Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC001485A_C01 KMC001485A_c01
(1092 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAL54888.1|AF116915_1 obtusifoliol-14-demethylase [Nicotiana ... 336 e-116
gb|AAL40888.1| obtusifoliol-14-demethylase [Nicotiana tabacum] 333 e-116
ref|NP_172633.1| putative obtusifoliol 14-alpha demethylase; pro... 323 e-113
gb|AAO16695.1| cytochrome P450-like protein [Sorghum bicolor] 326 e-112
sp|P93846|CP51_SORBI Cytochrome P450 51 (CYPL1) (P450-L1A1) (Obt... 326 e-112
>gb|AAL54888.1|AF116915_1 obtusifoliol-14-demethylase [Nicotiana tabacum]
Length = 487
Score = 336 bits (861), Expect(2) = e-116
Identities = 156/181 (86%), Positives = 173/181 (95%)
Frame = -2
Query: 1082 KSEDDMLQCFIDSKYKDGRPTTEGEVTGLLIAALFAGQHTSSITSTWTGAYLMCNNKYLS 903
K+E DMLQCFIDSKYKDGR TTE E+TGLLIAALFAGQHTSSITSTW GAYL+CNNKY+S
Sbjct: 250 KAESDMLQCFIDSKYKDGRATTESEITGLLIAALFAGQHTSSITSTWAGAYLLCNNKYMS 309
Query: 902 AVVEEQKVLMEKHGDRVDHDVLAEMDVLYRCIKEALRLHPPLIMLLRSSHSDFSVTTREG 723
AVV+EQK LM+KHG++VDHD+L+EM+VLYRCIKEALRLHPPLIMLLRSSHSDF+V TREG
Sbjct: 310 AVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHSDFTVKTREG 369
Query: 722 KEYDIPKGHIVATSPAFANRLPHIFKDPDTYDPDRFAVGREEDQAAGAFSYISFGGGRHG 543
KEYDIPKGHIVATSPAFANRLPH++K+PDTYDPDRFA GR+ED+ AGAFSYISFGGGRHG
Sbjct: 370 KEYDIPKGHIVATSPAFANRLPHVYKNPDTYDPDRFAPGRDEDKVAGAFSYISFGGGRHG 429
Query: 542 C 540
C
Sbjct: 430 C 430
Score = 107 bits (267), Expect(2) = e-116
Identities = 50/61 (81%), Positives = 54/61 (87%)
Frame = -3
Query: 556 GADMDALGEPFAYLQIKAIWTHLLRNFELELVSPFPEIDWNALVVGVKGKVMVRYKRRVL 377
G LGEPFAYLQIKAIW+HLLRNFE EL+SPFPEIDWNA+VVGVKGKVMV+YKRR L
Sbjct: 425 GGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAMVVGVKGKVMVKYKRRKL 484
Query: 376 S 374
S
Sbjct: 485 S 485
>gb|AAL40888.1| obtusifoliol-14-demethylase [Nicotiana tabacum]
Length = 487
Score = 333 bits (855), Expect(2) = e-116
Identities = 154/181 (85%), Positives = 172/181 (94%)
Frame = -2
Query: 1082 KSEDDMLQCFIDSKYKDGRPTTEGEVTGLLIAALFAGQHTSSITSTWTGAYLMCNNKYLS 903
K+E DMLQCFIDSKYKDGR TT+ E+TGLLIAALFAGQHTSSITSTWTGAYL+CNNKY+S
Sbjct: 250 KAESDMLQCFIDSKYKDGRATTDSEITGLLIAALFAGQHTSSITSTWTGAYLLCNNKYMS 309
Query: 902 AVVEEQKVLMEKHGDRVDHDVLAEMDVLYRCIKEALRLHPPLIMLLRSSHSDFSVTTREG 723
AVV+EQK LM+KHG++VDHD+L+EM+VLYRCIKE LRLHPPLIMLLRSSHSDF+V TREG
Sbjct: 310 AVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEVLRLHPPLIMLLRSSHSDFTVKTREG 369
Query: 722 KEYDIPKGHIVATSPAFANRLPHIFKDPDTYDPDRFAVGREEDQAAGAFSYISFGGGRHG 543
KEYDIPKGHIVATSPAFANRLPH++K+PDTYDPDRF GR+ED+ AGAFSYISFGGGRHG
Sbjct: 370 KEYDIPKGHIVATSPAFANRLPHVYKNPDTYDPDRFTPGRDEDKVAGAFSYISFGGGRHG 429
Query: 542 C 540
C
Sbjct: 430 C 430
Score = 107 bits (267), Expect(2) = e-116
Identities = 50/61 (81%), Positives = 54/61 (87%)
Frame = -3
Query: 556 GADMDALGEPFAYLQIKAIWTHLLRNFELELVSPFPEIDWNALVVGVKGKVMVRYKRRVL 377
G LGEPFAYLQIKAIW+HLLRNFE EL+SPFPEIDWNA+VVGVKGKVMV+YKRR L
Sbjct: 425 GGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAMVVGVKGKVMVKYKRRKL 484
Query: 376 S 374
S
Sbjct: 485 S 485
>ref|NP_172633.1| putative obtusifoliol 14-alpha demethylase; protein id: At1g11680.1,
supported by cDNA: 110306., supported by cDNA:
gi_14624982, supported by cDNA: gi_15292852, supported by
cDNA: gi_15294293, supported by cDNA: gi_20258896
[Arabidopsis thaliana] gi|25282616|pir||D86250
hypothetical protein [imported] - Arabidopsis thaliana
gi|4835788|gb|AAD30254.1|AC007296_15 Strong similarity to
gb|U74319 obtusifoliol 14-alpha demethylase (CYP51) from
Sorghum bicolor and is a member of the PF|00067
cytochrome P450 family. ESTs gb|AA72030, gb|N65031 and
gb|AA651059 come from this gene. [Arabidopsis thaliana]
gi|14624983|dbj|BAB61873.1| obtusifoliol 14-demethylase
[Arabidopsis thaliana] gi|15292853|gb|AAK92797.1|
putative obtusifoliol 14-alpha demethylase [Arabidopsis
thaliana] gi|15294294|gb|AAK95324.1|AF410338_1
At1g11680/F25C20_17 [Arabidopsis thaliana]
gi|20258897|gb|AAM14142.1| putative obtusifoliol 14-alpha
demethylase [Arabidopsis thaliana]
gi|21536753|gb|AAM61085.1| putative obtusifoliol 14-alpha
demethylase [Arabidopsis thaliana]
Length = 488
Score = 323 bits (827), Expect(2) = e-113
Identities = 151/183 (82%), Positives = 170/183 (92%)
Frame = -2
Query: 1088 ANKSEDDMLQCFIDSKYKDGRPTTEGEVTGLLIAALFAGQHTSSITSTWTGAYLMCNNKY 909
+ K+E+DMLQCFI+SKYKDGR TTE EVTGLLIAALFAGQHTSSITSTWTGAYLM +Y
Sbjct: 251 SGKTENDMLQCFIESKYKDGRQTTESEVTGLLIAALFAGQHTSSITSTWTGAYLMRYKEY 310
Query: 908 LSAVVEEQKVLMEKHGDRVDHDVLAEMDVLYRCIKEALRLHPPLIMLLRSSHSDFSVTTR 729
SA ++EQK L+ KHGD++DHD+L+EMDVLYRCIKEALRLHPPLIML+R+SHSDFSVT R
Sbjct: 311 FSAALDEQKNLIAKHGDKIDHDILSEMDVLYRCIKEALRLHPPLIMLMRASHSDFSVTAR 370
Query: 728 EGKEYDIPKGHIVATSPAFANRLPHIFKDPDTYDPDRFAVGREEDQAAGAFSYISFGGGR 549
+GK YDIPKGHIVATSPAFANRLPHIFKDPDTYDP+RF+ GREED+AAGAFSYI+FGGGR
Sbjct: 371 DGKTYDIPKGHIVATSPAFANRLPHIFKDPDTYDPERFSPGREEDKAAGAFSYIAFGGGR 430
Query: 548 HGC 540
HGC
Sbjct: 431 HGC 433
Score = 108 bits (270), Expect(2) = e-113
Identities = 52/61 (85%), Positives = 54/61 (88%)
Frame = -3
Query: 556 GADMDALGEPFAYLQIKAIWTHLLRNFELELVSPFPEIDWNALVVGVKGKVMVRYKRRVL 377
G LGEPFAYLQIKAIW+HLLRNFELELVSPFPEIDWNA+VVGVKG VMVRYKRR L
Sbjct: 428 GGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNAMVVGVKGNVMVRYKRRQL 487
Query: 376 S 374
S
Sbjct: 488 S 488
>gb|AAO16695.1| cytochrome P450-like protein [Sorghum bicolor]
Length = 492
Score = 326 bits (836), Expect(2) = e-112
Identities = 151/184 (82%), Positives = 173/184 (93%)
Frame = -2
Query: 1091 SANKSEDDMLQCFIDSKYKDGRPTTEGEVTGLLIAALFAGQHTSSITSTWTGAYLMCNNK 912
++ +SE+DMLQCFIDSKYK+GRPTTEGEVTGLLIAALFAGQHTSSITSTWTGAY++ +
Sbjct: 251 ASGQSEEDMLQCFIDSKYKNGRPTTEGEVTGLLIAALFAGQHTSSITSTWTGAYMLRFKQ 310
Query: 911 YLSAVVEEQKVLMEKHGDRVDHDVLAEMDVLYRCIKEALRLHPPLIMLLRSSHSDFSVTT 732
Y + VEEQK +M++HGD++DHD+LAEMDVLYRCIKEALRLHPPLIMLLR SHSDF+VTT
Sbjct: 311 YFAEAVEEQKDVMKRHGDKIDHDILAEMDVLYRCIKEALRLHPPLIMLLRQSHSDFTVTT 370
Query: 731 REGKEYDIPKGHIVATSPAFANRLPHIFKDPDTYDPDRFAVGREEDQAAGAFSYISFGGG 552
+EGKEYDIPKGHIVATSP+FANRLPHI+K+PD+YDPDRF GREED+AAGAFSYISFGGG
Sbjct: 371 KEGKEYDIPKGHIVATSPSFANRLPHIYKNPDSYDPDRFGPGREEDKAAGAFSYISFGGG 430
Query: 551 RHGC 540
RHGC
Sbjct: 431 RHGC 434
Score = 102 bits (254), Expect(2) = e-112
Identities = 48/60 (80%), Positives = 51/60 (85%)
Frame = -3
Query: 556 GADMDALGEPFAYLQIKAIWTHLLRNFELELVSPFPEIDWNALVVGVKGKVMVRYKRRVL 377
G LGEPFAYLQIKAIWTHLLRNFE ELVSPFPE DWNA+VVG+KG+VMV YKRR L
Sbjct: 429 GGRHGCLGEPFAYLQIKAIWTHLLRNFEFELVSPFPENDWNAMVVGIKGEVMVNYKRRKL 488
>sp|P93846|CP51_SORBI Cytochrome P450 51 (CYPL1) (P450-L1A1) (Obtusifoliol 14-alpha
demethylase) gi|7430692|pir||T14820 obtusifoliol 14-alpha
demethylase CYP51 - sorghum gi|1658193|gb|AAC49659.1|
obtusifoliol 14-alpha demethylase CYP51 [Sorghum bicolor]
Length = 492
Score = 326 bits (836), Expect(2) = e-112
Identities = 151/184 (82%), Positives = 173/184 (93%)
Frame = -2
Query: 1091 SANKSEDDMLQCFIDSKYKDGRPTTEGEVTGLLIAALFAGQHTSSITSTWTGAYLMCNNK 912
++ +SE+DMLQCFIDSKYK+GRPTTEGEVTGLLIAALFAGQHTSSITSTWTGAY++ +
Sbjct: 251 ASGQSEEDMLQCFIDSKYKNGRPTTEGEVTGLLIAALFAGQHTSSITSTWTGAYMLRFKQ 310
Query: 911 YLSAVVEEQKVLMEKHGDRVDHDVLAEMDVLYRCIKEALRLHPPLIMLLRSSHSDFSVTT 732
Y + VEEQK +M++HGD++DHD+LAEMDVLYRCIKEALRLHPPLIMLLR SHSDF+VTT
Sbjct: 311 YFAEAVEEQKDVMKRHGDKIDHDILAEMDVLYRCIKEALRLHPPLIMLLRQSHSDFTVTT 370
Query: 731 REGKEYDIPKGHIVATSPAFANRLPHIFKDPDTYDPDRFAVGREEDQAAGAFSYISFGGG 552
+EGKEYDIPKGHIVATSP+FANRLPHI+K+PD+YDPDRF GREED+AAGAFSYISFGGG
Sbjct: 371 KEGKEYDIPKGHIVATSPSFANRLPHIYKNPDSYDPDRFGPGREEDKAAGAFSYISFGGG 430
Query: 551 RHGC 540
RHGC
Sbjct: 431 RHGC 434
Score = 102 bits (254), Expect(2) = e-112
Identities = 48/60 (80%), Positives = 51/60 (85%)
Frame = -3
Query: 556 GADMDALGEPFAYLQIKAIWTHLLRNFELELVSPFPEIDWNALVVGVKGKVMVRYKRRVL 377
G LGEPFAYLQIKAIWTHLLRNFE ELVSPFPE DWNA+VVG+KG+VMV YKRR L
Sbjct: 429 GGRHGCLGEPFAYLQIKAIWTHLLRNFEFELVSPFPENDWNAMVVGIKGEVMVNYKRRKL 488
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,008,324,974
Number of Sequences: 1393205
Number of extensions: 24122878
Number of successful extensions: 76177
Number of sequences better than 10.0: 2140
Number of HSP's better than 10.0 without gapping: 69519
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 74348
length of database: 448,689,247
effective HSP length: 125
effective length of database: 274,538,622
effective search space used: 65340192036
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
