFVMEYDIHTILDLATLGTTLWVIYMIRFNLKT 124
Query: 789 SYMEEKDNFAIYYVVIPCAVLALLIHPSTSHHILNRIFWAFCVYLEAVSVLPQLRVMQNT 610
+YMEEKDNFA+YYV+ PC VLA+ IHPSTSH+ILNRI W FCVYLEAVSVLPQLRVMQNT
Sbjct: 125 TYMEEKDNFALYYVLAPCVVLAVWIHPSTSHNILNRISWGFCVYLEAVSVLPQLRVMQNT 184
Query: 609 KIVEPFTAHYVFALGVARFLSCAHWVLQVLDTRGTFVGCLG 487
KIVEPFTAHYVFALGVARF SCAHWVLQ++DT G + LG
Sbjct: 185 KIVEPFTAHYVFALGVARFFSCAHWVLQMMDTHGRLLVVLG 225
Score = 100 bits (249), Expect(2) = e-130
Identities = 48/50 (96%), Positives = 49/50 (98%)
Frame = -2
Query: 503 LLVALGYGLWPSMVLISEIVQTFILADFCYYYVKSVFGGQLVLRLPSGVV 354
LLV LGYGLWPSMV+ISEIVQTFILADFCYYYVKSVFGGQLVLRLPSGVV
Sbjct: 220 LLVVLGYGLWPSMVIISEIVQTFILADFCYYYVKSVFGGQLVLRLPSGVV 269
>gb|AAM61627.1| putative ER lumen protein retaining receptor [Arabidopsis thaliana]
Length = 269
Score = 390 bits (1001), Expect(2) = e-130
Identities = 190/221 (85%), Positives = 204/221 (91%)
Frame = -1
Query: 1149 IHTVATWVRRQPPKVKAFLAVVSGMAALVLLRFIVHDHDNLFVAAEAVHSLGISVLIYKL 970
IH V+TWVRRQPPKVKAFLAVVSGMAALVLL+ IVHDHDNLFVAAEAVHS+GISVLIYKL
Sbjct: 5 IHAVSTWVRRQPPKVKAFLAVVSGMAALVLLKLIVHDHDNLFVAAEAVHSIGISVLIYKL 64
Query: 969 MKEKTCAGLSLKSQELTAMFLAVRLYCSFVMEYDIHTLLDFATFATTVWVIYMIRFKLKA 790
MKEKTCAGLSLKSQELTA+FLAVRLYCSFVMEYDIHT+LD AT TT+WVIYMIRF LKA
Sbjct: 65 MKEKTCAGLSLKSQELTAIFLAVRLYCSFVMEYDIHTILDLATLGTTLWVIYMIRFNLKA 124
Query: 789 SYMEEKDNFAIYYVVIPCAVLALLIHPSTSHHILNRIFWAFCVYLEAVSVLPQLRVMQNT 610
+YMEEKDNFA+YYV+ PC VLA+ IHPSTSH+ILNRI W FCVYLEAVSVLPQLRVMQNT
Sbjct: 125 TYMEEKDNFALYYVLAPCVVLAVWIHPSTSHNILNRISWGFCVYLEAVSVLPQLRVMQNT 184
Query: 609 KIVEPFTAHYVFALGVARFLSCAHWVLQVLDTRGTFVGCLG 487
KIVEPFTAHYVFALGVARF SCAHWVLQ++DT G + LG
Sbjct: 185 KIVEPFTAHYVFALGVARFFSCAHWVLQMMDTHGRLLVVLG 225
Score = 98.6 bits (244), Expect(2) = e-130
Identities = 47/50 (94%), Positives = 48/50 (96%)
Frame = -2
Query: 503 LLVALGYGLWPSMVLISEIVQTFILADFCYYYVKSVFGGQLVLRLPSGVV 354
LLV LGYGLWPSMV+ISEIVQ FILADFCYYYVKSVFGGQLVLRLPSGVV
Sbjct: 220 LLVVLGYGLWPSMVIISEIVQAFILADFCYYYVKSVFGGQLVLRLPSGVV 269
>ref|NP_568047.1| expressed protein; protein id: At4g38790.1, supported by cDNA: 21759.
[Arabidopsis thaliana] gi|21554272|gb|AAM63347.1|
putative ER lumen protein retaining receptor [Arabidopsis
thaliana]
Length = 273
Score = 384 bits (987), Expect(2) = e-129
Identities = 187/221 (84%), Positives = 203/221 (91%)
Frame = -1
Query: 1149 IHTVATWVRRQPPKVKAFLAVVSGMAALVLLRFIVHDHDNLFVAAEAVHSLGISVLIYKL 970
IH V +WVRRQPPKVKAFLAVV+GMAALVLLRFIVHDHDNLFVAAEAVHS+GI VLIYKL
Sbjct: 9 IHVVWSWVRRQPPKVKAFLAVVTGMAALVLLRFIVHDHDNLFVAAEAVHSIGICVLIYKL 68
Query: 969 MKEKTCAGLSLKSQELTAMFLAVRLYCSFVMEYDIHTLLDFATFATTVWVIYMIRFKLKA 790
MKEKTCAGLSLKSQELTA+FLAVRLYCS VMEYDIHT+LD AT TT+WVI+MIRFKL+A
Sbjct: 69 MKEKTCAGLSLKSQELTAIFLAVRLYCSIVMEYDIHTILDLATLGTTLWVIFMIRFKLRA 128
Query: 789 SYMEEKDNFAIYYVVIPCAVLALLIHPSTSHHILNRIFWAFCVYLEAVSVLPQLRVMQNT 610
SYME+KDNF +YYV+ PC LA+ IHPSTSH+I+NRI WA CVYLEAVSVLPQLRVMQNT
Sbjct: 129 SYMEDKDNFPLYYVLAPCVALAVFIHPSTSHNIINRISWALCVYLEAVSVLPQLRVMQNT 188
Query: 609 KIVEPFTAHYVFALGVARFLSCAHWVLQVLDTRGTFVGCLG 487
KIVEPFTAHYVFALGVARFLSCAHWVLQV+DTRG + LG
Sbjct: 189 KIVEPFTAHYVFALGVARFLSCAHWVLQVVDTRGRLLVALG 229
Score = 102 bits (255), Expect(2) = e-129
Identities = 50/50 (100%), Positives = 50/50 (100%)
Frame = -2
Query: 503 LLVALGYGLWPSMVLISEIVQTFILADFCYYYVKSVFGGQLVLRLPSGVV 354
LLVALGYGLWPSMVLISEIVQTFILADFCYYYVKSVFGGQLVLRLPSGVV
Sbjct: 224 LLVALGYGLWPSMVLISEIVQTFILADFCYYYVKSVFGGQLVLRLPSGVV 273
>ref|NP_173426.1| ER lumen protein-retaining receptor; protein id: At1g19970.1
[Arabidopsis thaliana]
Length = 272
Score = 374 bits (959), Expect(2) = e-123
Identities = 176/228 (77%), Positives = 203/228 (88%)
Frame = -1
Query: 1170 MRPQRMSIHTVATWVRRQPPKVKAFLAVVSGMAALVLLRFIVHDHDNLFVAAEAVHSLGI 991
M+ ++ I +V W+RRQPPKVKAFL VV+ M LVLLR IVH+HDNLFVA+EAVH++GI
Sbjct: 1 MKAEKNPIQSVMAWIRRQPPKVKAFLGVVTAMTVLVLLRVIVHEHDNLFVASEAVHAVGI 60
Query: 990 SVLIYKLMKEKTCAGLSLKSQELTAMFLAVRLYCSFVMEYDIHTLLDFATFATTVWVIYM 811
+VLIYKL KEKTCAG+SLK+QELTA+FLAVRLYCSFVME+D+HT LD AT TT+WV+YM
Sbjct: 61 AVLIYKLTKEKTCAGISLKTQELTALFLAVRLYCSFVMEFDLHTFLDSATLVTTLWVVYM 120
Query: 810 IRFKLKASYMEEKDNFAIYYVVIPCAVLALLIHPSTSHHILNRIFWAFCVYLEAVSVLPQ 631
IRFKL+ +YME+KDNFAIY+VVIPCAVL+ IHPST HHI+NRI WAFCVYLEAVSVLPQ
Sbjct: 121 IRFKLRPTYMEDKDNFAIYFVVIPCAVLSFFIHPSTRHHIINRISWAFCVYLEAVSVLPQ 180
Query: 630 LRVMQNTKIVEPFTAHYVFALGVARFLSCAHWVLQVLDTRGTFVGCLG 487
LRVMQNTKIVEPFTAHYVFALG+ARFLSCAHW+LQVLDTRG + LG
Sbjct: 181 LRVMQNTKIVEPFTAHYVFALGIARFLSCAHWILQVLDTRGRLLTALG 228
Score = 90.5 bits (223), Expect(2) = e-123
Identities = 44/50 (88%), Positives = 46/50 (92%)
Frame = -2
Query: 503 LLVALGYGLWPSMVLISEIVQTFILADFCYYYVKSVFGGQLVLRLPSGVV 354
LL ALG GLWP MVLISEIVQTFILADFCYYYV+S+ GGQLVLRLPSGVV
Sbjct: 223 LLTALGSGLWPVMVLISEIVQTFILADFCYYYVQSLMGGQLVLRLPSGVV 272
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,086,462,106
Number of Sequences: 1393205
Number of extensions: 25260385
Number of successful extensions: 73522
Number of sequences better than 10.0: 88
Number of HSP's better than 10.0 without gapping: 65634
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 72712
length of database: 448,689,247
effective HSP length: 126
effective length of database: 273,145,417
effective search space used: 76753862177
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
