Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000029A_C01 KMC000029A_c01
(638 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||T07908 4-coumarate-CoA ligase (EC 6.2.1.12) 2 - western bal... 173 2e-42
gb|AAL98709.1|AF279267_1 4-coumarate:coenzyme A ligase [Glycine ... 170 1e-41
pir||T07909 4-coumarate-CoA ligase (EC 6.2.1.12) 1 [validated] -... 169 3e-41
gb|AAF91309.1|AF239686_1 4-coumarate:coA ligase 2 [Rubus idaeus] 165 5e-40
sp|O24540|4CL_VANPL 4-coumarate--CoA ligase (4CL) (4-coumaroyl-C... 162 3e-39
>pir||T07908 4-coumarate-CoA ligase (EC 6.2.1.12) 2 - western balsam poplar x
cottonwood gi|2911797|gb|AAC39365.1| 4-coumarate:CoA
ligase 2 [Populus balsamifera subsp. trichocarpa x
Populus deltoides]
Length = 548
Score = 173 bits (438), Expect = 2e-42
Identities = 91/117 (77%), Positives = 101/117 (85%)
Frame = -3
Query: 636 RLKXLI*SKGFQVAPAELEALLIAHPNISDAAVVPLKDEAAGELPVAFIVRSNGSKITDD 457
RLK LI KGFQVAPAELEA+LIAHPNISDAAVVP+KDEAAGE+PVAF+VRSNGSKIT+D
Sbjct: 436 RLKELIKYKGFQVAPAELEAMLIAHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITED 495
Query: 456 EIKQYISKQVVFYKRINRVFFTDAIPKAASGKILRKELTARLNKDLVAT*NLTCEED 286
EIKQYISKQV+FYKRI RVFFT+AIPKA SGKILRK+L AR V+ +L C D
Sbjct: 496 EIKQYISKQVIFYKRIGRVFFTEAIPKAPSGKILRKDLRAR-----VSAGDLPCTSD 547
>gb|AAL98709.1|AF279267_1 4-coumarate:coenzyme A ligase [Glycine max]
Length = 546
Score = 170 bits (431), Expect = 1e-41
Identities = 89/107 (83%), Positives = 98/107 (91%)
Frame = -3
Query: 636 RLKXLI*SKGFQVAPAELEALLIAHPNISDAAVVPLKDEAAGELPVAFIVRSNGSKITDD 457
RLK LI KGFQVAPAELEALLIAHPNISDAAVV +KDEAAGE+PVAF+VRSNGS+I +D
Sbjct: 437 RLKELIKYKGFQVAPAELEALLIAHPNISDAAVVGMKDEAAGEIPVAFVVRSNGSEIAED 496
Query: 456 EIKQYISKQVVFYKRINRVFFTDAIPKAASGKILRKELTARLNKDLV 316
EIK+YIS+QVVFYKRI RVFFTD+IPKA SGKILRK LTARLN+ LV
Sbjct: 497 EIKKYISQQVVFYKRICRVFFTDSIPKAPSGKILRKVLTARLNEGLV 543
>pir||T07909 4-coumarate-CoA ligase (EC 6.2.1.12) 1 [validated] - western balsam
poplar x cottonwood gi|2911799|gb|AAC39366.1|
4-coumarate:CoA ligase 1 [Populus balsamifera subsp.
trichocarpa x Populus deltoides]
Length = 557
Score = 169 bits (428), Expect = 3e-41
Identities = 85/102 (83%), Positives = 96/102 (93%)
Frame = -3
Query: 636 RLKXLI*SKGFQVAPAELEALLIAHPNISDAAVVPLKDEAAGELPVAFIVRSNGSKITDD 457
RLK LI KGFQVAPAELEA+LIAHP+ISD AVVP+KDEAAGE+P+AF+VR+NGSKIT+D
Sbjct: 437 RLKELIKYKGFQVAPAELEAMLIAHPDISDCAVVPMKDEAAGEVPIAFVVRANGSKITED 496
Query: 456 EIKQYISKQVVFYKRINRVFFTDAIPKAASGKILRKELTARL 331
EIKQYISKQVVFYKRI+RVFFT+AIPKA SGKILRK+L ARL
Sbjct: 497 EIKQYISKQVVFYKRISRVFFTEAIPKAPSGKILRKDLRARL 538
>gb|AAF91309.1|AF239686_1 4-coumarate:coA ligase 2 [Rubus idaeus]
Length = 544
Score = 165 bits (417), Expect = 5e-40
Identities = 83/102 (81%), Positives = 95/102 (92%)
Frame = -3
Query: 636 RLKXLI*SKGFQVAPAELEALLIAHPNISDAAVVPLKDEAAGELPVAFIVRSNGSKITDD 457
RLK LI KGFQVAPAELEA+LI+HPN+SDAAVV +KDEAAGE+PVAF+VRSNGSKI++D
Sbjct: 437 RLKELIKYKGFQVAPAELEAMLISHPNLSDAAVVSMKDEAAGEVPVAFVVRSNGSKISED 496
Query: 456 EIKQYISKQVVFYKRINRVFFTDAIPKAASGKILRKELTARL 331
+IKQYISKQVVFYKRI++VFFTD IPKA SGKILRK+L ARL
Sbjct: 497 DIKQYISKQVVFYKRISKVFFTDKIPKAPSGKILRKDLRARL 538
>sp|O24540|4CL_VANPL 4-coumarate--CoA ligase (4CL) (4-coumaroyl-CoA synthase)
Length = 553
Score = 162 bits (410), Expect = 3e-39
Identities = 82/102 (80%), Positives = 92/102 (89%)
Frame = -3
Query: 636 RLKXLI*SKGFQVAPAELEALLIAHPNISDAAVVPLKDEAAGELPVAFIVRSNGSKITDD 457
RLK LI KGFQVAPAELEALL+ HP ISDAAVVP+KDEAAGE+PVAF+V+SNG IT+D
Sbjct: 444 RLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDEAAGEVPVAFVVKSNGHNITED 503
Query: 456 EIKQYISKQVVFYKRINRVFFTDAIPKAASGKILRKELTARL 331
EIKQ+ISKQV+FYKRINRVFF +AIPKA SGKILRK+L ARL
Sbjct: 504 EIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLRARL 545
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 485,205,911
Number of Sequences: 1393205
Number of extensions: 9769845
Number of successful extensions: 28408
Number of sequences better than 10.0: 1376
Number of HSP's better than 10.0 without gapping: 26671
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27798
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 26723359358
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
