Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000086A_C03 KMC000086A_c03
(703 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||T04242 abdominal segment formation protein pumilio homolog ... 99 4e-20
ref|NP_567733.1| pumilio-like protein; protein id: At4g25880.1, ... 99 4e-20
ref|NP_188660.2| pumilio-family RNA-binding protein, putative; p... 99 7e-20
ref|NP_180478.1| pumilio-family RNA-binding protein, putative; p... 91 2e-17
ref|NP_180483.1| pumilio-family RNA-binding protein, putative; p... 91 2e-17
>pir||T04242 abdominal segment formation protein pumilio homolog F14M19.160 -
Arabidopsis thaliana gi|4539306|emb|CAB39609.1|
pumilio-like protein [Arabidopsis thaliana]
gi|7269439|emb|CAB79443.1| pumilio-like protein
[Arabidopsis thaliana]
Length = 849
Score = 99.4 bits (246), Expect = 4e-20
Identities = 48/76 (63%), Positives = 59/76 (77%), Gaps = 1/76 (1%)
Frame = -3
Query: 701 LIAEIIGQDEQNNNLLTMMKDQFANYVVQKVIDLCSENQRATLLSHIRVHAQALKKYTYG 522
+I EI G DE N+LL MMKDQ+ NYVVQK+ + C+ +QR TL S +R+HA ALKKYTYG
Sbjct: 770 IIKEIAGPDESYNSLLMMMKDQYGNYVVQKIFETCTADQRLTLFSRVRMHASALKKYTYG 829
Query: 521 KHIVARLEQ-QFEEDQ 477
KHIV+RLEQ EE+Q
Sbjct: 830 KHIVSRLEQPSIEENQ 845
Score = 33.5 bits (75), Expect = 3.0
Identities = 17/58 (29%), Positives = 25/58 (42%)
Frame = -3
Query: 650 MMKDQFANYVVQKVIDLCSENQRATLLSHIRVHAQALKKYTYGKHIVARLEQQFEEDQ 477
+M D F NYV+QK + + QR L + L YG ++ + E DQ
Sbjct: 564 LMTDVFGNYVIQKFFEYGNSTQRKELADQLMGQIVPLSLQMYGCRVIQKALDVIEPDQ 621
Score = 33.1 bits (74), Expect = 3.9
Identities = 16/65 (24%), Positives = 33/65 (50%)
Frame = -3
Query: 674 EQNNNLLTMMKDQFANYVVQKVIDLCSENQRATLLSHIRVHAQALKKYTYGKHIVARLEQ 495
E + ++ ++DQ N+V+QK I+ ++ +L R +L + YG ++ RL +
Sbjct: 628 ELDGQVMRCVRDQNGNHVIQKCIENIPADKVGFMLYAFRGQVSSLSMHPYGCRVIQRLLE 687
Query: 494 QFEED 480
+ D
Sbjct: 688 RCSHD 692
>ref|NP_567733.1| pumilio-like protein; protein id: At4g25880.1, supported by cDNA:
gi_13430771 [Arabidopsis thaliana]
gi|13430772|gb|AAK26008.1|AF360298_1 putative pumilio
protein [Arabidopsis thaliana] gi|22136912|gb|AAM91800.1|
putative pumilio protein [Arabidopsis thaliana]
Length = 861
Score = 99.4 bits (246), Expect = 4e-20
Identities = 48/76 (63%), Positives = 59/76 (77%), Gaps = 1/76 (1%)
Frame = -3
Query: 701 LIAEIIGQDEQNNNLLTMMKDQFANYVVQKVIDLCSENQRATLLSHIRVHAQALKKYTYG 522
+I EI G DE N+LL MMKDQ+ NYVVQK+ + C+ +QR TL S +R+HA ALKKYTYG
Sbjct: 782 IIKEIAGPDESYNSLLMMMKDQYGNYVVQKIFETCTADQRLTLFSRVRMHASALKKYTYG 841
Query: 521 KHIVARLEQ-QFEEDQ 477
KHIV+RLEQ EE+Q
Sbjct: 842 KHIVSRLEQPSIEENQ 857
Score = 33.5 bits (75), Expect = 3.0
Identities = 17/58 (29%), Positives = 25/58 (42%)
Frame = -3
Query: 650 MMKDQFANYVVQKVIDLCSENQRATLLSHIRVHAQALKKYTYGKHIVARLEQQFEEDQ 477
+M D F NYV+QK + + QR L + L YG ++ + E DQ
Sbjct: 576 LMTDVFGNYVIQKFFEYGNSTQRKELADQLMGQIVPLSLQMYGCRVIQKALDVIEPDQ 633
Score = 33.1 bits (74), Expect = 3.9
Identities = 16/65 (24%), Positives = 33/65 (50%)
Frame = -3
Query: 674 EQNNNLLTMMKDQFANYVVQKVIDLCSENQRATLLSHIRVHAQALKKYTYGKHIVARLEQ 495
E + ++ ++DQ N+V+QK I+ ++ +L R +L + YG ++ RL +
Sbjct: 640 ELDGQVMRCVRDQNGNHVIQKCIENIPADKVGFMLYAFRGQVSSLSMHPYGCRVIQRLLE 699
Query: 494 QFEED 480
+ D
Sbjct: 700 RCSHD 704
>ref|NP_188660.2| pumilio-family RNA-binding protein, putative; protein id: At3g20250.1
[Arabidopsis thaliana] gi|9293981|dbj|BAB01884.1| RNA
binding protein-like [Arabidopsis thaliana]
Length = 962
Score = 98.6 bits (244), Expect = 7e-20
Identities = 45/78 (57%), Positives = 61/78 (77%)
Frame = -3
Query: 701 LIAEIIGQDEQNNNLLTMMKDQFANYVVQKVIDLCSENQRATLLSHIRVHAQALKKYTYG 522
LI EI+G+ E++N+LL MMKDQFANYVVQKV+++ + QR L+ +++H Q+L+KYTYG
Sbjct: 879 LIEEIMGKSEEDNHLLAMMKDQFANYVVQKVLEISKDQQREILVQRMKIHLQSLRKYTYG 938
Query: 521 KHIVARLEQQFEEDQSPS 468
KHIVAR EQ F E+ S
Sbjct: 939 KHIVARFEQLFGEESEVS 956
Score = 35.8 bits (81), Expect = 0.60
Identities = 16/66 (24%), Positives = 34/66 (51%)
Frame = -3
Query: 674 EQNNNLLTMMKDQFANYVVQKVIDLCSENQRATLLSHIRVHAQALKKYTYGKHIVARLEQ 495
E + N+L ++DQ N+V+QK I+ + +++ R L + YG ++ R+ +
Sbjct: 737 ELDGNVLKCVRDQNGNHVIQKCIESMPAGRIGFVIAAFRGQVATLSTHPYGCRVIQRILE 796
Query: 494 QFEEDQ 477
+D+
Sbjct: 797 HCSDDE 802
Score = 33.9 bits (76), Expect = 2.3
Identities = 17/58 (29%), Positives = 29/58 (49%)
Frame = -3
Query: 650 MMKDQFANYVVQKVIDLCSENQRATLLSHIRVHAQALKKYTYGKHIVARLEQQFEEDQ 477
+M D F NYV+QK I+ + QR L+ + +L YG ++ + + + DQ
Sbjct: 673 LMTDVFGNYVIQKFIEHGTPAQREELVKQLAGQMVSLSLQMYGCRVIQKALEVIDVDQ 730
>ref|NP_180478.1| pumilio-family RNA-binding protein, putative; protein id: At2g29140.1
[Arabidopsis thaliana] gi|25354254|pir||A84693
hypothetical protein At2g29140 [imported] - Arabidopsis
thaliana gi|3980417|gb|AAC95220.1| putative pumilio/Mpt5
family RNA-binding protein [Arabidopsis thaliana]
Length = 964
Score = 90.9 bits (224), Expect = 2e-17
Identities = 41/69 (59%), Positives = 54/69 (77%)
Frame = -3
Query: 701 LIAEIIGQDEQNNNLLTMMKDQFANYVVQKVIDLCSENQRATLLSHIRVHAQALKKYTYG 522
L+ E++G ++N L MMKDQFANYVVQKV++ C + QR +L+ I+VH ALKKYTYG
Sbjct: 876 LVNEMLGTTDENEPLQAMMKDQFANYVVQKVLETCDDQQRELILTRIKVHLNALKKYTYG 935
Query: 521 KHIVARLEQ 495
KHIVAR+E+
Sbjct: 936 KHIVARVEK 944
Score = 33.5 bits (75), Expect = 3.0
Identities = 16/58 (27%), Positives = 30/58 (51%)
Frame = -3
Query: 674 EQNNNLLTMMKDQFANYVVQKVIDLCSENQRATLLSHIRVHAQALKKYTYGKHIVARL 501
E + +++ ++DQ N+VVQK I+ E ++S H L + YG ++ R+
Sbjct: 734 ELDGHVMRCVRDQNGNHVVQKCIECVPEENIEFIISTFFGHVVTLSTHPYGCRVIQRV 791
>ref|NP_180483.1| pumilio-family RNA-binding protein, putative; protein id: At2g29200.1
[Arabidopsis thaliana] gi|25354258|pir||F84693
hypothetical protein At2g29200 [imported] - Arabidopsis
thaliana gi|3980412|gb|AAC95215.1| putative pumilio/Mpt5
family RNA-binding protein [Arabidopsis thaliana]
Length = 968
Score = 90.5 bits (223), Expect = 2e-17
Identities = 40/69 (57%), Positives = 54/69 (77%)
Frame = -3
Query: 701 LIAEIIGQDEQNNNLLTMMKDQFANYVVQKVIDLCSENQRATLLSHIRVHAQALKKYTYG 522
L+ E++G ++N L MMKDQFANYVVQKV++ C + QR +L+ I+VH ALKKYTYG
Sbjct: 880 LVNEMLGTTDENEPLQAMMKDQFANYVVQKVLETCDDQQRELILTRIKVHLTALKKYTYG 939
Query: 521 KHIVARLEQ 495
KH+VAR+E+
Sbjct: 940 KHVVARIEK 948
Score = 34.3 bits (77), Expect = 1.7
Identities = 17/69 (24%), Positives = 34/69 (48%)
Frame = -3
Query: 674 EQNNNLLTMMKDQFANYVVQKVIDLCSENQRATLLSHIRVHAQALKKYTYGKHIVARLEQ 495
E + +++ ++DQ N+VVQK I+ E ++S H L + YG ++ R+ +
Sbjct: 738 ELDGHVMRCVRDQNGNHVVQKCIECVPEENIEFIISTFFGHVVTLSTHPYGCRVIQRVLE 797
Query: 494 QFEEDQSPS 468
+ + S
Sbjct: 798 HCHDPDTQS 806
Score = 32.7 bits (73), Expect = 5.0
Identities = 18/60 (30%), Positives = 27/60 (45%)
Frame = -3
Query: 656 LTMMKDQFANYVVQKVIDLCSENQRATLLSHIRVHAQALKKYTYGKHIVARLEQQFEEDQ 477
L +M D F NYV+QK + QR L + H L YG ++ + + + DQ
Sbjct: 672 LVLMTDVFGNYVIQKFFEHGLPPQRRELAEKLFDHVLPLSLQMYGCRVIQKAIEVVDLDQ 731
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 571,254,864
Number of Sequences: 1393205
Number of extensions: 11759799
Number of successful extensions: 23049
Number of sequences better than 10.0: 86
Number of HSP's better than 10.0 without gapping: 22141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23045
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 32250355128
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
