KMC001351A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC001351A_C01 KMC001351A_c01
ccctacataaaagcagacgacttgtatAGAATAATATAATATATATCGTACTATACAATC
TTATGACTTCAATTCATTAACCGATCCAAATATCTTTACAGCCTTAAACATCTAATCATT
ATAAATTAAAAATAAAATAAGATTAAACAAAATAAAGCTTCACATCACTTCATGCCCATT
GCACAAATCCATAATCATTTGATAAGTTATGAGACTCGTGACATTTTCCTAATCAATCAA
CATTTTTTCCCCATTTGGGGTCTGAATGGTGGCCTTGCTACTACTCTGTATGCTTAGCCC
TTCACCGTGTTTCTTCATTTCCTGAGACCTGACAAACTCCTCAATTCGTGCTTTTAGTTC
AACATCAGGAATCAGCATATCCGCAGTAAGATGAG


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001351A_C01 KMC001351A_c01
         (395 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_568313.1| ubiquitin-fusion degradation protein-like; prot...    58  2e-08
pir||T51518 ubiquitin-fusion degradation protein-like - Arabidop...    58  2e-08
ref|XP_137497.1| similar to NPC1 (Niemann-Pick disease, type C1,...    32  1.9
pir||T14576 nosA protein - slime mold (Dictyostelium discoideum)...    31  4.3
ref|NP_037521.1| NPC1 (Niemann-Pick disease, type C1, gene)-like...    31  4.3

>ref|NP_568313.1| ubiquitin-fusion degradation protein-like; protein id: At5g15400.1
            [Arabidopsis thaliana]
          Length = 1037

 Score = 58.2 bits (139), Expect = 2e-08
 Identities = 32/54 (59%), Positives = 40/54 (73%)
 Frame = -3

Query: 393  HLTADMLIPDVELKARIEEFVRSQEMKKHGEGLSIQSSSKATIQTPNGEKMLID 232
            HLT+DMLIPD+ELKA+I+EFV+S + KK   G    SS+K  IQT N + MLID
Sbjct: 987  HLTSDMLIPDIELKAKIDEFVKSHQSKKRTSG--EDSSNKERIQTTNSD-MLID 1037

>pir||T51518 ubiquitin-fusion degradation protein-like - Arabidopsis thaliana
            gi|9755795|emb|CAC01739.1| ubiquitin-fusion degradation
            protein-like [Arabidopsis thaliana]
          Length = 1038

 Score = 58.2 bits (139), Expect = 2e-08
 Identities = 32/54 (59%), Positives = 40/54 (73%)
 Frame = -3

Query: 393  HLTADMLIPDVELKARIEEFVRSQEMKKHGEGLSIQSSSKATIQTPNGEKMLID 232
            HLT+DMLIPD+ELKA+I+EFV+S + KK   G    SS+K  IQT N + MLID
Sbjct: 988  HLTSDMLIPDIELKAKIDEFVKSHQSKKRTSG--EDSSNKERIQTTNSD-MLID 1038

>ref|XP_137497.1| similar to NPC1 (Niemann-Pick disease, type C1, gene)-like 1 [Homo
           sapiens] [Mus musculus] gi|25056582|ref|XP_204570.1|
           similar to NPC1 (Niemann-Pick disease, type C1,
           gene)-like 1 [Homo sapiens] [Mus musculus]
          Length = 1333

 Score = 32.0 bits (71), Expect = 1.9
 Identities = 14/34 (41%), Positives = 23/34 (67%)
 Frame = +2

Query: 278 LLLCMLSPSPCFFIS*DLTNSSIRAFSSTSGISI 379
           +LLC LS + CFF+    +  ++R F+ TSG++I
Sbjct: 747 MLLCSLSEAICFFLGALTSMPAVRTFALTSGLAI 780

>pir||T14576 nosA protein - slime mold (Dictyostelium discoideum)
            gi|2827469|gb|AAC34746.1| NOSA [Dictyostelium discoideum]
          Length = 1089

 Score = 30.8 bits (68), Expect = 4.3
 Identities = 13/25 (52%), Positives = 20/25 (80%)
 Frame = -3

Query: 393  HLTADMLIPDVELKARIEEFVRSQE 319
            HLT +MLI DVE K +I+E++ S++
Sbjct: 1063 HLTPEMLIDDVETKKKIDEWLASKK 1087

>ref|NP_037521.1| NPC1 (Niemann-Pick disease, type C1, gene)-like 1 [Homo sapiens]
           gi|6643036|gb|AAF20396.1|AF192522_1 Niemann-Pick C3
           protein; NPC3 [Homo sapiens]
          Length = 1359

 Score = 30.8 bits (68), Expect = 4.3
 Identities = 13/34 (38%), Positives = 22/34 (64%)
 Frame = +2

Query: 278 LLLCMLSPSPCFFIS*DLTNSSIRAFSSTSGISI 379
           +LLC LS + CFF+       ++R F+ TSG+++
Sbjct: 747 MLLCSLSEAICFFLGALTPMPAVRTFALTSGLAV 780

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 291,735,236
Number of Sequences: 1393205
Number of extensions: 5447883
Number of successful extensions: 10070
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 9921
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 10069
length of database: 448,689,247
effective HSP length: 107
effective length of database: 299,616,312
effective search space used: 7190791488
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 MWM248h05_f AV768545 1 364
2 MPDL008b12_f AV776908 28 396
3 GENLf078b04 BP066578 61 346




Lotus japonicus
Kazusa DNA Research Institute