Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000946A_C01 KMC000946A_c01
(602 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_201262.1| glycosyl hydrolase family 3; protein id: At5g64... 120 1e-46
ref|NP_196532.1| beta-glucosidase - like protein; protein id: At... 107 2e-39
gb|AAK38481.1| alpha-L-arabinofuranosidase/beta-D-xylosidase iso... 108 7e-23
gb|AAM00218.1|AF362990_1 beta-D-xylosidase [Prunus persica] 74 2e-22
ref|NP_196535.1| glycosyl hydrolase family 3; protein id: At5g09... 106 2e-22
>ref|NP_201262.1| glycosyl hydrolase family 3; protein id: At5g64570.1 [Arabidopsis
thaliana] gi|10178060|dbj|BAB11424.1| beta-xylosidase
[Arabidopsis thaliana]
Length = 784
Score = 120 bits (301), Expect(2) = 1e-46
Identities = 54/86 (62%), Positives = 69/86 (79%)
Frame = -2
Query: 403 RTRGE*PRDHTVFLFSSPPAVHNAPHKHLVGFEKVHLEGNTEALVKFKVDVCKDLSVVDE 224
R G+ HTVFLF++PPA+H +P KHLVGFEK+ L EA+V+FKV++CKDLSVVDE
Sbjct: 699 RNGGDREGIHTVFLFTTPPAIHGSPRKHLVGFEKIRLGKREEAVVRFKVEICKDLSVVDE 758
Query: 223 LGNRKVALGQHLLHVGDLKHALGVMI 146
+G RK+ LG+HLLHVGDLKH+L + I
Sbjct: 759 IGKRKIGLGKHLLHVGDLKHSLSIRI 784
Score = 88.2 bits (217), Expect(2) = 1e-46
Identities = 41/76 (53%), Positives = 52/76 (67%), Gaps = 6/76 (7%)
Frame = -1
Query: 602 FYKGETVFSFGDGLSYSTIEHKLVKAPQLVSIPLAEDHVCHSLKCKSLDVAGDPCENL-- 429
FY GETV++FGDGLSY+ H LVKAP LVS+ L E+HVC S +C+SLD G CEN
Sbjct: 627 FYTGETVYAFGDGLSYTKFSHTLVKAPSLVSLGLEENHVCRSSECQSLDAIGPHCENAVS 686
Query: 428 ----EFEIHLSIKNQG 393
FE+H+ ++N G
Sbjct: 687 GGGSAFEVHIKVRNGG 702
>ref|NP_196532.1| beta-glucosidase - like protein; protein id: At5g09700.1
[Arabidopsis thaliana] gi|11357195|pir||T49928
beta-glucosidase-like protein - Arabidopsis thaliana
gi|7671419|emb|CAB89360.1| beta-glucosidase-like protein
[Arabidopsis thaliana] gi|9758998|dbj|BAB09525.1|
contains similarity to beta-glucosidase~gene_id:MTH16.12
[Arabidopsis thaliana]
Length = 411
Score = 107 bits (266), Expect(2) = 2e-39
Identities = 49/86 (56%), Positives = 62/86 (71%)
Frame = -2
Query: 403 RTRGE*PRDHTVFLFSSPPAVHNAPHKHLVGFEKVHLEGNTEALVKFKVDVCKDLSVVDE 224
R G+ TVFLF++PP VH +P K L+GFEK+ L E +V+FKVDVCKDL VVDE
Sbjct: 326 RNVGDREGTETVFLFTTPPEVHGSPRKQLLGFEKIRLGKKEETVVRFKVDVCKDLGVVDE 385
Query: 223 LGNRKVALGQHLLHVGDLKHALGVMI 146
+G RK+ALG HLLHVG LKH+ + +
Sbjct: 386 IGKRKLALGHHLLHVGSLKHSFNISV 411
Score = 77.4 bits (189), Expect(2) = 2e-39
Identities = 36/75 (48%), Positives = 49/75 (65%), Gaps = 5/75 (6%)
Frame = -1
Query: 602 FYKGETVFSFGDGLSYSTIEHKLVKAPQLVSIPLAEDHVCHSLKCKSLDVAGDPCENL-- 429
FY GETV++FGDGLSY+ H+L+KAP+ VS+ L E C S +C+SLD G CE
Sbjct: 255 FYIGETVYAFGDGLSYTNFSHQLIKAPKFVSLNLDESQSCRSPECQSLDAIGPHCEKAVG 314
Query: 428 ---EFEIHLSIKNQG 393
+FE+ L ++N G
Sbjct: 315 ERSDFEVQLKVRNVG 329
>gb|AAK38481.1| alpha-L-arabinofuranosidase/beta-D-xylosidase isoenzyme ARA-I
[Hordeum vulgare]
Length = 777
Score = 108 bits (269), Expect = 7e-23
Identities = 54/86 (62%), Positives = 60/86 (68%)
Frame = -2
Query: 403 RTRGE*PRDHTVFLFSSPPAVHNAPHKHLVGFEKVHLEGNTEALVKFKVDVCKDLSVVDE 224
R GE H+V LFSSPP HNAP KHLVGFEKV L V F+VDVC+DLSVVDE
Sbjct: 692 RNAGEVAGAHSVLLFSSPPPAHNAPAKHLVGFEKVSLAPGEAGTVAFRVDVCRDLSVVDE 751
Query: 223 LGNRKVALGQHLLHVGDLKHALGVMI 146
LG RKVALG H LH GDLKH + + +
Sbjct: 752 LGGRKVALGGHTLHDGDLKHTVELRV 777
Score = 85.5 bits (210), Expect = 5e-16
Identities = 43/86 (50%), Positives = 61/86 (70%), Gaps = 1/86 (1%)
Frame = -1
Query: 602 FYKGETVFSFGDGLSYSTIEHKLVKAP-QLVSIPLAEDHVCHSLKCKSLDVAGDPCENLE 426
FY G+TVF+FGDGLSY+ + H LV AP VS+ LAEDH+C + +C S++ AGD C++L
Sbjct: 625 FYTGDTVFAFGDGLSYTKMSHSLVSAPPSYVSMRLAEDHLCRAEECASVEAAGDHCDDLA 684
Query: 425 FEIHLSIKNQGRMTT*SHCVLVLFSS 348
++ L ++N G + +H VL LFSS
Sbjct: 685 LDVKLQVRNAGEVAG-AHSVL-LFSS 708
>gb|AAM00218.1|AF362990_1 beta-D-xylosidase [Prunus persica]
Length = 461
Score = 73.9 bits (180), Expect(2) = 2e-22
Identities = 34/71 (47%), Positives = 46/71 (63%)
Frame = -2
Query: 376 HTVFLFSSPPAVHNAPHKHLVGFEKVHLEGNTEALVKFKVDVCKDLSVVDELGNRKVALG 197
HT+ +F+SPP A K L+GF K+H+ +E V+ V VCK LSVVD G R++ LG
Sbjct: 378 HTLLVFTSPPDGKWASSKQLMGFHKIHIATGSEKRVRIAVHVCKHLSVVDRFGIRRIPLG 437
Query: 196 QHLLHVGDLKH 164
+H L +GDL H
Sbjct: 438 EHKLQIGDLSH 448
Score = 53.5 bits (127), Expect(2) = 2e-22
Identities = 33/87 (37%), Positives = 47/87 (53%), Gaps = 3/87 (3%)
Frame = -1
Query: 602 FYKGETVFSFGDGLSYSTIEHKLVKAPQLVSIPLA--EDHVCHSLKCKSLDVAGDPCENL 429
FY G VF FG GLSY+T H L P LVS+PL + ++ K++ V+ C L
Sbjct: 300 FYIGPVVFPFGLGLSYTTFAHNLAHGPTLVSVPLTSLKATANSTMLSKTVRVSHPDCNAL 359
Query: 428 E-FEIHLSIKNQGRMTT*SHCVLVLFS 351
++H+ +KN G M +H +LV S
Sbjct: 360 SPLDVHVDVKNTGSMDG-THTLLVFTS 385
>ref|NP_196535.1| glycosyl hydrolase family 3; protein id: At5g09730.1, supported by
cDNA: gi_15450734 [Arabidopsis thaliana]
gi|11357197|pir||T49925 beta-xylosidase-like protein -
Arabidopsis thaliana gi|7671416|emb|CAB89357.1|
beta-xylosidase-like protein [Arabidopsis thaliana]
gi|9759004|dbj|BAB09531.1| beta-xylosidase [Arabidopsis
thaliana] gi|15450735|gb|AAK96639.1| AT5g09730/F17I14_80
[Arabidopsis thaliana]
Length = 773
Score = 106 bits (265), Expect = 2e-22
Identities = 49/86 (56%), Positives = 64/86 (73%)
Frame = -2
Query: 403 RTRGE*PRDHTVFLFSSPPAVHNAPHKHLVGFEKVHLEGNTEALVKFKVDVCKDLSVVDE 224
+ G+ HTVFLF++ P VH +P K L+GFEK+ L + EA+V+F V+VCKDLSVVDE
Sbjct: 688 KNTGDRAGSHTVFLFTTSPQVHGSPIKQLLGFEKIRLGKSEEAVVRFNVNVCKDLSVVDE 747
Query: 223 LGNRKVALGQHLLHVGDLKHALGVMI 146
G RK+ALG HLLHVG LKH+L + +
Sbjct: 748 TGKRKIALGHHLLHVGSLKHSLNISV 773
Score = 86.7 bits (213), Expect = 2e-16
Identities = 42/90 (46%), Positives = 60/90 (66%), Gaps = 5/90 (5%)
Frame = -1
Query: 602 FYKGETVFSFGDGLSYSTIEHKLVKAPQLVSIPLAEDHVCHSLKCKSLDVAGDPCENL-- 429
FY GETV++F D L+Y+ +H+L+KAP+LVS+ L E+H C S +C+SLD G CEN
Sbjct: 617 FYTGETVYAFADALTYTKFDHQLIKAPRLVSLSLDENHPCRSSECQSLDAIGPHCENAVE 676
Query: 428 ---EFEIHLSIKNQGRMTT*SHCVLVLFSS 348
+FE+HL++KN G SH V + +S
Sbjct: 677 GGSDFEVHLNVKNTGDRAG-SHTVFLFTTS 705
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 502,350,558
Number of Sequences: 1393205
Number of extensions: 10412328
Number of successful extensions: 20434
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 19771
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 20423
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 23711793746
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
