Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC002538A_C01 KMC002538A_c01
(673 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||T50555 delta-8 sphingolipid desaturase [imported] - rape gi... 224 6e-58
ref|NP_182144.1| putative fatty acid desaturase/cytochrome b5 fu... 224 8e-58
pir||S68358 Delta8 sphingolipid desaturase (EC 1.14.99.-) [simil... 223 1e-57
gb|AAG43277.1|AF133728_1 delta 8-sphingolipid desaturase [Borago... 220 2e-56
ref|NP_191717.1| delta-8 sphingolipid desaturase; protein id: At... 214 9e-55
>pir||T50555 delta-8 sphingolipid desaturase [imported] - rape
gi|3819708|emb|CAA11857.1| delta-8 sphingolipid
desaturase [Brassica napus]
Length = 449
Score = 224 bits (572), Expect = 6e-58
Identities = 100/133 (75%), Positives = 117/133 (87%)
Frame = -1
Query: 667 SFVVCSVQHLQFCLNHFAANVYVGPPKGNDWFEKQTSGTLDISCSSWMDWFFGGLQFQLE 488
S V ++QH+QFCLNHFAA+VY GPP GNDWFEKQT+GTLDISC S+MDWFFGGLQFQLE
Sbjct: 317 SMAVTAIQHVQFCLNHFAADVYTGPPNGNDWFEKQTAGTLDISCRSYMDWFFGGLQFQLE 376
Query: 487 HHLFPRLPRAQLRKISPLVIDLCKKHNLPYRSLSFVEANLWTLKTLRTAALQARNLIDPS 308
HHLFPRLPR LR +SP+V +LCKKHNLPYRSLS+ EAN+WTL+TLR AA+QAR++ +P
Sbjct: 377 HHLFPRLPRCHLRGVSPVVQELCKKHNLPYRSLSWWEANVWTLRTLRKAAVQARDVTNPV 436
Query: 307 SQNLLWEAFNTHG 269
+NLLWEA NTHG
Sbjct: 437 LENLLWEALNTHG 449
>ref|NP_182144.1| putative fatty acid desaturase/cytochrome b5 fusion protein;
protein id: At2g46210.1 [Arabidopsis thaliana]
gi|25408976|pir||A84900 hypothetical protein At2g46210
[imported] - Arabidopsis thaliana
gi|3702328|gb|AAC62885.1| putative fatty acid
desaturase/cytochrome b5 fusion protein [Arabidopsis
thaliana]
Length = 449
Score = 224 bits (571), Expect = 8e-58
Identities = 99/133 (74%), Positives = 117/133 (87%)
Frame = -1
Query: 667 SFVVCSVQHLQFCLNHFAANVYVGPPKGNDWFEKQTSGTLDISCSSWMDWFFGGLQFQLE 488
SF V ++QH+QFCLNHFAA+VY GPP GNDWFEKQT+GTLDISC S+MDWFFGGLQFQLE
Sbjct: 317 SFAVTAIQHVQFCLNHFAADVYTGPPNGNDWFEKQTAGTLDISCRSFMDWFFGGLQFQLE 376
Query: 487 HHLFPRLPRAQLRKISPLVIDLCKKHNLPYRSLSFVEANLWTLKTLRTAALQARNLIDPS 308
HHLFPRLPR LR +SP+V +LCKKHNLPYRSLS+ EAN+WT++TL+ AA+QAR+ +P
Sbjct: 377 HHLFPRLPRCHLRTVSPVVKELCKKHNLPYRSLSWWEANVWTIRTLKNAAIQARDATNPV 436
Query: 307 SQNLLWEAFNTHG 269
+NLLWEA NTHG
Sbjct: 437 LKNLLWEAVNTHG 449
>pir||S68358 Delta8 sphingolipid desaturase (EC 1.14.99.-) [similarity] - common
sunflower gi|1040729|emb|CAA60621.1| delta-8
sphingolipid desaturase [Helianthus annuus]
Length = 458
Score = 223 bits (569), Expect = 1e-57
Identities = 101/135 (74%), Positives = 117/135 (85%)
Frame = -1
Query: 673 LASFVVCSVQHLQFCLNHFAANVYVGPPKGNDWFEKQTSGTLDISCSSWMDWFFGGLQFQ 494
L SF V +QH+QF LNHF+ +VYVGPPKG++WFEKQT GT+DI+CSSWMDWFFGGLQFQ
Sbjct: 324 LVSFCVTGIQHIQFTLNHFSGDVYVGPPKGDNWFEKQTRGTIDIACSSWMDWFFGGLQFQ 383
Query: 493 LEHHLFPRLPRAQLRKISPLVIDLCKKHNLPYRSLSFVEANLWTLKTLRTAALQARNLID 314
LEHHLFPRLPR LR ISP+ +LCKK+NLPY SLSF +AN+ TLKTLRTAALQAR+L +
Sbjct: 384 LEHHLFPRLPRCHLRSISPICRELCKKYNLPYVSLSFYDANVTTLKTLRTAALQARDLTN 443
Query: 313 PSSQNLLWEAFNTHG 269
P+ QNL WEAFNTHG
Sbjct: 444 PAPQNLAWEAFNTHG 458
>gb|AAG43277.1|AF133728_1 delta 8-sphingolipid desaturase [Borago officinalis]
Length = 446
Score = 220 bits (560), Expect = 2e-56
Identities = 103/135 (76%), Positives = 116/135 (85%)
Frame = -1
Query: 673 LASFVVCSVQHLQFCLNHFAANVYVGPPKGNDWFEKQTSGTLDISCSSWMDWFFGGLQFQ 494
L F V ++QH+QF LNHFAA+VYVGPP G +WFEKQ +GT+DISCSSWMDWFFGGLQFQ
Sbjct: 313 LTCFSVTALQHIQFTLNHFAADVYVGPPTGTNWFEKQAAGTIDISCSSWMDWFFGGLQFQ 372
Query: 493 LEHHLFPRLPRAQLRKISPLVIDLCKKHNLPYRSLSFVEANLWTLKTLRTAALQARNLID 314
LEHHLFPR+PR QLR ISP+V D CKKHNLPYRSLSF +AN+ T+KTLRTAALQAR+L
Sbjct: 373 LEHHLFPRMPRCQLRNISPIVQDYCKKHNLPYRSLSFFDANVATIKTLRTAALQARDL-T 431
Query: 313 PSSQNLLWEAFNTHG 269
QNLLWEAFNTHG
Sbjct: 432 VVPQNLLWEAFNTHG 446
>ref|NP_191717.1| delta-8 sphingolipid desaturase; protein id: At3g61580.1, supported
by cDNA: 34427., supported by cDNA: gi_16226516,
supported by cDNA: gi_4100568 [Arabidopsis thaliana]
gi|11357245|pir||T47950 delta-8 sphingolipid desaturase
(EC 1.14.99.-) [validated] - Arabidopsis thaliana
gi|3819710|emb|CAA11858.1| delta-8 sphingolipid
desaturase [Arabidopsis thaliana]
gi|6850849|emb|CAB71088.1| delta-8 sphingolipid
desaturase [Arabidopsis thaliana]
gi|16226517|gb|AAL16189.1|AF428420_1 AT3g61580/F2A19_180
[Arabidopsis thaliana] gi|23306384|gb|AAN17419.1|
delta-8 sphingolipid desaturase [Arabidopsis thaliana]
gi|28059272|gb|AAO30042.1| delta-8 sphingolipid
desaturase [Arabidopsis thaliana]
Length = 449
Score = 214 bits (545), Expect = 9e-55
Identities = 95/133 (71%), Positives = 116/133 (86%)
Frame = -1
Query: 667 SFVVCSVQHLQFCLNHFAANVYVGPPKGNDWFEKQTSGTLDISCSSWMDWFFGGLQFQLE 488
SF V ++QH+QF LNHFAA+VYVGPP G+DWFEKQ +GT+DISC S+MDWFFGGLQFQLE
Sbjct: 317 SFTVTALQHIQFTLNHFAADVYVGPPTGSDWFEKQAAGTIDISCRSYMDWFFGGLQFQLE 376
Query: 487 HHLFPRLPRAQLRKISPLVIDLCKKHNLPYRSLSFVEANLWTLKTLRTAALQARNLIDPS 308
HHLFPRLPR LRK+SP+V +LCKKHNLPYRS+S+ EAN+ T+ TL+TAA QAR++ +P
Sbjct: 377 HHLFPRLPRCHLRKVSPVVQELCKKHNLPYRSMSWFEANVLTINTLKTAAYQARDVANPV 436
Query: 307 SQNLLWEAFNTHG 269
+NL+WEA NTHG
Sbjct: 437 VKNLVWEALNTHG 449
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 574,294,862
Number of Sequences: 1393205
Number of extensions: 12308741
Number of successful extensions: 36312
Number of sequences better than 10.0: 184
Number of HSP's better than 10.0 without gapping: 34870
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 36291
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 29421376608
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
