Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000986A_C01 KMC000986A_c01
(649 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_174475.1| G-protein, putative; protein id: At1g31930.1 [A... 224 1e-57
dbj|BAA93022.1| ESTs C74776(E51022),C26123(C116681) correspond t... 212 4e-54
gb|AAL34942.1|AC079037_15 Putative G-protein [Oryza sativa] 170 2e-41
pir||T01135 probable GTP-binding protein (extra large) [imported... 128 6e-29
ref|NP_565553.1| putative GTP-binding protein (extra large); pro... 128 6e-29
>ref|NP_174475.1| G-protein, putative; protein id: At1g31930.1 [Arabidopsis thaliana]
gi|25403136|pir||E86443 probable G-protein alpha subunit
[imported] - Arabidopsis thaliana
gi|12321289|gb|AAG50710.1|AC079041_3 G-protein, putative
[Arabidopsis thaliana]
gi|12321467|gb|AAG50792.1|AC074309_9 G-protein alpha
subunit, putative [Arabidopsis thaliana]
Length = 848
Score = 224 bits (570), Expect = 1e-57
Identities = 106/145 (73%), Positives = 124/145 (85%), Gaps = 1/145 (0%)
Frame = -3
Query: 647 NKMIXSKELFETMVRHPCFKDTPFVLVLNKYDMFEEKMSRVSLNACEWFSDFCPVLAHQN 468
NKMI SKELFE+MV+HPCFKDTPF+L+LNKYD FEEK++R L +C+WFSDFCPV + N
Sbjct: 704 NKMIQSKELFESMVKHPCFKDTPFILILNKYDQFEEKLNRAPLTSCDWFSDFCPVRTNNN 763
Query: 467 NQSLAHQAYFYVAMKFKDLYASLTGRKLFVAQARARDRVTVDEAFKYIKEVLKWDEEKDE 288
QSLA+QAYFYVAMKFK LY S+TG+KLFV QARARDR VDE FKY++EVLKWDEEK+E
Sbjct: 764 VQSLAYQAYFYVAMKFKLLYFSITGQKLFVWQARARDRANVDEGFKYVREVLKWDEEKEE 823
Query: 287 NYY-GPPEDSFYSTDISSSPYIRQE 216
+Y G EDSFYSTD+SSSPY +E
Sbjct: 824 SYLNGGGEDSFYSTDMSSSPYRPEE 848
>dbj|BAA93022.1| ESTs C74776(E51022),C26123(C116681) correspond to a region of the
predicted gene.~Similar to Arabidopsis thaliana cultivar
Landsberg extra-large G-protein (AF060942) [Oryza sativa
(japonica cultivar-group)]
Length = 867
Score = 212 bits (539), Expect = 4e-54
Identities = 98/144 (68%), Positives = 120/144 (83%)
Frame = -3
Query: 647 NKMIXSKELFETMVRHPCFKDTPFVLVLNKYDMFEEKMSRVSLNACEWFSDFCPVLAHQN 468
NKMI S++LFE +R PCF+D PFVLVLNK+D+FEEK+ RV L+ CEWFSDFCP+ H N
Sbjct: 724 NKMIQSRDLFEATIRQPCFRDMPFVLVLNKFDLFEEKIGRVPLSTCEWFSDFCPLRTHHN 783
Query: 467 NQSLAHQAYFYVAMKFKDLYASLTGRKLFVAQARARDRVTVDEAFKYIKEVLKWDEEKDE 288
NQSLAHQA++YVAMKFK+LYA+ T RKLFV QARARDR+TVDEAFK+I+EVLKW++EKD
Sbjct: 784 NQSLAHQAFYYVAMKFKELYAACTDRKLFVWQARARDRLTVDEAFKFIREVLKWEDEKDG 843
Query: 287 NYYGPPEDSFYSTDISSSPYIRQE 216
Y P E + +T++SSS IRQE
Sbjct: 844 GGYYPDESFYSTTELSSSRLIRQE 867
>gb|AAL34942.1|AC079037_15 Putative G-protein [Oryza sativa]
Length = 583
Score = 170 bits (430), Expect = 2e-41
Identities = 84/142 (59%), Positives = 111/142 (78%), Gaps = 3/142 (2%)
Frame = -3
Query: 647 NKMIXSKELFETMVRHPCFKDTPFVLVLNKYDMFEEKMSRVSLNACEWFSDFCPVLAHQN 468
NKMI S++LFE ++RHP F+DTP VL+LNKYD FEEK+SRV L CEWF+DF PV H
Sbjct: 441 NKMIASRDLFEDVIRHPSFEDTPCVLLLNKYDAFEEKISRVPLTVCEWFADFSPVRPHHT 500
Query: 467 NQ-SLAHQAYFYVAMKFKDLYASLT-GRKLFVAQARARDRVTVDEAFKYIKEVLKWDEEK 294
+Q SLA AY+YVA+KFKDLY+S+ GRKLFV Q +A +R TVD+AF+YI+EVL+WD+ K
Sbjct: 501 SQTSLASHAYYYVAVKFKDLYSSVADGRKLFVFQTKALERRTVDDAFRYIREVLRWDDVK 560
Query: 293 DENY-YGPPEDSFYSTDISSSP 231
+ + Y ++S YS D+++SP
Sbjct: 561 NSDAGYCSADESSYSVDMTTSP 582
>pir||T01135 probable GTP-binding protein (extra large) [imported] - Arabidopsis
thaliana
Length = 901
Score = 128 bits (322), Expect = 6e-29
Identities = 64/142 (45%), Positives = 95/142 (66%), Gaps = 5/142 (3%)
Frame = -3
Query: 647 NKMIXSKELFETMVRHPCFKDTPFVLVLNKYDMFEEKMSRVSLNACEWFSDFCPVL---- 480
NKM+ +K+LFE+++ HP F++ F+L+LNKYD+ EEK+ RV L CEWF DF PV+
Sbjct: 759 NKMLLTKKLFESIITHPIFENMDFLLILNKYDLLEEKVERVPLARCEWFQDFNPVVSRHR 818
Query: 479 -AHQNNQSLAHQAYFYVAMKFKDLYASLTGRKLFVAQARARDRVTVDEAFKYIKEVLKWD 303
++ N +L A+ ++A+KFK Y+SLTG+KLFV+ +++ D +VD + K E+LKW
Sbjct: 819 GSNNGNPTLGQLAFHFMAVKFKRFYSSLTGKKLFVSSSKSLDPNSVDSSLKLAMEILKWS 878
Query: 302 EEKDENYYGPPEDSFYSTDISS 237
EE+ E S YST+ SS
Sbjct: 879 EERTNICMS--EYSMYSTEPSS 898
>ref|NP_565553.1| putative GTP-binding protein (extra large); protein id: At2g23460.1,
supported by cDNA: gi_15451169, supported by cDNA:
gi_3201679 [Arabidopsis thaliana] gi|11357318|pir||T51593
GTP-binding regulatory protein extra-large [validated] -
Arabidopsis thaliana gi|3201680|gb|AAC19352.1|
extra-large G-protein [Arabidopsis thaliana]
gi|20197006|gb|AAC23761.2| putative GTP-binding protein
(extra large) [Arabidopsis thaliana]
Length = 888
Score = 128 bits (322), Expect = 6e-29
Identities = 64/142 (45%), Positives = 95/142 (66%), Gaps = 5/142 (3%)
Frame = -3
Query: 647 NKMIXSKELFETMVRHPCFKDTPFVLVLNKYDMFEEKMSRVSLNACEWFSDFCPVL---- 480
NKM+ +K+LFE+++ HP F++ F+L+LNKYD+ EEK+ RV L CEWF DF PV+
Sbjct: 746 NKMLLTKKLFESIITHPIFENMDFLLILNKYDLLEEKVERVPLARCEWFQDFNPVVSRHR 805
Query: 479 -AHQNNQSLAHQAYFYVAMKFKDLYASLTGRKLFVAQARARDRVTVDEAFKYIKEVLKWD 303
++ N +L A+ ++A+KFK Y+SLTG+KLFV+ +++ D +VD + K E+LKW
Sbjct: 806 GSNNGNPTLGQLAFHFMAVKFKRFYSSLTGKKLFVSSSKSLDPNSVDSSLKLAMEILKWS 865
Query: 302 EEKDENYYGPPEDSFYSTDISS 237
EE+ E S YST+ SS
Sbjct: 866 EERTNICMS--EYSMYSTEPSS 885
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 579,799,615
Number of Sequences: 1393205
Number of extensions: 12945240
Number of successful extensions: 38615
Number of sequences better than 10.0: 343
Number of HSP's better than 10.0 without gapping: 37317
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 38592
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 27576232529
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
