KMC002306A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC002306A_C01 KMC002306A_c01
ggctaaaaggggactgaaacatttttattatacagccactattATGTACCTTTGAAGGCA
TGTCCTGCGGCTAATACCATAAAATAGTGTAAATGACTTACATTAATTAGCATGGTATGG
AAAACAGAGTCAGAGCTGAACCACAGCAAATTTGAGCCTAAACAATGGTGCTAGTAAGAT
TCTAGGATCCATCAAATTGGCTAGTATAAATAATATACAGCAAAATTTGCTGCAAGTTCT
AAGTTAGTTCAGCATCCAGCAGCTTCTAGTGCTGCACTCCAGTGAGAATGAGATATTCAC
AATATCTTGCCAGGGCAAAAAATAACCTTGGATTCTTGTTACTTAGGAGGGGGGACAAAA
CAAATCAGAGAAAGTAGAAGGCCCCAAAAGAAAAAAACCAGCAGAGAATTTATAAGGAGG
TTGAATGCAGCATCCTGTACCATAACTCAAAGGTAGATCATGTCCCATAGTAGTCACCCA
AGTTGTCAACAAGGTAGTCATATGGGGAAGCTAACCCATACTCATATGTTCCGTTCAGTG
GAGTCACTCTGTGTGAATAGACACCACCCATGGCAACTCTGGCATTGATTTTGGCTCTGC
CTTTAGCAGGCCTTTTGTTATCT


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC002306A_C01 KMC002306A_c01
         (623 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAF24553.1|AC007508_16 F1K23.24 [Arabidopsis thaliana]              43  0.003
ref|NP_174174.1| unknown protein; protein id: At1g28520.1 [Arabi...    43  0.003
ref|NP_571891.1| atonal homolog 2a [Danio rerio] gi|4566748|gb|A...    33  4.0
ref|NP_635821.1| dihydrolipoamide acyltransferase [Xanthomonas c...    32  8.8
ref|NP_640798.1| dihydrolipoamide acyltransferase [Xanthomonas a...    32  8.8

>gb|AAF24553.1|AC007508_16 F1K23.24 [Arabidopsis thaliana]
          Length = 190

 Score = 43.1 bits (100), Expect = 0.003
 Identities = 21/52 (40%), Positives = 31/52 (59%)
 Frame = -2

Query: 622 DNKRPAKGRAKINARVAMGGVYSHRVTPLNGTYEYGLASPYDYLVDNLGDYY 467
           +NKR  KGR K++ +VA G V +     +    +YG+   ++YLV NL DYY
Sbjct: 141 NNKRCIKGRPKVSTKVATGNVQN----TVEQANDYGVGEEFNYLVGNLSDYY 188

>ref|NP_174174.1| unknown protein; protein id: At1g28520.1 [Arabidopsis thaliana]
           gi|25370819|pir||B86411 protein F3M18.4 [imported] -
           Arabidopsis thaliana
           gi|6560771|gb|AAF16771.1|AC010155_24 F3M18.4
           [Arabidopsis thaliana]
          Length = 486

 Score = 43.1 bits (100), Expect = 0.003
 Identities = 21/52 (40%), Positives = 31/52 (59%)
 Frame = -2

Query: 622 DNKRPAKGRAKINARVAMGGVYSHRVTPLNGTYEYGLASPYDYLVDNLGDYY 467
           +NKR  KGR K++ +VA G V +     +    +YG+   ++YLV NL DYY
Sbjct: 437 NNKRCIKGRPKVSTKVATGNVQN----TVEQANDYGVGEEFNYLVGNLSDYY 484

>ref|NP_571891.1| atonal homolog 2a [Danio rerio] gi|4566748|gb|AAD23441.1|AF115772_1
           basic helix-loop-helix transcription factor Ndr1a [Danio
           rerio]
          Length = 325

 Score = 32.7 bits (73), Expect = 4.0
 Identities = 20/61 (32%), Positives = 29/61 (46%)
 Frame = -3

Query: 477 VTTMGHDLPLSYGTGCCIQPPYKFSAGFFLLGPSTFSDLFCPPS*VTRIQGYFLPWQDIV 298
           + ++ H+ P+ YG  C     Y  SA F    P+  SDL  P      ++G F P QD +
Sbjct: 263 IFSLKHEEPVEYGKSCHYGTRYWRSAAFHRPEPAGSSDLHFPYD--IHLRGQFYPVQDEL 320

Query: 297 N 295
           N
Sbjct: 321 N 321

>ref|NP_635821.1| dihydrolipoamide acyltransferase [Xanthomonas campestris pv.
           campestris str. ATCC 33913] gi|21111411|gb|AAM39745.1|
           dihydrolipoamide acyltransferase [Xanthomonas campestris
           pv. campestris str. ATCC 33913]
          Length = 502

 Score = 31.6 bits (70), Expect = 8.8
 Identities = 14/34 (41%), Positives = 22/34 (64%)
 Frame = -2

Query: 607 AKGRAKINARVAMGGVYSHRVTPLNGTYEYGLAS 506
           A GRA+      MGGV +H+V PL+ T+++  A+
Sbjct: 450 AAGRARYQLTPVMGGVETHKVMPLSLTFDHRAAT 483

>ref|NP_640798.1| dihydrolipoamide acyltransferase [Xanthomonas axonopodis pv. citri
           str. 306] gi|21106528|gb|AAM35334.1| dihydrolipoamide
           acyltransferase [Xanthomonas axonopodis pv. citri str.
           306]
          Length = 505

 Score = 31.6 bits (70), Expect = 8.8
 Identities = 14/34 (41%), Positives = 22/34 (64%)
 Frame = -2

Query: 607 AKGRAKINARVAMGGVYSHRVTPLNGTYEYGLAS 506
           A GRA+      MGGV +H+V PL+ T+++  A+
Sbjct: 453 AAGRARYQLTPVMGGVETHKVMPLSLTFDHRAAT 486

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 530,434,937
Number of Sequences: 1393205
Number of extensions: 10905960
Number of successful extensions: 25371
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 24841
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25364
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 25301904073
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 GENf053c07 BP060602 1 473
2 SPDL078d07_f BP056843 44 537
3 MWM244g12_f AV768466 86 623
4 MWM110h01_f AV766490 86 593




Lotus japonicus
Kazusa DNA Research Institute