Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KCC001022A_C01 KCC001022A_c01
(890 letters)
Database: nr
1,537,769 sequences; 498,525,298 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q41738|TH41_MAIZE Thiazole biosynthetic enzyme 1-1, chloropla... 160 4e-54
dbj|BAA88227.1| thiamin biosynthetic enzyme [Glycine max] 161 9e-54
ref|NP_011660.1| Protein highly expressed in early stationary ph... 153 9e-54
sp|O23787|THI4_CITSI Thiazole biosynthetic enzyme, chloroplast p... 161 2e-53
dbj|BAA88226.1| thiamin biosynthetic enzyme [Glycine max] 162 2e-53
>sp|Q41738|TH41_MAIZE Thiazole biosynthetic enzyme 1-1, chloroplast precursor
gi|2130146|pir||S61419 thiamin biosynthesis protein
thi1-1 - maize gi|596078|gb|AAA96738.1| thiamine
biosynthetic enzyme
Length = 354
Score = 160 bits (404), Expect(2) = 4e-54
Identities = 84/135 (62%), Positives = 95/135 (70%)
Frame = +3
Query: 483 PAAAPGWAGSCSAAMVVRKPAHEMLDALQVPYEDEGHYVVVRHAALLTSTLMSHVLKNPN 662
P G +AMVVRKPAH LD L V Y++ YVV++HAAL TST+MS +L PN
Sbjct: 125 PGGGAWLGGQLFSAMVVRKPAHLFLDELGVAYDEAEDYVVIKHAALFTSTVMSLLLARPN 184
Query: 663 VKLFNATAVEDLIVTPDPALGPGARRVAGVVTNWSLVAQAHGTQSCMDPNVIEAGVVVSD 842
VKLFNA AVEDLIV RV GVVTNW+LV+ H TQSCMDPNV+EA VVVS
Sbjct: 185 VKLFNAVAVEDLIVR--------GGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 236
Query: 843 CGHDGPFGATGVQRL 887
CGHDGPFGATGV+RL
Sbjct: 237 CGHDGPFGATGVKRL 251
Score = 74.7 bits (182), Expect(2) = 4e-54
Identities = 40/94 (42%), Positives = 56/94 (59%), Gaps = 2/94 (2%)
Frame = +1
Query: 160 SAAPAWWPVWPSPCPPAAAATAAADASMLARAGLPPTTTPYD--DYKFAPIREAEVNRAM 333
S PA P+ A A + A + + PYD ++F+PI+E+ V+R M
Sbjct: 17 SRLPAATRTTPASLVVATGPRGAGAGPICASMSMSSSNPPYDLTSFRFSPIKESIVSREM 76
Query: 334 TRRYFKDMDEFAESDVVIVGAGSAGLACAFELGR 435
TRRY DM +A++DVVIVGAGSAGL+CA+EL +
Sbjct: 77 TRRYMTDMITYADTDVVIVGAGSAGLSCAYELSK 110
Score = 42.0 bits (97), Expect = 0.014
Identities = 17/20 (85%), Positives = 20/20 (100%)
Frame = +2
Query: 461 LMEQSVAPGGGAWLGGQLFS 520
++EQSV+PGGGAWLGGQLFS
Sbjct: 118 IVEQSVSPGGGAWLGGQLFS 137
>dbj|BAA88227.1| thiamin biosynthetic enzyme [Glycine max]
Length = 345
Score = 161 bits (408), Expect(2) = 9e-54
Identities = 83/135 (61%), Positives = 96/135 (70%)
Frame = +3
Query: 483 PAAAPGWAGSCSAAMVVRKPAHEMLDALQVPYEDEGHYVVVRHAALLTSTLMSHVLKNPN 662
P G +AMVVRKPAH LD L V Y+++ +YVV++HAAL TST+MS +L PN
Sbjct: 117 PGGGAWLGGQLFSAMVVRKPAHLFLDELNVEYDEQDNYVVIKHAALFTSTIMSKLLARPN 176
Query: 663 VKLFNATAVEDLIVTPDPALGPGARRVAGVVTNWSLVAQAHGTQSCMDPNVIEAGVVVSD 842
VKLFNA A EDLIV RV GVVTNW+LV+ H TQSCMDPNV+EA VVVS
Sbjct: 177 VKLFNAVAAEDLIVKNG--------RVGGVVTNWALVSLNHDTQSCMDPNVMEAKVVVSS 228
Query: 843 CGHDGPFGATGVQRL 887
CGHDGPFGATGV+RL
Sbjct: 229 CGHDGPFGATGVKRL 243
Score = 72.0 bits (175), Expect(2) = 9e-54
Identities = 38/72 (52%), Positives = 51/72 (70%), Gaps = 2/72 (2%)
Frame = +1
Query: 250 RAGLPPTTTPYD--DYKFAPIREAEVNRAMTRRYFKDMDEFAESDVVIVGAGSAGLACAF 423
RA + + PYD ++F PIRE+ V+R MTRRY DM A++DVVIVGAGSAGL+CA+
Sbjct: 39 RASMSASAPPYDFGSFRFDPIRESIVSREMTRRYMIDMVTHADTDVVIVGAGSAGLSCAY 98
Query: 424 ELGRIAPHLKVA 459
EL + P + +A
Sbjct: 99 ELSK-NPSINIA 109
Score = 42.4 bits (98), Expect = 0.011
Identities = 18/29 (62%), Positives = 23/29 (79%)
Frame = +2
Query: 434 ASRPT*RWLLMEQSVAPGGGAWLGGQLFS 520
+ P+ ++EQSV+PGGGAWLGGQLFS
Sbjct: 101 SKNPSINIAIVEQSVSPGGGAWLGGQLFS 129
>ref|NP_011660.1| Protein highly expressed in early stationary phase during growth on
molasses; Thi4p [Saccharomyces cerevisiae]
gi|417307|sp|P32318|THI4_YEAST Thiazole biosynthetic
enzyme, mitochondrial precursor gi|283204|pir||S25321
thiamin biosynthesis protein thi4 - yeast (Saccharomyces
cerevisiae) gi|4589|emb|CAA43843.1| ESP35 protein
[Saccharomyces cerevisiae] gi|1323242|emb|CAA97157.1|
THI4 [Saccharomyces cerevisiae]
Length = 326
Score = 153 bits (386), Expect(2) = 9e-54
Identities = 85/153 (55%), Positives = 100/153 (64%), Gaps = 19/153 (12%)
Frame = +3
Query: 486 AAAPG---W-AGSCSAAMVVRKPAHEMLDALQVPYEDEGHYVVVRHAALLTSTLMSHVLK 653
+ APG W G +AMV+RKPAH L L++PYEDEG YVVV+HAAL ST++S VL+
Sbjct: 99 SVAPGGGSWLGGQLFSAMVMRKPAHLFLQELEIPYEDEGDYVVVKHAALFISTVLSKVLQ 158
Query: 654 NPNVKLFNATAVEDLIVTPDPALGPGARRVAGVVTNWSLVAQAHGTQSCMDPNVIEA--- 824
PNVKLFNAT VEDL+ P G VAGVVTNW+LV QAHGTQ CMDPNVIE
Sbjct: 159 LPNVKLFNATCVEDLVTRPPTE--KGEVTVAGVVTNWTLVTQAHGTQCCMDPNVIELAGY 216
Query: 825 ------------GVVVSDCGHDGPFGATGVQRL 887
GV++S GHDGPFGA +R+
Sbjct: 217 KNDGTRDLSQKHGVILSTTGHDGPFGAFCAKRI 249
Score = 80.5 bits (197), Expect(2) = 9e-54
Identities = 37/59 (62%), Positives = 49/59 (82%)
Frame = +1
Query: 280 YDDYKFAPIREAEVNRAMTRRYFKDMDEFAESDVVIVGAGSAGLACAFELGRIAPHLKV 456
+ D+KFAPIRE+ V+RAMT RYFKD+D+FA SDV+IVGAGS+GL+ A+ + + P LKV
Sbjct: 35 WSDFKFAPIRESTVSRAMTSRYFKDLDKFAVSDVIIVGAGSSGLSAAYVIAKNRPDLKV 93
Score = 43.5 bits (101), Expect = 0.005
Identities = 19/30 (63%), Positives = 24/30 (79%)
Frame = +2
Query: 431 AASRPT*RWLLMEQSVAPGGGAWLGGQLFS 520
A +RP + ++E SVAPGGG+WLGGQLFS
Sbjct: 85 AKNRPDLKVCIIESSVAPGGGSWLGGQLFS 114
>sp|O23787|THI4_CITSI Thiazole biosynthetic enzyme, chloroplast precursor
gi|7446894|pir||T10474 thiamin biosynthesis protein thi1
- sweet orange gi|2582665|emb|CAB05370.1| thi [Citrus
sinensis]
Length = 356
Score = 161 bits (408), Expect(2) = 2e-53
Identities = 82/135 (60%), Positives = 96/135 (70%)
Frame = +3
Query: 483 PAAAPGWAGSCSAAMVVRKPAHEMLDALQVPYEDEGHYVVVRHAALLTSTLMSHVLKNPN 662
P G +AMVVRKPAH LD L + Y+++ +YVV++HAAL TST+MS +L PN
Sbjct: 126 PGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTIMSKLLARPN 185
Query: 663 VKLFNATAVEDLIVTPDPALGPGARRVAGVVTNWSLVAQAHGTQSCMDPNVIEAGVVVSD 842
VKLFNA A EDLIV RV GVVTNW+LV+ H TQSCMDPNV+EA VVVS
Sbjct: 186 VKLFNAVAAEDLIVK--------GGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 237
Query: 843 CGHDGPFGATGVQRL 887
CGHDGPFGATGV+RL
Sbjct: 238 CGHDGPFGATGVKRL 252
Score = 71.2 bits (173), Expect(2) = 2e-53
Identities = 35/63 (55%), Positives = 50/63 (78%), Gaps = 2/63 (3%)
Frame = +1
Query: 277 PYD--DYKFAPIREAEVNRAMTRRYFKDMDEFAESDVVIVGAGSAGLACAFELGRIAPHL 450
PYD +KF PI+E+ V+R MTRRY DM +A++DVV+VGAGSAGL+CA+EL + P++
Sbjct: 57 PYDLNTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSK-NPNI 115
Query: 451 KVA 459
++A
Sbjct: 116 QIA 118
Score = 42.4 bits (98), Expect = 0.011
Identities = 18/29 (62%), Positives = 23/29 (79%)
Frame = +2
Query: 434 ASRPT*RWLLMEQSVAPGGGAWLGGQLFS 520
+ P + ++EQSV+PGGGAWLGGQLFS
Sbjct: 110 SKNPNIQIAIIEQSVSPGGGAWLGGQLFS 138
>dbj|BAA88226.1| thiamin biosynthetic enzyme [Glycine max]
Length = 349
Score = 162 bits (409), Expect(2) = 2e-53
Identities = 83/135 (61%), Positives = 97/135 (71%)
Frame = +3
Query: 483 PAAAPGWAGSCSAAMVVRKPAHEMLDALQVPYEDEGHYVVVRHAALLTSTLMSHVLKNPN 662
P G +AMVVRKPAH LD L + Y+++ +YVV++HAALLTST+MS +L PN
Sbjct: 121 PGGGAWLGGQLFSAMVVRKPAHLFLDELGLEYDEQDNYVVIKHAALLTSTIMSKLLARPN 180
Query: 663 VKLFNATAVEDLIVTPDPALGPGARRVAGVVTNWSLVAQAHGTQSCMDPNVIEAGVVVSD 842
VKLFNA A EDLIV RV GVVTNW+LV+ H TQSCMDPNV+EA VVVS
Sbjct: 181 VKLFNAVAAEDLIVKNG--------RVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 232
Query: 843 CGHDGPFGATGVQRL 887
CGHDGPFGATGV+RL
Sbjct: 233 CGHDGPFGATGVKRL 247
Score = 70.9 bits (172), Expect(2) = 2e-53
Identities = 40/69 (57%), Positives = 50/69 (71%), Gaps = 2/69 (2%)
Frame = +1
Query: 235 ASMLARAGLPPTTTPYD--DYKFAPIREAEVNRAMTRRYFKDMDEFAESDVVIVGAGSAG 408
ASM A A PP PYD ++F PIRE+ V+R MTRRY DM A++DVV+VGAGSAG
Sbjct: 43 ASMSAAA--PP---PYDFGSFRFEPIRESIVSREMTRRYMTDMVTHADTDVVVVGAGSAG 97
Query: 409 LACAFELGR 435
L+CA+EL +
Sbjct: 98 LSCAYELSK 106
Score = 42.4 bits (98), Expect = 0.011
Identities = 18/29 (62%), Positives = 23/29 (79%)
Frame = +2
Query: 434 ASRPT*RWLLMEQSVAPGGGAWLGGQLFS 520
+ P+ ++EQSV+PGGGAWLGGQLFS
Sbjct: 105 SKNPSVNIAIVEQSVSPGGGAWLGGQLFS 133
