Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000057A_C02 KMC000057A_c02
(961 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAO13795.1|AF352575_1 putative sterol 4-alpha-methyl-oxidase ... 449 e-125
ref|NP_563789.1| expressed protein; protein id: At1g07420.1, sup... 427 e-118
gb|AAF79571.1|AC022464_29 F22G5.23 [Arabidopsis thaliana] 427 e-118
gb|AAM64821.1| putative C-4 sterol methyl oxidase [Arabidopsis t... 426 e-118
pir||G84695 probable C-4 sterol methyl oxidase [imported] - Arab... 422 e-117
>gb|AAO13795.1|AF352575_1 putative sterol 4-alpha-methyl-oxidase [Gossypium arboreum]
Length = 269
Score = 449 bits (1155), Expect = e-125
Identities = 208/243 (85%), Positives = 222/243 (90%)
Frame = -2
Query: 960 SFFLHESVFFLSGLPFIWLERAGWMSKYKIQAKNNSSAAQEKCIVRLLIYHFGVNLPVML 781
SFFLHESVFFLSGLPFI+LERAG +SKYKIQ KNNS AAQEKCI RLL+YH GVNLP+M+
Sbjct: 26 SFFLHESVFFLSGLPFIYLERAGLLSKYKIQTKNNSLAAQEKCITRLLLYHIGVNLPLMI 85
Query: 780 LSYPVFKHMGMRSSLPLPSWNIVLTQIIFYFILEDFIFYWGHRILHTKWLYKHVHSVHHE 601
SYP F+ MGM+SSLP PSW +VL+QIIFYFILEDF+FYWGHRILHTKWLYKHVHSVHHE
Sbjct: 86 ASYPFFRFMGMKSSLPFPSWKVVLSQIIFYFILEDFVFYWGHRILHTKWLYKHVHSVHHE 145
Query: 600 YATPFGLTSEYAHPAEILFLGFATIFGPAITGPHLMTLWLWMVLRVLETVEAHSGYHFPW 421
YATPFGLTSEYAHPAEILFLGFATI GPAITGPHL+TLWLWM LRVLETVEAH GYHFPW
Sbjct: 146 YATPFGLTSEYAHPAEILFLGFATIIGPAITGPHLITLWLWMTLRVLETVEAHCGYHFPW 205
Query: 420 SPSNFFPLYGGADFHDYHHRLLYTKSGNYSSTFTYMDRIFGTDVGYRKLKALKNTGVEDI 241
S SNF PLYGGADFHDYHHRLLYTKSGNYSSTF YMD IFGTD GYRKLKALK GVE+
Sbjct: 206 SLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRKLKALKRDGVEEE 265
Query: 240 SKK 232
+K+
Sbjct: 266 AKQ 268
>ref|NP_563789.1| expressed protein; protein id: At1g07420.1, supported by cDNA:
33426., supported by cDNA: gi_16973470 [Arabidopsis
thaliana] gi|16973471|gb|AAL32303.1|AF346734_1 sterol
4-alpha-methyl-oxidase [Arabidopsis thaliana]
Length = 266
Score = 427 bits (1098), Expect = e-118
Identities = 197/236 (83%), Positives = 212/236 (89%)
Frame = -2
Query: 960 SFFLHESVFFLSGLPFIWLERAGWMSKYKIQAKNNSSAAQEKCIVRLLIYHFGVNLPVML 781
SF LHESVFFLSGLPFI+LER G++SKYKIQ KNN+ AAQ KCI RLL+YHF VNLP+ML
Sbjct: 26 SFLLHESVFFLSGLPFIFLERQGFLSKYKIQTKNNTPAAQGKCITRLLLYHFSVNLPLML 85
Query: 780 LSYPVFKHMGMRSSLPLPSWNIVLTQIIFYFILEDFIFYWGHRILHTKWLYKHVHSVHHE 601
SYPVF+ MGMRSS PLPSW V QI+FYFI+EDF+FYWGHRILH+KWLYK+VHSVHHE
Sbjct: 86 ASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIEDFVFYWGHRILHSKWLYKNVHSVHHE 145
Query: 600 YATPFGLTSEYAHPAEILFLGFATIFGPAITGPHLMTLWLWMVLRVLETVEAHSGYHFPW 421
YATPFGLTSEYAHPAEILFLGFATI GPA+TGPHL+TLWLWMVLRVLETVEAH GYHFPW
Sbjct: 146 YATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMVLRVLETVEAHCGYHFPW 205
Query: 420 SPSNFFPLYGGADFHDYHHRLLYTKSGNYSSTFTYMDRIFGTDVGYRKLKALKNTG 253
S SNF PLYGGADFHDYHHRLLYTKSGNYSSTF YMD IFGTD GYR+LK LK G
Sbjct: 206 SLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRRLKTLKENG 261
>gb|AAF79571.1|AC022464_29 F22G5.23 [Arabidopsis thaliana]
Length = 261
Score = 427 bits (1098), Expect = e-118
Identities = 197/236 (83%), Positives = 212/236 (89%)
Frame = -2
Query: 960 SFFLHESVFFLSGLPFIWLERAGWMSKYKIQAKNNSSAAQEKCIVRLLIYHFGVNLPVML 781
SF LHESVFFLSGLPFI+LER G++SKYKIQ KNN+ AAQ KCI RLL+YHF VNLP+ML
Sbjct: 21 SFLLHESVFFLSGLPFIFLERQGFLSKYKIQTKNNTPAAQGKCITRLLLYHFSVNLPLML 80
Query: 780 LSYPVFKHMGMRSSLPLPSWNIVLTQIIFYFILEDFIFYWGHRILHTKWLYKHVHSVHHE 601
SYPVF+ MGMRSS PLPSW V QI+FYFI+EDF+FYWGHRILH+KWLYK+VHSVHHE
Sbjct: 81 ASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIEDFVFYWGHRILHSKWLYKNVHSVHHE 140
Query: 600 YATPFGLTSEYAHPAEILFLGFATIFGPAITGPHLMTLWLWMVLRVLETVEAHSGYHFPW 421
YATPFGLTSEYAHPAEILFLGFATI GPA+TGPHL+TLWLWMVLRVLETVEAH GYHFPW
Sbjct: 141 YATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMVLRVLETVEAHCGYHFPW 200
Query: 420 SPSNFFPLYGGADFHDYHHRLLYTKSGNYSSTFTYMDRIFGTDVGYRKLKALKNTG 253
S SNF PLYGGADFHDYHHRLLYTKSGNYSSTF YMD IFGTD GYR+LK LK G
Sbjct: 201 SLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRRLKTLKENG 256
>gb|AAM64821.1| putative C-4 sterol methyl oxidase [Arabidopsis thaliana]
Length = 266
Score = 426 bits (1094), Expect = e-118
Identities = 196/236 (83%), Positives = 211/236 (89%)
Frame = -2
Query: 960 SFFLHESVFFLSGLPFIWLERAGWMSKYKIQAKNNSSAAQEKCIVRLLIYHFGVNLPVML 781
SF HESVFFLSGLPFI+LER G++SKYKIQ KNN+ AAQ KCI RLL+YHF VNLP+ML
Sbjct: 26 SFLFHESVFFLSGLPFIFLERQGFLSKYKIQTKNNTPAAQGKCITRLLLYHFSVNLPLML 85
Query: 780 LSYPVFKHMGMRSSLPLPSWNIVLTQIIFYFILEDFIFYWGHRILHTKWLYKHVHSVHHE 601
SYPVF+ MGMRSS PLPSW V QI+FYFI+EDF+FYWGHRILH+KWLYK+VHSVHHE
Sbjct: 86 ASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIEDFVFYWGHRILHSKWLYKNVHSVHHE 145
Query: 600 YATPFGLTSEYAHPAEILFLGFATIFGPAITGPHLMTLWLWMVLRVLETVEAHSGYHFPW 421
YATPFGLTSEYAHPAEILFLGFATI GPA+TGPHL+TLWLWMVLRVLETVEAH GYHFPW
Sbjct: 146 YATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMVLRVLETVEAHCGYHFPW 205
Query: 420 SPSNFFPLYGGADFHDYHHRLLYTKSGNYSSTFTYMDRIFGTDVGYRKLKALKNTG 253
S SNF PLYGGADFHDYHHRLLYTKSGNYSSTF YMD IFGTD GYR+LK LK G
Sbjct: 206 SLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRRLKTLKENG 261
>pir||G84695 probable C-4 sterol methyl oxidase [imported] - Arabidopsis
thaliana
Length = 253
Score = 422 bits (1085), Expect = e-117
Identities = 192/235 (81%), Positives = 211/235 (89%)
Frame = -2
Query: 960 SFFLHESVFFLSGLPFIWLERAGWMSKYKIQAKNNSSAAQEKCIVRLLIYHFGVNLPVML 781
SF LHESVFFLSGLP+I+LER G++S YKIQ K+N+ AQ KCI RLL+YH VNLP+M+
Sbjct: 19 SFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPEAQGKCIARLLLYHCCVNLPLMM 78
Query: 780 LSYPVFKHMGMRSSLPLPSWNIVLTQIIFYFILEDFIFYWGHRILHTKWLYKHVHSVHHE 601
SYPVF+ MGM SS PLPSW +V QI+FYFI+EDF+FYWGHRILHTKWLYK+VHSVHHE
Sbjct: 79 ASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIEDFVFYWGHRILHTKWLYKNVHSVHHE 138
Query: 600 YATPFGLTSEYAHPAEILFLGFATIFGPAITGPHLMTLWLWMVLRVLETVEAHSGYHFPW 421
YATPFGLTSEYAHPAEILFLGFATI GPA+TGPHL+TLWLWM+LRV+ETVEAH GYHFPW
Sbjct: 139 YATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMMLRVIETVEAHCGYHFPW 198
Query: 420 SPSNFFPLYGGADFHDYHHRLLYTKSGNYSSTFTYMDRIFGTDVGYRKLKALKNT 256
SPSNF PLYGGADFHDYHHRLLYTKSGNYSSTF YMD IFGTD GYRKLKALK T
Sbjct: 199 SPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRKLKALKET 253
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 850,516,948
Number of Sequences: 1393205
Number of extensions: 19242902
Number of successful extensions: 55329
Number of sequences better than 10.0: 194
Number of HSP's better than 10.0 without gapping: 52725
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 55196
length of database: 448,689,247
effective HSP length: 123
effective length of database: 277,325,032
effective search space used: 54355706272
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
