Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000649A_C03 KMC000649A_c03
(842 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_201189.1| glycosyl hydrolase family 31; protein id: At5g6... 153 3e-36
pir||T07391 probable alpha-glucosidase (EC 3.2.1.20) - potato gi... 129 4e-29
gb|AAB18921.1| ModA [Dictyostelium discoideum] 76 7e-13
gb|EAA12063.1| agCP9072 [Anopheles gambiae str. PEST] 69 7e-11
ref|XP_215144.1| similar to alpha glucosidase II alpha subunit; ... 63 5e-09
>ref|NP_201189.1| glycosyl hydrolase family 31; protein id: At5g63840.1 [Arabidopsis
thaliana] gi|10177672|dbj|BAB11032.1| glucosidase II
alpha subunit [Arabidopsis thaliana]
Length = 921
Score = 153 bits (387), Expect = 3e-36
Identities = 79/131 (60%), Positives = 96/131 (72%), Gaps = 3/131 (2%)
Frame = -1
Query: 842 QMTNDPYTLVIALNSSQAAEGEIYIDDGKSFDFLQGGYIHRRFIFSNGKLSSVDLAPSSG 663
QM NDPYTLV+ALNSSQ AEGE+YIDDGKSF+F +G YIHRRF+FS G L+S +LAP
Sbjct: 791 QMDNDPYTLVVALNSSQEAEGELYIDDGKSFEFRRGSYIHRRFVFSKGVLTSTNLAPP-- 848
Query: 662 GNVRYSSDVVIERIILLGHAPGSKNALIEPSNQKVNIELGPL---WVHRAQSPAVTTIRK 492
R SS +I+RIILLGH+ G K+AL+EP NQK IE+GPL + + V TIRK
Sbjct: 849 -EARLSSQCLIDRIILLGHSSGPKSALVEPLNQKAEIEMGPLRMGGLVASSGTKVLTIRK 907
Query: 491 PNVPVAEDWTI 459
P V V +DWT+
Sbjct: 908 PGVRVDQDWTV 918
>pir||T07391 probable alpha-glucosidase (EC 3.2.1.20) - potato
gi|2648032|emb|CAA04707.1| alpha-glucosidase [Solanum
tuberosum]
Length = 919
Score = 129 bits (325), Expect = 4e-29
Identities = 69/128 (53%), Positives = 91/128 (70%)
Frame = -1
Query: 842 QMTNDPYTLVIALNSSQAAEGEIYIDDGKSFDFLQGGYIHRRFIFSNGKLSSVDLAPSSG 663
QM NDPYTLVIALNSS+AAEGE+YIDDGKS++F QG +I + + + LA +
Sbjct: 795 QMENDPYTLVIALNSSKAAEGELYIDDGKSYEFKQGAFILKWEAYIFQMQPRLQLAVT-- 852
Query: 662 GNVRYSSDVVIERIILLGHAPGSKNALIEPSNQKVNIELGPLWVHRAQSPAVTTIRKPNV 483
+ S+ +ERIILLG +PG+K ALIEP N+KV IELGPL++ + +V TIRKPNV
Sbjct: 853 ---HFPSECTVERIILLGLSPGAKTALIEPGNKKVEIELGPLFI-QGNRGSVPTIRKPNV 908
Query: 482 PVAEDWTI 459
+ +DW+I
Sbjct: 909 RITDDWSI 916
>gb|AAB18921.1| ModA [Dictyostelium discoideum]
Length = 943
Score = 75.9 bits (185), Expect = 7e-13
Identities = 45/128 (35%), Positives = 74/128 (57%)
Frame = -1
Query: 842 QMTNDPYTLVIALNSSQAAEGEIYIDDGKSFDFLQGGYIHRRFIFSNGKLSSVDLAPSSG 663
QM +DPYT+ IAL+SS++A+G++YIDD SFD+ +G +++R+F F + LS D S+
Sbjct: 822 QMRDDPYTIRIALDSSKSAQGQLYIDDEHSFDYKKGKFLYRQFTFKDNVLSFSD--ASNK 879
Query: 662 GNVRYSSDVVIERIILLGHAPGSKNALIEPSNQKVNIELGPLWVHRAQSPAVTTIRKPNV 483
+ Y +V IE+I++LG +K++ E + + TIRKP++
Sbjct: 880 SSTSYKPNVTIEKIVILGVQKPHSITCNITGKEKLSFEYD-------STLSKLTIRKPDL 932
Query: 482 PVAEDWTI 459
V D+ I
Sbjct: 933 LVDTDFII 940
>gb|EAA12063.1| agCP9072 [Anopheles gambiae str. PEST]
Length = 1023
Score = 69.3 bits (168), Expect = 7e-11
Identities = 42/131 (32%), Positives = 69/131 (52%), Gaps = 4/131 (3%)
Frame = -1
Query: 839 MTNDPYTLVIALNSSQAAEGEIYIDDGKSFDFLQGGYIHRRFIFSNGKLSS--VDLAPSS 666
M +DPYTL +AL+ A+G +YIDD +S+++ G Y++ F F +G LSS +D+ S
Sbjct: 901 MRHDPYTLTVALDREGRAKGTLYIDDEESYEYRHGHYLYLEFEFRDGVLSSRKIDMTAS- 959
Query: 665 GGNVRYSSDVVIERIIL--LGHAPGSKNALIEPSNQKVNIELGPLWVHRAQSPAVTTIRK 492
Y +D +ER+++ LG P + + S +H + V T+RK
Sbjct: 960 -----YKTDTWLERVVIAGLGKQPTAATLYTDASGAASG------RLHLERDGDVVTVRK 1008
Query: 491 PNVPVAEDWTI 459
P V + + W+I
Sbjct: 1009 PGVSMRDAWSI 1019
>ref|XP_215144.1| similar to alpha glucosidase II alpha subunit; likely ortholog of
mouse G2an alpha glucosidase 2, alpha neutral subunit
[Homo sapiens] [Rattus norvegicus]
Length = 219
Score = 63.2 bits (152), Expect = 5e-09
Identities = 42/128 (32%), Positives = 69/128 (53%), Gaps = 1/128 (0%)
Frame = -1
Query: 839 MTNDPYTLVIALNSSQAAEGEIYIDDGKSFDFL-QGGYIHRRFIFSNGKLSSVDLAPSSG 663
M DP TL +AL++ A+GE+++DDG +F++ + ++ RRF FS L S P
Sbjct: 98 MKEDPITLFVALSAQGTAQGELFLDDGHTFNYQNRHEFLLRRFSFSGNTLVSSSADPKG- 156
Query: 662 GNVRYSSDVVIERIILLGHAPGSKNALIEPSNQKVNIELGPLWVHRAQSPAVTTIRKPNV 483
+ V IER++++G G A++ + L + H ++ +V T+RKP V
Sbjct: 157 ---HLETPVWIERVVIMG--AGKPAAVVLQTKGSPESRLS--FQHDPET-SVLTLRKPGV 208
Query: 482 PVAEDWTI 459
VA DW+I
Sbjct: 209 NVASDWSI 216
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 728,919,199
Number of Sequences: 1393205
Number of extensions: 16114793
Number of successful extensions: 37090
Number of sequences better than 10.0: 71
Number of HSP's better than 10.0 without gapping: 35796
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 37058
length of database: 448,689,247
effective HSP length: 122
effective length of database: 278,718,237
effective search space used: 44037481446
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
