Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000518A_C01 KMC000518A_c01
(666 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_191645.1| guanine nucleotide exchange factor - like prote... 146 2e-34
gb|AAM00191.1|AF262215_1 guanine nucleotide-exchange protein GEP... 139 4e-32
ref|NP_171698.1| hypothetical protein; protein id: At1g01960.1 [... 124 9e-28
dbj|BAA82387.1| Similar to SEC7 protein, Saccharomyces cerevisia... 89 4e-17
pir||T05647 hypothetical protein F20D10.320 - Arabidopsis thalia... 76 5e-13
>ref|NP_191645.1| guanine nucleotide exchange factor - like protein; protein id:
At3g60860.1 [Arabidopsis thaliana]
gi|11357320|pir||T47897 guanine nucleotide exchange
factor-like protein - Arabidopsis thaliana
gi|7329696|emb|CAB82690.1| guanine nucleotide exchange
factor-like protein [Arabidopsis thaliana]
Length = 1793
Score = 146 bits (369), Expect = 2e-34
Identities = 75/114 (65%), Positives = 89/114 (77%), Gaps = 2/114 (1%)
Frame = -3
Query: 664 SHLVRFCQEVLEFYIEVAGFGQK--SESSNGRKPHWSIPLGSGKRRELAARSPLVVATLQ 491
S LV CQEVL FYIE + +K SESS + W IPLGSGKRREL+AR+PL+VATLQ
Sbjct: 1680 SLLVNICQEVLNFYIETSSSAKKLQSESSRASEYRWRIPLGSGKRRELSARAPLIVATLQ 1739
Query: 490 AICSLGDISFEKNLSHFFPLLSSLVSCEHGSNDVQVALSDMLSLSVGSVLLQSC 329
A+C+L + SFEKNL FPLL++L+SCEHGSN+VQ AL+DML LSVG VLLQ C
Sbjct: 1740 AMCTLDEASFEKNLKCLFPLLANLISCEHGSNEVQTALADMLGLSVGPVLLQWC 1793
>gb|AAM00191.1|AF262215_1 guanine nucleotide-exchange protein GEP2 [Oryza sativa]
Length = 1789
Score = 139 bits (349), Expect = 4e-32
Identities = 73/114 (64%), Positives = 86/114 (75%), Gaps = 2/114 (1%)
Frame = -3
Query: 664 SHLVRFCQEVLEFYIEVAGFGQKSESSNGRKP--HWSIPLGSGKRRELAARSPLVVATLQ 491
SHLV C+EVLE Y+ A S+ S +P HW IP+GS KRRELAAR+PLVV+TLQ
Sbjct: 1679 SHLVGLCKEVLEVYLSTAN---PSQLSGAPQPLGHWLIPVGSSKRRELAARAPLVVSTLQ 1735
Query: 490 AICSLGDISFEKNLSHFFPLLSSLVSCEHGSNDVQVALSDMLSLSVGSVLLQSC 329
AI LGD SFEKNL FFPLL+ L+SCEHGS++VQVALSDM S VG V+LQ+C
Sbjct: 1736 AISGLGDSSFEKNLGQFFPLLAGLISCEHGSSEVQVALSDMFSTWVGPVVLQTC 1789
>ref|NP_171698.1| hypothetical protein; protein id: At1g01960.1 [Arabidopsis thaliana]
gi|25518540|pir||E86151 hypothetical protein F22M8.9 -
Arabidopsis thaliana gi|8570447|gb|AAF76474.1|AC020622_8
Contains similarity to a guanine nucleotide exchange
factor from Homo sapiens gb|AF111162 and contains a Sec7
PF|01369 domain. [Arabidopsis thaliana]
Length = 1750
Score = 124 bits (312), Expect = 9e-28
Identities = 61/112 (54%), Positives = 84/112 (74%)
Frame = -3
Query: 664 SHLVRFCQEVLEFYIEVAGFGQKSESSNGRKPHWSIPLGSGKRRELAARSPLVVATLQAI 485
SHL+ C+EVLEFYI ++ K +SS W++P GSGK++EL AR+PLVVA +Q +
Sbjct: 1645 SHLISLCREVLEFYINISC--SKEQSSR-----WAVPSGSGKKKELTARAPLVVAAIQTL 1697
Query: 484 CSLGDISFEKNLSHFFPLLSSLVSCEHGSNDVQVALSDMLSLSVGSVLLQSC 329
++G+ F+KNL FPL+++L+SCEHGS +VQVALSDML S+G VLL+SC
Sbjct: 1698 GNMGESLFKKNLPELFPLIATLISCEHGSGEVQVALSDMLQTSMGPVLLRSC 1749
>dbj|BAA82387.1| Similar to SEC7 protein, Saccharomyces cerevisiae, PIR2:S49764;
Contains Immunoglobulins and major histocompatibility
complex proteins signature. (AL022604) [Oryza sativa
(japonica cultivar-group)]
Length = 1687
Score = 89.4 bits (220), Expect = 4e-17
Identities = 44/110 (40%), Positives = 72/110 (65%), Gaps = 2/110 (1%)
Frame = -3
Query: 664 SHLVRFCQEVLEFYIEVAGFGQKSESSNGRKP--HWSIPLGSGKRRELAARSPLVVATLQ 491
SH++ +++L Y++ AG ++S S+GR P W +PLG+ K+ EL+AR+ LV+ +Q
Sbjct: 1577 SHIMLVSEKILRKYLKCAG-RERSNDSSGRDPALRWKLPLGTAKKEELSARTSLVLHVMQ 1635
Query: 490 AICSLGDISFEKNLSHFFPLLSSLVSCEHGSNDVQVALSDMLSLSVGSVL 341
+ L F +NL FFPLL++L+ CEH S +VQ+AL D+ S+G ++
Sbjct: 1636 LLGGLERDCFRRNLPLFFPLLTNLIRCEHSSGEVQLALYDIFQSSIGPII 1685
>pir||T05647 hypothetical protein F20D10.320 - Arabidopsis thaliana
gi|4467126|emb|CAB37560.1| guanine nucleotide-exchange
protein-like [Arabidopsis thaliana]
gi|7270804|emb|CAB80485.1| guanine nucleotide-exchange
protein-like [Arabidopsis thaliana]
Length = 1643
Score = 75.9 bits (185), Expect = 5e-13
Identities = 42/109 (38%), Positives = 65/109 (59%), Gaps = 1/109 (0%)
Frame = -3
Query: 664 SHLVRFCQEVLEFYIEVAGFGQKSESSNGRKP-HWSIPLGSGKRRELAARSPLVVATLQA 488
S L+ C ++L+ Y++ F Q E R+P +W +P+G+ + E AARSPLVVA L+A
Sbjct: 1534 SQLMTVCMQILKMYLKCTLF-QGDELEETRQPKNWILPMGAASKEEAAARSPLVVAVLKA 1592
Query: 487 ICSLGDISFEKNLSHFFPLLSSLVSCEHGSNDVQVALSDMLSLSVGSVL 341
+ L SF++ +FFPLL LV EH S+ V LS + +G+++
Sbjct: 1593 LRELKRDSFKRYAPNFFPLLVELVRSEHSSSQVPQVLSTVFHTCMGAMM 1641
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 528,019,181
Number of Sequences: 1393205
Number of extensions: 10750063
Number of successful extensions: 25183
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 24685
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25179
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 28855580904
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
