Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000227A_C02 KMC000227A_c02
(603 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_029947.2| E2, ubiquitin-conjugating enzyme, putative; pro... 132 4e-30
ref|NP_179284.1| putative ubiquitin-conjugating enzyme; protein ... 109 2e-23
dbj|BAB21187.1| contains ESTs D22419(C10991),AU100646(C10991)~si... 103 2e-21
dbj|BAB90019.1| putative ubiquitin-conjugating protein [Oryza sa... 90 3e-17
dbj|BAA96594.1| unnamed protein product [Oryza sativa (japonica ... 81 1e-14
>ref|NP_029947.2| E2, ubiquitin-conjugating enzyme, putative; protein id: At2g33770.1,
supported by cDNA: gi_18377677, supported by cDNA:
gi_20259098 [Arabidopsis thaliana]
gi|18377678|gb|AAL66989.1| putative E2,
ubiquitin-conjugating enzyme [Arabidopsis thaliana]
gi|20259099|gb|AAM14265.1| putative ubiquitin-conjugating
enzyme E2 [Arabidopsis thaliana]
Length = 907
Score = 132 bits (331), Expect = 4e-30
Identities = 62/101 (61%), Positives = 78/101 (76%)
Frame = -2
Query: 602 EGEKDSVSYNENAFLLTTKSMLYILRKPPKHFEALVEEHFRERSQRILHACQAYLEGASL 423
EGEK+SVSYNENAFL+T KSM+ +LRKPPKHFE LV++HF R+Q +L AC+AY+EG +
Sbjct: 801 EGEKNSVSYNENAFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPV 860
Query: 422 GCGKSEYENQKGTSTGFKIMLAKLFPNLVEAFSSKGIDCSQ 300
G + N STGFKIML+KL+P L+EAFS G+DC Q
Sbjct: 861 GSSANLQGNSTTNSTGFKIMLSKLYPKLLEAFSEIGVDCVQ 901
>ref|NP_179284.1| putative ubiquitin-conjugating enzyme; protein id: At2g16920.1
[Arabidopsis thaliana] gi|25411753|pir||H84545 probable
ubiquitin-conjugating enzyme [imported] - Arabidopsis
thaliana gi|3757521|gb|AAC64223.1| putative
ubiquitin-conjugating enzyme [Arabidopsis thaliana]
Length = 1102
Score = 109 bits (273), Expect = 2e-23
Identities = 53/108 (49%), Positives = 71/108 (65%), Gaps = 6/108 (5%)
Frame = -2
Query: 602 EGEKDSVSYNENAFLLTTKSMLYILRKPPKHFEALVEEHFRERSQRILHACQAYLEGASL 423
EGEK+S+ YNEN FLL K+M+Y++RKPPK FE L+++HFR+R IL AC AY++G +
Sbjct: 989 EGEKNSLGYNENTFLLNCKTMMYLMRKPPKDFEELIKDHFRKRGYYILKACDAYMKGYLI 1048
Query: 422 GCGK------SEYENQKGTSTGFKIMLAKLFPNLVEAFSSKGIDCSQF 297
G E + TS GFK+MLAK+ P L A S G DC++F
Sbjct: 1049 GSLTKDASVIDERSSANSTSVGFKLMLAKIAPKLFSALSEVGADCNEF 1096
>dbj|BAB21187.1| contains ESTs D22419(C10991),AU100646(C10991)~similar to Arabidopsis
thaliana chromosome 2, At2g16920~unknown protein [Oryza
sativa (japonica cultivar-group)]
gi|14090383|dbj|BAB55541.1| P0037C04.30 [Oryza sativa
(japonica cultivar-group)]
Length = 1067
Score = 103 bits (257), Expect = 2e-21
Identities = 49/107 (45%), Positives = 69/107 (63%), Gaps = 5/107 (4%)
Frame = -2
Query: 602 EGEKDSVSYNENAFLLTTKSMLYILRKPPKHFEALVEEHFRERSQRILHACQAYLEGASL 423
EGEK+++ YNEN +LL+ KSMLYILR+PP HFE + HF +R + IL AC+AYL+G +
Sbjct: 949 EGEKNALPYNENTYLLSLKSMLYILRRPPMHFEDFAKSHFSKRGKYILKACEAYLQGNGV 1008
Query: 422 G-----CGKSEYENQKGTSTGFKIMLAKLFPNLVEAFSSKGIDCSQF 297
G +E ++ S GFK+ LAK+ P L+ A G +C Q+
Sbjct: 1009 GTLTDDACTTERSKEQPCSVGFKLALAKIMPRLITALKDAGANCDQY 1055
>dbj|BAB90019.1| putative ubiquitin-conjugating protein [Oryza sativa (japonica
cultivar-group)]
Length = 493
Score = 89.7 bits (221), Expect = 3e-17
Identities = 46/105 (43%), Positives = 63/105 (59%), Gaps = 4/105 (3%)
Frame = -2
Query: 596 EKDSVSYNENAFLLTTKSMLYILRKPPKHFEALVEEHFRERSQRILHACQAYLEGASLGC 417
EK S++YN+ FLL+ K+MLY LR PPKHF+ + HF + IL C AY++GA +G
Sbjct: 382 EKKSIAYNQETFLLSCKTMLYSLRNPPKHFDDFIIGHFHKYGHSILIGCNAYMDGAQVGS 441
Query: 416 ----GKSEYENQKGTSTGFKIMLAKLFPNLVEAFSSKGIDCSQFV 294
K+ + KG ST FK L KLF L+ F G+DC +F+
Sbjct: 442 IIGGVKAIDKGNKGCSTKFKGSLKKLFEELMMEFIGIGVDCHEFM 486
>dbj|BAA96594.1| unnamed protein product [Oryza sativa (japonica cultivar-group)]
Length = 359
Score = 80.9 bits (198), Expect = 1e-14
Identities = 36/65 (55%), Positives = 49/65 (75%)
Frame = -2
Query: 599 GEKDSVSYNENAFLLTTKSMLYILRKPPKHFEALVEEHFRERSQRILHACQAYLEGASLG 420
GEK S++YN+ AFLL+ ++M+Y LRKPPKHFE+LV HF ER + IL AC AY+ GA +G
Sbjct: 238 GEKYSLAYNQTAFLLSCRTMMYSLRKPPKHFESLVARHFHERERVILDACDAYISGAVVG 297
Query: 419 CGKSE 405
++
Sbjct: 298 SSSAK 302
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 505,737,960
Number of Sequences: 1393205
Number of extensions: 10276029
Number of successful extensions: 24087
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 23580
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24082
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 23711793746
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
