Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC001525A_C01 KMC001525A_c01
(785 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|O23787|THI4_CITSI Thiazole biosynthetic enzyme, chloroplast p... 315 1e-90
dbj|BAA88228.1| thiamin biosynthetic enzyme [Glycine max] 312 3e-90
dbj|BAA88226.1| thiamin biosynthetic enzyme [Glycine max] 310 1e-89
dbj|BAA88227.1| thiamin biosynthetic enzyme [Glycine max] 308 1e-88
dbj|BAA88225.1| thiamin biosynthetic enzyme [Glycine max] 306 3e-88
>sp|O23787|THI4_CITSI Thiazole biosynthetic enzyme, chloroplast precursor
gi|7446894|pir||T10474 thiamin biosynthesis protein thi1
- sweet orange gi|2582665|emb|CAB05370.1| thi [Citrus
sinensis]
Length = 356
Score = 315 bits (808), Expect(3) = 1e-90
Identities = 153/165 (92%), Positives = 164/165 (98%)
Frame = +1
Query: 256 RQSIVSREMTRRYMTDMVTFADTDVVIVGAGSAGLTCAYELSKNPNVQVAIIEQSVSPGG 435
++SIVSREMTRRYMTDM+T+ADTDVV+VGAGSAGL+CAYELSKNPN+Q+AIIEQSVSPGG
Sbjct: 69 KESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQIAIIEQSVSPGG 128
Query: 436 GAWLGGQLFSAMVVRKPAHLFLNELGVDYDEQDNYVVIKHAALFTSTIMSKLLARPNVKL 615
GAWLGGQLFSAMVVRKPAH+FL+ELG+DYDEQDNYVVIKHAALFTSTIMSKLLARPNVKL
Sbjct: 129 GAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTIMSKLLARPNVKL 188
Query: 616 FNAVAAEDLIVKNGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKV 750
FNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKV
Sbjct: 189 FNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKV 233
Score = 32.0 bits (71), Expect(3) = 1e-90
Identities = 15/28 (53%), Positives = 18/28 (63%)
Frame = +3
Query: 174 SACPWTASSAPSPPPPYDLGSFKFAPIK 257
S+ P S + S PPYDL +FKF PIK
Sbjct: 42 SSRPNNLSISASASPPYDLNTFKFDPIK 69
Score = 30.0 bits (66), Expect(3) = 1e-90
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = +2
Query: 752 VSSCGHDGPFG 784
VSSCGHDGPFG
Sbjct: 235 VSSCGHDGPFG 245
>dbj|BAA88228.1| thiamin biosynthetic enzyme [Glycine max]
Length = 349
Score = 312 bits (799), Expect(3) = 3e-90
Identities = 153/165 (92%), Positives = 163/165 (98%)
Frame = +1
Query: 256 RQSIVSREMTRRYMTDMVTFADTDVVIVGAGSAGLTCAYELSKNPNVQVAIIEQSVSPGG 435
R+SIVSREMTRRYMTDMVT ADTDVV+VGAGSAGL+CAYELSKNP+V +AI+EQS+SPGG
Sbjct: 64 RESIVSREMTRRYMTDMVTHADTDVVVVGAGSAGLSCAYELSKNPSVNIAIVEQSISPGG 123
Query: 436 GAWLGGQLFSAMVVRKPAHLFLNELGVDYDEQDNYVVIKHAALFTSTIMSKLLARPNVKL 615
GAWLGGQLFSAMVVRKPAHLFL+ELG++YDEQDNYVVIKHAALFTSTIMSKLLARPNVKL
Sbjct: 124 GAWLGGQLFSAMVVRKPAHLFLDELGLEYDEQDNYVVIKHAALFTSTIMSKLLARPNVKL 183
Query: 616 FNAVAAEDLIVKNGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKV 750
FNAVAAEDLIVKNGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKV
Sbjct: 184 FNAVAAEDLIVKNGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKV 228
Score = 34.3 bits (77), Expect(3) = 3e-90
Identities = 13/22 (59%), Positives = 17/22 (77%)
Frame = +3
Query: 192 ASSAPSPPPPYDLGSFKFAPIK 257
AS + + PPPYD GSF+F PI+
Sbjct: 43 ASMSAAAPPPYDFGSFRFEPIR 64
Score = 30.0 bits (66), Expect(3) = 3e-90
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = +2
Query: 752 VSSCGHDGPFG 784
VSSCGHDGPFG
Sbjct: 230 VSSCGHDGPFG 240
>dbj|BAA88226.1| thiamin biosynthetic enzyme [Glycine max]
Length = 349
Score = 310 bits (794), Expect(3) = 1e-89
Identities = 153/165 (92%), Positives = 162/165 (97%)
Frame = +1
Query: 256 RQSIVSREMTRRYMTDMVTFADTDVVIVGAGSAGLTCAYELSKNPNVQVAIIEQSVSPGG 435
R+SIVSREMTRRYMTDMVT ADTDVV+VGAGSAGL+CAYELSKNP+V +AI+EQSVSPGG
Sbjct: 64 RESIVSREMTRRYMTDMVTHADTDVVVVGAGSAGLSCAYELSKNPSVNIAIVEQSVSPGG 123
Query: 436 GAWLGGQLFSAMVVRKPAHLFLNELGVDYDEQDNYVVIKHAALFTSTIMSKLLARPNVKL 615
GAWLGGQLFSAMVVRKPAHLFL+ELG++YDEQDNYVVIKHAAL TSTIMSKLLARPNVKL
Sbjct: 124 GAWLGGQLFSAMVVRKPAHLFLDELGLEYDEQDNYVVIKHAALLTSTIMSKLLARPNVKL 183
Query: 616 FNAVAAEDLIVKNGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKV 750
FNAVAAEDLIVKNGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKV
Sbjct: 184 FNAVAAEDLIVKNGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKV 228
Score = 34.3 bits (77), Expect(3) = 1e-89
Identities = 13/22 (59%), Positives = 17/22 (77%)
Frame = +3
Query: 192 ASSAPSPPPPYDLGSFKFAPIK 257
AS + + PPPYD GSF+F PI+
Sbjct: 43 ASMSAAAPPPYDFGSFRFEPIR 64
Score = 30.0 bits (66), Expect(3) = 1e-89
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = +2
Query: 752 VSSCGHDGPFG 784
VSSCGHDGPFG
Sbjct: 230 VSSCGHDGPFG 240
>dbj|BAA88227.1| thiamin biosynthetic enzyme [Glycine max]
Length = 345
Score = 308 bits (788), Expect(3) = 1e-88
Identities = 152/165 (92%), Positives = 161/165 (97%)
Frame = +1
Query: 256 RQSIVSREMTRRYMTDMVTFADTDVVIVGAGSAGLTCAYELSKNPNVQVAIIEQSVSPGG 435
R+SIVSREMTRRYM DMVT ADTDVVIVGAGSAGL+CAYELSKNP++ +AI+EQSVSPGG
Sbjct: 60 RESIVSREMTRRYMIDMVTHADTDVVIVGAGSAGLSCAYELSKNPSINIAIVEQSVSPGG 119
Query: 436 GAWLGGQLFSAMVVRKPAHLFLNELGVDYDEQDNYVVIKHAALFTSTIMSKLLARPNVKL 615
GAWLGGQLFSAMVVRKPAHLFL+EL V+YDEQDNYVVIKHAALFTSTIMSKLLARPNVKL
Sbjct: 120 GAWLGGQLFSAMVVRKPAHLFLDELNVEYDEQDNYVVIKHAALFTSTIMSKLLARPNVKL 179
Query: 616 FNAVAAEDLIVKNGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKV 750
FNAVAAEDLIVKNGRVGGVVTNWALVS+NHDTQSCMDPNVMEAKV
Sbjct: 180 FNAVAAEDLIVKNGRVGGVVTNWALVSLNHDTQSCMDPNVMEAKV 224
Score = 33.1 bits (74), Expect(3) = 1e-88
Identities = 18/48 (37%), Positives = 27/48 (55%), Gaps = 1/48 (2%)
Frame = +3
Query: 117 SPPSTNHSPSNP*NLH*GSSACPWTA-SSAPSPPPPYDLGSFKFAPIK 257
SPPS+ + S+ + H + P +S + PPYD GSF+F PI+
Sbjct: 13 SPPSSLFNKSSSPSFHATPTLRPLAPRASMSASAPPYDFGSFRFDPIR 60
Score = 30.0 bits (66), Expect(3) = 1e-88
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = +2
Query: 752 VSSCGHDGPFG 784
VSSCGHDGPFG
Sbjct: 226 VSSCGHDGPFG 236
>dbj|BAA88225.1| thiamin biosynthetic enzyme [Glycine max]
Length = 345
Score = 306 bits (785), Expect(3) = 3e-88
Identities = 151/165 (91%), Positives = 161/165 (97%)
Frame = +1
Query: 256 RQSIVSREMTRRYMTDMVTFADTDVVIVGAGSAGLTCAYELSKNPNVQVAIIEQSVSPGG 435
R+SIVSREMTRRYM DMVT ADTDVVIVGAGSAGL+CAYELSKNP++ +AI+EQSVSPGG
Sbjct: 60 RESIVSREMTRRYMIDMVTHADTDVVIVGAGSAGLSCAYELSKNPSINIAIVEQSVSPGG 119
Query: 436 GAWLGGQLFSAMVVRKPAHLFLNELGVDYDEQDNYVVIKHAALFTSTIMSKLLARPNVKL 615
GAWLGGQLFSAMVVRKPAHLFL+EL ++YDEQDNYVVIKHAALFTSTIMSKLLARPNVKL
Sbjct: 120 GAWLGGQLFSAMVVRKPAHLFLDELNLEYDEQDNYVVIKHAALFTSTIMSKLLARPNVKL 179
Query: 616 FNAVAAEDLIVKNGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKV 750
FNAVAAEDLIVKNGRVGGVVTNWALVS+NHDTQSCMDPNVMEAKV
Sbjct: 180 FNAVAAEDLIVKNGRVGGVVTNWALVSLNHDTQSCMDPNVMEAKV 224
Score = 33.1 bits (74), Expect(3) = 3e-88
Identities = 18/48 (37%), Positives = 27/48 (55%), Gaps = 1/48 (2%)
Frame = +3
Query: 117 SPPSTNHSPSNP*NLH*GSSACPWTA-SSAPSPPPPYDLGSFKFAPIK 257
SPPS+ + S+ + H + P +S + PPYD GSF+F PI+
Sbjct: 13 SPPSSLFNKSSSPSFHATPTLRPLAPRASMSASAPPYDFGSFRFDPIR 60
Score = 30.0 bits (66), Expect(3) = 3e-88
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = +2
Query: 752 VSSCGHDGPFG 784
VSSCGHDGPFG
Sbjct: 226 VSSCGHDGPFG 236
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 859,571,862
Number of Sequences: 1393205
Number of extensions: 25684377
Number of successful extensions: 658555
Number of sequences better than 10.0: 7569
Number of HSP's better than 10.0 without gapping: 135000
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 358463
length of database: 448,689,247
effective HSP length: 121
effective length of database: 280,111,442
effective search space used: 39215601880
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
