Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005469A_C01 KMC005469A_c01
(717 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_175274.1| leaf development protein Argonaute; protein id:... 155 4e-37
gb|AAF79718.1|AC020889_26 T1N15.2 [Arabidopsis thaliana] 155 5e-37
dbj|BAB96814.1| AGO1 homologous protein [Oryza sativa (japonica ... 152 3e-36
gb|AAO37538.1| putative argonaute protein [Oryza sativa (japonic... 146 3e-34
sp|Q9SJK3|AGOL_ARATH Argonaute-like protein At2g27880 gi|2533583... 145 4e-34
>ref|NP_175274.1| leaf development protein Argonaute; protein id: At1g48410.1,
supported by cDNA: gi_2149639 [Arabidopsis thaliana]
gi|11386626|sp|O04379|AGO1_ARATH Argonaute protein
gi|2149640|gb|AAC18440.1| Argonaute protein [Arabidopsis
thaliana] gi|5733867|gb|AAD49755.1|AC007932_3 Identical
to Argonaute protein from Arabidopsis thaliana
gb|U91995. EST gb|AA720232 comes from this gene
gi|24030354|gb|AAN41341.1| putative leaf development
protein Argonaute [Arabidopsis thaliana]
Length = 1048
Score = 155 bits (393), Expect = 4e-37
Identities = 98/239 (41%), Positives = 138/239 (57%), Gaps = 19/239 (7%)
Frame = +2
Query: 56 RGGRQ--QPDPRRDQPFPTPS----------PHPDA----GTGRGRGRARAPPSQQPPSS 187
RGGR QP P++ Q + P PH G GRG G + PP +Q
Sbjct: 59 RGGRGYGQP-PQQQQQYGGPQEYQGRGRGGPPHQGGRGGYGGGRGGGPSSGPPQRQSVPE 117
Query: 188 SSHSPAPVIGAPISPAPVVSAPISAPSPPPPSPVPVDALSSEVEQ---KLALRPAAPSST 358
+ +P A +S P +S +S P P+ + VEQ A++P PSS+
Sbjct: 118 LHQATSPTYQA-VSSQPTLSE-VSPTQVPEPTVLAQQFEQLSVEQGAPSQAIQPI-PSSS 174
Query: 359 KAIRFPDRPGFGRLGKKIQVRANHFQLQVAERDLHHYDVAITPEITSKKVTREVVSQLIK 538
KA +FP RPG G+ GK+ V+ANHF ++ ++DLHHYDV ITPE+TS+ V R V+ QL+
Sbjct: 175 KAFKFPMRPGKGQSGKRCIVKANHFFAELPDKDLHHYDVTITPEVTSRGVNRAVMKQLVD 234
Query: 539 MYKESVLGNRLPVFDGRKNLFTAGPLPFSSKEFVVKLEDDRPKASSSSGSKKEKRERQF 715
Y++S LG+RLP +DGRK+L+TAGPLPF+SKEF + L D+ G+ ++RER+F
Sbjct: 235 NYRDSHLGSRLPAYDGRKSLYTAGPLPFNSKEFRINLLDE------EVGAGGQRREREF 287
>gb|AAF79718.1|AC020889_26 T1N15.2 [Arabidopsis thaliana]
Length = 1123
Score = 155 bits (392), Expect = 5e-37
Identities = 100/240 (41%), Positives = 137/240 (56%), Gaps = 20/240 (8%)
Frame = +2
Query: 56 RGGRQ--QPDPRRDQPFPTPS----------PHPDA----GTGRGRGRARAPPSQQPPSS 187
RGGR QP P++ Q + P PH G GRG G + PP +Q
Sbjct: 97 RGGRGYGQP-PQQQQQYGGPQEYQGRGRGGPPHQGGRGGYGGGRGGGPSSGPPQRQSVPE 155
Query: 188 SSHSPAPVIGAPISPAPVVSAPISAPSPPPPSPVPVDALSSEVEQ---KLALRPAAPSST 358
+ +P A +S P +S +S P P+ + VEQ A++P PSS+
Sbjct: 156 LHQATSPTYQA-VSSQPTLSE-VSPTQVPEPTVLAQQFEQLSVEQGAPSQAIQPI-PSSS 212
Query: 359 KAIRFPDRPGFGRLGKKIQVRANHFQLQVAERDLHHYDVAITPEITSKKVTREVVSQLIK 538
KA +FP RPG G+ GK+ V+ANHF ++ ++DLHHYDV ITPE+TS+ V R V+ QL+
Sbjct: 213 KAFKFPMRPGKGQSGKRCIVKANHFFAELPDKDLHHYDVTITPEVTSRGVNRAVMKQLVD 272
Query: 539 MYKESVLGNRLPVFDGRKNLFTAGPLPFSSKEFVVKLEDDRPKASSS-SGSKKEKRERQF 715
Y++S LG+RLP +DGRK+L+TAGPLPF+SKEF + L D+ A S S RER+F
Sbjct: 273 NYRDSHLGSRLPAYDGRKSLYTAGPLPFNSKEFRINLLDEEVGAGGQRSSSYGFLREREF 332
>dbj|BAB96814.1| AGO1 homologous protein [Oryza sativa (japonica cultivar-group)]
Length = 909
Score = 152 bits (385), Expect = 3e-36
Identities = 76/154 (49%), Positives = 102/154 (65%)
Frame = +2
Query: 254 ISAPSPPPPSPVPVDALSSEVEQKLALRPAAPSSTKAIRFPDRPGFGRLGKKIQVRANHF 433
++ PSP PP PVDA ++ PSS+K+IRFP RPG G +G + V+ANHF
Sbjct: 9 LAQPSPTPPQEQPVDAATTTPHH-------IPSSSKSIRFPLRPGKGTIGTRCMVKANHF 61
Query: 434 QLQVAERDLHHYDVAITPEITSKKVTREVVSQLIKMYKESVLGNRLPVFDGRKNLFTAGP 613
+ +DLHHYDV+ITPE+TS+ V R V+ +L+ +YK S LG RLP +DGRK+L+TAGP
Sbjct: 62 FAHLPNKDLHHYDVSITPEVTSRIVNRAVIKELVNLYKASYLGGRLPAYDGRKSLYTAGP 121
Query: 614 LPFSSKEFVVKLEDDRPKASSSSGSKKEKRERQF 715
LPF+S+EF + L DD GS E+R+R F
Sbjct: 122 LPFTSQEFQITLLDD------DDGSGSERRQRTF 149
>gb|AAO37538.1| putative argonaute protein [Oryza sativa (japonica cultivar-group)]
Length = 1058
Score = 146 bits (368), Expect = 3e-34
Identities = 104/271 (38%), Positives = 136/271 (49%), Gaps = 48/271 (17%)
Frame = +2
Query: 47 MSRRGGRQQPDPRRDQP------FPTPSPH-------------PDAGTGRGRGRARAPPS 169
M+ R G QP R P P P+ H P G G AR+ P
Sbjct: 53 MTPRPGPPQPQYPRPGPPAVVYGAPMPAAHHQGAYQPGGVYRAPSPGVPVIGGYARSTPV 112
Query: 170 --QQPPSSSSHSPAPVIGAPISPAPVVSAPISAPSPPPPSPVPVDALSSEVEQKL----- 328
+ PP S S +PAP A +PAP S+ +APS AL+ E EQKL
Sbjct: 113 TIRAPPPSHSSAPAPYQPAAAAPAP--SSSSTAPS--------ATALAKEFEQKLFVSET 162
Query: 329 -----------ALRPA-----------APSSTKAIRFPDRPGFGRLGKKIQVRANHFQLQ 442
A PA AP S K + P RPGFG GKK+ +RANHF +
Sbjct: 163 ALAPPAAVASAAAAPAGEASVESDKDLAPVSKKGLAHPARPGFGAAGKKVMIRANHFLVN 222
Query: 443 VAERDLHHYDVAITPEITSKKVTREVVSQLIKMYKESVLGNRLPVFDGRKNLFTAGPLPF 622
VA+ +L HYDV+I PE S+ REV+++LIK++ ++ LG +LP +DGRK+L+TAG LPF
Sbjct: 223 VADNNLFHYDVSINPESKSRATNREVLNELIKLHGKTSLGGKLPAYDGRKSLYTAGSLPF 282
Query: 623 SSKEFVVKLEDDRPKASSSSGSKKEKRERQF 715
S+EFVVKL D K KE+ ER++
Sbjct: 283 ESEEFVVKLIDPEKK-------DKERAEREY 306
>sp|Q9SJK3|AGOL_ARATH Argonaute-like protein At2g27880 gi|25335831|pir||A84678 Argonaute
(AGO1)-like protein [imported] - Arabidopsis thaliana
gi|4510428|gb|AAD21514.1| Argonaute (AGO1)-like protein
[Arabidopsis thaliana]
Length = 997
Score = 145 bits (367), Expect = 4e-34
Identities = 82/181 (45%), Positives = 104/181 (57%)
Frame = +2
Query: 134 GRGRGRARAPPSQQPPSSSSHSPAPVIGAPISPAPVVSAPISAPSPPPPSPVPVDALSSE 313
GRGRG P SS + S V S+ S + +
Sbjct: 81 GRGRGNIGDPTFSVASSSKTVS-------------VASSSKEESKNTEVSETMSNLQITS 127
Query: 314 VEQKLALRPAAPSSTKAIRFPDRPGFGRLGKKIQVRANHFQLQVAERDLHHYDVAITPEI 493
E K + P+S+KA+ FP RPG G LGKK+ VRANHF +QVA+RDL+HYDV+I PE+
Sbjct: 128 TETKPEMTSLPPASSKAVTFPVRPGRGTLGKKVMVRANHFLVQVADRDLYHYDVSINPEV 187
Query: 494 TSKKVTREVVSQLIKMYKESVLGNRLPVFDGRKNLFTAGPLPFSSKEFVVKLEDDRPKAS 673
SK V R V+ L+K YK+S LG + P +DGRK+L+TAGPLPF SKEFVV L + R S
Sbjct: 188 ISKTVNRNVMKLLVKNYKDSHLGGKSPAYDGRKSLYTAGPLPFDSKEFVVNLAEKRADGS 247
Query: 674 S 676
S
Sbjct: 248 S 248
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 677,047,722
Number of Sequences: 1393205
Number of extensions: 18929786
Number of successful extensions: 258201
Number of sequences better than 10.0: 6328
Number of HSP's better than 10.0 without gapping: 106287
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 188651
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 33217548346
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
