Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC001128A_C01 KMC001128A_c01
(660 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAD10241.1| histidyl-tRNA synthetase [Triticum aestivum] 78 1e-19
gb|AAD10242.1| histidyl-tRNA synthetase [Triticum aestivum] 78 1e-19
ref|NP_186925.1| putative histidyl tRNA synthetase; protein id: ... 72 4e-16
sp|P93422|SYH_ORYSA HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LI... 73 4e-12
dbj|BAC40595.1| unnamed protein product [Mus musculus] 38 9e-06
>gb|AAD10241.1| histidyl-tRNA synthetase [Triticum aestivum]
Length = 479
Score = 78.2 bits (191), Expect(2) = 1e-19
Identities = 37/63 (58%), Positives = 45/63 (70%)
Frame = -1
Query: 660 QKDQNQLARPTQTEVLVSILGNDLTLAAELTGELWDAGVDAEFLVNKRRQKHFDYAKASR 481
+K++NQ+ R T+TEVLVSILG DL LAAEL ELW AG+ AEF + R Q H YA +
Sbjct: 374 EKEKNQVIRATETEVLVSILGKDLILAAELVNELWSAGIKAEFKLTTRVQNHIKYATQTG 433
Query: 480 IPW 472
IPW
Sbjct: 434 IPW 436
Score = 40.4 bits (93), Expect(2) = 1e-19
Identities = 17/40 (42%), Positives = 30/40 (74%)
Frame = -2
Query: 470 LVLVGEKEIKEGIVLLKNLEAHHDVSIPRGSFVEEVRKQL 351
+VLVGE E+++G V LK++ A+ + +PR FVE ++++L
Sbjct: 437 MVLVGESELRDGKVKLKDIRANQEEEVPRKDFVEVLKQRL 476
>gb|AAD10242.1| histidyl-tRNA synthetase [Triticum aestivum]
Length = 432
Score = 78.2 bits (191), Expect(2) = 1e-19
Identities = 37/63 (58%), Positives = 45/63 (70%)
Frame = -1
Query: 660 QKDQNQLARPTQTEVLVSILGNDLTLAAELTGELWDAGVDAEFLVNKRRQKHFDYAKASR 481
+K++NQ+ R T+TEVLVSILG DL LAAEL ELW AG+ AEF + R Q H YA +
Sbjct: 327 EKEKNQVIRATETEVLVSILGKDLILAAELVNELWSAGIKAEFKLTTRVQNHIKYATQTG 386
Query: 480 IPW 472
IPW
Sbjct: 387 IPW 389
Score = 40.4 bits (93), Expect(2) = 1e-19
Identities = 17/40 (42%), Positives = 30/40 (74%)
Frame = -2
Query: 470 LVLVGEKEIKEGIVLLKNLEAHHDVSIPRGSFVEEVRKQL 351
+VLVGE E+++G V LK++ A+ + +PR FVE ++++L
Sbjct: 390 MVLVGESELRDGKVKLKDIRANQEEEVPRKDFVEVLKQRL 429
>ref|NP_186925.1| putative histidyl tRNA synthetase; protein id: At3g02760.1
[Arabidopsis thaliana]
gi|6728986|gb|AAF26984.1|AC018363_29 putative histidyl
tRNA synthetase [Arabidopsis thaliana]
Length = 479
Score = 72.0 bits (175), Expect(2) = 4e-16
Identities = 35/61 (57%), Positives = 47/61 (76%), Gaps = 1/61 (1%)
Frame = -1
Query: 651 QNQLARPTQTEVLVSIL-GNDLTLAAELTGELWDAGVDAEFLVNKRRQKHFDYAKASRIP 475
Q Q+ RPT+T+VLVSI+ N L AAEL +LW A ++AE+LV+KR++KHF+ AK S IP
Sbjct: 375 QKQVIRPTETQVLVSIMVDNKLAEAAELVSQLWGAKINAEYLVSKRKEKHFNRAKESGIP 434
Query: 474 W 472
W
Sbjct: 435 W 435
Score = 34.3 bits (77), Expect(2) = 4e-16
Identities = 21/51 (41%), Positives = 27/51 (52%), Gaps = 4/51 (7%)
Frame = -2
Query: 491 KHQGSLGLVLVGEKEIKEGIVLLKNL----EAHHDVSIPRGSFVEEVRKQL 351
K G +V+VGEKE+ V LK L E D + R FVEE++K L
Sbjct: 429 KESGIPWMVMVGEKELSGSFVTLKKLEKGSEEKEDQTCTRDRFVEELKKLL 479
>sp|P93422|SYH_ORYSA HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
gi|7437810|pir||T03774 probable histidine-tRNA ligase
(EC 6.1.1.21) - rice gi|1841704|emb|CAB06653.1| histidyl
tRNA Synthetase [Oryza sativa (japonica cultivar-group)]
gi|12083526|gb|AAG48835.1|AC084218_5 similar to Oryza
sativa histidtl-tRNA synthetase (T03774)
Length = 494
Score = 72.8 bits (177), Expect = 4e-12
Identities = 36/63 (57%), Positives = 45/63 (71%)
Frame = -1
Query: 660 QKDQNQLARPTQTEVLVSILGNDLTLAAELTGELWDAGVDAEFLVNKRRQKHFDYAKASR 481
+ ++NQ+ R T+TEVLVSI+G DL LAAEL ELW+AG+ AEF + R Q H YA S
Sbjct: 390 EMEKNQI-RATETEVLVSIIGKDLILAAELVSELWNAGIKAEFKLTTRIQNHLKYATQSG 448
Query: 480 IPW 472
IPW
Sbjct: 449 IPW 451
Score = 35.8 bits (81), Expect = 0.52
Identities = 22/62 (35%), Positives = 32/62 (51%), Gaps = 6/62 (9%)
Frame = -2
Query: 518 EDRSTLTMQKH------QGSLGLVLVGEKEIKEGIVLLKNLEAHHDVSIPRGSFVEEVRK 357
E + T +Q H G +VLVGE EI G V LKNL A + + R F + +++
Sbjct: 430 EFKLTTRIQNHLKYATQSGIPWMVLVGESEISSGKVKLKNLAASQEEEVDRTEFAQVLKQ 489
Query: 356 QL 351
+L
Sbjct: 490 KL 491
>dbj|BAC40595.1| unnamed protein product [Mus musculus]
Length = 509
Score = 38.1 bits (87), Expect(2) = 9e-06
Identities = 13/39 (33%), Positives = 29/39 (74%)
Frame = -2
Query: 470 LVLVGEKEIKEGIVLLKNLEAHHDVSIPRGSFVEEVRKQ 354
+ ++GE+E+K+G+V L+++ + +V + R VEE+R++
Sbjct: 463 VAIIGEQELKDGVVKLRSVASREEVDVQREDLVEEIRRR 501
Score = 33.1 bits (74), Expect(2) = 9e-06
Identities = 21/57 (36%), Positives = 29/57 (50%), Gaps = 3/57 (5%)
Frame = -1
Query: 636 RPTQTEVLVSILGNDLTLAA-ELTGELWDAGVDAEFLVNKRRQ--KHFDYAKASRIP 475
R T+T+VLV+ L +L ELWDAG+ AE L K + Y + + IP
Sbjct: 405 RTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIP 461
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 562,903,177
Number of Sequences: 1393205
Number of extensions: 12091037
Number of successful extensions: 33300
Number of sequences better than 10.0: 37
Number of HSP's better than 10.0 without gapping: 31788
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 33285
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 28289785200
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
