Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC002032A_C02 KMC002032A_c02
(726 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAC58013.1| S-adenosyl-L-methionine: 2,7,4'-trihydroxyisofla... 234 7e-61
dbj|BAC58011.1| S-adenosyl-L-methionine: 2,7,4'-trihydroxyisofla... 216 3e-55
pir||T06786 6a-hydroxymaackiain methyltransferase (EC 2.1.1.-) -... 202 4e-51
gb|AAM23005.1|AF502434_1 orcinol O-methyltransferase [Rosa hybri... 141 1e-32
gb|AAB71213.1| methyltransferase [Prunus armeniaca] 139 3e-32
>dbj|BAC58013.1| S-adenosyl-L-methionine: 2,7,4'-trihydroxyisoflavanone
4'-O-methyltransferase [Lotus japonicus]
Length = 365
Score = 234 bits (598), Expect = 7e-61
Identities = 119/140 (85%), Positives = 128/140 (91%)
Frame = -3
Query: 724 CTVLDQPQLVANLTGDENLNFVGGDMFKSVPPADAVLLKWVLHDWNDELSLKILKNCKEA 545
CTV DQPQ+VANLTGDENLNFVGGDMFKSVPPADAVLLKWVLHDWNDELSLKILKNCKEA
Sbjct: 227 CTVFDQPQVVANLTGDENLNFVGGDMFKSVPPADAVLLKWVLHDWNDELSLKILKNCKEA 286
Query: 544 ISGRGKEGKVIIIDISIDETSDDRELTELKLDYDLVMLTMFNGQRKREERMGEAHI*CRL 365
ISGRGKEGKVIIIDISIDETSDDRELTELKLDYDLVMLTMFNG ++RE++ E I
Sbjct: 287 ISGRGKEGKVIIIDISIDETSDDRELTELKLDYDLVMLTMFNG-KEREKKEWEKLIYDAG 345
Query: 364 PAATRVTPICGFKSLIEVFP 305
++ ++TPICGFKSLIEVFP
Sbjct: 346 FSSYKITPICGFKSLIEVFP 365
>dbj|BAC58011.1| S-adenosyl-L-methionine: 2,7,4'-trihydroxyisoflavanone
4'-O-methyltransferase [Glycyrrhiza echinata]
Length = 367
Score = 216 bits (549), Expect = 3e-55
Identities = 109/140 (77%), Positives = 123/140 (87%)
Frame = -3
Query: 724 CTVLDQPQLVANLTGDENLNFVGGDMFKSVPPADAVLLKWVLHDWNDELSLKILKNCKEA 545
CTV DQPQ+V NL+G+ENL FVGGDMFKS+PPADAVLLKWVLHDWNDELSLKILKN KEA
Sbjct: 229 CTVFDQPQVVGNLSGNENLKFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNSKEA 288
Query: 544 ISGRGKEGKVIIIDISIDETSDDRELTELKLDYDLVMLTMFNGQRKREERMGEAHI*CRL 365
ISG+GKEGKVIIIDISIDE S DRELTEL+LDYDLVMLTMFNG ++RE++ E I
Sbjct: 289 ISGKGKEGKVIIIDISIDEASGDRELTELQLDYDLVMLTMFNG-KEREKKEWEKLISDAG 347
Query: 364 PAATRVTPICGFKSLIEVFP 305
++ ++TPICGFKSLIEVFP
Sbjct: 348 FSSYKITPICGFKSLIEVFP 367
>pir||T06786 6a-hydroxymaackiain methyltransferase (EC 2.1.1.-) - garden pea
gi|1568637|gb|AAC49856.1| 6a-hydroxymaackiain
methyltransferase [Pisum sativum]
Length = 360
Score = 202 bits (514), Expect = 4e-51
Identities = 104/140 (74%), Positives = 119/140 (84%)
Frame = -3
Query: 724 CTVLDQPQLVANLTGDENLNFVGGDMFKSVPPADAVLLKWVLHDWNDELSLKILKNCKEA 545
CTV DQPQ+V NLTG+ENLNFVGGDMFKSVP ADAVLLKWVLHDWNDELSLKILKN KEA
Sbjct: 222 CTVFDQPQVVGNLTGNENLNFVGGDMFKSVPSADAVLLKWVLHDWNDELSLKILKNSKEA 281
Query: 544 ISGRGKEGKVIIIDISIDETSDDRELTELKLDYDLVMLTMFNGQRKREERMGEAHI*CRL 365
IS +GK+GKVIIIDISIDE SDDR LTEL+L+YD+VMLTMF G ++R ++ E I
Sbjct: 282 ISHKGKDGKVIIIDISIDENSDDRGLTELQLEYDVVMLTMFLG-KERTKKEWEKLIYDAG 340
Query: 364 PAATRVTPICGFKSLIEVFP 305
+ ++TPICGFKSLIEV+P
Sbjct: 341 FSRYKITPICGFKSLIEVYP 360
>gb|AAM23005.1|AF502434_1 orcinol O-methyltransferase [Rosa hybrid cultivar]
gi|27527924|emb|CAD29459.1| orcinol O-methyltransferase
[Rosa chinensis]
Length = 366
Score = 141 bits (355), Expect = 1e-32
Identities = 67/140 (47%), Positives = 96/140 (67%)
Frame = -3
Query: 724 CTVLDQPQLVANLTGDENLNFVGGDMFKSVPPADAVLLKWVLHDWNDELSLKILKNCKEA 545
CTVLD P +VA+L G +NL + GGDMF++VPPAD VLLKW+LHDWNDE +KILK + A
Sbjct: 228 CTVLDLPHVVADLQGSKNLKYTGGDMFEAVPPADTVLLKWILHDWNDEECIKILKRSRVA 287
Query: 544 ISGRGKEGKVIIIDISIDETSDDRELTELKLDYDLVMLTMFNGQRKREERMGEAHI*CRL 365
I+ + K+GKVIIID+ ++ D E E +L +D++M+ + GQ + E+ +
Sbjct: 288 ITSKDKKGKVIIIDMMMENQKGDEESIETQLFFDMLMMALVRGQERNEKEWAKLFTDAGF 347
Query: 364 PAATRVTPICGFKSLIEVFP 305
+ ++TPI G +SLIEV+P
Sbjct: 348 -SDYKITPILGLRSLIEVYP 366
>gb|AAB71213.1| methyltransferase [Prunus armeniaca]
Length = 354
Score = 139 bits (351), Expect = 3e-32
Identities = 64/140 (45%), Positives = 99/140 (70%)
Frame = -3
Query: 724 CTVLDQPQLVANLTGDENLNFVGGDMFKSVPPADAVLLKWVLHDWNDELSLKILKNCKEA 545
CTVLD P +VA+L G +NL +V GDMF++VP ADA+ LKW+LHDW+DE +KIL+ CK A
Sbjct: 216 CTVLDLPHVVADLKGSKNLEYVAGDMFEAVPAADAIFLKWILHDWSDEECVKILERCKAA 275
Query: 544 ISGRGKEGKVIIIDISIDETSDDRELTELKLDYDLVMLTMFNGQRKREERMGEAHI*CRL 365
++ GK+GKVII++++++ + D+E E +L +D+ M+ M G+ + E+ +
Sbjct: 276 VTREGKKGKVIIVEMTVENKNTDKESGETQLFFDMHMMVMSTGKERNEKEWAKLFSDAGF 335
Query: 364 PAATRVTPICGFKSLIEVFP 305
+ ++TP+ GFKSLIEV+P
Sbjct: 336 -SQYKITPLFGFKSLIEVYP 354
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 584,887,604
Number of Sequences: 1393205
Number of extensions: 12004370
Number of successful extensions: 31981
Number of sequences better than 10.0: 201
Number of HSP's better than 10.0 without gapping: 30409
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 31829
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 34062062287
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
