Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC001808A_C01 KMC001808A_c01
(573 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAC58013.1| S-adenosyl-L-methionine: 2,7,4'-trihydroxyisofla... 117 1e-27
dbj|BAC58012.1| S-adenosyl-L-methionine: daidzein 7-0-methyltran... 117 2e-27
pir||T09254 isoflavone-7-O-methyltransferase (EC 2.1.1.-) 9 - al... 116 2e-26
gb|AAC49926.1| 7-O-methyltransferase [Medicago sativa] 114 6e-26
pir||T06786 6a-hydroxymaackiain methyltransferase (EC 2.1.1.-) -... 109 2e-25
>dbj|BAC58013.1| S-adenosyl-L-methionine: 2,7,4'-trihydroxyisoflavanone
4'-O-methyltransferase [Lotus japonicus]
Length = 365
Score = 117 bits (292), Expect(2) = 1e-27
Identities = 61/107 (57%), Positives = 78/107 (72%), Gaps = 1/107 (0%)
Frame = -1
Query: 534 PEADAVLLKWVLHDWSDEVCIQILKNCKDSVSRNGNRGKVIIMDIIINEKEDTQAMTQTK 355
P ADAVLLKWVLHDW+DE+ ++ILKNCK+++S G GKVII+DI I+E D + +T+ K
Sbjct: 257 PPADAVLLKWVLHDWNDELSLKILKNCKEAISGRGKEGKVIIIDISIDETSDDRELTELK 316
Query: 354 LCMDLIMMGI-NGKERTGKEWKHLIQGSTGFKDYKILYPSPGFRSLI 217
L DL+M+ + NGKER KEW+ LI GF YKI P GF+SLI
Sbjct: 317 LDYDLVMLTMFNGKEREKKEWEKLIY-DAGFSSYKIT-PICGFKSLI 361
Score = 28.1 bits (61), Expect(2) = 1e-27
Identities = 11/13 (84%), Positives = 13/13 (99%)
Frame = -3
Query: 571 LSFVGGDMFKSMP 533
L+FVGGDMFKS+P
Sbjct: 245 LNFVGGDMFKSVP 257
>dbj|BAC58012.1| S-adenosyl-L-methionine: daidzein 7-0-methyltransferase
[Glycyrrhiza echinata]
Length = 357
Score = 117 bits (294), Expect(2) = 2e-27
Identities = 58/106 (54%), Positives = 79/106 (73%)
Frame = -1
Query: 534 PEADAVLLKWVLHDWSDEVCIQILKNCKDSVSRNGNRGKVIIMDIIINEKEDTQAMTQTK 355
P+ADAVLLKW+LH+W+D C +IL+ CK++VS +G +GKVII++++INE +D +T TK
Sbjct: 250 PKADAVLLKWILHNWTDNDCRRILEKCKEAVSSDGEKGKVIIIEMVINENQDEHEITGTK 309
Query: 354 LCMDLIMMGINGKERTGKEWKHLIQGSTGFKDYKILYPSPGFRSLI 217
L MD+ M +NGKER+ +EWK L GF+DYKI P GF SLI
Sbjct: 310 LLMDVNMACLNGKERSEEEWKKLFI-EAGFRDYKI-SPLTGFLSLI 353
Score = 26.6 bits (57), Expect(2) = 2e-27
Identities = 10/13 (76%), Positives = 13/13 (99%)
Frame = -3
Query: 571 LSFVGGDMFKSMP 533
LS+VGGDMF+S+P
Sbjct: 238 LSYVGGDMFQSVP 250
>pir||T09254 isoflavone-7-O-methyltransferase (EC 2.1.1.-) 9 - alfalfa
gi|2580584|gb|AAC49927.1| 7-O-methyltransferase
[Medicago sativa]
Length = 352
Score = 116 bits (290), Expect(2) = 2e-26
Identities = 58/106 (54%), Positives = 78/106 (72%)
Frame = -1
Query: 534 PEADAVLLKWVLHDWSDEVCIQILKNCKDSVSRNGNRGKVIIMDIIINEKEDTQAMTQTK 355
P ADAVLLK++LH+W+D+ C++ILK CK++V+ +G RGKV I+D++INEK+D +TQ K
Sbjct: 245 PNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVINEKKDENQVTQIK 304
Query: 354 LCMDLIMMGINGKERTGKEWKHLIQGSTGFKDYKILYPSPGFRSLI 217
L MD+ M +NGKER +EWK L GF+ YKI P GF SLI
Sbjct: 305 LLMDVNMACLNGKERNEEEWKKLFI-EAGFQHYKI-SPLTGFLSLI 348
Score = 24.6 bits (52), Expect(2) = 2e-26
Identities = 9/13 (69%), Positives = 12/13 (92%)
Frame = -3
Query: 571 LSFVGGDMFKSMP 533
L++VGGDMF S+P
Sbjct: 233 LTYVGGDMFTSIP 245
>gb|AAC49926.1| 7-O-methyltransferase [Medicago sativa]
Length = 352
Score = 114 bits (285), Expect(2) = 6e-26
Identities = 57/106 (53%), Positives = 78/106 (72%)
Frame = -1
Query: 534 PEADAVLLKWVLHDWSDEVCIQILKNCKDSVSRNGNRGKVIIMDIIINEKEDTQAMTQTK 355
P ADAVLLK++LH+W+D+ C++ILK CK++V+ +G RGKV I+D++I+EK+D +TQ K
Sbjct: 245 PNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVIDEKKDENQVTQIK 304
Query: 354 LCMDLIMMGINGKERTGKEWKHLIQGSTGFKDYKILYPSPGFRSLI 217
L MD+ M +NGKER +EWK L GF+ YKI P GF SLI
Sbjct: 305 LLMDVNMACLNGKERNEEEWKKLFI-EAGFQHYKI-SPLTGFLSLI 348
Score = 24.6 bits (52), Expect(2) = 6e-26
Identities = 9/13 (69%), Positives = 12/13 (92%)
Frame = -3
Query: 571 LSFVGGDMFKSMP 533
L++VGGDMF S+P
Sbjct: 233 LTYVGGDMFTSIP 245
>pir||T06786 6a-hydroxymaackiain methyltransferase (EC 2.1.1.-) - garden pea
gi|1568637|gb|AAC49856.1| 6a-hydroxymaackiain
methyltransferase [Pisum sativum]
Length = 360
Score = 109 bits (272), Expect(2) = 2e-25
Identities = 58/107 (54%), Positives = 77/107 (71%), Gaps = 1/107 (0%)
Frame = -1
Query: 534 PEADAVLLKWVLHDWSDEVCIQILKNCKDSVSRNGNRGKVIIMDIIINEKEDTQAMTQTK 355
P ADAVLLKWVLHDW+DE+ ++ILKN K+++S G GKVII+DI I+E D + +T+ +
Sbjct: 252 PSADAVLLKWVLHDWNDELSLKILKNSKEAISHKGKDGKVIIIDISIDENSDDRGLTELQ 311
Query: 354 LCMDLIMMGI-NGKERTGKEWKHLIQGSTGFKDYKILYPSPGFRSLI 217
L D++M+ + GKERT KEW+ LI GF YKI P GF+SLI
Sbjct: 312 LEYDVVMLTMFLGKERTKKEWEKLIY-DAGFSRYKIT-PICGFKSLI 356
Score = 28.1 bits (61), Expect(2) = 2e-25
Identities = 11/13 (84%), Positives = 13/13 (99%)
Frame = -3
Query: 571 LSFVGGDMFKSMP 533
L+FVGGDMFKS+P
Sbjct: 240 LNFVGGDMFKSVP 252
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 446,294,039
Number of Sequences: 1393205
Number of extensions: 9140776
Number of successful extensions: 17760
Number of sequences better than 10.0: 190
Number of HSP's better than 10.0 without gapping: 17242
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 17677
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 21243732558
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
